tlr9 agonist cpg dna  (Hycult Biotech)


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    Hycult Biotech tlr9 agonist cpg dna
    Tlr9 Agonist Cpg Dna, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tlr9  (Hycult Biotech)


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    Hycult Biotech tlr9
    Tlr9, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tlr9 agonist cpg dna  (Hycult Biotech)


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    Hycult Biotech tlr9 agonist cpg dna
    Tlr9 Agonist Cpg Dna, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tlr9 agonist cpg dna  (Hycult Biotech)


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    Hycult Biotech tlr9 agonist cpg dna
    Identification and distribution of <t>TLR9-expressing</t> cells in BM specimens from patients with OLP. ( A ) Representative images of immunofluorescence staining of TLR9 (red) in BM specimens from patients with OLP and HK. Scale bars, 100 μm. ( B ) Number of TLR9-positive cells in BM specimens from patients with OLP (n = 20) and HK (n = 12). ( C ) Representative images of double immunofluorescence staining for TLR9 (red), CD20 (blue), and CD123 (green) in BM specimens from patients with OLP. Scale bars, 100 μm. ( D ) Number of TLR9-expressing CD20- and CD123-positive cells in BM specimens from patients with OLP (n = 20). In ( B ) and ( D ), data are shown as box plots. Each box represents the upper and lower interquartile range. Lines inside the boxes represent the median. Symbols represent individual subjects. *** P < 0.001, **** P < 0.0001, by one-way ANOVA ( B ) or Mann–Whitney U tests ( D ). See Fig. for other definitions.
    Tlr9 Agonist Cpg Dna, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Toll-like receptor 9-positive plasmacytoid dendritic cells promote Th17 immune responses in oral lichen planus stimulated by epithelium-derived cathepsin K"

    Article Title: Toll-like receptor 9-positive plasmacytoid dendritic cells promote Th17 immune responses in oral lichen planus stimulated by epithelium-derived cathepsin K

    Journal: Scientific Reports

    doi: 10.1038/s41598-023-46090-3

    Identification and distribution of TLR9-expressing cells in BM specimens from patients with OLP. ( A ) Representative images of immunofluorescence staining of TLR9 (red) in BM specimens from patients with OLP and HK. Scale bars, 100 μm. ( B ) Number of TLR9-positive cells in BM specimens from patients with OLP (n = 20) and HK (n = 12). ( C ) Representative images of double immunofluorescence staining for TLR9 (red), CD20 (blue), and CD123 (green) in BM specimens from patients with OLP. Scale bars, 100 μm. ( D ) Number of TLR9-expressing CD20- and CD123-positive cells in BM specimens from patients with OLP (n = 20). In ( B ) and ( D ), data are shown as box plots. Each box represents the upper and lower interquartile range. Lines inside the boxes represent the median. Symbols represent individual subjects. *** P < 0.001, **** P < 0.0001, by one-way ANOVA ( B ) or Mann–Whitney U tests ( D ). See Fig. for other definitions.
    Figure Legend Snippet: Identification and distribution of TLR9-expressing cells in BM specimens from patients with OLP. ( A ) Representative images of immunofluorescence staining of TLR9 (red) in BM specimens from patients with OLP and HK. Scale bars, 100 μm. ( B ) Number of TLR9-positive cells in BM specimens from patients with OLP (n = 20) and HK (n = 12). ( C ) Representative images of double immunofluorescence staining for TLR9 (red), CD20 (blue), and CD123 (green) in BM specimens from patients with OLP. Scale bars, 100 μm. ( D ) Number of TLR9-expressing CD20- and CD123-positive cells in BM specimens from patients with OLP (n = 20). In ( B ) and ( D ), data are shown as box plots. Each box represents the upper and lower interquartile range. Lines inside the boxes represent the median. Symbols represent individual subjects. *** P < 0.001, **** P < 0.0001, by one-way ANOVA ( B ) or Mann–Whitney U tests ( D ). See Fig. for other definitions.

    Techniques Used: Expressing, Immunofluorescence, Staining, Double Immunofluorescence Staining, MANN-WHITNEY

    Association of CD123 + plasmacytoid dendritic cells (pDCs) with Th17 cell differentiation. ( A ) Schematic illustration of the experimental procedures; human CD123 + pDCs were extracted from peripheral blood mononuclear cells (PBMCs) and then stimulated by the Toll-like receptor 9 (TLR9) agonist CpG DNA, TLR9 inhibitor iODN, and/or CTSK in vitro. ( B ) Production of Th17-related cytokines (IL-6, IL-23, and TGF-β) in CD123 + pDCs stimulated with CpG DNA, iODN, and/or CTSK (n = 3/sample). Bars show the mean ± SD. Symbols represent individual subjects. * P < 0.05, ** P < 0.01, by one-way ANOVA. ( C ) Representative images of immunofluorescence staining for CD4 (green), RORγt (pink) and DAPI (blue) in BM specimens from patients with OLP and HK. Scale bars, 100 μm. ( D ), ( E ) Quantification of CD4 + RORγt + Th17 cells in BM specimens from patients with OLP (n = 20) and HK (n = 12). ( F ) Correlation among the numbers of CTSK + cells, TLR9 + CD123 + pDCs, and CD4 + RORγt + Th17 cells in BM specimens from patients with OLP (n = 20). The correlation coefficients were determined by Spearman’s rank correlations. ( G ) Number of TLR9 + CD123 + pDCs and CD4 + RORγt + Th17 cells in BM specimens from patients with reticular type OLP (n = 9) and erosive type OLP (n = 11). In (D ), ( E ) and ( G ) data are shown as box plots. Each box represents the upper and lower interquartile range. Lines inside the boxes represent the median. Symbols represent individual subjects. ** P < 0.01, *** P < 0.001, **** P < 0.0001 by one-way ANOVA ( D and E ) or Mann–Whitney U tests ( G ). See Fig. for other definitions.
    Figure Legend Snippet: Association of CD123 + plasmacytoid dendritic cells (pDCs) with Th17 cell differentiation. ( A ) Schematic illustration of the experimental procedures; human CD123 + pDCs were extracted from peripheral blood mononuclear cells (PBMCs) and then stimulated by the Toll-like receptor 9 (TLR9) agonist CpG DNA, TLR9 inhibitor iODN, and/or CTSK in vitro. ( B ) Production of Th17-related cytokines (IL-6, IL-23, and TGF-β) in CD123 + pDCs stimulated with CpG DNA, iODN, and/or CTSK (n = 3/sample). Bars show the mean ± SD. Symbols represent individual subjects. * P < 0.05, ** P < 0.01, by one-way ANOVA. ( C ) Representative images of immunofluorescence staining for CD4 (green), RORγt (pink) and DAPI (blue) in BM specimens from patients with OLP and HK. Scale bars, 100 μm. ( D ), ( E ) Quantification of CD4 + RORγt + Th17 cells in BM specimens from patients with OLP (n = 20) and HK (n = 12). ( F ) Correlation among the numbers of CTSK + cells, TLR9 + CD123 + pDCs, and CD4 + RORγt + Th17 cells in BM specimens from patients with OLP (n = 20). The correlation coefficients were determined by Spearman’s rank correlations. ( G ) Number of TLR9 + CD123 + pDCs and CD4 + RORγt + Th17 cells in BM specimens from patients with reticular type OLP (n = 9) and erosive type OLP (n = 11). In (D ), ( E ) and ( G ) data are shown as box plots. Each box represents the upper and lower interquartile range. Lines inside the boxes represent the median. Symbols represent individual subjects. ** P < 0.01, *** P < 0.001, **** P < 0.0001 by one-way ANOVA ( D and E ) or Mann–Whitney U tests ( G ). See Fig. for other definitions.

    Techniques Used: Cell Differentiation, In Vitro, Immunofluorescence, Staining, MANN-WHITNEY

    Single cell RNA-sequencing (scRNA-seq) analysis in BM specimens from patients with OLP. ( A ) Uniform manifold approximation and projection (UMAP) of CD45 + immune cells colored by cell types. ( B ) UMAP colored by TLR9 expression. ( C ) The violin plots showing the expression pattern of the selected cell markers in each of the clusters. ( D ) The percentage of CD45 + immune cells in TLR9 + cells. ( E ) KEGG pathways of upregulated DEGs in TLR9 + pDC compared with TLR9 − pDC. ( F ) GO analysis of DEGs upregulated in TLR9 + pDC compared with TLR9 − pDC.
    Figure Legend Snippet: Single cell RNA-sequencing (scRNA-seq) analysis in BM specimens from patients with OLP. ( A ) Uniform manifold approximation and projection (UMAP) of CD45 + immune cells colored by cell types. ( B ) UMAP colored by TLR9 expression. ( C ) The violin plots showing the expression pattern of the selected cell markers in each of the clusters. ( D ) The percentage of CD45 + immune cells in TLR9 + cells. ( E ) KEGG pathways of upregulated DEGs in TLR9 + pDC compared with TLR9 − pDC. ( F ) GO analysis of DEGs upregulated in TLR9 + pDC compared with TLR9 − pDC.

    Techniques Used: RNA Sequencing Assay, Expressing

    Schematic model of CTSK-induced TLR9 signaling in pDCs, leading to inflammation in OLP. TLR9 expressed on CD123-positive pDCs in subepithelial layers recognizes viral DNA. CTSK released from the LE activates TLR9 signaling in pDCs and their production of Th17-related cytokines, including IL-6, IL-23, and TGFβ, which leads to Th17-associated inflammation in OLP. See Fig. for other definitions.
    Figure Legend Snippet: Schematic model of CTSK-induced TLR9 signaling in pDCs, leading to inflammation in OLP. TLR9 expressed on CD123-positive pDCs in subepithelial layers recognizes viral DNA. CTSK released from the LE activates TLR9 signaling in pDCs and their production of Th17-related cytokines, including IL-6, IL-23, and TGFβ, which leads to Th17-associated inflammation in OLP. See Fig. for other definitions.

    Techniques Used:

    tlr9 agonist cpg dna  (Hycult Biotech)


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    Hycult Biotech tlr9 agonist cpg dna
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    biotinylated anti mouse tlr9  (Hycult Biotech)


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    Hycult Biotech biotinylated anti mouse tlr9
    Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , <t>TLR9,</t> MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.
    Biotinylated Anti Mouse Tlr9, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Pre-existing Schistosoma japonicum infection alters the immune response to Plasmodium berghei infection in C57BL/6 mice"

    Article Title: Pre-existing Schistosoma japonicum infection alters the immune response to Plasmodium berghei infection in C57BL/6 mice

    Journal: Malaria Journal

    doi: 10.1186/1475-2875-12-322

    Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , TLR9, MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.
    Figure Legend Snippet: Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , TLR9, MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.

    Techniques Used: Infection, Flow Cytometry, Expressing

    tlr9 agonist cpg b odn2006  (Hycult Biotech)


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    Hycult Biotech tlr9 agonist cpg b odn2006
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    tlr9  (Hycult Biotech)


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    Hycult Biotech tlr9
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    tlr9 stimulation  (Hycult Biotech)


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    tlr9 agonist cpg b odn2006  (Hycult Biotech)


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    Hycult Biotech tlr9 agonist cpg b odn2006
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    tlr9 agonist cpg b odn2006  (Hycult Biotech)


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    Hycult Biotech tlr9 agonist cpg dna
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    Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , <t>TLR9,</t> MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.
    Biotinylated Anti Mouse Tlr9, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech tlr9 agonist cpg b odn2006
    Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , <t>TLR9,</t> MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.
    Tlr9 Agonist Cpg B Odn2006, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech tlr9 stimulation
    Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , <t>TLR9,</t> MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.
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    Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , TLR9, MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.

    Journal: Malaria Journal

    Article Title: Pre-existing Schistosoma japonicum infection alters the immune response to Plasmodium berghei infection in C57BL/6 mice

    doi: 10.1186/1475-2875-12-322

    Figure Lengend Snippet: Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , TLR9, MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.

    Article Snippet: After fixation and permeabilization using staining buffer reagents as instructed by the manufacturer, cells were incubated with biotinylated anti-mouse TLR9 (clone 5G5, Hycult Biotechnology (HBT), Uden, The Netherlands) followed by PE-conjugated streptavidin (Biolegend, San Diego, CA, USA).

    Techniques: Infection, Flow Cytometry, Expressing