tnfα  (Thermo Fisher)


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    Name:
    TNF alpha Monoclonal Antibody MAb11
    Description:
    TNF alpha Monoclonal Antibody for Flow
    Catalog Number:
    11-7349-41
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher tnfα
    Atypical Granzyme B + IFNγ low <t>TNFα</t> low CD3 + CD8 dim T cells arise in school-age children living in areas of high pathogen burden.
    TNF alpha Monoclonal Antibody for Flow
    https://www.bioz.com/result/tnfα/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfα - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "High pathogen burden in childhood promotes the development of unconventional innate-like CD8+ T cells"

    Article Title: High pathogen burden in childhood promotes the development of unconventional innate-like CD8+ T cells

    Journal: JCI Insight

    doi: 10.1172/jci.insight.93814

    Atypical Granzyme B + IFNγ low TNFα low CD3 + CD8 dim T cells arise in school-age children living in areas of high pathogen burden.
    Figure Legend Snippet: Atypical Granzyme B + IFNγ low TNFα low CD3 + CD8 dim T cells arise in school-age children living in areas of high pathogen burden.

    Techniques Used:

    Related Articles

    Staining:

    Article Title: High pathogen burden in childhood promotes the development of unconventional innate-like CD8+ T cells
    Article Snippet: Intracellular staining was performed following manufacturer instructions from fixation/permeabilization concentrate and diluent (eBiosciences). .. Surface-stained cells were fixed for 30 minutes at 4°C, washed twice in cold washing buffer, and stained 30 minutes at 4°C with the following antibodies: IFNγ (Alexa-Fluor 780, 4S.B3, eBiosciences), Granzyme B (PE, Invitrogen), KLF2 (PE, R & D Systems), NFAT1 (PE, 1:100, 14335, Cell Signaling Technologies), T-bet (FITC, eBio4B10, eBiosciences), Eomes (eFluor 660, WD1928, eBiosciences), TNFα (FITC, MAb11, eBiosciences). .. For the proliferation assay, sorted subsets of PBMCs were labeled with 1 M CFSE (Invitrogen) in PBS at 37°C for 8 minutes.

    Article Title: Signaling Lymphocyte Activated Molecule (SLAM)/SLAM-associated Protein (SAP) Pathway Regulates Human B-cell Tolerance
    Article Snippet: Intracellular staining for FOXP3-AlexaFluor488 (clone PCH101, eBioscience) and Helios-AlexaFluor647 (Biolegend) were performed using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience), according to the manufacturer’s instructions. .. For intracellular cytokine detection, CD4+ T responder cells activated for 4 days in vitro were then stimulated with 30 nM phorbol-12-myristate-13-acetate (PMA) and 200 nM ionomycin for 4 hours in the presence of GolgiStop (BD Biosciences) and intracellular staining of cytokines (IFNγ, TNFα), was performed with FOXP3 staining buffers (eBioscience) and the following antibodies: IFNγ (clone 4S.B3, eBioscience), TNFα (clone Mab11, eBioscience). .. CD19-positive cells from fetal liver, fetal bone marrow or the peripheral blood of healthy donors were enriched using the CD19 magnetic beads (Miltenyi).

    Article Title: IL-21 Therapy Controls Immune Activation and Maintains Antiviral CD8+ T Cell Responses in Acute Simian Immunodeficiency Virus Infection
    Article Snippet: After incubation, cells were washed and stained with PacBlue-conjugated anti-CD3, Qdot655-conjugated anti-CD4, and PE-Cy5.5-conjugated anti-CD8. .. Cells were then washed, permeabilized using a Cytofix/Cytoperm Kit (BD Biosciences), intracellularly stained with PE-Cy7-conjugated anti-IFNγ, Alexa 700-conjugated anti-tumor necrosis factor alpha (TNF-α), PE-conjugated anti-IL-17 (eBioscience), and FITC-conjugated anti-IL-4 (BioLegend), washed, fixed, and acquired. .. PBMCs from animals before infection were stimulated with PMA and ionomycin in the presence or absence of rIL-21-IgFc (10 ng/ml equivalent to 1 U/ml).

    Article Title: Long-lasting multifunctional CD8+ T cell responses in end-stage melanoma patients can be induced by dendritic cell vaccination
    Article Snippet: .. The cells were stained for cytokines in 50 μL PBA supplemented with 0.5% Saponin and PerCP-Cy5.5- or BV421-conjugated anti-IFNγ (B27, BD PharMingen, 1:10; 1:10), PE-labeled anti-IL-2 (MQ1–17H12, eBioscience, 1:100), FITC-labeled anti-CCL4 (24006, R & D systems, 1:5) and PE-Cy7- or PerCP-Cy5.5-labled anti-TNFα (MAb11, eBioscience, 1:100; 1:10) at 4°C for 30 min. ..

    Purification:

    Article Title: Microglial Derived Tumor Necrosis Factor-α Drives Alzheimer’s Disease-Related Neuronal Cell Cycle Events
    Article Snippet: .. For TNFα studies, prior to neuronal treatment, the AβO-activated microglial CM (with 10 µM BrdU) was mixed with purified anti-TNFα antibody (eBioscience, Cat # 14–7349–85; Clone: MAb11) or non-specific mouse IgG (Sigma-Aldrich; final concentration of 125 ng/ml) and incubated for 24 h at 37°C. .. Neurons were also treated directly with mouse IgG (125 ng/ml), recombinant TNFα (Sigma-Aldrich, Cat # T7539) or IL-6 (PeproTech, Cat # 216–16) (both at 250 pg/ml) or vehicle in the presence of BrdU (10 µM).

    Concentration Assay:

    Article Title: Microglial Derived Tumor Necrosis Factor-α Drives Alzheimer’s Disease-Related Neuronal Cell Cycle Events
    Article Snippet: .. For TNFα studies, prior to neuronal treatment, the AβO-activated microglial CM (with 10 µM BrdU) was mixed with purified anti-TNFα antibody (eBioscience, Cat # 14–7349–85; Clone: MAb11) or non-specific mouse IgG (Sigma-Aldrich; final concentration of 125 ng/ml) and incubated for 24 h at 37°C. .. Neurons were also treated directly with mouse IgG (125 ng/ml), recombinant TNFα (Sigma-Aldrich, Cat # T7539) or IL-6 (PeproTech, Cat # 216–16) (both at 250 pg/ml) or vehicle in the presence of BrdU (10 µM).

    Incubation:

    Article Title: Microglial Derived Tumor Necrosis Factor-α Drives Alzheimer’s Disease-Related Neuronal Cell Cycle Events
    Article Snippet: .. For TNFα studies, prior to neuronal treatment, the AβO-activated microglial CM (with 10 µM BrdU) was mixed with purified anti-TNFα antibody (eBioscience, Cat # 14–7349–85; Clone: MAb11) or non-specific mouse IgG (Sigma-Aldrich; final concentration of 125 ng/ml) and incubated for 24 h at 37°C. .. Neurons were also treated directly with mouse IgG (125 ng/ml), recombinant TNFα (Sigma-Aldrich, Cat # T7539) or IL-6 (PeproTech, Cat # 216–16) (both at 250 pg/ml) or vehicle in the presence of BrdU (10 µM).

    In Vitro:

    Article Title: Signaling Lymphocyte Activated Molecule (SLAM)/SLAM-associated Protein (SAP) Pathway Regulates Human B-cell Tolerance
    Article Snippet: Intracellular staining for FOXP3-AlexaFluor488 (clone PCH101, eBioscience) and Helios-AlexaFluor647 (Biolegend) were performed using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience), according to the manufacturer’s instructions. .. For intracellular cytokine detection, CD4+ T responder cells activated for 4 days in vitro were then stimulated with 30 nM phorbol-12-myristate-13-acetate (PMA) and 200 nM ionomycin for 4 hours in the presence of GolgiStop (BD Biosciences) and intracellular staining of cytokines (IFNγ, TNFα), was performed with FOXP3 staining buffers (eBioscience) and the following antibodies: IFNγ (clone 4S.B3, eBioscience), TNFα (clone Mab11, eBioscience). .. CD19-positive cells from fetal liver, fetal bone marrow or the peripheral blood of healthy donors were enriched using the CD19 magnetic beads (Miltenyi).

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  • 98
    Thermo Fisher cytokine
    <t>Cytokine</t> production by DCs infected with ES
    Cytokine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine - by Bioz Stars, 2021-06
    98/100 stars
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    99
    Thermo Fisher tnf α
    (a) Effect of QRQS on <t>TNF-</t> α – or TNF- α +IFN- γ -induced HaCaT cells to produce IL-33 mRNA and protein. TNF- α (50 ng/mL) or TNF- α (50 ng/mL) + IFN- γ (50 ng/mL) stimulated HaCaT cells were cultured with different concentrations of QRQS solution for 24 h. RT-PCR was used to detect the IL-33 mRNA secretion. (b) The influence of different time points in different concentration decoctions on the expression of the IL-33 protein. The TNF- α (50 ng/mL) + IFN- γ (50 ng/mL) combined with HaCaT cells were treated with different concentrations (0.125 g/mL, 0.5 g/mL, and 2.0 g/mL) of QRQS, and, at different time points (24, 32, 48, and 56 h), supernatants were collected. The IL-33 protein expression was detected by ELISA. The data are shown as mean ± SEMs, ### P
    Tnf α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier



    Image Search Results


    Cytokine production by DCs infected with ES

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Enterobacter sakazakii targets DC-SIGN to induce immunosuppressive responses in dendritic cells by modulating MAP kinases

    doi: 10.4049/jimmunol.0902029

    Figure Lengend Snippet: Cytokine production by DCs infected with ES

    Article Snippet: Cytokine (TNF-α, IL-1β, IL-6, IL-12 p70, IL-10 and TGF-β) production in cell culture supernatants of DC-bacteria co-culture experiments collected after 24 and 48 h of incubation was carried out using Biosource ELISA kits (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.

    Techniques: Infection

    (a) Effect of QRQS on TNF- α – or TNF- α +IFN- γ -induced HaCaT cells to produce IL-33 mRNA and protein. TNF- α (50 ng/mL) or TNF- α (50 ng/mL) + IFN- γ (50 ng/mL) stimulated HaCaT cells were cultured with different concentrations of QRQS solution for 24 h. RT-PCR was used to detect the IL-33 mRNA secretion. (b) The influence of different time points in different concentration decoctions on the expression of the IL-33 protein. The TNF- α (50 ng/mL) + IFN- γ (50 ng/mL) combined with HaCaT cells were treated with different concentrations (0.125 g/mL, 0.5 g/mL, and 2.0 g/mL) of QRQS, and, at different time points (24, 32, 48, and 56 h), supernatants were collected. The IL-33 protein expression was detected by ELISA. The data are shown as mean ± SEMs, ### P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction

    doi: 10.1155/2017/2489842

    Figure Lengend Snippet: (a) Effect of QRQS on TNF- α – or TNF- α +IFN- γ -induced HaCaT cells to produce IL-33 mRNA and protein. TNF- α (50 ng/mL) or TNF- α (50 ng/mL) + IFN- γ (50 ng/mL) stimulated HaCaT cells were cultured with different concentrations of QRQS solution for 24 h. RT-PCR was used to detect the IL-33 mRNA secretion. (b) The influence of different time points in different concentration decoctions on the expression of the IL-33 protein. The TNF- α (50 ng/mL) + IFN- γ (50 ng/mL) combined with HaCaT cells were treated with different concentrations (0.125 g/mL, 0.5 g/mL, and 2.0 g/mL) of QRQS, and, at different time points (24, 32, 48, and 56 h), supernatants were collected. The IL-33 protein expression was detected by ELISA. The data are shown as mean ± SEMs, ### P

    Article Snippet: Primers for GAPDH, IFN-γ , IL-1RAcP, TNF-α , ST2, IL-14, IL-13, and mouse and human IL-33 were obtained from Invitrogen.

    Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay

    Defective IFN-γ and anti- P . yoelii 17XNL humoral immune responses in Uba3 ΔT mice. Serum levels of proinflammatory cytokines (A) IFN-γ, (B) TNF-α, and anti-inflammatory cytokines (C) TGF-β, (D) IL-10 in Uba3 fl/fl and Uba3 ΔT mice at days 0, 3, 5, 8, 10 of P . yoelii 17XNL infection (n = 5–6 per group), as determined by ELISA. (E) Parasite-specific IgG response over the course of infection was determined by ELISA. Data are expressed as relative OD values (n = 6 per group). (F) Representative dot plots and bar graphs showing the proportions and absolute numbers of Fas + GL-7 + germinal center B cells (gated on live B220 + B cells) in spleens of Uba3 fl/fl and Uba3 ΔT mice at day 0 and day 16 p.i. (n = 5–6 per group). (G) Left, representative confocal micrographs of spleen sections from day 0 and day16 infected Uba3 fl/fl and Uba3 ΔT mice, identifying T cell zones with anti-CD3 (green), B cell follicles with anti-IgD (blue), and germinal centers (GCs) with peanut agglutinin (PNA, red) staining. Scale bars, 100 μm. Right, bar graphs showing GC numbers per two sections from the spleen of each mouse at day 16 p.i. (n = 3 per group). Data are representative of three replicate experiments and are shown as mean±SEM. * p

    Journal: PLoS Pathogens

    Article Title: Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection

    doi: 10.1371/journal.ppat.1007440

    Figure Lengend Snippet: Defective IFN-γ and anti- P . yoelii 17XNL humoral immune responses in Uba3 ΔT mice. Serum levels of proinflammatory cytokines (A) IFN-γ, (B) TNF-α, and anti-inflammatory cytokines (C) TGF-β, (D) IL-10 in Uba3 fl/fl and Uba3 ΔT mice at days 0, 3, 5, 8, 10 of P . yoelii 17XNL infection (n = 5–6 per group), as determined by ELISA. (E) Parasite-specific IgG response over the course of infection was determined by ELISA. Data are expressed as relative OD values (n = 6 per group). (F) Representative dot plots and bar graphs showing the proportions and absolute numbers of Fas + GL-7 + germinal center B cells (gated on live B220 + B cells) in spleens of Uba3 fl/fl and Uba3 ΔT mice at day 0 and day 16 p.i. (n = 5–6 per group). (G) Left, representative confocal micrographs of spleen sections from day 0 and day16 infected Uba3 fl/fl and Uba3 ΔT mice, identifying T cell zones with anti-CD3 (green), B cell follicles with anti-IgD (blue), and germinal centers (GCs) with peanut agglutinin (PNA, red) staining. Scale bars, 100 μm. Right, bar graphs showing GC numbers per two sections from the spleen of each mouse at day 16 p.i. (n = 3 per group). Data are representative of three replicate experiments and are shown as mean±SEM. * p

    Article Snippet: Cytokine and parasite-specific antibody ELISAs Levels of serum IFN-γ, TNF-α, TGF-β and IL-10 were quantified using Ready- SET-Go ELISA Kits (eBioscience).

    Techniques: Mouse Assay, Infection, Enzyme-linked Immunosorbent Assay, Staining

    Dimannoside caps are required for ManLAM binding to and signaling via Dectin-2. ManLAM (1 µg in ( A ) from 300 to 10 ng in ( B and D ) 0.1 µg in ( C )) purified from M . tuberculosis wild-type or Δ Rv2181 mutant strains were tested for their capacity to bind Dectin-2-Fc ( A ), to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and HEK-TLR2 cells ( D ), and to induce TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. NC, non-coated.

    Journal: Scientific Reports

    Article Title: Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2

    doi: 10.1038/s41598-018-35393-5

    Figure Lengend Snippet: Dimannoside caps are required for ManLAM binding to and signaling via Dectin-2. ManLAM (1 µg in ( A ) from 300 to 10 ng in ( B and D ) 0.1 µg in ( C )) purified from M . tuberculosis wild-type or Δ Rv2181 mutant strains were tested for their capacity to bind Dectin-2-Fc ( A ), to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and HEK-TLR2 cells ( D ), and to induce TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. NC, non-coated.

    Article Snippet: After 18 h, TNF-α was assayed in the culture supernatant using a commercially available kit (eBioscience).

    Techniques: Binding Assay, Purification, Mutagenesis, Activation Assay

    ManLAM, but not the other mycobacterial cell envelope mannoconjugates tested, induce cell signaling via Dectin-2. Mannoconjugates (1 µg in ( A , B ) 0.1 µg in ( C )) were coated in 96-well plates and tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs. ( A ) Dectin-2-Fc or IgG1-Fc control proteins (1 μg/ml) were pre-incubated or not with 20 mM EDTA or 40 mM mannose and allowed to react with the mannoconjugates for 2 h at RT. Bound proteins were detected using a biotin-conjugated anti-IgG Fcγ specific antibody and avidin-HRP, and reading O.D. at 450 nm. ( B ) HEK-Dectin-2 cells were stimulated for 24 h and NF-κB activation was determined by measuring alkaline phosphatase activity and reading O.D. at 630 nm. ( C ) BMDCs were stimulated for 24 h and TNF-α release in the supernatant was quantified by ELISA. Dectin-2 dependence was investigated by pre-incubating cells for 30 min at 37 °C with 5 μg/ml of anti-Dectin-2 or rIgG2a isotype control antibodies. Data show mean ± SEM. Mtb, M . tuberculosis ; Msm, M . smegmatis ; NC, non-coated; - ManLAM, ManLAM-depleted.

    Journal: Scientific Reports

    Article Title: Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2

    doi: 10.1038/s41598-018-35393-5

    Figure Lengend Snippet: ManLAM, but not the other mycobacterial cell envelope mannoconjugates tested, induce cell signaling via Dectin-2. Mannoconjugates (1 µg in ( A , B ) 0.1 µg in ( C )) were coated in 96-well plates and tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs. ( A ) Dectin-2-Fc or IgG1-Fc control proteins (1 μg/ml) were pre-incubated or not with 20 mM EDTA or 40 mM mannose and allowed to react with the mannoconjugates for 2 h at RT. Bound proteins were detected using a biotin-conjugated anti-IgG Fcγ specific antibody and avidin-HRP, and reading O.D. at 450 nm. ( B ) HEK-Dectin-2 cells were stimulated for 24 h and NF-κB activation was determined by measuring alkaline phosphatase activity and reading O.D. at 630 nm. ( C ) BMDCs were stimulated for 24 h and TNF-α release in the supernatant was quantified by ELISA. Dectin-2 dependence was investigated by pre-incubating cells for 30 min at 37 °C with 5 μg/ml of anti-Dectin-2 or rIgG2a isotype control antibodies. Data show mean ± SEM. Mtb, M . tuberculosis ; Msm, M . smegmatis ; NC, non-coated; - ManLAM, ManLAM-depleted.

    Article Snippet: After 18 h, TNF-α was assayed in the culture supernatant using a commercially available kit (eBioscience).

    Techniques: Activation Assay, Incubation, Avidin-Biotin Assay, Activity Assay, Enzyme-linked Immunosorbent Assay

    ManLAM is the sole ligand mediating M . tuberculosis recognition by Dectin-2. The indicated M . tuberculosis (Mtb) strains (10 6 bacilli in ( A ) 1 µg lysate in ( B and C )) were tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. compl., complemented; NC, non-coated.

    Journal: Scientific Reports

    Article Title: Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2

    doi: 10.1038/s41598-018-35393-5

    Figure Lengend Snippet: ManLAM is the sole ligand mediating M . tuberculosis recognition by Dectin-2. The indicated M . tuberculosis (Mtb) strains (10 6 bacilli in ( A ) 1 µg lysate in ( B and C )) were tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. compl., complemented; NC, non-coated.

    Article Snippet: After 18 h, TNF-α was assayed in the culture supernatant using a commercially available kit (eBioscience).

    Techniques: Activation Assay

    Actinobacteria lipoglycans bearing (α1 → 2)-linked dimannoside caps bind and induce signaling via Dectin-2. Lipoglycans (1 µg in ( A ) from 300 to 10 ng in ( B ) 0.1 µg in ( C )) were tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. NC, non-coated.

    Journal: Scientific Reports

    Article Title: Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2

    doi: 10.1038/s41598-018-35393-5

    Figure Lengend Snippet: Actinobacteria lipoglycans bearing (α1 → 2)-linked dimannoside caps bind and induce signaling via Dectin-2. Lipoglycans (1 µg in ( A ) from 300 to 10 ng in ( B ) 0.1 µg in ( C )) were tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. NC, non-coated.

    Article Snippet: After 18 h, TNF-α was assayed in the culture supernatant using a commercially available kit (eBioscience).

    Techniques: Activation Assay

    Mannodendrimers bind but do not induce signaling via Dectin-2. Mannoconjugates (1 µg in ( A ) from 300 to 10 ng in ( B ) 0.1 µg in ( C )) were tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. dManLAM, deacylated ManLAM; M2M, M2D, second-generation mannodendrimers capped with mono- or di-mannosides respectively; M3T, third-generation mannodendrimer capped with trimannosides; M4D, fourth-generation mannodendrimer capped with dimannosides; NC, non-coated.

    Journal: Scientific Reports

    Article Title: Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2

    doi: 10.1038/s41598-018-35393-5

    Figure Lengend Snippet: Mannodendrimers bind but do not induce signaling via Dectin-2. Mannoconjugates (1 µg in ( A ) from 300 to 10 ng in ( B ) 0.1 µg in ( C )) were tested for their capacity to bind Dectin-2-Fc ( A ), and to induce NF-κB activation in HEK-Dectin-2 cells ( B ) and TNF-α production by BMDCs ( C ). Conditions are the same as in Fig. 2 . Data show mean ± SEM. dManLAM, deacylated ManLAM; M2M, M2D, second-generation mannodendrimers capped with mono- or di-mannosides respectively; M3T, third-generation mannodendrimer capped with trimannosides; M4D, fourth-generation mannodendrimer capped with dimannosides; NC, non-coated.

    Article Snippet: After 18 h, TNF-α was assayed in the culture supernatant using a commercially available kit (eBioscience).

    Techniques: Activation Assay