texas red  (Vector Laboratories)


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    Vector Laboratories texas red
    Texas Red, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    texas red  (Vector Laboratories)


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    Vector Laboratories texas red
    Texas Red, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    tomato lectin antibody  (Vector Laboratories)


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    Vector Laboratories tomato lectin antibody
    The effect of acetate supplementation on microglia reactivity in rats subjected to neuroborreliosis . Panel ( A ) shows a representative image of the western blot analysis measuring integrin alpha M chain (CD11b) and immunoreactivity, a surrogate marker of microglia activation, and panel ( B ) represents the composite measure of the western blot analysis. Panel ( C ) and ( D ) show representative images of <t>tomato</t> <t>lectin-positive</t> microglia (Arrows) in infected rats treated with either water or glyceryl triacetate (GTA), respectively. The values in panel B represent the means ± SD expressed in units of fold-increase over naïve rats where ‘a’ represents a significant increase compared to naïve rats and ‘b’ represents a significant difference comparing infected rats treated with GTA to infected rats treated with water (n = 6, P ≤0.05). The reticule in panels C and D represent a length of 10 μm.
    Tomato Lectin Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tomato lectin antibody/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    1) Product Images from "Acetate supplementation reduces microglia activation and brain interleukin-1β levels in a rat model of Lyme neuroborreliosis"

    Article Title: Acetate supplementation reduces microglia activation and brain interleukin-1β levels in a rat model of Lyme neuroborreliosis

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-9-249

    The effect of acetate supplementation on microglia reactivity in rats subjected to neuroborreliosis . Panel ( A ) shows a representative image of the western blot analysis measuring integrin alpha M chain (CD11b) and immunoreactivity, a surrogate marker of microglia activation, and panel ( B ) represents the composite measure of the western blot analysis. Panel ( C ) and ( D ) show representative images of tomato lectin-positive microglia (Arrows) in infected rats treated with either water or glyceryl triacetate (GTA), respectively. The values in panel B represent the means ± SD expressed in units of fold-increase over naïve rats where ‘a’ represents a significant increase compared to naïve rats and ‘b’ represents a significant difference comparing infected rats treated with GTA to infected rats treated with water (n = 6, P ≤0.05). The reticule in panels C and D represent a length of 10 μm.
    Figure Legend Snippet: The effect of acetate supplementation on microglia reactivity in rats subjected to neuroborreliosis . Panel ( A ) shows a representative image of the western blot analysis measuring integrin alpha M chain (CD11b) and immunoreactivity, a surrogate marker of microglia activation, and panel ( B ) represents the composite measure of the western blot analysis. Panel ( C ) and ( D ) show representative images of tomato lectin-positive microglia (Arrows) in infected rats treated with either water or glyceryl triacetate (GTA), respectively. The values in panel B represent the means ± SD expressed in units of fold-increase over naïve rats where ‘a’ represents a significant increase compared to naïve rats and ‘b’ represents a significant difference comparing infected rats treated with GTA to infected rats treated with water (n = 6, P ≤0.05). The reticule in panels C and D represent a length of 10 μm.

    Techniques Used: Western Blot, Marker, Activation Assay, Infection

    vectashield  (Vector Laboratories)


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    Vector Laboratories vectashield
    Vectashield, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    fluorescent marker tomato lectin  (Vector Laboratories)


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    Vector Laboratories fluorescent marker tomato lectin
    Neuroprotective effect of LA on a hemi-Parkinsonian animal model. Animals were injected unilaterally with 6-OHDA in the SNpc in combination or not with LA. The epifluorescence microscope images are ipsilateral coronal sections containing SNpc samples from the different experimental groups. ( A ) Representative images of the SNpc showing the presence of dopaminergic neurons labeled with tyrosine hydroxylase (TH, blue); astroglial cells stained with glial fibrillary acidic protein (GFAP, green) and <t>Tomato</t> <t>lectin</t> (red) as a marker for activated microglial cells. Scale bar, 200 μm. The right columns show greater magnification of the area delimited in the images in the left column (100 μm). ( B ) Quantification of the number of dopaminergic, astrocyte, and microglial cells shown in (A). ( C ) Analysis of the presence of degenerating neurons (green) in the SNpc measured using Fluoro-Jade B staining (DAPI was used as a nuclear stain). Scale bar, 100 μm. ( D ) Quantification of the number of Fluoro-Jade B (FJ-B) cells shown in ( C ). ( E ) Apoptotic neurons in the SNpc measured by the TUNEL assay. Scale bar, 100 μm. ( F ) Quantification of the number of TUNEL-positive cells in ( E ). The values in ( B , D , F ) represent the mean ± SD, expressed as the percentage of positive cells in the SNpc, given a particular marker, from three different experiments. There were four animals/experiment/experimental group and five independent sections per animal. *** p < 0.001.
    Fluorescent Marker Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent marker tomato lectin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    fluorescent marker tomato lectin - by Bioz Stars, 2024-05
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    Images

    1) Product Images from "Neuroprotective and Anti-Inflammatory Effects of Linoleic Acid in Models of Parkinson’s Disease: The Implication of Lipid Droplets and Lipophagy"

    Article Title: Neuroprotective and Anti-Inflammatory Effects of Linoleic Acid in Models of Parkinson’s Disease: The Implication of Lipid Droplets and Lipophagy

    Journal: Cells

    doi: 10.3390/cells11152297

    Neuroprotective effect of LA on a hemi-Parkinsonian animal model. Animals were injected unilaterally with 6-OHDA in the SNpc in combination or not with LA. The epifluorescence microscope images are ipsilateral coronal sections containing SNpc samples from the different experimental groups. ( A ) Representative images of the SNpc showing the presence of dopaminergic neurons labeled with tyrosine hydroxylase (TH, blue); astroglial cells stained with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker for activated microglial cells. Scale bar, 200 μm. The right columns show greater magnification of the area delimited in the images in the left column (100 μm). ( B ) Quantification of the number of dopaminergic, astrocyte, and microglial cells shown in (A). ( C ) Analysis of the presence of degenerating neurons (green) in the SNpc measured using Fluoro-Jade B staining (DAPI was used as a nuclear stain). Scale bar, 100 μm. ( D ) Quantification of the number of Fluoro-Jade B (FJ-B) cells shown in ( C ). ( E ) Apoptotic neurons in the SNpc measured by the TUNEL assay. Scale bar, 100 μm. ( F ) Quantification of the number of TUNEL-positive cells in ( E ). The values in ( B , D , F ) represent the mean ± SD, expressed as the percentage of positive cells in the SNpc, given a particular marker, from three different experiments. There were four animals/experiment/experimental group and five independent sections per animal. *** p < 0.001.
    Figure Legend Snippet: Neuroprotective effect of LA on a hemi-Parkinsonian animal model. Animals were injected unilaterally with 6-OHDA in the SNpc in combination or not with LA. The epifluorescence microscope images are ipsilateral coronal sections containing SNpc samples from the different experimental groups. ( A ) Representative images of the SNpc showing the presence of dopaminergic neurons labeled with tyrosine hydroxylase (TH, blue); astroglial cells stained with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker for activated microglial cells. Scale bar, 200 μm. The right columns show greater magnification of the area delimited in the images in the left column (100 μm). ( B ) Quantification of the number of dopaminergic, astrocyte, and microglial cells shown in (A). ( C ) Analysis of the presence of degenerating neurons (green) in the SNpc measured using Fluoro-Jade B staining (DAPI was used as a nuclear stain). Scale bar, 100 μm. ( D ) Quantification of the number of Fluoro-Jade B (FJ-B) cells shown in ( C ). ( E ) Apoptotic neurons in the SNpc measured by the TUNEL assay. Scale bar, 100 μm. ( F ) Quantification of the number of TUNEL-positive cells in ( E ). The values in ( B , D , F ) represent the mean ± SD, expressed as the percentage of positive cells in the SNpc, given a particular marker, from three different experiments. There were four animals/experiment/experimental group and five independent sections per animal. *** p < 0.001.

    Techniques Used: Animal Model, Injection, Microscopy, Labeling, Staining, Marker, TUNEL Assay

    Neuroprotective and anti-inflammatory effect of LA on a hemiparkinsonian animal model. Representative images of coronal sections of the mouse SNpc showing the presence of dopaminergic neurons (TH, blue); astroglial cells labeled with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker of activated microglial cells, both in the contralateral (not injected) and ipsilateral (injected) hemisphere. Scale bar, 200 μm.
    Figure Legend Snippet: Neuroprotective and anti-inflammatory effect of LA on a hemiparkinsonian animal model. Representative images of coronal sections of the mouse SNpc showing the presence of dopaminergic neurons (TH, blue); astroglial cells labeled with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker of activated microglial cells, both in the contralateral (not injected) and ipsilateral (injected) hemisphere. Scale bar, 200 μm.

    Techniques Used: Animal Model, Labeling, Marker, Injection

    tomato lectin  (Vector Laboratories)


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    Vector Laboratories tomato lectin
    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of <t>lectin</t> <t>fluorescence</t> coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.
    Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Compromised Cortical-Hippocampal Network Function From Transient Hypertension: Linking Mid-Life Hypertension to Late Life Dementia Risk"

    Article Title: Compromised Cortical-Hippocampal Network Function From Transient Hypertension: Linking Mid-Life Hypertension to Late Life Dementia Risk

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2022.897206

    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of lectin fluorescence coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.
    Figure Legend Snippet: Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of lectin fluorescence coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.

    Techniques Used: Fluorescence, Western Blot, Expressing

    intravital dye labeling  (Vector Laboratories)


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    Vector Laboratories intravital dye labeling
    Intravital Dye Labeling, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    texas red labeled lycopersicon esculentum tomato lectin  (Vector Laboratories)


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    Vector Laboratories texas red labeled lycopersicon esculentum tomato lectin
    Texas Red Labeled Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lycopersicon esculentum lectin  (Vector Laboratories)


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    Vector Laboratories lycopersicon esculentum lectin
    Lycopersicon Esculentum Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    texas red  (Vector Laboratories)


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    Vector Laboratories texas red
    Texas Red, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lycopersicon esculentum tomato  (Vector Laboratories)


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    Vector Laboratories lycopersicon esculentum tomato
    Lycopersicon Esculentum Tomato, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories texas red
    Texas Red, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories tomato lectin antibody
    The effect of acetate supplementation on microglia reactivity in rats subjected to neuroborreliosis . Panel ( A ) shows a representative image of the western blot analysis measuring integrin alpha M chain (CD11b) and immunoreactivity, a surrogate marker of microglia activation, and panel ( B ) represents the composite measure of the western blot analysis. Panel ( C ) and ( D ) show representative images of <t>tomato</t> <t>lectin-positive</t> microglia (Arrows) in infected rats treated with either water or glyceryl triacetate (GTA), respectively. The values in panel B represent the means ± SD expressed in units of fold-increase over naïve rats where ‘a’ represents a significant increase compared to naïve rats and ‘b’ represents a significant difference comparing infected rats treated with GTA to infected rats treated with water (n = 6, P ≤0.05). The reticule in panels C and D represent a length of 10 μm.
    Tomato Lectin Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories vectashield
    The effect of acetate supplementation on microglia reactivity in rats subjected to neuroborreliosis . Panel ( A ) shows a representative image of the western blot analysis measuring integrin alpha M chain (CD11b) and immunoreactivity, a surrogate marker of microglia activation, and panel ( B ) represents the composite measure of the western blot analysis. Panel ( C ) and ( D ) show representative images of <t>tomato</t> <t>lectin-positive</t> microglia (Arrows) in infected rats treated with either water or glyceryl triacetate (GTA), respectively. The values in panel B represent the means ± SD expressed in units of fold-increase over naïve rats where ‘a’ represents a significant increase compared to naïve rats and ‘b’ represents a significant difference comparing infected rats treated with GTA to infected rats treated with water (n = 6, P ≤0.05). The reticule in panels C and D represent a length of 10 μm.
    Vectashield, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories fluorescent marker tomato lectin
    Neuroprotective effect of LA on a hemi-Parkinsonian animal model. Animals were injected unilaterally with 6-OHDA in the SNpc in combination or not with LA. The epifluorescence microscope images are ipsilateral coronal sections containing SNpc samples from the different experimental groups. ( A ) Representative images of the SNpc showing the presence of dopaminergic neurons labeled with tyrosine hydroxylase (TH, blue); astroglial cells stained with glial fibrillary acidic protein (GFAP, green) and <t>Tomato</t> <t>lectin</t> (red) as a marker for activated microglial cells. Scale bar, 200 μm. The right columns show greater magnification of the area delimited in the images in the left column (100 μm). ( B ) Quantification of the number of dopaminergic, astrocyte, and microglial cells shown in (A). ( C ) Analysis of the presence of degenerating neurons (green) in the SNpc measured using Fluoro-Jade B staining (DAPI was used as a nuclear stain). Scale bar, 100 μm. ( D ) Quantification of the number of Fluoro-Jade B (FJ-B) cells shown in ( C ). ( E ) Apoptotic neurons in the SNpc measured by the TUNEL assay. Scale bar, 100 μm. ( F ) Quantification of the number of TUNEL-positive cells in ( E ). The values in ( B , D , F ) represent the mean ± SD, expressed as the percentage of positive cells in the SNpc, given a particular marker, from three different experiments. There were four animals/experiment/experimental group and five independent sections per animal. *** p < 0.001.
    Fluorescent Marker Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories tomato lectin
    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of <t>lectin</t> <t>fluorescence</t> coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.
    Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories intravital dye labeling
    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of <t>lectin</t> <t>fluorescence</t> coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.
    Intravital Dye Labeling, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories texas red labeled lycopersicon esculentum tomato lectin
    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of <t>lectin</t> <t>fluorescence</t> coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.
    Texas Red Labeled Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of <t>lectin</t> <t>fluorescence</t> coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.
    Lycopersicon Esculentum Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of <t>lectin</t> <t>fluorescence</t> coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.
    Lycopersicon Esculentum Tomato, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The effect of acetate supplementation on microglia reactivity in rats subjected to neuroborreliosis . Panel ( A ) shows a representative image of the western blot analysis measuring integrin alpha M chain (CD11b) and immunoreactivity, a surrogate marker of microglia activation, and panel ( B ) represents the composite measure of the western blot analysis. Panel ( C ) and ( D ) show representative images of tomato lectin-positive microglia (Arrows) in infected rats treated with either water or glyceryl triacetate (GTA), respectively. The values in panel B represent the means ± SD expressed in units of fold-increase over naïve rats where ‘a’ represents a significant increase compared to naïve rats and ‘b’ represents a significant difference comparing infected rats treated with GTA to infected rats treated with water (n = 6, P ≤0.05). The reticule in panels C and D represent a length of 10 μm.

    Journal: Journal of Neuroinflammation

    Article Title: Acetate supplementation reduces microglia activation and brain interleukin-1β levels in a rat model of Lyme neuroborreliosis

    doi: 10.1186/1742-2094-9-249

    Figure Lengend Snippet: The effect of acetate supplementation on microglia reactivity in rats subjected to neuroborreliosis . Panel ( A ) shows a representative image of the western blot analysis measuring integrin alpha M chain (CD11b) and immunoreactivity, a surrogate marker of microglia activation, and panel ( B ) represents the composite measure of the western blot analysis. Panel ( C ) and ( D ) show representative images of tomato lectin-positive microglia (Arrows) in infected rats treated with either water or glyceryl triacetate (GTA), respectively. The values in panel B represent the means ± SD expressed in units of fold-increase over naïve rats where ‘a’ represents a significant increase compared to naïve rats and ‘b’ represents a significant difference comparing infected rats treated with GTA to infected rats treated with water (n = 6, P ≤0.05). The reticule in panels C and D represent a length of 10 μm.

    Article Snippet: Glyceryl triacetate, 2-mercaptoethanol, buffers, fixative solutions, a Cy3-labeled monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (C9205) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and Texas Red™-labeled tomato lectin antibody (TL-1176), normal horse serum, normal goat serum, and Vectashield™ were obtained from Vector Laboratories (Burlingame, CA, USA).

    Techniques: Western Blot, Marker, Activation Assay, Infection

    Neuroprotective effect of LA on a hemi-Parkinsonian animal model. Animals were injected unilaterally with 6-OHDA in the SNpc in combination or not with LA. The epifluorescence microscope images are ipsilateral coronal sections containing SNpc samples from the different experimental groups. ( A ) Representative images of the SNpc showing the presence of dopaminergic neurons labeled with tyrosine hydroxylase (TH, blue); astroglial cells stained with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker for activated microglial cells. Scale bar, 200 μm. The right columns show greater magnification of the area delimited in the images in the left column (100 μm). ( B ) Quantification of the number of dopaminergic, astrocyte, and microglial cells shown in (A). ( C ) Analysis of the presence of degenerating neurons (green) in the SNpc measured using Fluoro-Jade B staining (DAPI was used as a nuclear stain). Scale bar, 100 μm. ( D ) Quantification of the number of Fluoro-Jade B (FJ-B) cells shown in ( C ). ( E ) Apoptotic neurons in the SNpc measured by the TUNEL assay. Scale bar, 100 μm. ( F ) Quantification of the number of TUNEL-positive cells in ( E ). The values in ( B , D , F ) represent the mean ± SD, expressed as the percentage of positive cells in the SNpc, given a particular marker, from three different experiments. There were four animals/experiment/experimental group and five independent sections per animal. *** p < 0.001.

    Journal: Cells

    Article Title: Neuroprotective and Anti-Inflammatory Effects of Linoleic Acid in Models of Parkinson’s Disease: The Implication of Lipid Droplets and Lipophagy

    doi: 10.3390/cells11152297

    Figure Lengend Snippet: Neuroprotective effect of LA on a hemi-Parkinsonian animal model. Animals were injected unilaterally with 6-OHDA in the SNpc in combination or not with LA. The epifluorescence microscope images are ipsilateral coronal sections containing SNpc samples from the different experimental groups. ( A ) Representative images of the SNpc showing the presence of dopaminergic neurons labeled with tyrosine hydroxylase (TH, blue); astroglial cells stained with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker for activated microglial cells. Scale bar, 200 μm. The right columns show greater magnification of the area delimited in the images in the left column (100 μm). ( B ) Quantification of the number of dopaminergic, astrocyte, and microglial cells shown in (A). ( C ) Analysis of the presence of degenerating neurons (green) in the SNpc measured using Fluoro-Jade B staining (DAPI was used as a nuclear stain). Scale bar, 100 μm. ( D ) Quantification of the number of Fluoro-Jade B (FJ-B) cells shown in ( C ). ( E ) Apoptotic neurons in the SNpc measured by the TUNEL assay. Scale bar, 100 μm. ( F ) Quantification of the number of TUNEL-positive cells in ( E ). The values in ( B , D , F ) represent the mean ± SD, expressed as the percentage of positive cells in the SNpc, given a particular marker, from three different experiments. There were four animals/experiment/experimental group and five independent sections per animal. *** p < 0.001.

    Article Snippet: Some sections were stained with the fluorescent marker Tomato Lectin (1:150, Vector Labs, Newark, CA, USA, TL1176).

    Techniques: Animal Model, Injection, Microscopy, Labeling, Staining, Marker, TUNEL Assay

    Neuroprotective and anti-inflammatory effect of LA on a hemiparkinsonian animal model. Representative images of coronal sections of the mouse SNpc showing the presence of dopaminergic neurons (TH, blue); astroglial cells labeled with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker of activated microglial cells, both in the contralateral (not injected) and ipsilateral (injected) hemisphere. Scale bar, 200 μm.

    Journal: Cells

    Article Title: Neuroprotective and Anti-Inflammatory Effects of Linoleic Acid in Models of Parkinson’s Disease: The Implication of Lipid Droplets and Lipophagy

    doi: 10.3390/cells11152297

    Figure Lengend Snippet: Neuroprotective and anti-inflammatory effect of LA on a hemiparkinsonian animal model. Representative images of coronal sections of the mouse SNpc showing the presence of dopaminergic neurons (TH, blue); astroglial cells labeled with glial fibrillary acidic protein (GFAP, green) and Tomato lectin (red) as a marker of activated microglial cells, both in the contralateral (not injected) and ipsilateral (injected) hemisphere. Scale bar, 200 μm.

    Article Snippet: Some sections were stained with the fluorescent marker Tomato Lectin (1:150, Vector Labs, Newark, CA, USA, TL1176).

    Techniques: Animal Model, Labeling, Marker, Injection

    Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of lectin fluorescence coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.

    Journal: Frontiers in Neuroscience

    Article Title: Compromised Cortical-Hippocampal Network Function From Transient Hypertension: Linking Mid-Life Hypertension to Late Life Dementia Risk

    doi: 10.3389/fnins.2022.897206

    Figure Lengend Snippet: Transient hypertension results in irreversible compromise to the cerebrovasculature despite extended recovery. (A) Representative images of lectin fluorescence coverage in PFC; scale bar = 500 μm. (B) Quantification of lectin area coverage (left) and number of vessels (right) in PFC: Both lectin coverage ( p = 0.036) and number of lectin-positive vessels ( p = 0.032) are significantly lower in L-NAME group. (C) Representative images of lectin fluorescence coverage in HIPP; scale bar = 750 μm. (D) Quantification of lectin coverage (left) and number of vessels (right) in HIPP: Both lectin coverage ( p = 0.593) and number of vessels (0.700) are not significantly different between groups ( p = 0.593). (E) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in PFC: AQP4 immunoreactivity coverage is higher in L-NAME group ( p = 0.036); scale bar = 500 μm. (F) Representative images (left and middle) and quantification (right) of AQP4 fluorescence coverage in HIPP: AQP4 immunoreactivity coverage is unchanged ( p = 0.999). (G) Representative immunoblots (top) and immunoblot quantification of AQP4 protein expression normalized to GAPDH in PFC (middle) and HIPP (bottom); L-NAME increased AQP4 protein expression in PFC ( p = 0.002) but not in HIPP ( p = 0.879). Scale bar = 500 μm. Significance was calculated by t -test. * and ** denote p < 0.05 and p < 0.01 respectively; n = 8 rats per group.

    Article Snippet: For fluorescent labeling, floating sections were blocked for 1 h, probed with primary antibodies overnight at 4°C, then incubated with both fluorescence-tagged secondary antibodies and fluorescence-tagged tomato lectin (Vector #TL-1176-1, 1:250) for 2 h. Both the block and antibody incubations were diluted in PBS-0.5% Triton-0.5% bovine serum albumin.

    Techniques: Fluorescence, Western Blot, Expressing