tissue deparaffinization (Ventana Medical)

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Ventana Medical tissue deparaffinization
Tissue Deparaffinization, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tissue deparaffinization/product/Ventana Medical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

tissue deparaffinization - by Bioz Stars, 2021-03

86/100 stars

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Immunohistochemistry:

Article Title: COL11A1 confers chemoresistance on ovarian cancer cells through the activation of Akt/c/EBPβ pathway and PDK1 stabilization
Article Snippet: .. Briefly, IHC staining of COL11A1 protein was performed on deparaffinized tissue sections of formalin-fixed material after microwave-enhanced epitope retrieval based on the standard automated procedure (Ventana XT autostainer; Ventana Medical Systems, Tucson, AZ). .. The primary rabbit polyclonal anti-COL11A1 antibody (SC-68853, Santa Cruz Biotechnology) was used at a 1:20 dilution.

Article Title: Investigating the association between radiological images and the pathology of rectal cancer treated with neoadjuvant chemotherapy
Article Snippet: .. Immunohistochemistry Immunohistochemistry was performed on deparaffinized tissue sections using the standard avidin-biotin-peroxidase complex method with an automated immunostainer (Benchmark XT; Ventana Medical Systems, Inc.). .. The formalin fixation conditions were the same as those used for H & E staining.

Staining:

Article Title: Activation of the AXL Kinase Causes Resistance to EGFR-Targeted Therapy in Lung Cancer
Article Snippet: Expression was examined by standard immunohistochemistry using 4um thick sections of matched, paired specimens. .. Deparaffinized tissue sections were stained following the manufacturer’s protocol at a dilution of 1:500 on a Ventana Dixcovery XT automated stainer. .. EGFR T790M mutation and MET am plification were analyzed using previously described sequencing and FISH protocols, respectively, and vimentin expression by IHC using established MET hods and clinical scoring criteria.

Avidin-Biotin Assay:

In Situ Hybridization:

Article Title: Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 gene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)
Article Snippet: Paraffin tissue sections on glass slides were baked at 65°C for 20 minutes prior to the deparaffinization step with EZ Prep™ (Ventana) at 75°C for 16 minutes. .. Deparaffinized tissue sections were pretreated with a combination of heat treatment with Reaction Buffer (Tris-based pH 7.6 solution, Ventana) and ISH Protease 2 or ISH Protease 3 (Ventana) to unmask DNA targets. .. Pretreatment conditions were chosen for optimal signal to noise ratio and tissue morphology preservation for the xenograft control slides as well as clinical case tissue slides.

Blocking Assay:

Article Title: Pharmacological inhibition of KIT activates MET signaling in gastrointestinal stromal tumors
Article Snippet: Intratumoral hypoxia was detected by i.p. injection of pimonidazole (60 mg/kg, Hypoxyprobe-1) 1h prior to sacrifice of KitV558del/+ mice. .. Pimonidazole was detected with a Discovery XT processor (Ventana Medical Systems) using deparaffinized tissue sections after antigen retrieval with CC1 buffer (Ventana) and blocking for 30 minutes with Background Buster solution (Innovex). .. Anti-pimonidazole mouse monoclonal antibody (Hypoxyprobe Inc.) was applied at 0.12 µg/ml and sections were incubated for 5h, followed by 1h incubation with biotinylated horse anti-mouse IgG (Vector Labs, cat# MKB-22258) at 1:200 dilution, then Streptavidin-HRP D (part of DABMap kit, Ventana), and followed by incubation with Tyramide Alexa Fluor 488 (Invitrogen, cat# ).