tnf alpha cdna orf clone in cloning vector human  (Sino Biological)


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    Name:
    TNF alpha cDNA ORF Clone in Cloning Vector Human
    Description:
    Full length Clone DNA of Human tumor necrosis factor TNF superfamily member 2
    Catalog Number:
    HG10602-M
    Price:
    75.0
    Category:
    cDNA Clone
    Size:
    1Unit
    Product Aliases:
    DIF cDNA ORF Clone Human, TNF-alpha cDNA ORF Clone Human, TNFA cDNA ORF Clone Human, TNFSF2 cDNA ORF Clone Human
    Molecule Name:
    TNF,TNFSF2,TNF-alpha,
    Buy from Supplier


    Structured Review

    Sino Biological tnf alpha cdna orf clone in cloning vector human
    Full length Clone DNA of Human tumor necrosis factor TNF superfamily member 2
    https://www.bioz.com/result/tnf alpha cdna orf clone in cloning vector human/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf alpha cdna orf clone in cloning vector human - by Bioz Stars, 2021-05
    91/100 stars

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    Related Articles

    Staining:

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. Toluidine blue staining SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

    Transfection:

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. Toluidine blue staining SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

    Incubation:

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. Toluidine blue staining SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

    Plasmid Preparation:

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. Toluidine blue staining SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis
    Article Snippet: The expression of exogenous HS6ST2 was determined by anti-FLAG antibody (1:1000, Sigma, catalog F1804) in western blotting. .. SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection). .. After washing with PBS buffer, the results of staining were analyzed by photography under microscope with 200× magnification and quantification with the Image-Pro® Plus software.

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    Sino Biological tnf α
    MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under <t>TNF-α</t> treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value
    Tnf α, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α - by Bioz Stars, 2021-05
    91/100 stars
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    MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under TNF-α treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: MiR-23b-3p could enhance matrix degradation by means of regulating activity of p38 MAPK in human chondrocytes under TNF-α treatment. a , b SW1353 cells were transfected by mimic miR-23b-3p ( a ) or anti-miR-23b-3p sequence ( b ) with or without TNF-α for 24 h, and the protein expression of phosphorylation form of p38 MAPK (p-p38), total p38 MAPK, and MMP13 was detected by western blotting. Asterisk (*): compared with the mimic NC ( a ) or anti-NC group ( b ). c SW1353 cells were treated with mimic miR-23b-3p with or without p38 MAPK inhibitor SB203580 (10 μM) under stimulation of TNF-α. The protein expression of MMP13 was determined by western blotting. Asterisk (*): compared with the mimic NC group. d Interfering efficiency of siRNAs against p38 MAPK was determined by western blotting under 50 nM p38 siRNA, 50 nM negative control (NC), and Mock (transfection regent only) transfection for 48 h. e Under stimulation with TNF-α, SW1353 cells were transfected by mimic miR-23b-3p under treatment with si- p38 mixture (containing three siRNA target sequences), and p-p38, total p38, and MMP13 were determined by western blotting. Asterisk (*): compared with mimic NC or si-NC. Each relative expression of phosphorylation form was normalized by the total form. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection).

    Techniques: Activity Assay, Transfection, Sequencing, Expressing, Western Blot, Negative Control, MANN-WHITNEY

    HS6ST2 could regulate the matrix degradation depending on the activity of p38 MAPK. a SW1353 cells were transfected by si -HS6ST2 or negative control (si-NC) with or without TNF-α for 24 h, and the protein expression of p-p38 and p38 was detected by western blotting. Asterisk (*): compared with the si-NC group. b Under stimulation with TNF-α, SW1353 cells were transfected by empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) under treatment with mimic miR-23b-3p, and p-p38 and total p38 were determined by western blotting. Asterisk (*): compared with the mimic NC group. c SW1353 cells were transfected with HS6ST2 siRNA with or without p38 MAPK inhibitor SB203580 (10 μM) after treatment of TNF-α, and the protein expression of MMP13 was determined by western blotting. Each relative expression of phosphorylation form was normalized by the total form. d Schematic representation of miR-23b-3p–HS6ST2 axis-mediated catabolic effects in human chondrocyte. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: HS6ST2 could regulate the matrix degradation depending on the activity of p38 MAPK. a SW1353 cells were transfected by si -HS6ST2 or negative control (si-NC) with or without TNF-α for 24 h, and the protein expression of p-p38 and p38 was detected by western blotting. Asterisk (*): compared with the si-NC group. b Under stimulation with TNF-α, SW1353 cells were transfected by empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) under treatment with mimic miR-23b-3p, and p-p38 and total p38 were determined by western blotting. Asterisk (*): compared with the mimic NC group. c SW1353 cells were transfected with HS6ST2 siRNA with or without p38 MAPK inhibitor SB203580 (10 μM) after treatment of TNF-α, and the protein expression of MMP13 was determined by western blotting. Each relative expression of phosphorylation form was normalized by the total form. d Schematic representation of miR-23b-3p–HS6ST2 axis-mediated catabolic effects in human chondrocyte. GAPDH was used as internal controls in western blotting detection. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection).

    Techniques: Activity Assay, Transfection, Negative Control, Expressing, Western Blot, Plasmid Preparation, MANN-WHITNEY

    MiR-23b-3p could enhance matrix degradation in human chondrocytes via regulating HS6ST2. a , b SW1353 cells transfected with 50 nM HS6ST2 siRNA mixture (containing three target sequences, si- HS6ST2 ) or negative control (si-NC) were stimulated by 10 ng/ml TNF-α for 24 h. Protein level of HS6ST2 and MMP13 were assayed by western blotting ( a ) and matrix content of chondrocytes was determined by toluidine blue staining ( b ). Asterisk (*): compared with the si-NC group. c , d SW1353 cells transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for 24 h were stimulated by TNF-α for another 24 h. The protein expression of MMP13 was assayed by western blotting ( c ) and matrix content of chondrocytes was determined by toluidine blue staining ( d ). Asterisk (*): compared with the FLAG-Ctrl group. e , f Under stimulation with TNF-α, SW1353 cells were treated with mimic NC or mimic miR-23b-3p for 24 h and then transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for another 24 h to observe the rescuing effect of mimic miR-23b-3p in MMP13 expression ( e ) and matrix content ( f ). Asterisk (*): compared with the mimic NC group. GAPDH was used as internal controls in western blotting detection. In toluidine blue staining results, scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Bars represent standard error of the mean (SEM) from three independent experiments. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: MiR-23b-3p could enhance matrix degradation in human chondrocytes via regulating HS6ST2. a , b SW1353 cells transfected with 50 nM HS6ST2 siRNA mixture (containing three target sequences, si- HS6ST2 ) or negative control (si-NC) were stimulated by 10 ng/ml TNF-α for 24 h. Protein level of HS6ST2 and MMP13 were assayed by western blotting ( a ) and matrix content of chondrocytes was determined by toluidine blue staining ( b ). Asterisk (*): compared with the si-NC group. c , d SW1353 cells transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for 24 h were stimulated by TNF-α for another 24 h. The protein expression of MMP13 was assayed by western blotting ( c ) and matrix content of chondrocytes was determined by toluidine blue staining ( d ). Asterisk (*): compared with the FLAG-Ctrl group. e , f Under stimulation with TNF-α, SW1353 cells were treated with mimic NC or mimic miR-23b-3p for 24 h and then transfected with empty vector (FLAG-Ctrl) or pcDNA3.1-FLAG- HS6ST2 vector (FLAG- HS6ST2 ) for another 24 h to observe the rescuing effect of mimic miR-23b-3p in MMP13 expression ( e ) and matrix content ( f ). Asterisk (*): compared with the mimic NC group. GAPDH was used as internal controls in western blotting detection. In toluidine blue staining results, scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Bars represent standard error of the mean (SEM) from three independent experiments. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection).

    Techniques: Transfection, Negative Control, Western Blot, Staining, Plasmid Preparation, Expressing, MANN-WHITNEY

    TNF-α-treated chondrocytes increases matrix degradation regulated by miR-23b-3p. a Stem-loop RT-qPCR result of miR-23b-3p (left panel) and protein levels of HS6ST2 and MMP13 (right panel) were assayed in SW1353 cells under stimulation by 10 ng/ml TNF-α for 24 h. b , c Western blotting result of HS6ST2 and MMP13 protein in SW1353 cells transfected with 10 nM mimic miR-23b-3p ( b ) or 50 nM anti-miR-23b-3p sequence ( c ) and stimulated by 10 ng/ml TNF-α for 24 h. d , e Toluidine blue staining results of SW1353 cells transfected with 10 nM mimic miR-23b-3p ( d ) or 50 nM anti-miR-23b-3p sequence ( e ) and stimulated by 10 ng/ml TNF-α for 24 h. Scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Asterisk (*): compared with mimic NC or anti-NC group. U6 snRNA and GAPDH were used as internal controls in RT-qPCR for miRNA and western blotting detection, respectively. Bars represent standard error of the mean (SEM) from three independent experiments. One representative result and quantitative data from three independent western blotting and toluidine blue staining. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Journal: Cell Death & Disease

    Article Title: Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis

    doi: 10.1038/s41419-018-0729-0

    Figure Lengend Snippet: TNF-α-treated chondrocytes increases matrix degradation regulated by miR-23b-3p. a Stem-loop RT-qPCR result of miR-23b-3p (left panel) and protein levels of HS6ST2 and MMP13 (right panel) were assayed in SW1353 cells under stimulation by 10 ng/ml TNF-α for 24 h. b , c Western blotting result of HS6ST2 and MMP13 protein in SW1353 cells transfected with 10 nM mimic miR-23b-3p ( b ) or 50 nM anti-miR-23b-3p sequence ( c ) and stimulated by 10 ng/ml TNF-α for 24 h. d , e Toluidine blue staining results of SW1353 cells transfected with 10 nM mimic miR-23b-3p ( d ) or 50 nM anti-miR-23b-3p sequence ( e ) and stimulated by 10 ng/ml TNF-α for 24 h. Scale bar, 100 μm. Lower panel, statistical analysis of average optical density of matrix staining of toluidine blue. Asterisk (*): compared with mimic NC or anti-NC group. U6 snRNA and GAPDH were used as internal controls in RT-qPCR for miRNA and western blotting detection, respectively. Bars represent standard error of the mean (SEM) from three independent experiments. One representative result and quantitative data from three independent western blotting and toluidine blue staining. Mann–Whitney U test was used to identify statistical differences between two groups. Asterisk (*) or hash ( # ) stands for P value

    Article Snippet: SW1353 cells were seeded in 24-well plates at 3 × 104 for 24 h. After transfection of miRNAs or plasmids, the chondrocytes were incubated with TNF-α (10 ng/ml, Sino Biological lnc, catalog HG10602-M) for another 24 h. In order to determine the matrix content, the chondrocytes were fixed in 4% buffered paraformaldehyde for at least 20 min and stained with 1% toluidine blue for 10 min (miRNA or siRNA transfection) or 15 min (plasmid transfection).

    Techniques: Quantitative RT-PCR, Western Blot, Transfection, Sequencing, Staining, MANN-WHITNEY