tnf alpha  (Hycult Biotech)


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    Hycult Biotech tnf alpha
    Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry <t>(TNF-alpha</t> and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.
    Tnf Alpha, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    tnf alpha - by Bioz Stars, 2024-04
    92/100 stars

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    1) Product Images from "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity"

    Article Title: Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity

    Journal: Journal of Inflammation (London, England)

    doi: 10.1186/1476-9255-8-31

    Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry (TNF-alpha and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.
    Figure Legend Snippet: Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry (TNF-alpha and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.

    Techniques Used: Injection, Staining, Immunohistochemistry

    tnf alpha  (Hycult Biotech)


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    Hycult Biotech tnf alpha
    Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry <t>(TNF-alpha</t> and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.
    Tnf Alpha, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf alpha/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf alpha - by Bioz Stars, 2024-04
    92/100 stars

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    1) Product Images from "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity"

    Article Title: Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity

    Journal: Journal of Inflammation (London, England)

    doi: 10.1186/1476-9255-8-31

    Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry (TNF-alpha and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.
    Figure Legend Snippet: Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry (TNF-alpha and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.

    Techniques Used: Injection, Staining, Immunohistochemistry

    rabbit monoclonal anti tnfα  (Hycult Biotech)


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    Hycult Biotech rabbit monoclonal anti tnfα
    Arthritis progression and the therapeutic effects of CH-DEAE 15 <t>/siRNA-TNFα,</t> folate-PEG-CH-DEAE 15 /siRNA-TNFα and naked siRNA-TNFα nanoparticles in a murine CAIA model. Notes: ( A ) Joint inflammation in mice was measured by an arthritic scoring method to verify disease progression on a scale of 0–4 for each paw, for a total score of 0–16 for all four paws. On the first day (day 1), arthritis was induced by ip injection of a 1.5 mg cocktail of arthritogenic mAb against type II collagen. Two days later (day 3), mice received an ip injection with 50 µg Escherichia coli (0.5 mg/mL stock) lipopolysaccharide. At the same time on days 1, 3, 5 and 7, mice received an ip injection with 100 µL of nanoparticles containing the equivalent of 50 µg siRNA-TNFα. ( B ) Arthritic score on day 10. ( C ) Arthritis development estimated by measuring hind paw thickness over the course of the experiment. ( D ) Hind paw thickness on day 10. ( E ) Hind paws of mice on day 10. Statistical significance was assessed by unpaired Student’s t -test, * P <0.05, *** P <0.001. Each group contained eight mice except group 5 which only had five mice. Group 1: normal control; group 2: CAIA control; group 3: CAIA mice treated with CH-DEAE 15 /siRNA-TNFα nanoparticles; group 4: CAIA mice treated with folate-PEG-CH-DEAE 15 /siRNA-TNFα nanoparticles; group 5: CAIA mice treated with siRNA-TNFα. Abbreviations: CAIA, collagen antibody-induced arthritis; CH, chitosan; DEAE, diethylethylamine; ip, intraperitoneal; <t>mAb,</t> <t>monoclonal</t> antibody; ND, not determined; PEG, polyethylene glycol; SEM, standard error of the means; TNFα, tumor necrosis factor-alpha.
    Rabbit Monoclonal Anti Tnfα, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92/100 stars

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    1) Product Images from "In vivo therapeutic efficacy of TNFα silencing by folate-PEG-chitosan-DEAE/siRNA nanoparticles in arthritic mice"

    Article Title: In vivo therapeutic efficacy of TNFα silencing by folate-PEG-chitosan-DEAE/siRNA nanoparticles in arthritic mice

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S146942

    Arthritis progression and the therapeutic effects of CH-DEAE 15 /siRNA-TNFα, folate-PEG-CH-DEAE 15 /siRNA-TNFα and naked siRNA-TNFα nanoparticles in a murine CAIA model. Notes: ( A ) Joint inflammation in mice was measured by an arthritic scoring method to verify disease progression on a scale of 0–4 for each paw, for a total score of 0–16 for all four paws. On the first day (day 1), arthritis was induced by ip injection of a 1.5 mg cocktail of arthritogenic mAb against type II collagen. Two days later (day 3), mice received an ip injection with 50 µg Escherichia coli (0.5 mg/mL stock) lipopolysaccharide. At the same time on days 1, 3, 5 and 7, mice received an ip injection with 100 µL of nanoparticles containing the equivalent of 50 µg siRNA-TNFα. ( B ) Arthritic score on day 10. ( C ) Arthritis development estimated by measuring hind paw thickness over the course of the experiment. ( D ) Hind paw thickness on day 10. ( E ) Hind paws of mice on day 10. Statistical significance was assessed by unpaired Student’s t -test, * P <0.05, *** P <0.001. Each group contained eight mice except group 5 which only had five mice. Group 1: normal control; group 2: CAIA control; group 3: CAIA mice treated with CH-DEAE 15 /siRNA-TNFα nanoparticles; group 4: CAIA mice treated with folate-PEG-CH-DEAE 15 /siRNA-TNFα nanoparticles; group 5: CAIA mice treated with siRNA-TNFα. Abbreviations: CAIA, collagen antibody-induced arthritis; CH, chitosan; DEAE, diethylethylamine; ip, intraperitoneal; mAb, monoclonal antibody; ND, not determined; PEG, polyethylene glycol; SEM, standard error of the means; TNFα, tumor necrosis factor-alpha.
    Figure Legend Snippet: Arthritis progression and the therapeutic effects of CH-DEAE 15 /siRNA-TNFα, folate-PEG-CH-DEAE 15 /siRNA-TNFα and naked siRNA-TNFα nanoparticles in a murine CAIA model. Notes: ( A ) Joint inflammation in mice was measured by an arthritic scoring method to verify disease progression on a scale of 0–4 for each paw, for a total score of 0–16 for all four paws. On the first day (day 1), arthritis was induced by ip injection of a 1.5 mg cocktail of arthritogenic mAb against type II collagen. Two days later (day 3), mice received an ip injection with 50 µg Escherichia coli (0.5 mg/mL stock) lipopolysaccharide. At the same time on days 1, 3, 5 and 7, mice received an ip injection with 100 µL of nanoparticles containing the equivalent of 50 µg siRNA-TNFα. ( B ) Arthritic score on day 10. ( C ) Arthritis development estimated by measuring hind paw thickness over the course of the experiment. ( D ) Hind paw thickness on day 10. ( E ) Hind paws of mice on day 10. Statistical significance was assessed by unpaired Student’s t -test, * P <0.05, *** P <0.001. Each group contained eight mice except group 5 which only had five mice. Group 1: normal control; group 2: CAIA control; group 3: CAIA mice treated with CH-DEAE 15 /siRNA-TNFα nanoparticles; group 4: CAIA mice treated with folate-PEG-CH-DEAE 15 /siRNA-TNFα nanoparticles; group 5: CAIA mice treated with siRNA-TNFα. Abbreviations: CAIA, collagen antibody-induced arthritis; CH, chitosan; DEAE, diethylethylamine; ip, intraperitoneal; mAb, monoclonal antibody; ND, not determined; PEG, polyethylene glycol; SEM, standard error of the means; TNFα, tumor necrosis factor-alpha.

    Techniques Used: Injection

    tnf  (Hycult Biotech)


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    Hycult Biotech tnf
    Overview Oligonucleotides for qPCR
    Tnf, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Exogenous activation of tumor necrosis factor receptor 2 promotes recovery from sensory and motor disease in a model of multiple sclerosis"

    Article Title: Exogenous activation of tumor necrosis factor receptor 2 promotes recovery from sensory and motor disease in a model of multiple sclerosis

    Journal: Brain, behavior, and immunity

    doi: 10.1016/j.bbi.2019.06.021

    Overview Oligonucleotides for qPCR
    Figure Legend Snippet: Overview Oligonucleotides for qPCR

    Techniques Used:

    tnf  (Hycult Biotech)


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    Hycult Biotech tnf
    Overview Oligonucleotides for qPCR
    Tnf, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92/100 stars

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    1) Product Images from "Exogenous activation of tumor necrosis factor receptor 2 promotes recovery from sensory and motor disease in a model of multiple sclerosis"

    Article Title: Exogenous activation of tumor necrosis factor receptor 2 promotes recovery from sensory and motor disease in a model of multiple sclerosis

    Journal: Brain, behavior, and immunity

    doi: 10.1016/j.bbi.2019.06.021

    Overview Oligonucleotides for qPCR
    Figure Legend Snippet: Overview Oligonucleotides for qPCR

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    anti tnf hp8001 antibody  (Hycult Biotech)


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    Hycult Biotech anti tnf hp8001 antibody
    Anti Tnf Hp8001 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tnfα  (Hycult Biotech)


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    Hycult Biotech tnfα
    Expression <t>of</t> <t>SDF1β</t> and <t>TNFα</t> in the heart. ( a ) SDF1β in LV midsection and ( b ) expression of SDF1β in left and right auricle, LV midsection and apex of the heart in rats 1 day, 2 weeks or 4 weeks after the ligation of LAD compared to sham treated rats. ( c ) SDF1β expression in apex of the heart 2 or 4 weeks after LAD-ligation compared to sham treated rats and ( d ) expression of TNFα in LV midsection and apex 1 day, 2 weeks or 4 weeks after LAD-ligation. N = 5–7 for all groups. Mann–Whitney U test was used for comparison between two groups and Kruskal–Wallis one-way analysis of variance for comparison with multiple groups. *P < 0.05, **P < 0.01.
    Tnfα, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart"

    Article Title: SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-19417-8

    Expression of SDF1β and TNFα in the heart. ( a ) SDF1β in LV midsection and ( b ) expression of SDF1β in left and right auricle, LV midsection and apex of the heart in rats 1 day, 2 weeks or 4 weeks after the ligation of LAD compared to sham treated rats. ( c ) SDF1β expression in apex of the heart 2 or 4 weeks after LAD-ligation compared to sham treated rats and ( d ) expression of TNFα in LV midsection and apex 1 day, 2 weeks or 4 weeks after LAD-ligation. N = 5–7 for all groups. Mann–Whitney U test was used for comparison between two groups and Kruskal–Wallis one-way analysis of variance for comparison with multiple groups. *P < 0.05, **P < 0.01.
    Figure Legend Snippet: Expression of SDF1β and TNFα in the heart. ( a ) SDF1β in LV midsection and ( b ) expression of SDF1β in left and right auricle, LV midsection and apex of the heart in rats 1 day, 2 weeks or 4 weeks after the ligation of LAD compared to sham treated rats. ( c ) SDF1β expression in apex of the heart 2 or 4 weeks after LAD-ligation compared to sham treated rats and ( d ) expression of TNFα in LV midsection and apex 1 day, 2 weeks or 4 weeks after LAD-ligation. N = 5–7 for all groups. Mann–Whitney U test was used for comparison between two groups and Kruskal–Wallis one-way analysis of variance for comparison with multiple groups. *P < 0.05, **P < 0.01.

    Techniques Used: Expressing, Ligation, MANN-WHITNEY

    rabbit monoclonal antitnfα  (Hycult Biotech)


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    Hycult Biotech rabbit monoclonal antitnfα
    Rabbit Monoclonal Antitnfα, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tnfα hp8001  (Hycult Biotech)


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    Hycult Biotech tnfα hp8001
    Antibodies used in the present study
    Tnfα Hp8001, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "miR‐155 induces ROS generation through downregulation of antioxidation‐related genes in mesenchymal stem cells"

    Article Title: miR‐155 induces ROS generation through downregulation of antioxidation‐related genes in mesenchymal stem cells

    Journal: Aging Cell

    doi: 10.1111/acel.12680

    Antibodies used in the present study
    Figure Legend Snippet: Antibodies used in the present study

    Techniques Used: Blocking Assay

    anti tnf hp8001  (Hycult Biotech)


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    Hycult Biotech anti tnf hp8001
    Anti Tnf Hp8001, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hp8001  (Hycult Biotech)


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    Hycult Biotech hp8001
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    Hycult Biotech tnf alpha
    Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry <t>(TNF-alpha</t> and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.
    Tnf Alpha, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf alpha/product/Hycult Biotech
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    tnf alpha - by Bioz Stars, 2024-04
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    Hycult Biotech rabbit monoclonal anti tnfα
    Arthritis progression and the therapeutic effects of CH-DEAE 15 <t>/siRNA-TNFα,</t> folate-PEG-CH-DEAE 15 /siRNA-TNFα and naked siRNA-TNFα nanoparticles in a murine CAIA model. Notes: ( A ) Joint inflammation in mice was measured by an arthritic scoring method to verify disease progression on a scale of 0–4 for each paw, for a total score of 0–16 for all four paws. On the first day (day 1), arthritis was induced by ip injection of a 1.5 mg cocktail of arthritogenic mAb against type II collagen. Two days later (day 3), mice received an ip injection with 50 µg Escherichia coli (0.5 mg/mL stock) lipopolysaccharide. At the same time on days 1, 3, 5 and 7, mice received an ip injection with 100 µL of nanoparticles containing the equivalent of 50 µg siRNA-TNFα. ( B ) Arthritic score on day 10. ( C ) Arthritis development estimated by measuring hind paw thickness over the course of the experiment. ( D ) Hind paw thickness on day 10. ( E ) Hind paws of mice on day 10. Statistical significance was assessed by unpaired Student’s t -test, * P <0.05, *** P <0.001. Each group contained eight mice except group 5 which only had five mice. Group 1: normal control; group 2: CAIA control; group 3: CAIA mice treated with CH-DEAE 15 /siRNA-TNFα nanoparticles; group 4: CAIA mice treated with folate-PEG-CH-DEAE 15 /siRNA-TNFα nanoparticles; group 5: CAIA mice treated with siRNA-TNFα. Abbreviations: CAIA, collagen antibody-induced arthritis; CH, chitosan; DEAE, diethylethylamine; ip, intraperitoneal; <t>mAb,</t> <t>monoclonal</t> antibody; ND, not determined; PEG, polyethylene glycol; SEM, standard error of the means; TNFα, tumor necrosis factor-alpha.
    Rabbit Monoclonal Anti Tnfα, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech tnf
    Overview Oligonucleotides for qPCR
    Tnf, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf/product/Hycult Biotech
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    Hycult Biotech anti tnf hp8001 antibody
    Overview Oligonucleotides for qPCR
    Anti Tnf Hp8001 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tnf hp8001 antibody/product/Hycult Biotech
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    Hycult Biotech tnfα
    Expression <t>of</t> <t>SDF1β</t> and <t>TNFα</t> in the heart. ( a ) SDF1β in LV midsection and ( b ) expression of SDF1β in left and right auricle, LV midsection and apex of the heart in rats 1 day, 2 weeks or 4 weeks after the ligation of LAD compared to sham treated rats. ( c ) SDF1β expression in apex of the heart 2 or 4 weeks after LAD-ligation compared to sham treated rats and ( d ) expression of TNFα in LV midsection and apex 1 day, 2 weeks or 4 weeks after LAD-ligation. N = 5–7 for all groups. Mann–Whitney U test was used for comparison between two groups and Kruskal–Wallis one-way analysis of variance for comparison with multiple groups. *P < 0.05, **P < 0.01.
    Tnfα, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech rabbit monoclonal antitnfα
    Expression <t>of</t> <t>SDF1β</t> and <t>TNFα</t> in the heart. ( a ) SDF1β in LV midsection and ( b ) expression of SDF1β in left and right auricle, LV midsection and apex of the heart in rats 1 day, 2 weeks or 4 weeks after the ligation of LAD compared to sham treated rats. ( c ) SDF1β expression in apex of the heart 2 or 4 weeks after LAD-ligation compared to sham treated rats and ( d ) expression of TNFα in LV midsection and apex 1 day, 2 weeks or 4 weeks after LAD-ligation. N = 5–7 for all groups. Mann–Whitney U test was used for comparison between two groups and Kruskal–Wallis one-way analysis of variance for comparison with multiple groups. *P < 0.05, **P < 0.01.
    Rabbit Monoclonal Antitnfα, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit monoclonal antitnfα - by Bioz Stars, 2024-04
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    Hycult Biotech tnfα hp8001
    Antibodies used in the present study
    Tnfα Hp8001, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tnfα hp8001 - by Bioz Stars, 2024-04
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    Hycult Biotech anti tnf hp8001
    Antibodies used in the present study
    Anti Tnf Hp8001, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti tnf hp8001 - by Bioz Stars, 2024-04
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    Hycult Biotech hp8001
    Antibodies used in the present study
    Hp8001, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hp8001 - by Bioz Stars, 2024-04
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    Image Search Results


    Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry (TNF-alpha and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.

    Journal: Journal of Inflammation (London, England)

    Article Title: Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity

    doi: 10.1186/1476-9255-8-31

    Figure Lengend Snippet: Histological changes induced by the new 4-clone cocktail (CII-3, CII-6, C2B-14, and C2A-12) . DBA/1J mice were injected with the new 4-clone cocktail on day 0 followed by LPS. On day 10, the front paws were amputated for histological examination. The tissues were stained with H&E and for immunohistochemistry (TNF-alpha and IL-1beta). Results shown are representative histological pictures of five mice ankle joints in each group. A: normal paw, B: arthritis, C: normal ankle joint, D: arthritic ankle joint, E: normal TNF- alpha, F: arthritic TNF- alpha, G: normal IL-1 beta, H: arthritic IL-1 beta.

    Article Snippet: The sections were depleted of endogenous peroxidase by incubating in 3% H 2 O 2 in distilled water for 30 min. After blocking non-specific binding with diluted normal rabbit or goat serum in PBS for 20 min, the sections were incubated for 1 h at room temperature with a primary antibody against IL-1beta (SC-1251, goat IgG; Santa Cruz Biotechnology, Santa Cruz, CA) or TNF-alpha (HP8001, rabbit IgG; Hycult Biotechnology BV, Uden, Netherlands).

    Techniques: Injection, Staining, Immunohistochemistry

    Arthritis progression and the therapeutic effects of CH-DEAE 15 /siRNA-TNFα, folate-PEG-CH-DEAE 15 /siRNA-TNFα and naked siRNA-TNFα nanoparticles in a murine CAIA model. Notes: ( A ) Joint inflammation in mice was measured by an arthritic scoring method to verify disease progression on a scale of 0–4 for each paw, for a total score of 0–16 for all four paws. On the first day (day 1), arthritis was induced by ip injection of a 1.5 mg cocktail of arthritogenic mAb against type II collagen. Two days later (day 3), mice received an ip injection with 50 µg Escherichia coli (0.5 mg/mL stock) lipopolysaccharide. At the same time on days 1, 3, 5 and 7, mice received an ip injection with 100 µL of nanoparticles containing the equivalent of 50 µg siRNA-TNFα. ( B ) Arthritic score on day 10. ( C ) Arthritis development estimated by measuring hind paw thickness over the course of the experiment. ( D ) Hind paw thickness on day 10. ( E ) Hind paws of mice on day 10. Statistical significance was assessed by unpaired Student’s t -test, * P <0.05, *** P <0.001. Each group contained eight mice except group 5 which only had five mice. Group 1: normal control; group 2: CAIA control; group 3: CAIA mice treated with CH-DEAE 15 /siRNA-TNFα nanoparticles; group 4: CAIA mice treated with folate-PEG-CH-DEAE 15 /siRNA-TNFα nanoparticles; group 5: CAIA mice treated with siRNA-TNFα. Abbreviations: CAIA, collagen antibody-induced arthritis; CH, chitosan; DEAE, diethylethylamine; ip, intraperitoneal; mAb, monoclonal antibody; ND, not determined; PEG, polyethylene glycol; SEM, standard error of the means; TNFα, tumor necrosis factor-alpha.

    Journal: International Journal of Nanomedicine

    Article Title: In vivo therapeutic efficacy of TNFα silencing by folate-PEG-chitosan-DEAE/siRNA nanoparticles in arthritic mice

    doi: 10.2147/IJN.S146942

    Figure Lengend Snippet: Arthritis progression and the therapeutic effects of CH-DEAE 15 /siRNA-TNFα, folate-PEG-CH-DEAE 15 /siRNA-TNFα and naked siRNA-TNFα nanoparticles in a murine CAIA model. Notes: ( A ) Joint inflammation in mice was measured by an arthritic scoring method to verify disease progression on a scale of 0–4 for each paw, for a total score of 0–16 for all four paws. On the first day (day 1), arthritis was induced by ip injection of a 1.5 mg cocktail of arthritogenic mAb against type II collagen. Two days later (day 3), mice received an ip injection with 50 µg Escherichia coli (0.5 mg/mL stock) lipopolysaccharide. At the same time on days 1, 3, 5 and 7, mice received an ip injection with 100 µL of nanoparticles containing the equivalent of 50 µg siRNA-TNFα. ( B ) Arthritic score on day 10. ( C ) Arthritis development estimated by measuring hind paw thickness over the course of the experiment. ( D ) Hind paw thickness on day 10. ( E ) Hind paws of mice on day 10. Statistical significance was assessed by unpaired Student’s t -test, * P <0.05, *** P <0.001. Each group contained eight mice except group 5 which only had five mice. Group 1: normal control; group 2: CAIA control; group 3: CAIA mice treated with CH-DEAE 15 /siRNA-TNFα nanoparticles; group 4: CAIA mice treated with folate-PEG-CH-DEAE 15 /siRNA-TNFα nanoparticles; group 5: CAIA mice treated with siRNA-TNFα. Abbreviations: CAIA, collagen antibody-induced arthritis; CH, chitosan; DEAE, diethylethylamine; ip, intraperitoneal; mAb, monoclonal antibody; ND, not determined; PEG, polyethylene glycol; SEM, standard error of the means; TNFα, tumor necrosis factor-alpha.

    Article Snippet: Immunohistochemistry included rabbit monoclonal anti-TNFα (diluted 1:1,000; HP8001, rabbit IG; Hycult Biotechnology BV, Uden, the Netherlands).

    Techniques: Injection

    Overview Oligonucleotides for qPCR

    Journal: Brain, behavior, and immunity

    Article Title: Exogenous activation of tumor necrosis factor receptor 2 promotes recovery from sensory and motor disease in a model of multiple sclerosis

    doi: 10.1016/j.bbi.2019.06.021

    Figure Lengend Snippet: Overview Oligonucleotides for qPCR

    Article Snippet: Other antibodies included APP (Novus Biologicals, Littleton, CO), Iba1 (Wako Pure Chemicals, Osaka, Japan), CD3 (Novus Biologicals, Littleton, CO), FoxP3 (Abeam, Cambridge, UK), Human IgE (Novus Biologicals, NB7456) and TNF (HP8001, Hycult Biotech, The Netherlands).

    Techniques:

    Expression of SDF1β and TNFα in the heart. ( a ) SDF1β in LV midsection and ( b ) expression of SDF1β in left and right auricle, LV midsection and apex of the heart in rats 1 day, 2 weeks or 4 weeks after the ligation of LAD compared to sham treated rats. ( c ) SDF1β expression in apex of the heart 2 or 4 weeks after LAD-ligation compared to sham treated rats and ( d ) expression of TNFα in LV midsection and apex 1 day, 2 weeks or 4 weeks after LAD-ligation. N = 5–7 for all groups. Mann–Whitney U test was used for comparison between two groups and Kruskal–Wallis one-way analysis of variance for comparison with multiple groups. *P < 0.05, **P < 0.01.

    Journal: Scientific Reports

    Article Title: SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart

    doi: 10.1038/s41598-018-19417-8

    Figure Lengend Snippet: Expression of SDF1β and TNFα in the heart. ( a ) SDF1β in LV midsection and ( b ) expression of SDF1β in left and right auricle, LV midsection and apex of the heart in rats 1 day, 2 weeks or 4 weeks after the ligation of LAD compared to sham treated rats. ( c ) SDF1β expression in apex of the heart 2 or 4 weeks after LAD-ligation compared to sham treated rats and ( d ) expression of TNFα in LV midsection and apex 1 day, 2 weeks or 4 weeks after LAD-ligation. N = 5–7 for all groups. Mann–Whitney U test was used for comparison between two groups and Kruskal–Wallis one-way analysis of variance for comparison with multiple groups. *P < 0.05, **P < 0.01.

    Article Snippet: SDF1α (5388, BioVision), SDF1β (ab25118, Abcam) and TNFα (HP8001, HyCult biotechnology) were used to detect the cytokine expression in myocardium.

    Techniques: Expressing, Ligation, MANN-WHITNEY

    Antibodies used in the present study

    Journal: Aging Cell

    Article Title: miR‐155 induces ROS generation through downregulation of antioxidation‐related genes in mesenchymal stem cells

    doi: 10.1111/acel.12680

    Figure Lengend Snippet: Antibodies used in the present study

    Article Snippet: Tnfα (HP8001) , Hycult Biotech , WB , 1/1000 in Immuno‐enhancer.

    Techniques: Blocking Assay