tlr4  (Novus Biologicals)


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    Structured Review

    Novus Biologicals tlr4
    Deletion of <t>Tlr4</t> suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tlr4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr4/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr4 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Immunohistochemical staining, Expressing, Western Blot

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation
    Figure Legend Snippet: The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation

    Techniques Used: Activation Assay

    tlr4  (Novus Biologicals)


    Bioz Verified Symbol Novus Biologicals is a verified supplier
    Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
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  • Team
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  • Contact
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  • Bioz vStars
  • 94

    Structured Review

    Novus Biologicals tlr4
    Deletion of <t>Tlr4</t> suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tlr4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr4/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr4 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Immunohistochemical staining, Expressing, Western Blot

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation
    Figure Legend Snippet: The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation

    Techniques Used: Activation Assay

    tlr4  (Novus Biologicals)


    Bioz Verified Symbol Novus Biologicals is a verified supplier
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    Structured Review

    Novus Biologicals tlr4
    Deletion of <t>Tlr4</t> suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tlr4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr4/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr4 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Immunohistochemical staining, Expressing, Western Blot

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation
    Figure Legend Snippet: The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation

    Techniques Used: Activation Assay

    tlr4  (Novus Biologicals)


    Bioz Verified Symbol Novus Biologicals is a verified supplier
    Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
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  • 94

    Structured Review

    Novus Biologicals tlr4
    Deletion of <t>Tlr4</t> suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tlr4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr4/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr4 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Immunohistochemical staining, Expressing, Western Blot

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation
    Figure Legend Snippet: The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation

    Techniques Used: Activation Assay

    tlr4  (Novus Biologicals)


    Bioz Verified Symbol Novus Biologicals is a verified supplier
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    Structured Review

    Novus Biologicals tlr4
    Deletion of <t>Tlr4</t> suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tlr4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
    tlr4 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Immunohistochemical staining, Expressing, Western Blot

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation
    Figure Legend Snippet: The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation

    Techniques Used: Activation Assay

    antibodies tlr4  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc antibodies tlr4
    Electroacupuncture alters the activation of <t>TLR4</t> and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.
    Antibodies Tlr4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies tlr4/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies tlr4 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia"

    Article Title: Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

    Journal: Neuroreport

    doi: 10.1097/WNR.0000000000002067

    Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.
    Figure Legend Snippet: Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.

    Techniques Used: Activation Assay, Immunofluorescence

    Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.
    Figure Legend Snippet: Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.

    Techniques Used: Expressing

    primary antibody tlr4  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc primary antibody tlr4
    Electroacupuncture alters the activation of <t>TLR4</t> and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.
    Primary Antibody Tlr4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibody tlr4/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    Images

    1) Product Images from "Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia"

    Article Title: Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

    Journal: Neuroreport

    doi: 10.1097/WNR.0000000000002067

    Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.
    Figure Legend Snippet: Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.

    Techniques Used: Activation Assay, Immunofluorescence

    Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.
    Figure Legend Snippet: Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.

    Techniques Used: Expressing


    Structured Review

    Millipore tak 5 µm tlr4 inhibitor
    Effect of TLR2 and <t>TLR4</t> on CF-mediated activation of macrophages in RAW264.7 cells. RAW264.7 cells were pretreated with C29 (TLR2 inhibitor, 100 µM) or <t>TAK</t> (TLR4 inhibitor, 5 µM) for 2 h and then co-treated with CF (200 µg/ml) for 24 h. (A) The level of NO, (B) reverse transcription PCR analysis of iNOS, IL-1β and TNF-α, and (C) phagocytotic activity in RAW264.7 cells treated with CF for 24 h in the presence of C29 or TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. TLR, toll-like receptor; CF, Chrysosplenium flagelliferum ; TAK, TAK-242; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; CON, control; DM, DMSO group.
    Tak 5 µm Tlr4 Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Anti‑obesity and immunostimulatory activity of Chrysosplenium flagelliferum in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells"

    Article Title: Anti‑obesity and immunostimulatory activity of Chrysosplenium flagelliferum in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2024.12604

    Effect of TLR2 and TLR4 on CF-mediated activation of macrophages in RAW264.7 cells. RAW264.7 cells were pretreated with C29 (TLR2 inhibitor, 100 µM) or TAK (TLR4 inhibitor, 5 µM) for 2 h and then co-treated with CF (200 µg/ml) for 24 h. (A) The level of NO, (B) reverse transcription PCR analysis of iNOS, IL-1β and TNF-α, and (C) phagocytotic activity in RAW264.7 cells treated with CF for 24 h in the presence of C29 or TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. TLR, toll-like receptor; CF, Chrysosplenium flagelliferum ; TAK, TAK-242; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; CON, control; DM, DMSO group.
    Figure Legend Snippet: Effect of TLR2 and TLR4 on CF-mediated activation of macrophages in RAW264.7 cells. RAW264.7 cells were pretreated with C29 (TLR2 inhibitor, 100 µM) or TAK (TLR4 inhibitor, 5 µM) for 2 h and then co-treated with CF (200 µg/ml) for 24 h. (A) The level of NO, (B) reverse transcription PCR analysis of iNOS, IL-1β and TNF-α, and (C) phagocytotic activity in RAW264.7 cells treated with CF for 24 h in the presence of C29 or TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. TLR, toll-like receptor; CF, Chrysosplenium flagelliferum ; TAK, TAK-242; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; CON, control; DM, DMSO group.

    Techniques Used: Activation Assay, Reverse Transcription, Activity Assay, Control

    Effect of MAPK and NF-κB signaling pathways on CF-mediated activation of macrophages in RAW264.7 cells. (A) RAW264.7 cells were pretreated with PD (ERK1/2 inhibitor, 20 µM), SB (p38 inhibitor, 20 µM) or SP (JNK inhibitor, 20 µM) and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and (B) RT-PCR analysis of iNOS, IL-1β and TNF-α in RAW264.7 cells treated with CF in the presence of PD, SB or SP. (C) RAW264.7 cells were pretreated with BAY (NF-κB inhibitor, 10 µM), and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and RT-PCR analysis of iNOS, IL-1β, and TNF-α in RAW264.7 cells treated with CF for 24 h in the presence of BAY. (D) RAW264.7 cells were treated with CF (200 µM) for the indicated time-points. Western blot analysis of p-JNK, JNK, p-p65 and p65 in RAW264.7 cells treated with CF. (E) RAW264.7 cells were pretreated with TAK (TLR4 inhibitor, 5 µM) for 2 h and the co-treated with CF (200 µM) for 3 h. Western blot analysis of p-JNK, JNK, p-p65, and p65 in RAW264.7 cells treated with CF in the presence of TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. CF, Chrysosplenium flagelliferum ; PD, PD98059; SB, SB203580; SP, SP600125; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; BAY, BAY11-7082; p-, phosphorylated; JNK, c-Jun N-terminal kinase; TAK, TAK-242; CON, control; DM, DMSO group.
    Figure Legend Snippet: Effect of MAPK and NF-κB signaling pathways on CF-mediated activation of macrophages in RAW264.7 cells. (A) RAW264.7 cells were pretreated with PD (ERK1/2 inhibitor, 20 µM), SB (p38 inhibitor, 20 µM) or SP (JNK inhibitor, 20 µM) and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and (B) RT-PCR analysis of iNOS, IL-1β and TNF-α in RAW264.7 cells treated with CF in the presence of PD, SB or SP. (C) RAW264.7 cells were pretreated with BAY (NF-κB inhibitor, 10 µM), and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and RT-PCR analysis of iNOS, IL-1β, and TNF-α in RAW264.7 cells treated with CF for 24 h in the presence of BAY. (D) RAW264.7 cells were treated with CF (200 µM) for the indicated time-points. Western blot analysis of p-JNK, JNK, p-p65 and p65 in RAW264.7 cells treated with CF. (E) RAW264.7 cells were pretreated with TAK (TLR4 inhibitor, 5 µM) for 2 h and the co-treated with CF (200 µM) for 3 h. Western blot analysis of p-JNK, JNK, p-p65, and p65 in RAW264.7 cells treated with CF in the presence of TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. CF, Chrysosplenium flagelliferum ; PD, PD98059; SB, SB203580; SP, SP600125; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; BAY, BAY11-7082; p-, phosphorylated; JNK, c-Jun N-terminal kinase; TAK, TAK-242; CON, control; DM, DMSO group.

    Techniques Used: Activation Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

    tlr4 apc  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Thermo Fisher tlr4 apc
    a Histograms and bar graphs showing percentages of CD38+ and Egr2+ BMDM among all BMDM after M1- or M2-like activation, respectively (n = 3 biological replicates). Independent experiments in Supplementary Fig. . b Immunoblot analysis of WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . c Histograms and bar graph showing percentages of MyD88-expressing BMDM after PBS or LPS stimulation (n = 3 biological replicates). Results are pooled from 3 independent experiments with each 1 pair of mice; each point represents results from individual experiment. Independent experiment in Supplementary Fig. . d Confocal fluorescence microscopy images, showing MyD88 and <t>TLR4</t> in WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . e Illustration of the experimental design in ( f – i ). Myris, myristoylation f Confocal fluorescence microcopy showing MyD88 and TLR4 in PBS-/LPS-treated WT or Lyz2 -cre BMDM transduced with MigR1-GFP or MigR1-GFP-MyrisMyD88. Cell sizes were visualized in bar graphs (WT MigR1-GFP: n = 4; Lyz2 -cre MigR1-GFP: n = 6; MigR1-GFP-MyrisMyD88: n = 3 biological replicates). Bar graph data are pooled from 3 individual experiments (more images in Supplementary Fig. ). g Representative flow cytometry dot plots, showing transduction efficiency in LPS-activated WT or Lyz2 -cre BMDM. More data in Supplementary Fig. . h Flow cytometry dot plots and bar graphs, showing percentages of FSC-A high , SSC-A high GFP + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . i Dot plots and bar graphs showing percentages of GFP + CD38 + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . Data are presented as mean ± SD ( a , c , f , h , i ). Statistical comparisons were performed with two-tailed unpaired Student’s t -tests ( a , f ; data points were normally distributed) and one-way ANOVA tests ( c , h , i ; for simultaneous comparisons of more than two groups). 9-week-old female and male ( a – d , f – i ) C57BL/6 mice were used. e Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a file.
    Tlr4 Apc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Sphinganine recruits TLR4 adaptors in macrophages and promotes inflammation in murine models of sepsis and melanoma"

    Article Title: Sphinganine recruits TLR4 adaptors in macrophages and promotes inflammation in murine models of sepsis and melanoma

    Journal: Nature Communications

    doi: 10.1038/s41467-024-50341-w

    a Histograms and bar graphs showing percentages of CD38+ and Egr2+ BMDM among all BMDM after M1- or M2-like activation, respectively (n = 3 biological replicates). Independent experiments in Supplementary Fig. . b Immunoblot analysis of WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . c Histograms and bar graph showing percentages of MyD88-expressing BMDM after PBS or LPS stimulation (n = 3 biological replicates). Results are pooled from 3 independent experiments with each 1 pair of mice; each point represents results from individual experiment. Independent experiment in Supplementary Fig. . d Confocal fluorescence microscopy images, showing MyD88 and TLR4 in WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . e Illustration of the experimental design in ( f – i ). Myris, myristoylation f Confocal fluorescence microcopy showing MyD88 and TLR4 in PBS-/LPS-treated WT or Lyz2 -cre BMDM transduced with MigR1-GFP or MigR1-GFP-MyrisMyD88. Cell sizes were visualized in bar graphs (WT MigR1-GFP: n = 4; Lyz2 -cre MigR1-GFP: n = 6; MigR1-GFP-MyrisMyD88: n = 3 biological replicates). Bar graph data are pooled from 3 individual experiments (more images in Supplementary Fig. ). g Representative flow cytometry dot plots, showing transduction efficiency in LPS-activated WT or Lyz2 -cre BMDM. More data in Supplementary Fig. . h Flow cytometry dot plots and bar graphs, showing percentages of FSC-A high , SSC-A high GFP + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . i Dot plots and bar graphs showing percentages of GFP + CD38 + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . Data are presented as mean ± SD ( a , c , f , h , i ). Statistical comparisons were performed with two-tailed unpaired Student’s t -tests ( a , f ; data points were normally distributed) and one-way ANOVA tests ( c , h , i ; for simultaneous comparisons of more than two groups). 9-week-old female and male ( a – d , f – i ) C57BL/6 mice were used. e Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a file.
    Figure Legend Snippet: a Histograms and bar graphs showing percentages of CD38+ and Egr2+ BMDM among all BMDM after M1- or M2-like activation, respectively (n = 3 biological replicates). Independent experiments in Supplementary Fig. . b Immunoblot analysis of WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . c Histograms and bar graph showing percentages of MyD88-expressing BMDM after PBS or LPS stimulation (n = 3 biological replicates). Results are pooled from 3 independent experiments with each 1 pair of mice; each point represents results from individual experiment. Independent experiment in Supplementary Fig. . d Confocal fluorescence microscopy images, showing MyD88 and TLR4 in WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . e Illustration of the experimental design in ( f – i ). Myris, myristoylation f Confocal fluorescence microcopy showing MyD88 and TLR4 in PBS-/LPS-treated WT or Lyz2 -cre BMDM transduced with MigR1-GFP or MigR1-GFP-MyrisMyD88. Cell sizes were visualized in bar graphs (WT MigR1-GFP: n = 4; Lyz2 -cre MigR1-GFP: n = 6; MigR1-GFP-MyrisMyD88: n = 3 biological replicates). Bar graph data are pooled from 3 individual experiments (more images in Supplementary Fig. ). g Representative flow cytometry dot plots, showing transduction efficiency in LPS-activated WT or Lyz2 -cre BMDM. More data in Supplementary Fig. . h Flow cytometry dot plots and bar graphs, showing percentages of FSC-A high , SSC-A high GFP + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . i Dot plots and bar graphs showing percentages of GFP + CD38 + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . Data are presented as mean ± SD ( a , c , f , h , i ). Statistical comparisons were performed with two-tailed unpaired Student’s t -tests ( a , f ; data points were normally distributed) and one-way ANOVA tests ( c , h , i ; for simultaneous comparisons of more than two groups). 9-week-old female and male ( a – d , f – i ) C57BL/6 mice were used. e Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a file.

    Techniques Used: Activation Assay, Western Blot, Expressing, Fluorescence, Microscopy, Transduction, Flow Cytometry, Two Tailed Test

    Illustration of the role of Sptlc2-derived sphinganine onto LPS-induced TLR4 signaling in macrophages. LPS induces Sptlc2-mediated sphinganine production and sphinganine interacts physically with TIRAP and MyD88 to recruit MyD88 to TLR4 in the macrophage membrane. This induces downstream signaling and results in M1-like activation-associated morphologic changes and cytokine release, mediating a strengthened acute inflammatory response. In absence of Sptlc2, MyD88 is not recruited to the membrane, preventing activation of downstream signaling. Figure 6 created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
    Figure Legend Snippet: Illustration of the role of Sptlc2-derived sphinganine onto LPS-induced TLR4 signaling in macrophages. LPS induces Sptlc2-mediated sphinganine production and sphinganine interacts physically with TIRAP and MyD88 to recruit MyD88 to TLR4 in the macrophage membrane. This induces downstream signaling and results in M1-like activation-associated morphologic changes and cytokine release, mediating a strengthened acute inflammatory response. In absence of Sptlc2, MyD88 is not recruited to the membrane, preventing activation of downstream signaling. Figure 6 created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

    Techniques Used: Derivative Assay, Membrane, Activation Assay

    tlr4 rs1927914  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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    New England Biolabs tlr4 rs1927914
    Genotype frequencies of toll-like receptor family gene and their association with Colon cancer
    Tlr4 Rs1927914, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk"

    Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk

    Journal: BMC Cancer

    doi: 10.1186/s12885-024-12604-z

    Genotype frequencies of toll-like receptor family gene and their association with Colon cancer
    Figure Legend Snippet: Genotype frequencies of toll-like receptor family gene and their association with Colon cancer

    Techniques Used:

    Stratified analysis between genotypes of  TLR4 rs1927914  and colon cancer risk
    Figure Legend Snippet: Stratified analysis between genotypes of TLR4 rs1927914 and colon cancer risk

    Techniques Used:

    The functional analysis of TLR4 rs1927914 polymorphism. ( A ) Luciferase expression of two constructers (pGL3-rs1927914G and pGL3-rs1927914A) in HCT116 and LOVO cells co-transfected with pRL-SV40 to standardize transfection efficiency. Fold increase was measured by setting the activity of the empty pGL3-Basic vector as 1. * P < 0.05 ** P < 0.01 compared with each of the construct counterparts. ( B ) Electrophoretic mobility shift assays with biotin-labeled oligonucleotide probes containing TLR4 rs1927914 A or G allele. Nuclear extracts were incubated with 5’-Biotin-TCTAGGACTTAGCAT A CAAATATTCCTGTT-3’ (A probe, lanes 1–3) or 5’-Biotin-TCTAGGACTTAGCAT G CAAATATTCCTGTT (G probe, lanes 4–6). Lanes 1 and 4 show the gel mobilities of the biotin-labeled probes without nuclear extracts; lanes 2 and 5 show the mobilities of the biotin-labeled probes with nuclear extracts in the absence of unlabeled probes. The binding specificity was confirmed by competing the biotin-labeled A or G probe with a 100-fold molar excess of unlabeled A (lane 3) or G probe (lane 6). ( C ) Transcription factor prediction using JASPAR and AliBaba showed that rs1927914 was consistent with the binding sequence of Oct-1
    Figure Legend Snippet: The functional analysis of TLR4 rs1927914 polymorphism. ( A ) Luciferase expression of two constructers (pGL3-rs1927914G and pGL3-rs1927914A) in HCT116 and LOVO cells co-transfected with pRL-SV40 to standardize transfection efficiency. Fold increase was measured by setting the activity of the empty pGL3-Basic vector as 1. * P < 0.05 ** P < 0.01 compared with each of the construct counterparts. ( B ) Electrophoretic mobility shift assays with biotin-labeled oligonucleotide probes containing TLR4 rs1927914 A or G allele. Nuclear extracts were incubated with 5’-Biotin-TCTAGGACTTAGCAT A CAAATATTCCTGTT-3’ (A probe, lanes 1–3) or 5’-Biotin-TCTAGGACTTAGCAT G CAAATATTCCTGTT (G probe, lanes 4–6). Lanes 1 and 4 show the gel mobilities of the biotin-labeled probes without nuclear extracts; lanes 2 and 5 show the mobilities of the biotin-labeled probes with nuclear extracts in the absence of unlabeled probes. The binding specificity was confirmed by competing the biotin-labeled A or G probe with a 100-fold molar excess of unlabeled A (lane 3) or G probe (lane 6). ( C ) Transcription factor prediction using JASPAR and AliBaba showed that rs1927914 was consistent with the binding sequence of Oct-1

    Techniques Used: Functional Assay, Luciferase, Expressing, Transfection, Activity Assay, Plasmid Preparation, Construct, Electrophoretic Mobility Shift Assay, Labeling, Incubation, Binding Assay, Sequencing


    Structured Review

    BIOTEC Co Ltd tlr4
    Tlr4, supplied by BIOTEC Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    tlr4 - by Bioz Stars, 2024-07
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    Novus Biologicals tlr4
    Deletion of <t>Tlr4</t> suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tlr4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Cell Signaling Technology Inc antibodies tlr4
    Electroacupuncture alters the activation of <t>TLR4</t> and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.
    Antibodies Tlr4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc primary antibody tlr4
    Electroacupuncture alters the activation of <t>TLR4</t> and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.
    Primary Antibody Tlr4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tak 5 µm tlr4 inhibitor
    Effect of TLR2 and <t>TLR4</t> on CF-mediated activation of macrophages in RAW264.7 cells. RAW264.7 cells were pretreated with C29 (TLR2 inhibitor, 100 µM) or <t>TAK</t> (TLR4 inhibitor, 5 µM) for 2 h and then co-treated with CF (200 µg/ml) for 24 h. (A) The level of NO, (B) reverse transcription PCR analysis of iNOS, IL-1β and TNF-α, and (C) phagocytotic activity in RAW264.7 cells treated with CF for 24 h in the presence of C29 or TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. TLR, toll-like receptor; CF, Chrysosplenium flagelliferum ; TAK, TAK-242; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; CON, control; DM, DMSO group.
    Tak 5 µm Tlr4 Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tlr4 apc
    a Histograms and bar graphs showing percentages of CD38+ and Egr2+ BMDM among all BMDM after M1- or M2-like activation, respectively (n = 3 biological replicates). Independent experiments in Supplementary Fig. . b Immunoblot analysis of WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . c Histograms and bar graph showing percentages of MyD88-expressing BMDM after PBS or LPS stimulation (n = 3 biological replicates). Results are pooled from 3 independent experiments with each 1 pair of mice; each point represents results from individual experiment. Independent experiment in Supplementary Fig. . d Confocal fluorescence microscopy images, showing MyD88 and <t>TLR4</t> in WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . e Illustration of the experimental design in ( f – i ). Myris, myristoylation f Confocal fluorescence microcopy showing MyD88 and TLR4 in PBS-/LPS-treated WT or Lyz2 -cre BMDM transduced with MigR1-GFP or MigR1-GFP-MyrisMyD88. Cell sizes were visualized in bar graphs (WT MigR1-GFP: n = 4; Lyz2 -cre MigR1-GFP: n = 6; MigR1-GFP-MyrisMyD88: n = 3 biological replicates). Bar graph data are pooled from 3 individual experiments (more images in Supplementary Fig. ). g Representative flow cytometry dot plots, showing transduction efficiency in LPS-activated WT or Lyz2 -cre BMDM. More data in Supplementary Fig. . h Flow cytometry dot plots and bar graphs, showing percentages of FSC-A high , SSC-A high GFP + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . i Dot plots and bar graphs showing percentages of GFP + CD38 + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . Data are presented as mean ± SD ( a , c , f , h , i ). Statistical comparisons were performed with two-tailed unpaired Student’s t -tests ( a , f ; data points were normally distributed) and one-way ANOVA tests ( c , h , i ; for simultaneous comparisons of more than two groups). 9-week-old female and male ( a – d , f – i ) C57BL/6 mice were used. e Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a file.
    Tlr4 Apc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs tlr4 rs1927914
    Genotype frequencies of toll-like receptor family gene and their association with Colon cancer
    Tlr4 Rs1927914, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BIOTEC Co Ltd tlr4
    Genotype frequencies of toll-like receptor family gene and their association with Colon cancer
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    Image Search Results


    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Article Snippet: The membranes were sealed in 5% BSA solution at room temperature for 1 h and incubated with antibodies to α-SMA (1:2000, ab124964, Abcam), Ctgf (1:2000, ab209780, Abcam), Collagen I (1:2000, ab260043, Abcam), Tlr4 (1:1000, NBP2-24821, Novus Biological), Nlrp3 (1:1000, MAB7578-SP, Novus Biological), GAPDH (1:5000, AF1186, Beyotime) overnight at 4 °C.

    Techniques: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: SP inhibits the ConA-induced Tlr4/Nlrp3 axis in the liver by reducing Veillonella. A The intersection of LC-relate genes in GeneCards and genes manipulated by Parabacteroides, Bacteroides, and Veillonella. B The PPI network was established using the 59 intersecting candidates. C, D Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 0.8831 (5, 42), p = 0.5008) and Nlrp3 (F (DFn, DFd) = 5.132 (5, 42), p = 0.0009) expression in mouse liver tissues. E The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) and Nlrp3 (F (DFn, DFd) = 2.116 (2, 2), p = 0.6418) protein expression in HSC using western blot. F The effect of Veillonella-CM treatment on Tlr4 (F (DFn, DFd) = 1.105 (2, 2), p = 0.9500) and Nlrp3 (F (DFn, DFd) = 7.000 (2, 2), p = 0.2500) protein expression in HC using western blot. Data are expressed using dots and boxplots (n = 3 or 8). F test [F (DFn, DFd)] was used to test the equal variances between two sets of data, and data satisfying equal variances were analyzed by unpaired t-test (E, F). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances between multiple data sets. Data satisfying equal variances were analyzed by one-way ANOVA test (C), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (D), and Games-Howell's multiple comparisons test was used for post hoc testing. **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: The membranes were sealed in 5% BSA solution at room temperature for 1 h and incubated with antibodies to α-SMA (1:2000, ab124964, Abcam), Ctgf (1:2000, ab209780, Abcam), Collagen I (1:2000, ab260043, Abcam), Tlr4 (1:1000, NBP2-24821, Novus Biological), Nlrp3 (1:1000, MAB7578-SP, Novus Biological), GAPDH (1:5000, AF1186, Beyotime) overnight at 4 °C.

    Techniques: Immunohistochemical staining, Expressing, Western Blot

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: The membranes were sealed in 5% BSA solution at room temperature for 1 h and incubated with antibodies to α-SMA (1:2000, ab124964, Abcam), Ctgf (1:2000, ab209780, Abcam), Collagen I (1:2000, ab260043, Abcam), Tlr4 (1:1000, NBP2-24821, Novus Biological), Nlrp3 (1:1000, MAB7578-SP, Novus Biological), GAPDH (1:5000, AF1186, Beyotime) overnight at 4 °C.

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: The diagram. SP inhibits LC progression by restoring the gut microbiota balance and reducing Tlr4/Nlrp3 signaling, thereby preventing HC pyroptosis and HSC activation

    Article Snippet: The membranes were sealed in 5% BSA solution at room temperature for 1 h and incubated with antibodies to α-SMA (1:2000, ab124964, Abcam), Ctgf (1:2000, ab209780, Abcam), Collagen I (1:2000, ab260043, Abcam), Tlr4 (1:1000, NBP2-24821, Novus Biological), Nlrp3 (1:1000, MAB7578-SP, Novus Biological), GAPDH (1:5000, AF1186, Beyotime) overnight at 4 °C.

    Techniques: Activation Assay

    Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.

    Journal: Neuroreport

    Article Title: Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

    doi: 10.1097/WNR.0000000000002067

    Figure Lengend Snippet: Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.

    Article Snippet: Next, the protein strip was charged to the PVDF membrane (Bio-Rad, DDY-7B III), blocked with 5% skimmed milk powder at room temperature for 1.5 h, and incubated with primary antibodies TLR4 (1 : 1000; Cell Signaling Technology, #13674S), MyD88 (1 : 1000; Cell Signaling Technology, #3699S), NF-κB (1 : 1000; Cell Signaling Technology, #8242S), NLRP3 (1 : 1000; Abcam, ab270449), GAPDH (1 : 1000; Cell Signaling Technology, #2118S) at 4°C overnight.

    Techniques: Activation Assay, Immunofluorescence

    Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.

    Journal: Neuroreport

    Article Title: Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

    doi: 10.1097/WNR.0000000000002067

    Figure Lengend Snippet: Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.

    Article Snippet: Next, the protein strip was charged to the PVDF membrane (Bio-Rad, DDY-7B III), blocked with 5% skimmed milk powder at room temperature for 1.5 h, and incubated with primary antibodies TLR4 (1 : 1000; Cell Signaling Technology, #13674S), MyD88 (1 : 1000; Cell Signaling Technology, #3699S), NF-κB (1 : 1000; Cell Signaling Technology, #8242S), NLRP3 (1 : 1000; Abcam, ab270449), GAPDH (1 : 1000; Cell Signaling Technology, #2118S) at 4°C overnight.

    Techniques: Expressing

    Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.

    Journal: Neuroreport

    Article Title: Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

    doi: 10.1097/WNR.0000000000002067

    Figure Lengend Snippet: Electroacupuncture alters the activation of TLR4 and NLRP3 in hippocampal microglia of PSCI rats. Quantification of (a) NLRP3 + /Iba-1 + and (b) TLR4 + /Iba-1 + colabeling cells in different groups. Representative immunofluorescence images showing colocalization of (c) NLRP3 (red) and Iba-1 (green); (d) TLR4 (red) and Iba-1 (green). Scale bar: 50 µm. Data are represented as mean ± SEM. ( n = 5). * P < 0.05; *** P < 0.001; # P < 0.01; ## P < 0.0001.

    Article Snippet: The first primary antibody TLR4 (1 : 500; Cell Signaling Technology, Massachusetts, USA, #13674S), NLRP3 (1 : 500; Abcam, London, UK, ab270449), was added and incubated in a wet box at 4°C overnight, then washed away with PBS 5 min ×3 times.

    Techniques: Activation Assay, Immunofluorescence

    Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.

    Journal: Neuroreport

    Article Title: Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

    doi: 10.1097/WNR.0000000000002067

    Figure Lengend Snippet: Electroacupuncture inhibits the expression levels of TLR4/NF-κB/NLRP3 signaling pathway in hippocampus of PSCI rats. Effects of the relative protein expression levels in hippocampus. (a) Electrophoretogram, (b) TLR4, (c) MyD88, (d) NF-κB, (e) NLRP3. Data are represented as mean ± SEM. All the experiments were repeated three times ( n = 5). * P < 0.05; *** P < 0.001.

    Article Snippet: The first primary antibody TLR4 (1 : 500; Cell Signaling Technology, Massachusetts, USA, #13674S), NLRP3 (1 : 500; Abcam, London, UK, ab270449), was added and incubated in a wet box at 4°C overnight, then washed away with PBS 5 min ×3 times.

    Techniques: Expressing

    Effect of TLR2 and TLR4 on CF-mediated activation of macrophages in RAW264.7 cells. RAW264.7 cells were pretreated with C29 (TLR2 inhibitor, 100 µM) or TAK (TLR4 inhibitor, 5 µM) for 2 h and then co-treated with CF (200 µg/ml) for 24 h. (A) The level of NO, (B) reverse transcription PCR analysis of iNOS, IL-1β and TNF-α, and (C) phagocytotic activity in RAW264.7 cells treated with CF for 24 h in the presence of C29 or TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. TLR, toll-like receptor; CF, Chrysosplenium flagelliferum ; TAK, TAK-242; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; CON, control; DM, DMSO group.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Anti‑obesity and immunostimulatory activity of Chrysosplenium flagelliferum in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells

    doi: 10.3892/etm.2024.12604

    Figure Lengend Snippet: Effect of TLR2 and TLR4 on CF-mediated activation of macrophages in RAW264.7 cells. RAW264.7 cells were pretreated with C29 (TLR2 inhibitor, 100 µM) or TAK (TLR4 inhibitor, 5 µM) for 2 h and then co-treated with CF (200 µg/ml) for 24 h. (A) The level of NO, (B) reverse transcription PCR analysis of iNOS, IL-1β and TNF-α, and (C) phagocytotic activity in RAW264.7 cells treated with CF for 24 h in the presence of C29 or TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. TLR, toll-like receptor; CF, Chrysosplenium flagelliferum ; TAK, TAK-242; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; CON, control; DM, DMSO group.

    Article Snippet: In addition, the cells were pretreated with C29 (100 µM, TLR2 inhibitor), TAK (5 µM, TLR4 inhibitor), PD (20 µM, ERK1/2 inhibior), SB (20 µM, p38 inhibitor), SP (20 µM, JNK inhibitor) or BAY (10 µM, NF-κB inhibitor) at 37˚C for 2 h and then co-treated with CF (200 µg/ml) at 37˚C for 24 h. To quantify NO levels, the culture medium was mixed with the Griess reagent (cat. no. G4410-10G; MilliporeSigma) and incubated for 15 min at ambient temperature.

    Techniques: Activation Assay, Reverse Transcription, Activity Assay, Control

    Effect of MAPK and NF-κB signaling pathways on CF-mediated activation of macrophages in RAW264.7 cells. (A) RAW264.7 cells were pretreated with PD (ERK1/2 inhibitor, 20 µM), SB (p38 inhibitor, 20 µM) or SP (JNK inhibitor, 20 µM) and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and (B) RT-PCR analysis of iNOS, IL-1β and TNF-α in RAW264.7 cells treated with CF in the presence of PD, SB or SP. (C) RAW264.7 cells were pretreated with BAY (NF-κB inhibitor, 10 µM), and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and RT-PCR analysis of iNOS, IL-1β, and TNF-α in RAW264.7 cells treated with CF for 24 h in the presence of BAY. (D) RAW264.7 cells were treated with CF (200 µM) for the indicated time-points. Western blot analysis of p-JNK, JNK, p-p65 and p65 in RAW264.7 cells treated with CF. (E) RAW264.7 cells were pretreated with TAK (TLR4 inhibitor, 5 µM) for 2 h and the co-treated with CF (200 µM) for 3 h. Western blot analysis of p-JNK, JNK, p-p65, and p65 in RAW264.7 cells treated with CF in the presence of TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. CF, Chrysosplenium flagelliferum ; PD, PD98059; SB, SB203580; SP, SP600125; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; BAY, BAY11-7082; p-, phosphorylated; JNK, c-Jun N-terminal kinase; TAK, TAK-242; CON, control; DM, DMSO group.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Anti‑obesity and immunostimulatory activity of Chrysosplenium flagelliferum in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells

    doi: 10.3892/etm.2024.12604

    Figure Lengend Snippet: Effect of MAPK and NF-κB signaling pathways on CF-mediated activation of macrophages in RAW264.7 cells. (A) RAW264.7 cells were pretreated with PD (ERK1/2 inhibitor, 20 µM), SB (p38 inhibitor, 20 µM) or SP (JNK inhibitor, 20 µM) and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and (B) RT-PCR analysis of iNOS, IL-1β and TNF-α in RAW264.7 cells treated with CF in the presence of PD, SB or SP. (C) RAW264.7 cells were pretreated with BAY (NF-κB inhibitor, 10 µM), and then co-treated with CF (200 µg/ml) for 24 h. The level of NO and RT-PCR analysis of iNOS, IL-1β, and TNF-α in RAW264.7 cells treated with CF for 24 h in the presence of BAY. (D) RAW264.7 cells were treated with CF (200 µM) for the indicated time-points. Western blot analysis of p-JNK, JNK, p-p65 and p65 in RAW264.7 cells treated with CF. (E) RAW264.7 cells were pretreated with TAK (TLR4 inhibitor, 5 µM) for 2 h and the co-treated with CF (200 µM) for 3 h. Western blot analysis of p-JNK, JNK, p-p65, and p65 in RAW264.7 cells treated with CF in the presence of TAK. * P#x003C;0.05 vs. CON. # P#x003C;0.05 vs. DM. CF, Chrysosplenium flagelliferum ; PD, PD98059; SB, SB203580; SP, SP600125; NO, nitric oxide; iNOS, inducible nitric oxide synthase; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; BAY, BAY11-7082; p-, phosphorylated; JNK, c-Jun N-terminal kinase; TAK, TAK-242; CON, control; DM, DMSO group.

    Article Snippet: In addition, the cells were pretreated with C29 (100 µM, TLR2 inhibitor), TAK (5 µM, TLR4 inhibitor), PD (20 µM, ERK1/2 inhibior), SB (20 µM, p38 inhibitor), SP (20 µM, JNK inhibitor) or BAY (10 µM, NF-κB inhibitor) at 37˚C for 2 h and then co-treated with CF (200 µg/ml) at 37˚C for 24 h. To quantify NO levels, the culture medium was mixed with the Griess reagent (cat. no. G4410-10G; MilliporeSigma) and incubated for 15 min at ambient temperature.

    Techniques: Activation Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

    a Histograms and bar graphs showing percentages of CD38+ and Egr2+ BMDM among all BMDM after M1- or M2-like activation, respectively (n = 3 biological replicates). Independent experiments in Supplementary Fig. . b Immunoblot analysis of WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . c Histograms and bar graph showing percentages of MyD88-expressing BMDM after PBS or LPS stimulation (n = 3 biological replicates). Results are pooled from 3 independent experiments with each 1 pair of mice; each point represents results from individual experiment. Independent experiment in Supplementary Fig. . d Confocal fluorescence microscopy images, showing MyD88 and TLR4 in WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . e Illustration of the experimental design in ( f – i ). Myris, myristoylation f Confocal fluorescence microcopy showing MyD88 and TLR4 in PBS-/LPS-treated WT or Lyz2 -cre BMDM transduced with MigR1-GFP or MigR1-GFP-MyrisMyD88. Cell sizes were visualized in bar graphs (WT MigR1-GFP: n = 4; Lyz2 -cre MigR1-GFP: n = 6; MigR1-GFP-MyrisMyD88: n = 3 biological replicates). Bar graph data are pooled from 3 individual experiments (more images in Supplementary Fig. ). g Representative flow cytometry dot plots, showing transduction efficiency in LPS-activated WT or Lyz2 -cre BMDM. More data in Supplementary Fig. . h Flow cytometry dot plots and bar graphs, showing percentages of FSC-A high , SSC-A high GFP + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . i Dot plots and bar graphs showing percentages of GFP + CD38 + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . Data are presented as mean ± SD ( a , c , f , h , i ). Statistical comparisons were performed with two-tailed unpaired Student’s t -tests ( a , f ; data points were normally distributed) and one-way ANOVA tests ( c , h , i ; for simultaneous comparisons of more than two groups). 9-week-old female and male ( a – d , f – i ) C57BL/6 mice were used. e Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Sphinganine recruits TLR4 adaptors in macrophages and promotes inflammation in murine models of sepsis and melanoma

    doi: 10.1038/s41467-024-50341-w

    Figure Lengend Snippet: a Histograms and bar graphs showing percentages of CD38+ and Egr2+ BMDM among all BMDM after M1- or M2-like activation, respectively (n = 3 biological replicates). Independent experiments in Supplementary Fig. . b Immunoblot analysis of WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . c Histograms and bar graph showing percentages of MyD88-expressing BMDM after PBS or LPS stimulation (n = 3 biological replicates). Results are pooled from 3 independent experiments with each 1 pair of mice; each point represents results from individual experiment. Independent experiment in Supplementary Fig. . d Confocal fluorescence microscopy images, showing MyD88 and TLR4 in WT or Lyz2 -cre BMDM after PBS or LPS stimulation. Independent experiments in Supplementary Fig. . e Illustration of the experimental design in ( f – i ). Myris, myristoylation f Confocal fluorescence microcopy showing MyD88 and TLR4 in PBS-/LPS-treated WT or Lyz2 -cre BMDM transduced with MigR1-GFP or MigR1-GFP-MyrisMyD88. Cell sizes were visualized in bar graphs (WT MigR1-GFP: n = 4; Lyz2 -cre MigR1-GFP: n = 6; MigR1-GFP-MyrisMyD88: n = 3 biological replicates). Bar graph data are pooled from 3 individual experiments (more images in Supplementary Fig. ). g Representative flow cytometry dot plots, showing transduction efficiency in LPS-activated WT or Lyz2 -cre BMDM. More data in Supplementary Fig. . h Flow cytometry dot plots and bar graphs, showing percentages of FSC-A high , SSC-A high GFP + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . i Dot plots and bar graphs showing percentages of GFP + CD38 + LPS-stimulated BMDM (n = 3 biological replicates). Independent experiment in Supplementary Fig. . Data are presented as mean ± SD ( a , c , f , h , i ). Statistical comparisons were performed with two-tailed unpaired Student’s t -tests ( a , f ; data points were normally distributed) and one-way ANOVA tests ( c , h , i ; for simultaneous comparisons of more than two groups). 9-week-old female and male ( a – d , f – i ) C57BL/6 mice were used. e Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Source data are provided as a file.

    Article Snippet: For surface antigen staining, TLR4-APC (Thermo Fisher Scientific, #MTS510) was added for 1–2 h on ice on a shaker, and cells were fixed with fixation buffer containing 4% paraformaldehyde and permeabilized with eBioscience permeabilization buffer.

    Techniques: Activation Assay, Western Blot, Expressing, Fluorescence, Microscopy, Transduction, Flow Cytometry, Two Tailed Test

    Illustration of the role of Sptlc2-derived sphinganine onto LPS-induced TLR4 signaling in macrophages. LPS induces Sptlc2-mediated sphinganine production and sphinganine interacts physically with TIRAP and MyD88 to recruit MyD88 to TLR4 in the macrophage membrane. This induces downstream signaling and results in M1-like activation-associated morphologic changes and cytokine release, mediating a strengthened acute inflammatory response. In absence of Sptlc2, MyD88 is not recruited to the membrane, preventing activation of downstream signaling. Figure 6 created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

    Journal: Nature Communications

    Article Title: Sphinganine recruits TLR4 adaptors in macrophages and promotes inflammation in murine models of sepsis and melanoma

    doi: 10.1038/s41467-024-50341-w

    Figure Lengend Snippet: Illustration of the role of Sptlc2-derived sphinganine onto LPS-induced TLR4 signaling in macrophages. LPS induces Sptlc2-mediated sphinganine production and sphinganine interacts physically with TIRAP and MyD88 to recruit MyD88 to TLR4 in the macrophage membrane. This induces downstream signaling and results in M1-like activation-associated morphologic changes and cytokine release, mediating a strengthened acute inflammatory response. In absence of Sptlc2, MyD88 is not recruited to the membrane, preventing activation of downstream signaling. Figure 6 created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

    Article Snippet: For surface antigen staining, TLR4-APC (Thermo Fisher Scientific, #MTS510) was added for 1–2 h on ice on a shaker, and cells were fixed with fixation buffer containing 4% paraformaldehyde and permeabilized with eBioscience permeabilization buffer.

    Techniques: Derivative Assay, Membrane, Activation Assay

    Genotype frequencies of toll-like receptor family gene and their association with Colon cancer

    Journal: BMC Cancer

    Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk

    doi: 10.1186/s12885-024-12604-z

    Figure Lengend Snippet: Genotype frequencies of toll-like receptor family gene and their association with Colon cancer

    Article Snippet: The PCR procedure involved an initial denaturation step at 94 °C for 5 m, followed by 30 cycles of denaturation at 94 °C for 20s, annealing at 59 °C for 30s, and extension at 72 °C for 35s, with a final extension at 72 °C for 5 m. The PCR products for amplifying TLR4 rs1927914 (524 bp) and rs7869402 (102 bp) were digested by NSi I and Alu I (NEB, Ipswich, USA), respectively, and the resulting fragments were analyzed by agarose-gel electrophoresis.

    Techniques:

    Stratified analysis between genotypes of  TLR4 rs1927914  and colon cancer risk

    Journal: BMC Cancer

    Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk

    doi: 10.1186/s12885-024-12604-z

    Figure Lengend Snippet: Stratified analysis between genotypes of TLR4 rs1927914 and colon cancer risk

    Article Snippet: The PCR procedure involved an initial denaturation step at 94 °C for 5 m, followed by 30 cycles of denaturation at 94 °C for 20s, annealing at 59 °C for 30s, and extension at 72 °C for 35s, with a final extension at 72 °C for 5 m. The PCR products for amplifying TLR4 rs1927914 (524 bp) and rs7869402 (102 bp) were digested by NSi I and Alu I (NEB, Ipswich, USA), respectively, and the resulting fragments were analyzed by agarose-gel electrophoresis.

    Techniques:

    The functional analysis of TLR4 rs1927914 polymorphism. ( A ) Luciferase expression of two constructers (pGL3-rs1927914G and pGL3-rs1927914A) in HCT116 and LOVO cells co-transfected with pRL-SV40 to standardize transfection efficiency. Fold increase was measured by setting the activity of the empty pGL3-Basic vector as 1. * P < 0.05 ** P < 0.01 compared with each of the construct counterparts. ( B ) Electrophoretic mobility shift assays with biotin-labeled oligonucleotide probes containing TLR4 rs1927914 A or G allele. Nuclear extracts were incubated with 5’-Biotin-TCTAGGACTTAGCAT A CAAATATTCCTGTT-3’ (A probe, lanes 1–3) or 5’-Biotin-TCTAGGACTTAGCAT G CAAATATTCCTGTT (G probe, lanes 4–6). Lanes 1 and 4 show the gel mobilities of the biotin-labeled probes without nuclear extracts; lanes 2 and 5 show the mobilities of the biotin-labeled probes with nuclear extracts in the absence of unlabeled probes. The binding specificity was confirmed by competing the biotin-labeled A or G probe with a 100-fold molar excess of unlabeled A (lane 3) or G probe (lane 6). ( C ) Transcription factor prediction using JASPAR and AliBaba showed that rs1927914 was consistent with the binding sequence of Oct-1

    Journal: BMC Cancer

    Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk

    doi: 10.1186/s12885-024-12604-z

    Figure Lengend Snippet: The functional analysis of TLR4 rs1927914 polymorphism. ( A ) Luciferase expression of two constructers (pGL3-rs1927914G and pGL3-rs1927914A) in HCT116 and LOVO cells co-transfected with pRL-SV40 to standardize transfection efficiency. Fold increase was measured by setting the activity of the empty pGL3-Basic vector as 1. * P < 0.05 ** P < 0.01 compared with each of the construct counterparts. ( B ) Electrophoretic mobility shift assays with biotin-labeled oligonucleotide probes containing TLR4 rs1927914 A or G allele. Nuclear extracts were incubated with 5’-Biotin-TCTAGGACTTAGCAT A CAAATATTCCTGTT-3’ (A probe, lanes 1–3) or 5’-Biotin-TCTAGGACTTAGCAT G CAAATATTCCTGTT (G probe, lanes 4–6). Lanes 1 and 4 show the gel mobilities of the biotin-labeled probes without nuclear extracts; lanes 2 and 5 show the mobilities of the biotin-labeled probes with nuclear extracts in the absence of unlabeled probes. The binding specificity was confirmed by competing the biotin-labeled A or G probe with a 100-fold molar excess of unlabeled A (lane 3) or G probe (lane 6). ( C ) Transcription factor prediction using JASPAR and AliBaba showed that rs1927914 was consistent with the binding sequence of Oct-1

    Article Snippet: The PCR procedure involved an initial denaturation step at 94 °C for 5 m, followed by 30 cycles of denaturation at 94 °C for 20s, annealing at 59 °C for 30s, and extension at 72 °C for 35s, with a final extension at 72 °C for 5 m. The PCR products for amplifying TLR4 rs1927914 (524 bp) and rs7869402 (102 bp) were digested by NSi I and Alu I (NEB, Ipswich, USA), respectively, and the resulting fragments were analyzed by agarose-gel electrophoresis.

    Techniques: Functional Assay, Luciferase, Expressing, Transfection, Activity Assay, Plasmid Preparation, Construct, Electrophoretic Mobility Shift Assay, Labeling, Incubation, Binding Assay, Sequencing