titanium taq dna pol  (TaKaRa)

 
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    Name:
    Titanium Taq DNA Polymerase
    Description:
    Titanium Taq DNA Polymerase is a blend of a specially engineered Taq and an antibody for integrated hot start PCR which prevents non specific amplification and primer dimer formation Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples including bacterial and plasmid DNA cDNA and complex genomic DNA It gives higher PCR product yield than other polymerases and is active over a wide range of Mg2 concentrations Titanium Taq is available in several formats
    Catalog Number:
    639242
    Price:
    None
    Size:
    1 000 Rxns
    Category:
    Titanium Taq DNA Polymerase Titanium Taq products High yield PCR PCR
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    Structured Review

    TaKaRa titanium taq dna pol
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Titanium Taq DNA Polymerase is a blend of a specially engineered Taq and an antibody for integrated hot start PCR which prevents non specific amplification and primer dimer formation Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples including bacterial and plasmid DNA cDNA and complex genomic DNA It gives higher PCR product yield than other polymerases and is active over a wide range of Mg2 concentrations Titanium Taq is available in several formats
    https://www.bioz.com/result/titanium taq dna pol/product/TaKaRa
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    titanium taq dna pol - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Highly specific unnatural base pair systems as a third base pair for PCR amplification"

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkr1068

    Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Figure Legend Snippet: Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .

    Techniques Used: Sequencing, Polymerase Chain Reaction, Amplification

    2) Product Images from "Highly specific unnatural base pair systems as a third base pair for PCR amplification"

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkr1068

    Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Figure Legend Snippet: Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .

    Techniques Used: Sequencing, Polymerase Chain Reaction, Amplification

    Related Articles

    Clone Assay:

    Article Title: Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone
    Article Snippet: Bisulphite sequencing of genomic DNA from iPSC clones was used to assess methylation status of OCT4 and NANOG promoter. .. PCR with primers OCT4-mF3/R3 and NANOG-mF3/R3, which were used by other investigators, was carried out using Titanium Taq polymerase (Clontech Laboratories, Mountain View, CA): The cycling conditions were 95°C 10 minutes, followed by 40 cycles of 95 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, and finally 72 °C for 7 minutes.

    Amplification:

    Article Title: Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer
    Article Snippet: .. A total of 250 ng of genomic DNA was digested with Nsp I (250 K) or both Nsp I and Sty I in independent parallel reactions (SNP6.0), subjected to restriction enzymes, ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech, Palo Alto, CA). .. The PCR products were then quantified, fragmented, end-labeled, and hybridized onto a GeneChip Human Mapping 250 K Nsp Array or a Genome-wide Human SNP6.0 Array.

    Article Title: Characterisation and Validation of Insertions and Deletions in 173 Patient Exomes
    Article Snippet: .. Validation of the INDELs 5 ng of each DNA sample were amplified in a 20 µl reaction mixture containing 0.2 mM of each dNTP (Takara), 1× Titanium Taq PCR Buffer (Clontech), 0.25× Titanium Taq DNA Polymerase (Clontech) and 0.2 µM of forward and reverse primers designed to specifically amplify each DNA fragment containing a putative indel. .. The PCR program included an initial step of denaturation at 95°C for 1 minute followed by 35 cycles of amplification characterized by the following profile: 95°C for 30″, 60°C for 30″, 68°C for 30″ and a final extension step at 68°C for 3 minutes.

    Article Title: Vascular Smooth Muscle Modulates Endothelial Control of Vasoreactivity via Reactive Oxygen Species Production through Myoendothelial Communications
    Article Snippet: Triplicate PCR reactions were assembled in 0.1-ml strip tubes containing cDNA from 10 ng of total RNA, 0.2 µl of 50× Titanium Taq DNA Polymerase combined to its buffer (Clontech Laboratories), 1 mM dNTP, each of the appropriate primer (Sigma Genosys; see for concentrations and sequences), and 0.5× SYBR Green (Molecular Probes). .. Data collection was performed after each extension step, at a temperature of at least 3.5°C lower than the melting temperature of the amplicon (generally between 80–85°C) to eliminate non-specific fluorescence signal.

    Article Title: A Dominant Allele of Arabidopsis Pectin-Binding Wall-Associated Kinase Induces a Stress Response Suppressed by MPK6 but Not MPK3 Mutations
    Article Snippet: PCR analysis of alleles was carried out using the following primers and Titanium™ Taq (Clontech 639208) using the manufacturers’ buffers and conditions, and genomic DNA extracted as described ( ). .. Amplified DNA using primers listed below was analyzed by agarose gel electrophoresis and staining with ethidium bromide: Actin: Actin-F 5'-AAAGGATGCTTATGTTGGCG-3', Actin-Rev 5'-GAAAGAGTAACCACGCTCGG-3'; MPK3 wild-type: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', MPK3R 5'-TGCTGCACTTCTAACCGTATGTTGGATTG-3'; MPK3null: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', P745 5'-AACGTCCGCAATGTGTTATTAAGTTGTC-3'; MPK6 wild-type: MPK6-F2 5'-GCCTCAGATGCCTGGGATTGAGAATATTC-3', MPK6-RT-R2 5'-AGAGTGGCTTACGGTCCATTAACTCCAATG-3'; MPK6null: MPK6-F2 and p745; WAK2cTAP: WAK2-2405F 5'-GAGCGATGTTTATAGTTTTGGGGTCGTCC-3', CTAP303R 5'-GGTTTGCGGCGCTCACTG-3'.

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: A multiplex PCR (PCR1) was first carried out for the simultaneous amplification of S. littoralis bestrophins and two control genes (RpL8 and SlitOrco). .. Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ).

    Article Title: Combined megaplex TCR isolation and SMART-based real-time quantitation methods for quantitating antigen-specific T cell clones in mycobacterial infection
    Article Snippet: .. Primary PCR amplification was performed in a single tube with 50 μl volume containing mixtures of ext.Vβ family-specific primers (24 families, 0.25 μM each), mixtures of ext.Jβ-specific primers (13 segments, 0.25 μM each), 1 × Titanium DNA Polymerase (Clontech) and 12–20 μl of DNA template sample. ..

    Synthesized:

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: DNA fragments comprising only the natural bases were synthesized or purchased from Invitrogen. .. The following thermostable DNA polymerases were purchased: Deep Vent (exo+ ), Deep Vent (exo− ), Vent (exo+ ), N9°, Phusion HF and Taq DNA pols from New England Biolabs; AccuPrime Pfx ™ and Pfx50 DNA pols from Invitrogen, Pfu DNA pol from Promega; Pwo SY DNA pol from Roche; TITANIUM Taq DNA pol from Clontech.

    Real-time Polymerase Chain Reaction:

    Article Title: Vascular Smooth Muscle Modulates Endothelial Control of Vasoreactivity via Reactive Oxygen Species Production through Myoendothelial Communications
    Article Snippet: Paragraph title: Real-time Quantitative Polymerase Chain Reaction (PCR) ... Triplicate PCR reactions were assembled in 0.1-ml strip tubes containing cDNA from 10 ng of total RNA, 0.2 µl of 50× Titanium Taq DNA Polymerase combined to its buffer (Clontech Laboratories), 1 mM dNTP, each of the appropriate primer (Sigma Genosys; see for concentrations and sequences), and 0.5× SYBR Green (Molecular Probes).

    Incubation:

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: Then 20 µM Random primers, Oligo dTs (1 µl each, Clontech), 10 mM dNTPs Mix (1 µl, Clontech) and MMLV Reverse Transcriptase (200 U, 1 µl, Clontech) were added and the final 20 µl-solution was successively incubated 10 min at 25°C, 50 min at 42°C and 15 min at 70°C. .. Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ).

    Stripping Membranes:

    Article Title: Vascular Smooth Muscle Modulates Endothelial Control of Vasoreactivity via Reactive Oxygen Species Production through Myoendothelial Communications
    Article Snippet: .. Triplicate PCR reactions were assembled in 0.1-ml strip tubes containing cDNA from 10 ng of total RNA, 0.2 µl of 50× Titanium Taq DNA Polymerase combined to its buffer (Clontech Laboratories), 1 mM dNTP, each of the appropriate primer (Sigma Genosys; see for concentrations and sequences), and 0.5× SYBR Green (Molecular Probes). ..

    Genome Wide:

    Article Title: Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer
    Article Snippet: Array-based comparative genomic hybridization The Genome-wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA) was used to perform array-CGH on genomic DNA from each of the PC cell lines as described previously [ ]. .. A total of 250 ng of genomic DNA was digested with Nsp I (250 K) or both Nsp I and Sty I in independent parallel reactions (SNP6.0), subjected to restriction enzymes, ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech, Palo Alto, CA).

    Modification:

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: The syntheses of d Ds TP, NH2 -hx-d Px TP and Cy5-hx-d Px TP was described previously ( , , ), and the syntheses of the other modified-d Px TPs are described in the Supplementary Data section . .. The following thermostable DNA polymerases were purchased: Deep Vent (exo+ ), Deep Vent (exo− ), Vent (exo+ ), N9°, Phusion HF and Taq DNA pols from New England Biolabs; AccuPrime Pfx ™ and Pfx50 DNA pols from Invitrogen, Pfu DNA pol from Promega; Pwo SY DNA pol from Roche; TITANIUM Taq DNA pol from Clontech.

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: .. Indeed, our recent study showed that TITANIUM Taq DNA pol exhibited high selectivity and efficiency in a one-way incorporation of modified d Px TP opposite Ds in templates, and is useful for the unnatural base incorporation in a primer region in PCR ( ). .. These results suggest that the TITANIUM Taq and Taq DNA pols efficiently incorporate d Px TP into DNA opposite Ds , but are not useful to incorporate d Ds TP into DNA opposite Px .

    Bisulfite Sequencing:

    Article Title: Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone
    Article Snippet: Bisulphite sequencing of genomic DNA from iPSC clones was used to assess methylation status of OCT4 and NANOG promoter. .. PCR with primers OCT4-mF3/R3 and NANOG-mF3/R3, which were used by other investigators, was carried out using Titanium Taq polymerase (Clontech Laboratories, Mountain View, CA): The cycling conditions were 95°C 10 minutes, followed by 40 cycles of 95 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, and finally 72 °C for 7 minutes.

    Methylation:

    Article Title: Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone
    Article Snippet: The conversion of unmethylated cytosines to uracil was carried out using EZ DNA Methylation-Gold Kit (ZYMO Research, Irvine, CA). .. PCR with primers OCT4-mF3/R3 and NANOG-mF3/R3, which were used by other investigators, was carried out using Titanium Taq polymerase (Clontech Laboratories, Mountain View, CA): The cycling conditions were 95°C 10 minutes, followed by 40 cycles of 95 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, and finally 72 °C for 7 minutes.

    Polymerase Chain Reaction:

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: .. Indeed, our recent study showed that TITANIUM Taq DNA pol exhibited high selectivity and efficiency in a one-way incorporation of modified d Px TP opposite Ds in templates, and is useful for the unnatural base incorporation in a primer region in PCR ( ). .. These results suggest that the TITANIUM Taq and Taq DNA pols efficiently incorporate d Px TP into DNA opposite Ds , but are not useful to incorporate d Ds TP into DNA opposite Px .

    Article Title: Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone
    Article Snippet: .. PCR with primers OCT4-mF3/R3 and NANOG-mF3/R3, which were used by other investigators, was carried out using Titanium Taq polymerase (Clontech Laboratories, Mountain View, CA): The cycling conditions were 95°C 10 minutes, followed by 40 cycles of 95 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, and finally 72 °C for 7 minutes. .. The PCR products were cloned into a pJET1.2 vector (Fermentas, Glen Burnie, MD) and sequenced by MCLAB (San Francisco, CA).

    Article Title: Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer
    Article Snippet: .. A total of 250 ng of genomic DNA was digested with Nsp I (250 K) or both Nsp I and Sty I in independent parallel reactions (SNP6.0), subjected to restriction enzymes, ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech, Palo Alto, CA). .. The PCR products were then quantified, fragmented, end-labeled, and hybridized onto a GeneChip Human Mapping 250 K Nsp Array or a Genome-wide Human SNP6.0 Array.

    Article Title: Characterisation and Validation of Insertions and Deletions in 173 Patient Exomes
    Article Snippet: .. Validation of the INDELs 5 ng of each DNA sample were amplified in a 20 µl reaction mixture containing 0.2 mM of each dNTP (Takara), 1× Titanium Taq PCR Buffer (Clontech), 0.25× Titanium Taq DNA Polymerase (Clontech) and 0.2 µM of forward and reverse primers designed to specifically amplify each DNA fragment containing a putative indel. .. The PCR program included an initial step of denaturation at 95°C for 1 minute followed by 35 cycles of amplification characterized by the following profile: 95°C for 30″, 60°C for 30″, 68°C for 30″ and a final extension step at 68°C for 3 minutes.

    Article Title: Demonstration of Array-Based Analysis for Highly Multiplexed PCR Assays
    Article Snippet: .. Approximately 100 to 200 ng of genomic DNA was used for each single round PCR, using the MPAD primer set with Titanium Taq polymerase and buffers (Clonetech) or BIOMED-2 primer sets with AmpliTaq Gold polymerase and buffers (Applied Biosystems). ..

    Article Title: Vascular Smooth Muscle Modulates Endothelial Control of Vasoreactivity via Reactive Oxygen Species Production through Myoendothelial Communications
    Article Snippet: .. Triplicate PCR reactions were assembled in 0.1-ml strip tubes containing cDNA from 10 ng of total RNA, 0.2 µl of 50× Titanium Taq DNA Polymerase combined to its buffer (Clontech Laboratories), 1 mM dNTP, each of the appropriate primer (Sigma Genosys; see for concentrations and sequences), and 0.5× SYBR Green (Molecular Probes). ..

    Article Title: The pht4;1-3 mutant line contains a loss of function allele in the Fatty Acid Desaturase 7 gene caused by a remnant inactivated selection marker—a cautionary tale
    Article Snippet: Samples were dried in room temperature and the pellet containing DNA was reconstituted in 150 µL H2 O. PCRs was performed using a BioRad S1000 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with Titanium Taq polymerase (Clontech Laboratories, Inc., Mountain View, CA, USA) or AccuPrime Pfx DNA Polymerase (Invitrogen, Life Technologies, Carlsbad, CA, USA) according to manufacturers’ instructions with primers listed in . .. PCR condition were as follows: 2 min initial denaturation at 95 °C, 30 s denaturation at 95 °C, 30 s annealing at 58 °C, 0.5–3 min extension at 68 °C (35 cycles), and final extension for 3–5 min. PCR products were analyzed by agarose gel (1–2% agarose; Seakem LE, Lonza, Switzerland) electrophoresis after staining with GelStar™ (Lonza, Basel, Switzerland).

    Article Title: A Dominant Allele of Arabidopsis Pectin-Binding Wall-Associated Kinase Induces a Stress Response Suppressed by MPK6 but Not MPK3 Mutations
    Article Snippet: .. PCR analysis of alleles was carried out using the following primers and Titanium™ Taq (Clontech 639208) using the manufacturers’ buffers and conditions, and genomic DNA extracted as described ( ). .. Amplified DNA using primers listed below was analyzed by agarose gel electrophoresis and staining with ethidium bromide: Actin: Actin-F 5'-AAAGGATGCTTATGTTGGCG-3', Actin-Rev 5'-GAAAGAGTAACCACGCTCGG-3'; MPK3 wild-type: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', MPK3R 5'-TGCTGCACTTCTAACCGTATGTTGGATTG-3'; MPK3null: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', P745 5'-AACGTCCGCAATGTGTTATTAAGTTGTC-3'; MPK6 wild-type: MPK6-F2 5'-GCCTCAGATGCCTGGGATTGAGAATATTC-3', MPK6-RT-R2 5'-AGAGTGGCTTACGGTCCATTAACTCCAATG-3'; MPK6null: MPK6-F2 and p745; WAK2cTAP: WAK2-2405F 5'-GAGCGATGTTTATAGTTTTGGGGTCGTCC-3', CTAP303R 5'-GGTTTGCGGCGCTCACTG-3'.

    Article Title: Thyroid-stimulating hormone induces a Wnt-dependent, feed-forward loop for osteoblastogenesis in embryonic stem cell cultures
    Article Snippet: Paragraph title: Semiquantitative PCR. ... RT-PCRs for stem cell and osteoblast markers was performed using Titanium Taq polymerase (Clontech Laboratories).

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: .. Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ). .. After 1 min at 94°C, samples were processed for 35 cycles of 95°C for 30 s, 60°C for 30 s, and 68°C for 1 min. Then, a nested PCR was performed on 2 µl of PCR1 products with 46 µl of a reaction mix containing 5 µl 10 X Titanium Taq buffer, 1 µl 50 X Titanium Taq DNA polymerase, and 1 µl of dNTPs (10 mM) and an antisense primer specific to each gene (SlitBest1-SC.R2, SlitBest2-SC.R2, SlitOrco-SC.R2 and RpL8-SC.R2).

    Article Title: Combined megaplex TCR isolation and SMART-based real-time quantitation methods for quantitating antigen-specific T cell clones in mycobacterial infection
    Article Snippet: .. Primary PCR amplification was performed in a single tube with 50 μl volume containing mixtures of ext.Vβ family-specific primers (24 families, 0.25 μM each), mixtures of ext.Jβ-specific primers (13 segments, 0.25 μM each), 1 × Titanium DNA Polymerase (Clontech) and 12–20 μl of DNA template sample. ..

    Sequencing:

    Article Title: Characterisation and Validation of Insertions and Deletions in 173 Patient Exomes
    Article Snippet: Validation of the INDELs 5 ng of each DNA sample were amplified in a 20 µl reaction mixture containing 0.2 mM of each dNTP (Takara), 1× Titanium Taq PCR Buffer (Clontech), 0.25× Titanium Taq DNA Polymerase (Clontech) and 0.2 µM of forward and reverse primers designed to specifically amplify each DNA fragment containing a putative indel. .. Sequence data obtained from each PCR fragment were aligned to the corresponding reference sequences with the software Sequencher 4.9 (Gene Codes).

    Article Title: Demonstration of Array-Based Analysis for Highly Multiplexed PCR Assays
    Article Snippet: Paragraph title: Sequence Analysis ... Approximately 100 to 200 ng of genomic DNA was used for each single round PCR, using the MPAD primer set with Titanium Taq polymerase and buffers (Clonetech) or BIOMED-2 primer sets with AmpliTaq Gold polymerase and buffers (Applied Biosystems).

    Hybridization:

    Article Title: Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer
    Article Snippet: Paragraph title: Array-based comparative genomic hybridization ... A total of 250 ng of genomic DNA was digested with Nsp I (250 K) or both Nsp I and Sty I in independent parallel reactions (SNP6.0), subjected to restriction enzymes, ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech, Palo Alto, CA).

    Nucleic Acid Electrophoresis:

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: DNA fragments were purified by gel electrophoresis. .. The following thermostable DNA polymerases were purchased: Deep Vent (exo+ ), Deep Vent (exo− ), Vent (exo+ ), N9°, Phusion HF and Taq DNA pols from New England Biolabs; AccuPrime Pfx ™ and Pfx50 DNA pols from Invitrogen, Pfu DNA pol from Promega; Pwo SY DNA pol from Roche; TITANIUM Taq DNA pol from Clontech.

    Fluorescence:

    Article Title: Vascular Smooth Muscle Modulates Endothelial Control of Vasoreactivity via Reactive Oxygen Species Production through Myoendothelial Communications
    Article Snippet: Triplicate PCR reactions were assembled in 0.1-ml strip tubes containing cDNA from 10 ng of total RNA, 0.2 µl of 50× Titanium Taq DNA Polymerase combined to its buffer (Clontech Laboratories), 1 mM dNTP, each of the appropriate primer (Sigma Genosys; see for concentrations and sequences), and 0.5× SYBR Green (Molecular Probes). .. Data collection was performed after each extension step, at a temperature of at least 3.5°C lower than the melting temperature of the amplicon (generally between 80–85°C) to eliminate non-specific fluorescence signal.

    Magnetic Beads:

    Article Title: Characterisation and Validation of Insertions and Deletions in 173 Patient Exomes
    Article Snippet: Validation of the INDELs 5 ng of each DNA sample were amplified in a 20 µl reaction mixture containing 0.2 mM of each dNTP (Takara), 1× Titanium Taq PCR Buffer (Clontech), 0.25× Titanium Taq DNA Polymerase (Clontech) and 0.2 µM of forward and reverse primers designed to specifically amplify each DNA fragment containing a putative indel. .. Each PCR product was purified with Agencourt® AMPure® XP (Beckman Coulter) magnetic beads and subsequently sequenced by standard dideoxy-sequencing on Applied Biosystem 3730 with both forward and reverse primers.

    Transferring:

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: In the whole-cell configuration, the cytosol of ORNs exhibiting a CaC current was harvested within the pipette filled of RNase-free solution containing (in mM) 140 CsCl, 1 CaCl2 , 2 MgCl2 , and 10 HEPES (pH 7.4, 325 mosmol/L). .. Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ).

    Purification:

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: DNA fragments were purified by gel electrophoresis. .. The following thermostable DNA polymerases were purchased: Deep Vent (exo+ ), Deep Vent (exo− ), Vent (exo+ ), N9°, Phusion HF and Taq DNA pols from New England Biolabs; AccuPrime Pfx ™ and Pfx50 DNA pols from Invitrogen, Pfu DNA pol from Promega; Pwo SY DNA pol from Roche; TITANIUM Taq DNA pol from Clontech.

    Article Title: Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone
    Article Snippet: Genomic DNA was purified from human iPSCs by DNeasy Kit (Qiagen, Valencia, CA). .. PCR with primers OCT4-mF3/R3 and NANOG-mF3/R3, which were used by other investigators, was carried out using Titanium Taq polymerase (Clontech Laboratories, Mountain View, CA): The cycling conditions were 95°C 10 minutes, followed by 40 cycles of 95 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, and finally 72 °C for 7 minutes.

    Article Title: Characterisation and Validation of Insertions and Deletions in 173 Patient Exomes
    Article Snippet: Validation of the INDELs 5 ng of each DNA sample were amplified in a 20 µl reaction mixture containing 0.2 mM of each dNTP (Takara), 1× Titanium Taq PCR Buffer (Clontech), 0.25× Titanium Taq DNA Polymerase (Clontech) and 0.2 µM of forward and reverse primers designed to specifically amplify each DNA fragment containing a putative indel. .. Each PCR product was purified with Agencourt® AMPure® XP (Beckman Coulter) magnetic beads and subsequently sequenced by standard dideoxy-sequencing on Applied Biosystem 3730 with both forward and reverse primers.

    Article Title: Demonstration of Array-Based Analysis for Highly Multiplexed PCR Assays
    Article Snippet: Approximately 100 to 200 ng of genomic DNA was used for each single round PCR, using the MPAD primer set with Titanium Taq polymerase and buffers (Clonetech) or BIOMED-2 primer sets with AmpliTaq Gold polymerase and buffers (Applied Biosystems). .. Approximately 100 to 200 ng of genomic DNA was used for each single round PCR, using the MPAD primer set with Titanium Taq polymerase and buffers (Clonetech) or BIOMED-2 primer sets with AmpliTaq Gold polymerase and buffers (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: Paragraph title: Single-Cell RT-PCR ... Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ).

    Nested PCR:

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ). .. After 1 min at 94°C, samples were processed for 35 cycles of 95°C for 30 s, 60°C for 30 s, and 68°C for 1 min. Then, a nested PCR was performed on 2 µl of PCR1 products with 46 µl of a reaction mix containing 5 µl 10 X Titanium Taq buffer, 1 µl 50 X Titanium Taq DNA polymerase, and 1 µl of dNTPs (10 mM) and an antisense primer specific to each gene (SlitBest1-SC.R2, SlitBest2-SC.R2, SlitOrco-SC.R2 and RpL8-SC.R2).

    Plasmid Preparation:

    Article Title: Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone
    Article Snippet: PCR with primers OCT4-mF3/R3 and NANOG-mF3/R3, which were used by other investigators, was carried out using Titanium Taq polymerase (Clontech Laboratories, Mountain View, CA): The cycling conditions were 95°C 10 minutes, followed by 40 cycles of 95 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, and finally 72 °C for 7 minutes. .. The PCR products were cloned into a pJET1.2 vector (Fermentas, Glen Burnie, MD) and sequenced by MCLAB (San Francisco, CA).

    Software:

    Article Title: Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer
    Article Snippet: A total of 250 ng of genomic DNA was digested with Nsp I (250 K) or both Nsp I and Sty I in independent parallel reactions (SNP6.0), subjected to restriction enzymes, ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech, Palo Alto, CA). .. After washing and staining in Fluidics Station 450 (Affymetrix), the arrays were scanned to generate CEL files using the GeneChip Scanner 3000 and GeneChip Operating Software, ver.1.4.

    Article Title: Characterisation and Validation of Insertions and Deletions in 173 Patient Exomes
    Article Snippet: Validation of the INDELs 5 ng of each DNA sample were amplified in a 20 µl reaction mixture containing 0.2 mM of each dNTP (Takara), 1× Titanium Taq PCR Buffer (Clontech), 0.25× Titanium Taq DNA Polymerase (Clontech) and 0.2 µM of forward and reverse primers designed to specifically amplify each DNA fragment containing a putative indel. .. Sequence data obtained from each PCR fragment were aligned to the corresponding reference sequences with the software Sequencher 4.9 (Gene Codes).

    SYBR Green Assay:

    Article Title: Vascular Smooth Muscle Modulates Endothelial Control of Vasoreactivity via Reactive Oxygen Species Production through Myoendothelial Communications
    Article Snippet: .. Triplicate PCR reactions were assembled in 0.1-ml strip tubes containing cDNA from 10 ng of total RNA, 0.2 µl of 50× Titanium Taq DNA Polymerase combined to its buffer (Clontech Laboratories), 1 mM dNTP, each of the appropriate primer (Sigma Genosys; see for concentrations and sequences), and 0.5× SYBR Green (Molecular Probes). ..

    Multiplex Assay:

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: A multiplex PCR (PCR1) was first carried out for the simultaneous amplification of S. littoralis bestrophins and two control genes (RpL8 and SlitOrco). .. Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ).

    Agarose Gel Electrophoresis:

    Article Title: The pht4;1-3 mutant line contains a loss of function allele in the Fatty Acid Desaturase 7 gene caused by a remnant inactivated selection marker—a cautionary tale
    Article Snippet: Samples were dried in room temperature and the pellet containing DNA was reconstituted in 150 µL H2 O. PCRs was performed using a BioRad S1000 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with Titanium Taq polymerase (Clontech Laboratories, Inc., Mountain View, CA, USA) or AccuPrime Pfx DNA Polymerase (Invitrogen, Life Technologies, Carlsbad, CA, USA) according to manufacturers’ instructions with primers listed in . .. PCR condition were as follows: 2 min initial denaturation at 95 °C, 30 s denaturation at 95 °C, 30 s annealing at 58 °C, 0.5–3 min extension at 68 °C (35 cycles), and final extension for 3–5 min. PCR products were analyzed by agarose gel (1–2% agarose; Seakem LE, Lonza, Switzerland) electrophoresis after staining with GelStar™ (Lonza, Basel, Switzerland).

    Article Title: A Dominant Allele of Arabidopsis Pectin-Binding Wall-Associated Kinase Induces a Stress Response Suppressed by MPK6 but Not MPK3 Mutations
    Article Snippet: PCR analysis of alleles was carried out using the following primers and Titanium™ Taq (Clontech 639208) using the manufacturers’ buffers and conditions, and genomic DNA extracted as described ( ). .. Amplified DNA using primers listed below was analyzed by agarose gel electrophoresis and staining with ethidium bromide: Actin: Actin-F 5'-AAAGGATGCTTATGTTGGCG-3', Actin-Rev 5'-GAAAGAGTAACCACGCTCGG-3'; MPK3 wild-type: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', MPK3R 5'-TGCTGCACTTCTAACCGTATGTTGGATTG-3'; MPK3null: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', P745 5'-AACGTCCGCAATGTGTTATTAAGTTGTC-3'; MPK6 wild-type: MPK6-F2 5'-GCCTCAGATGCCTGGGATTGAGAATATTC-3', MPK6-RT-R2 5'-AGAGTGGCTTACGGTCCATTAACTCCAATG-3'; MPK6null: MPK6-F2 and p745; WAK2cTAP: WAK2-2405F 5'-GAGCGATGTTTATAGTTTTGGGGTCGTCC-3', CTAP303R 5'-GGTTTGCGGCGCTCACTG-3'.

    Article Title: Combined megaplex TCR isolation and SMART-based real-time quantitation methods for quantitating antigen-specific T cell clones in mycobacterial infection
    Article Snippet: Primary PCR amplification was performed in a single tube with 50 μl volume containing mixtures of ext.Vβ family-specific primers (24 families, 0.25 μM each), mixtures of ext.Jβ-specific primers (13 segments, 0.25 μM each), 1 × Titanium DNA Polymerase (Clontech) and 12–20 μl of DNA template sample. .. The PCR products were separated and visualized with ethidium bromide on a 2% agarose gel.

    Electrophoresis:

    Article Title: The pht4;1-3 mutant line contains a loss of function allele in the Fatty Acid Desaturase 7 gene caused by a remnant inactivated selection marker—a cautionary tale
    Article Snippet: Samples were dried in room temperature and the pellet containing DNA was reconstituted in 150 µL H2 O. PCRs was performed using a BioRad S1000 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with Titanium Taq polymerase (Clontech Laboratories, Inc., Mountain View, CA, USA) or AccuPrime Pfx DNA Polymerase (Invitrogen, Life Technologies, Carlsbad, CA, USA) according to manufacturers’ instructions with primers listed in . .. PCR condition were as follows: 2 min initial denaturation at 95 °C, 30 s denaturation at 95 °C, 30 s annealing at 58 °C, 0.5–3 min extension at 68 °C (35 cycles), and final extension for 3–5 min. PCR products were analyzed by agarose gel (1–2% agarose; Seakem LE, Lonza, Switzerland) electrophoresis after staining with GelStar™ (Lonza, Basel, Switzerland).

    Patch Clamp:

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: Patch-clamp recordings were performed from 10-to-15-day-old cultures as previously described . .. Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ).

    Knock-Out:

    Article Title: The pht4;1-3 mutant line contains a loss of function allele in the Fatty Acid Desaturase 7 gene caused by a remnant inactivated selection marker—a cautionary tale
    Article Snippet: The fad7-1 knock-out line (NASC ID N8042) was acquired from the Nottingham Arabidopsis Stock Center ( ; ). .. Samples were dried in room temperature and the pellet containing DNA was reconstituted in 150 µL H2 O. PCRs was performed using a BioRad S1000 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with Titanium Taq polymerase (Clontech Laboratories, Inc., Mountain View, CA, USA) or AccuPrime Pfx DNA Polymerase (Invitrogen, Life Technologies, Carlsbad, CA, USA) according to manufacturers’ instructions with primers listed in .

    Staining:

    Article Title: Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone
    Article Snippet: After sectioning, samples were embedded in paraffin and stained with hematoxylin and eosin and analyzed by a board certified pathologist. .. PCR with primers OCT4-mF3/R3 and NANOG-mF3/R3, which were used by other investigators, was carried out using Titanium Taq polymerase (Clontech Laboratories, Mountain View, CA): The cycling conditions were 95°C 10 minutes, followed by 40 cycles of 95 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, and finally 72 °C for 7 minutes.

    Article Title: Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer
    Article Snippet: A total of 250 ng of genomic DNA was digested with Nsp I (250 K) or both Nsp I and Sty I in independent parallel reactions (SNP6.0), subjected to restriction enzymes, ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech, Palo Alto, CA). .. After washing and staining in Fluidics Station 450 (Affymetrix), the arrays were scanned to generate CEL files using the GeneChip Scanner 3000 and GeneChip Operating Software, ver.1.4.

    Article Title: The pht4;1-3 mutant line contains a loss of function allele in the Fatty Acid Desaturase 7 gene caused by a remnant inactivated selection marker—a cautionary tale
    Article Snippet: Samples were dried in room temperature and the pellet containing DNA was reconstituted in 150 µL H2 O. PCRs was performed using a BioRad S1000 Thermal Cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with Titanium Taq polymerase (Clontech Laboratories, Inc., Mountain View, CA, USA) or AccuPrime Pfx DNA Polymerase (Invitrogen, Life Technologies, Carlsbad, CA, USA) according to manufacturers’ instructions with primers listed in . .. PCR condition were as follows: 2 min initial denaturation at 95 °C, 30 s denaturation at 95 °C, 30 s annealing at 58 °C, 0.5–3 min extension at 68 °C (35 cycles), and final extension for 3–5 min. PCR products were analyzed by agarose gel (1–2% agarose; Seakem LE, Lonza, Switzerland) electrophoresis after staining with GelStar™ (Lonza, Basel, Switzerland).

    Article Title: A Dominant Allele of Arabidopsis Pectin-Binding Wall-Associated Kinase Induces a Stress Response Suppressed by MPK6 but Not MPK3 Mutations
    Article Snippet: PCR analysis of alleles was carried out using the following primers and Titanium™ Taq (Clontech 639208) using the manufacturers’ buffers and conditions, and genomic DNA extracted as described ( ). .. Amplified DNA using primers listed below was analyzed by agarose gel electrophoresis and staining with ethidium bromide: Actin: Actin-F 5'-AAAGGATGCTTATGTTGGCG-3', Actin-Rev 5'-GAAAGAGTAACCACGCTCGG-3'; MPK3 wild-type: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', MPK3R 5'-TGCTGCACTTCTAACCGTATGTTGGATTG-3'; MPK3null: MPK3F 5'-CCGAGCAATCTTCTGTTGAACGCGAATTG-3', P745 5'-AACGTCCGCAATGTGTTATTAAGTTGTC-3'; MPK6 wild-type: MPK6-F2 5'-GCCTCAGATGCCTGGGATTGAGAATATTC-3', MPK6-RT-R2 5'-AGAGTGGCTTACGGTCCATTAACTCCAATG-3'; MPK6null: MPK6-F2 and p745; WAK2cTAP: WAK2-2405F 5'-GAGCGATGTTTATAGTTTTGGGGTCGTCC-3', CTAP303R 5'-GGTTTGCGGCGCTCACTG-3'.

    Article Title: Thyroid-stimulating hormone induces a Wnt-dependent, feed-forward loop for osteoblastogenesis in embryonic stem cell cultures
    Article Snippet: RT-PCRs for stem cell and osteoblast markers was performed using Titanium Taq polymerase (Clontech Laboratories). .. The amplified PCR products were separated on a 2% agarose gels and visualized by ethidium bromide staining.

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    TaKaRa titanium taq dna pol
    Sequencing analysis of the PCR products after 15 cycles of PCR by each <t>DNA</t> polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) <t>TITANIUM</t> <t>Taq</t> DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .
    Titanium Taq Dna Pol, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .

    Journal: Nucleic Acids Research

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification

    doi: 10.1093/nar/gkr1068

    Figure Lengend Snippet: Sequencing analysis of the PCR products after 15 cycles of PCR by each DNA polymerase: ( A ) Deep Vent DNA pol (exo + ); ( B ) AccuPrime Pfx DNA pol; ( C ) Pfx50 DNA pol; ( D ) Pfu DNA pol; ( E ) Deep Vent DNA pol (exo − ); ( F ) TITANIUM Taq DNA pol. Sequencing reactions were performed in the presence of d Pa′ TP (left panels) or dd Pa′ TP (right panels) in each figure. The blue arrow indicates the original unnatural base position. The percentages indicated in the right panels for Ds -temp 1 and Ds -temp 4 are the retention rates of the Ds–Px pair in the amplified products after 15 cycles of PCR. The details of the calculation method for the retention rates are provided in the Supplementary Data section .

    Article Snippet: Indeed, our recent study showed that TITANIUM Taq DNA pol exhibited high selectivity and efficiency in a one-way incorporation of modified d Px TP opposite Ds in templates, and is useful for the unnatural base incorporation in a primer region in PCR ( ).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification