Journal: iScience

Article Title: A randomized clinical trial of bermekimab treatment for clinical improvement of systemic sclerosis

doi: 10.1016/j.isci.2023.107670

Figure Lengend Snippet:

Article Snippet: Human TNF-α uncoated ELISA Kit , Diaclone , EL:1100-34.

Techniques: Recombinant, Saline, Enzyme-linked Immunosorbent Assay, Software

Journal: Cureus

Article Title: Correlation Between Serum Tumor Necrosis Factor-Alpha (TNF-α) and Clinical Severity of Tuberculosis: A Hospital-Based Study

doi: 10.7759/cureus.35626

Figure Lengend Snippet: Demographic and risk factors in TB cases and controls of the study. PFT: pulmonary function test, TNF-α: tumor necrosis factor-alpha

Article Snippet: The sensitivity or the minimum detectable dose of TNF-α using this high-sensitivity diaclone TNF-α ELISA kit was found to be 0.5 pg/mL.

Techniques:

Journal: Cureus

Article Title: Correlation Between Serum Tumor Necrosis Factor-Alpha (TNF-α) and Clinical Severity of Tuberculosis: A Hospital-Based Study

doi: 10.7759/cureus.35626

Figure Lengend Snippet: TNF-α: tumor necrosis factor-alpha

Article Snippet: The sensitivity or the minimum detectable dose of TNF-α using this high-sensitivity diaclone TNF-α ELISA kit was found to be 0.5 pg/mL.

Techniques:

Journal: Cureus

Article Title: Correlation Between Serum Tumor Necrosis Factor-Alpha (TNF-α) and Clinical Severity of Tuberculosis: A Hospital-Based Study

doi: 10.7759/cureus.35626

Figure Lengend Snippet: Correlation between TNF-α levels and smoking and positive history of TB among blood relatives in relation to TB development. TNF-α: tumor necrosis factor-alpha; TB: tuberculosis

Article Snippet: The sensitivity or the minimum detectable dose of TNF-α using this high-sensitivity diaclone TNF-α ELISA kit was found to be 0.5 pg/mL.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ganoderma lucidum Protects Dopaminergic Neuron Degeneration through Inhibition of Microglial Activation

doi: 10.1093/ecam/nep075

Figure Lengend Snippet:

Article Snippet: Diaclone (Besancon, France) supplied Rat TNF- α detection ELISA kits, while superoxide Assay Kit-WST and rat IL-1 β ELISA kits were obtained from Dojindo (Kyushu, Japan) and IBL (Gunma, Japan), respectively.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ganoderma lucidum Protects Dopaminergic Neuron Degeneration through Inhibition of Microglial Activation

doi: 10.1093/ecam/nep075

Figure Lengend Snippet: LPS and CF increase cytokines through activating microglia. Microglial activation was determined by measuring the levels of TNF- α , IL-1 β , NO and superoxide in cells after exposure to LPS (0.25 μ g/ml) and CF (150 μ g/ml). The levels of TNF- α , IL-1 β , NO and superoxide in vehicle controls are 106.55 pg/ml, 119.09 pg/ml, 0.6 μ M and 5.22 U/ml, respectively. Levels were expressed as fold increase as compared to concentrations of the control. All cytokines were increased significantly (* P < .05).

Article Snippet: Diaclone (Besancon, France) supplied Rat TNF- α detection ELISA kits, while superoxide Assay Kit-WST and rat IL-1 β ELISA kits were obtained from Dojindo (Kyushu, Japan) and IBL (Gunma, Japan), respectively.

Techniques: Activation Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ganoderma lucidum Protects Dopaminergic Neuron Degeneration through Inhibition of Microglial Activation

doi: 10.1093/ecam/nep075

Figure Lengend Snippet: GL protects against LPS or CF induced production of TNF- α (a) and IL-1 β (b) in a dose-dependent fashion. Cultures were treated with GL at indicated concentration 30 min prior to exposure with 0.25 μ g/ml LPS or 150 μ g/ml CF. TNF- α and IL-1 β levels were determined as described in Methods section. Data are expressed as means ± SD of two experiments performed in triplicate. * P < .05 and ** P < .001 compared with LPS and CF only treated cultures for TNF- α . # P < .001 and ## P < .001 compared with LPS and CF only treated cultures for IL-1 β , respectively.

Article Snippet: Diaclone (Besancon, France) supplied Rat TNF- α detection ELISA kits, while superoxide Assay Kit-WST and rat IL-1 β ELISA kits were obtained from Dojindo (Kyushu, Japan) and IBL (Gunma, Japan), respectively.

Techniques: Concentration Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Ganoderma lucidum Protects Dopaminergic Neuron Degeneration through Inhibition of Microglial Activation

doi: 10.1093/ecam/nep075

Figure Lengend Snippet: GL protects against LPS or CF induced overexpression of mRNA levels of TNF- α (a) and IL-1 β (b) in a dose-dependent fashion. Cultures were treated with GL at indicated concentration 30 min prior to exposure with 0.25 μ g/ml LPS or 150 μ g/ml CF. Total RNA was extracted and then subjected to real-time PCR as described in Methods section. Data are expressed as percentage of the control group (LPS or CF only treated group, respectively) calculated from the average threshold cycle values and presented as the mean ± SD. Independent RNA preparations from different sets of cultures were prepared and determinations were performed in triplicate from the RNA samples of a set of experiment. * P < .05 compared with LPS or CF only treated cultures, respectively.

Article Snippet: Diaclone (Besancon, France) supplied Rat TNF- α detection ELISA kits, while superoxide Assay Kit-WST and rat IL-1 β ELISA kits were obtained from Dojindo (Kyushu, Japan) and IBL (Gunma, Japan), respectively.

Techniques: Over Expression, Concentration Assay, Real-time Polymerase Chain Reaction

Journal: Open Biology

Article Title: IFNγ-dependent silencing of TFF1 during Helicobacter pylori infection

doi: 10.1098/rsob.220278

Figure Lengend Snippet: TFF1 expression is downregulated by IFNγ. ( a ) Real-time PCR analysis of TFF1 in KATO III cells after 24 h incubation with TNFα (40 ng ml −1 ), IFNγ (10 ng ml −1 ) and IL1β (40 ng ml −1 ) alone or in combination (mix). HPRT1 was used as a housekeeping gene. ( b , c ) Western blot analysis of intracellular TFF1 protein in KATO III cell line upon the above-described treatments and densitometric analysis of TFF1 signals ( b ) normalized to β-actin. ( d , e ) Western blot analysis of secreted TFF1 protein from the supernatants of KATO III treated as ( a ) and the densitometric analysis of TFF1. Images are representative of at least three independent experiments. Data are expressed as mean ± s.d. ( t -test, ** p -value ≤ 0.01, *** p -value ≤ 0.001), and the mean of the controls was set to 1.

Article Snippet: Supernatants were collected for the quantification of the following cytokines by ELISA according to the manufacturer's instructions: IFNγ (Invitrogen, no. 88-7316-88, USA), IL6 (Diaclone SAS, no. 950.030.192, France), TNFα (Diaclone SAS, no. 950.090.192, France) and IL1β (Diaclone SAS, no. 850.006.192, France).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Incubation, Western Blot

Journal: Open Biology

Article Title: IFNγ-dependent silencing of TFF1 during Helicobacter pylori infection

doi: 10.1098/rsob.220278

Figure Lengend Snippet: IFNγ reduces luciferase activity under TFF1 promoter control. ( a ) Luciferase reporter assays in KATO III cells transfected with pGL3-1kb-Luc plasmids, containing a fragment starting from −931 bp of TFF1 promoter upstream of a luciferase reporter gene and incubated with TNFα (40 ng ml −1 ), IFNγ (10 ng ml −1 ) and IL1β (40 ng ml −1 ) alone or in combination (mix). ( b ) Luciferase reporter assays in KATO III cells transfected with different plasmids containing fragments of various lengths from −931 bp to −212 bp and stimulated with IFNγ for 24 h. Data are expressed as the mean of four independent experiments ± s.d. ( t -test, ** p -value ≤ 0.01, *** p -value ≤ 0.001), the mean of pGL3-0.2kb-Luc plasmid was set to 1.

Article Snippet: Supernatants were collected for the quantification of the following cytokines by ELISA according to the manufacturer's instructions: IFNγ (Invitrogen, no. 88-7316-88, USA), IL6 (Diaclone SAS, no. 950.030.192, France), TNFα (Diaclone SAS, no. 950.090.192, France) and IL1β (Diaclone SAS, no. 850.006.192, France).

Techniques: Luciferase, Activity Assay, Transfection, Incubation, Plasmid Preparation

Journal: Open Biology

Article Title: IFNγ-dependent silencing of TFF1 during Helicobacter pylori infection

doi: 10.1098/rsob.220278

Figure Lengend Snippet: TFF1 expression in mucosoids is affected by cytokines during Helicobacter infection. ( a ) Immunofluorescence analysis of TFF1 (green) in ‘foveola’ (without Wnt3A/RSPO1, −W−R, a – c ) and in ‘basal' cells (with Wnt3A/RSPO1, +W +R, d­ – f ) of mucosoids. Scale bars: 10 µm. ( b ) TFF1 and ( c ) IL8 expression level in mucosoids after 1, 3 and 5 days post H. pylori infection. HPRT1 was used as a housekeeping gene. Data are expressed as the mean of three independent experiments ± SD ( t -test, ** p -value ≤ 0.01, **** p -value ≤ 0.0001). ( d ) Microarray analysis of TFF1, TFF2 and TFF3 in mucosoids in +W +R conditions exposed to the indicated cytokines for 5 days. ( e , f ) Mucosoids were infected with H. pylori at MOI 50 and, the day after, exposed to the mixture of cytokines (TNFα, IL1β and IFNγ) for 5 days. ( e ) TFF1 and ( f ) IL8 transcripts were measured by RTqPCR. HPRT1 served as a housekeeping gene. Data are expressed as the mean of three independent experiments ± s.d. ( t -test, ** p -value ≤ 0.01, *** p -value ≤ 0.001).

Article Snippet: Supernatants were collected for the quantification of the following cytokines by ELISA according to the manufacturer's instructions: IFNγ (Invitrogen, no. 88-7316-88, USA), IL6 (Diaclone SAS, no. 950.030.192, France), TNFα (Diaclone SAS, no. 950.090.192, France) and IL1β (Diaclone SAS, no. 850.006.192, France).

Techniques: Expressing, Infection, Immunofluorescence, Microarray

Journal: BMC Complementary and Alternative Medicine

Article Title: Anti-Neuroinflammatory effects of the extract of Achillea fragrantissima

doi: 10.1186/1472-6882-11-98

Figure Lengend Snippet: Down-regulation of TNFα secretion from activated microglial cells by Af extract . Microglial cells were treated with the indicated concentrations of Af extract, followed by stimulation with LPS (100 ng/mL). After 5 h, conditioned media were collected and tested for cytokine levels by ELISA. TNFα levels in the activated cells (designated as 100%) were 900 pg/mL. Data represent the means ± SEM of two independent experiments (n = 4). * p < 0.01; ** p < 0.001.

Article Snippet: Five hours later, conditioned media from duplicate wells per sample were collected and tested for cytokine levels with a rat TNFα ELISA kit (Diaclone ® ; Gen-Probe Life Sciences Ltd. France), used according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: BioMed Research International

Article Title: Demethylation of Cancer/Testis Antigens and CpG ODN Stimulation Enhance Dendritic Cell and Cytotoxic T Lymphocyte Function in a Mouse Mammary Model

doi: 10.1155/2013/196894

Figure Lengend Snippet: The effect of P1A-specific peptide and CpG ODN on DC maturation. (a) DCs positive for CD80, CD86, and CD11c by flow cytometry. (b) ELISA measurements of IL-6 and TNF- α release by cultured DCs. IL-4 and GM-CSF, P1A-specific peptide, CpG ODN, or P1A-specific peptide + CpG ODN were added to DCs for 96 hour. Values are the means ± SD ( n = 3) of three individual experiments. * P < 0.05 and ** P < 0.01 versus control; # P < 0.05 and ## P < 0.01 versus group treated with P1A-specific peptide or CpG ODN.

Article Snippet: Supernatant IL-6 and TNF- α levels were assayed using a mouse IL-6 enzyme-linked immunosorbent assay (ELISA) kit and a mouse TNF- α ELISA kit (Diaclone, Besançon, France) according to the manufacturer's instructions.

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture