tnfa  (Sino Biological)


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  • 91
    Name:
    TNF alpha TNFA TNFSF2 TNFSF2 Antibody Rabbit PAb Antigen Affinity Purified
    Description:
    Produced in rabbits immunized with purified recombinant Human TNF alpha TNFa TNFSF2 rh TNF alpha TNFa TNFSF2 Catalog 10602 HNAE NP 000585 2 Val77 Leu233 TNF alpha TNFa TNFSF2 specific IgG was purified by Human TNF alpha TNFa TNFSF2 affinity chromatography
    Catalog Number:
    10602-RP02
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    Human
    Applications:
    WB,ELISA,IP
    Immunogen:
    Recombinant Human TNF-alpha / TNFa / TNFSF2 protein (Catalog#10602-HNAE)
    Antibody Type:
    PAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological tnfa
    <t>FoxM1</t> regulates ADAM-17 expression promoting <t>TNFa</t> expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P
    Produced in rabbits immunized with purified recombinant Human TNF alpha TNFa TNFSF2 rh TNF alpha TNFa TNFSF2 Catalog 10602 HNAE NP 000585 2 Val77 Leu233 TNF alpha TNFa TNFSF2 specific IgG was purified by Human TNF alpha TNFa TNFSF2 affinity chromatography
    https://www.bioz.com/result/tnfa/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfa - by Bioz Stars, 2021-05
    91/100 stars

    Images

    1) Product Images from "ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1"

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4294-9

    FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P
    Figure Legend Snippet: FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Techniques Used: Expressing, Stable Transfection, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, shRNA, Over Expression

    2) Product Images from "ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1"

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4294-9

    FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P
    Figure Legend Snippet: FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Techniques Used: Expressing, Stable Transfection, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, shRNA, Over Expression

    Related Articles

    Western Blot:

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1
    Article Snippet: .. Western blot analysis was used to detect the protein levels of FoxM1 (K19, Santa Cruz Biotechnology), ADAM-17 (H300, Santa Cruz Biotechnology), and TNFa (10602-RP02, Sino Biological, Inc.) according to standard procedures [ – ]. .. The levels of TNFa were measured in cell-free supernatants of stably transfected FoxM1-overexpressing RBE cells, FoxM1-shRNA QBC939 cells, and the control cells.

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1
    Article Snippet: .. Western blot analysisWestern blot analysis was used to detect the protein levels of FoxM1 (K19, Santa Cruz Biotechnology), ADAM-17 (H300, Santa Cruz Biotechnology), and TNFa (10602-RP02, Sino Biological, Inc.) according to standard procedures [ – ]. .. Measurement of TNFαreleaseThe levels of TNFa were measured in cell-free supernatants of stably transfected FoxM1-overexpressing RBE cells, FoxM1-shRNA QBC939 cells, and the control cells.

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  • 94
    Sino Biological tnf α
    Upregulation of inflammatory cytokines in the infarct region of the rat heart early after myocardial infarction (MI). Representative images of immunohistochemical staining of interleukin-1β (IL-1β) (A) , interleukin-6 (IL-6) (D) , and tumor necrosis factor-α <t>(TNF-α)</t> (G) in the infarct region of the rat heart at different times after MI. Comparisons of the numbers of IL-1β + CD68 + (B) , IL-6 + CD68 + (E) , and TNF-α + CD68 + (H) cells per field, respectively, and the percentage of the surface area within the infarct region that was stained positively for IL-1β (C) , IL-6 (F) , and TNF-α (I) , respectively. In panels (A,D,G) , scale bars refer to 40 μm. **, *, and NS refer to p
    Tnf α, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α - by Bioz Stars, 2021-05
    94/100 stars
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    N/A
    This antibody was obtained from a rabbit immunized with purified recombinant Human TNF alpha TNFA TNFSF2 rh TNF alpha TNFA TNFSF2 Catalog 10602 HNAE NP 000585 2 Val77 Leu233
      Buy from Supplier

    N/A
    A DNA sequence encoding the cynomolgus TNF NP 001272206 1 Val 77 Leu233 was expressed and purified with an initial Met
      Buy from Supplier

    Image Search Results


    Upregulation of inflammatory cytokines in the infarct region of the rat heart early after myocardial infarction (MI). Representative images of immunohistochemical staining of interleukin-1β (IL-1β) (A) , interleukin-6 (IL-6) (D) , and tumor necrosis factor-α (TNF-α) (G) in the infarct region of the rat heart at different times after MI. Comparisons of the numbers of IL-1β + CD68 + (B) , IL-6 + CD68 + (E) , and TNF-α + CD68 + (H) cells per field, respectively, and the percentage of the surface area within the infarct region that was stained positively for IL-1β (C) , IL-6 (F) , and TNF-α (I) , respectively. In panels (A,D,G) , scale bars refer to 40 μm. **, *, and NS refer to p

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Inflammatory Cytokines Alter Mesenchymal Stem Cell Mechanosensing and Adhesion on Stiffened Infarct Heart Tissue After Myocardial Infarction

    doi: 10.3389/fcell.2020.583700

    Figure Lengend Snippet: Upregulation of inflammatory cytokines in the infarct region of the rat heart early after myocardial infarction (MI). Representative images of immunohistochemical staining of interleukin-1β (IL-1β) (A) , interleukin-6 (IL-6) (D) , and tumor necrosis factor-α (TNF-α) (G) in the infarct region of the rat heart at different times after MI. Comparisons of the numbers of IL-1β + CD68 + (B) , IL-6 + CD68 + (E) , and TNF-α + CD68 + (H) cells per field, respectively, and the percentage of the surface area within the infarct region that was stained positively for IL-1β (C) , IL-6 (F) , and TNF-α (I) , respectively. In panels (A,D,G) , scale bars refer to 40 μm. **, *, and NS refer to p

    Article Snippet: IL-1β, IL-6, and TNF-α were obtained from Sino Biological (Beijing, China).

    Techniques: Immunohistochemistry, Staining

    Decreased expression of FSTL1 associated with reduced IL-1β and TNF-α expression in NPC primary tumors ( A ) Human normal nasopharyngeal epithelium tissues (NNE) and NPC tumor tissues were double immunofluorescence stained with FSTL1 (green) and TNF-α (red) or IL-1β (red) antibodies. H E staining demonstrates the histomorphological characteristic of sections. Magnification, 200×. ( B ) ELISA of IL-1β and TNF-α secretion after treatment of human macrophages with soluble FSTL1 (0.75 μg/ml). Mock treatment was a control. Data are mean ± SD ( n = 3). * P

    Journal: Oncotarget

    Article Title: Epigenetic inactivation of follistatin-like 1 mediates tumor immune evasion in nasopharyngeal carcinoma

    doi: 10.18632/oncotarget.7654

    Figure Lengend Snippet: Decreased expression of FSTL1 associated with reduced IL-1β and TNF-α expression in NPC primary tumors ( A ) Human normal nasopharyngeal epithelium tissues (NNE) and NPC tumor tissues were double immunofluorescence stained with FSTL1 (green) and TNF-α (red) or IL-1β (red) antibodies. H E staining demonstrates the histomorphological characteristic of sections. Magnification, 200×. ( B ) ELISA of IL-1β and TNF-α secretion after treatment of human macrophages with soluble FSTL1 (0.75 μg/ml). Mock treatment was a control. Data are mean ± SD ( n = 3). * P

    Article Snippet: Sections were co-incubated with the antibodies for FSTL1 (1:200, Abcam, Cambridge, MA, UK) and IL-1β (1:200, Abcam) or FSTL1 and TNF-α (1:200, Abcam).

    Techniques: Expressing, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay

    Induction and inhibition of inflammation in CACO-2 cells by confocal microscopy: non-inflamed CACO2 cells ( a – d ); inflamed CACO-2 cells treated with proinflammatory cocktail comprising of IL-6, TNF-α, and LPS at 0.2, 0.3, and 20 μg/mL, respectively, for 3 h used to observe the expression of NF-κB ( e – h ). Reduction in the expression of NF-κB occured in CACO-2 cells after treatment with GAR-PLGA-ES100 NPs (250 μg/mL) for 48 h ( i – l ) (at scale 25 μm).

    Journal: Polymers

    Article Title: Garcinol Encapsulated Ph-Sensitive Biodegradable Nanoparticles: A Novel Therapeutic Strategy for the Treatment of Inflammatory Bowel Disease

    doi: 10.3390/polym13060862

    Figure Lengend Snippet: Induction and inhibition of inflammation in CACO-2 cells by confocal microscopy: non-inflamed CACO2 cells ( a – d ); inflamed CACO-2 cells treated with proinflammatory cocktail comprising of IL-6, TNF-α, and LPS at 0.2, 0.3, and 20 μg/mL, respectively, for 3 h used to observe the expression of NF-κB ( e – h ). Reduction in the expression of NF-κB occured in CACO-2 cells after treatment with GAR-PLGA-ES100 NPs (250 μg/mL) for 48 h ( i – l ) (at scale 25 μm).

    Article Snippet: TNF- α , IL-1 β and interleukin (IL)-6 proteins were bought from Sino Biological (Pennsylvania, USA).

    Techniques: Inhibition, Confocal Microscopy, Expressing

    Induction and inhibition of inflammation in CACO-2 cells by confocal microscopy: non-inflamed CACO2 cells ( a – d ); inflamed CACO-2 cells treated with proinflammatory cocktail comprising of IL-6, TNF-α, and LPS at 0.4, 0.9, and 30 μg/mL, respectively, for 3 h used to observe the expression of TNF- α ( e – h ). Reduction in the expression of TNF- α occurred in CACO-2 cells after treatment with GAR-PLGA-ES100 NPs (250 μg/mL) for 48 h ( i – l ) (at scale 25 μm).

    Journal: Polymers

    Article Title: Garcinol Encapsulated Ph-Sensitive Biodegradable Nanoparticles: A Novel Therapeutic Strategy for the Treatment of Inflammatory Bowel Disease

    doi: 10.3390/polym13060862

    Figure Lengend Snippet: Induction and inhibition of inflammation in CACO-2 cells by confocal microscopy: non-inflamed CACO2 cells ( a – d ); inflamed CACO-2 cells treated with proinflammatory cocktail comprising of IL-6, TNF-α, and LPS at 0.4, 0.9, and 30 μg/mL, respectively, for 3 h used to observe the expression of TNF- α ( e – h ). Reduction in the expression of TNF- α occurred in CACO-2 cells after treatment with GAR-PLGA-ES100 NPs (250 μg/mL) for 48 h ( i – l ) (at scale 25 μm).

    Article Snippet: TNF- α , IL-1 β and interleukin (IL)-6 proteins were bought from Sino Biological (Pennsylvania, USA).

    Techniques: Inhibition, Confocal Microscopy, Expressing

    Induction and inhibition of inflammation in CACO-2 cells by confocal microscopy: non-inflamed CACO2 cells ( a – d ); CACO-2 cells treated with proinflammatory cocktail comprising of IL-6, TNF-α, IL-1β, and LPS at 0.4, 0.9, 1, and 30 μg/mL, respectively, for 45 min demonstrated the expression of IL-8 ( e – h ). Reduction in the expression of IL-8 in CACO-2 cells was observed after treatment with GAR-PLGA-ES100 NPs (250 μg/mL) for 48 h ( i – l ) (at scale 25 μm).

    Journal: Polymers

    Article Title: Garcinol Encapsulated Ph-Sensitive Biodegradable Nanoparticles: A Novel Therapeutic Strategy for the Treatment of Inflammatory Bowel Disease

    doi: 10.3390/polym13060862

    Figure Lengend Snippet: Induction and inhibition of inflammation in CACO-2 cells by confocal microscopy: non-inflamed CACO2 cells ( a – d ); CACO-2 cells treated with proinflammatory cocktail comprising of IL-6, TNF-α, IL-1β, and LPS at 0.4, 0.9, 1, and 30 μg/mL, respectively, for 45 min demonstrated the expression of IL-8 ( e – h ). Reduction in the expression of IL-8 in CACO-2 cells was observed after treatment with GAR-PLGA-ES100 NPs (250 μg/mL) for 48 h ( i – l ) (at scale 25 μm).

    Article Snippet: TNF- α , IL-1 β and interleukin (IL)-6 proteins were bought from Sino Biological (Pennsylvania, USA).

    Techniques: Inhibition, Confocal Microscopy, Expressing