human cell line time  (ATCC)


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    ATCC human cell line time
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Human Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p"

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    Journal: Cells

    doi: 10.3390/cells10112843

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Figure Legend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).

    Techniques Used: Next-Generation Sequencing

    The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.
    Figure Legend Snippet: The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.

    Techniques Used: Cell Culture, Standard Deviation

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.
    Figure Legend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.

    Techniques Used: Isolation, Digital PCR, Expressing, Software

    human cell line time  (ATCC)


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    ATCC human cell line time
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Human Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cell line time/product/ATCC
    Average 94 stars, based on 1 article reviews
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    human cell line time - by Bioz Stars, 2024-02
    94/100 stars

    Images

    1) Product Images from "Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p"

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    Journal: Cells

    doi: 10.3390/cells10112843

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Figure Legend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).

    Techniques Used: Next-Generation Sequencing

    The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.
    Figure Legend Snippet: The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.

    Techniques Used: Cell Culture, Standard Deviation

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.
    Figure Legend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.

    Techniques Used: Isolation, Digital PCR, Expressing, Software

    time crl 4025 cell lines  (ATCC)


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    ATCC time crl 4025 cell lines
    Time Crl 4025 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    time cell line atcc crl 4025 authentication  (ATCC)


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    ATCC time cell line atcc crl 4025 authentication
    Time Cell Line Atcc Crl 4025 Authentication, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human cell line time  (ATCC)


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    ATCC human cell line time
    Effect direction indicated by qPCR and ddPCR. Human endothelial cells of the cell line <t>TIME</t> (ATCC <t>number:</t> <t>CRL-4025)</t> were stimulated for 24 h with TNF-α, IL-1β, and INF-γ in concentrations of 100 ng/ml each. The measured values are presented as mean values ± standard deviation. qPCR was performed in 5 independent experiments using 9 technical replicates each and ddPCR analysis was performed in 5 independent experiments using 3 technical replicates each. * symbolizes significant differences. ACE1 = angiotensin converting enzyme 1, ADRA1B = alpha-adrenergic receptor type 1B, ADRA1D = alpha-adrenergic receptor type 1D, ADRB2 = beta-adrenergic receptor type 2, ATIP1 = angiotensin II receptor interacting protein 1, ATRAP = angiotensin II type 1 receptor associated protein, EDNRA = endothelin receptor type A, EDNRB = endothelin receptor type B
    Human Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors"

    Article Title: Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors

    Journal: Biological Procedures Online

    doi: 10.1186/s12575-021-00144-w

    Effect direction indicated by qPCR and ddPCR. Human endothelial cells of the cell line TIME (ATCC number: CRL-4025) were stimulated for 24 h with TNF-α, IL-1β, and INF-γ in concentrations of 100 ng/ml each. The measured values are presented as mean values ± standard deviation. qPCR was performed in 5 independent experiments using 9 technical replicates each and ddPCR analysis was performed in 5 independent experiments using 3 technical replicates each. * symbolizes significant differences. ACE1 = angiotensin converting enzyme 1, ADRA1B = alpha-adrenergic receptor type 1B, ADRA1D = alpha-adrenergic receptor type 1D, ADRB2 = beta-adrenergic receptor type 2, ATIP1 = angiotensin II receptor interacting protein 1, ATRAP = angiotensin II type 1 receptor associated protein, EDNRA = endothelin receptor type A, EDNRB = endothelin receptor type B
    Figure Legend Snippet: Effect direction indicated by qPCR and ddPCR. Human endothelial cells of the cell line TIME (ATCC number: CRL-4025) were stimulated for 24 h with TNF-α, IL-1β, and INF-γ in concentrations of 100 ng/ml each. The measured values are presented as mean values ± standard deviation. qPCR was performed in 5 independent experiments using 9 technical replicates each and ddPCR analysis was performed in 5 independent experiments using 3 technical replicates each. * symbolizes significant differences. ACE1 = angiotensin converting enzyme 1, ADRA1B = alpha-adrenergic receptor type 1B, ADRA1D = alpha-adrenergic receptor type 1D, ADRB2 = beta-adrenergic receptor type 2, ATIP1 = angiotensin II receptor interacting protein 1, ATRAP = angiotensin II type 1 receptor associated protein, EDNRA = endothelin receptor type A, EDNRB = endothelin receptor type B

    Techniques Used: Standard Deviation

    human cell line time  (ATCC)


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    ATCC human cell line time
    Human Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    microvascular endothelial cell line time  (ATCC)


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    ATCC microvascular endothelial cell line time
    Microvascular Endothelial Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    shakt1 stable cell lines telomerase  (ATCC)


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    ATCC shakt1 stable cell lines telomerase
    (A) Representative confocal images showing immunofluorescence staining of VE-cadherin on HMEC monolayers transfected with either scrambled ShRNA or ShRNA targeting Akt1. (B) Representative Western blot images and band densitometry quantification of stable ShControl and <t>ShAkt1</t> HMEC lysates showing reduced Akt1 expression in ShAkt1 HMEC compared to ShControl HMEC (n=3). (C) Quantification of the gap area in control and Akt1 knockdown HMEC monolayers normalized to the total area (n=4). (D) Quantification of the number of gaps in control and Akt1 knockdown HMEC monolayers normalized to the number of nuclei per field (n=4). (E) Representative confocal images showing VE-cadherin staining on the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment. (F) Quantification of the number of gaps in the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment (n=4). Data are represented as mean ± SD. *P<0.01, #P<0.05, scale bar 20 μm.
    Shakt1 Stable Cell Lines Telomerase, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Modulation of long-term endothelial-barrier integrity is conditional to the cross-talk between Akt and Src signaling"

    Article Title: Modulation of long-term endothelial-barrier integrity is conditional to the cross-talk between Akt and Src signaling

    Journal: Journal of cellular physiology

    doi: 10.1002/jcp.25791

    (A) Representative confocal images showing immunofluorescence staining of VE-cadherin on HMEC monolayers transfected with either scrambled ShRNA or ShRNA targeting Akt1. (B) Representative Western blot images and band densitometry quantification of stable ShControl and ShAkt1 HMEC lysates showing reduced Akt1 expression in ShAkt1 HMEC compared to ShControl HMEC (n=3). (C) Quantification of the gap area in control and Akt1 knockdown HMEC monolayers normalized to the total area (n=4). (D) Quantification of the number of gaps in control and Akt1 knockdown HMEC monolayers normalized to the number of nuclei per field (n=4). (E) Representative confocal images showing VE-cadherin staining on the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment. (F) Quantification of the number of gaps in the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment (n=4). Data are represented as mean ± SD. *P<0.01, #P<0.05, scale bar 20 μm.
    Figure Legend Snippet: (A) Representative confocal images showing immunofluorescence staining of VE-cadherin on HMEC monolayers transfected with either scrambled ShRNA or ShRNA targeting Akt1. (B) Representative Western blot images and band densitometry quantification of stable ShControl and ShAkt1 HMEC lysates showing reduced Akt1 expression in ShAkt1 HMEC compared to ShControl HMEC (n=3). (C) Quantification of the gap area in control and Akt1 knockdown HMEC monolayers normalized to the total area (n=4). (D) Quantification of the number of gaps in control and Akt1 knockdown HMEC monolayers normalized to the number of nuclei per field (n=4). (E) Representative confocal images showing VE-cadherin staining on the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment. (F) Quantification of the number of gaps in the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment (n=4). Data are represented as mean ± SD. *P<0.01, #P<0.05, scale bar 20 μm.

    Techniques Used: Immunofluorescence, Staining, Transfection, shRNA, Western Blot, Expressing

    (A–B) Representative Western blot images and corresponding bar graph of Akt Ser473 phosphorylation and total-Akt in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (C–D) Representative Western blot images and corresponding bar graph of Src Tyr416 phosphorylation and total-Src in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (E–F) Real-time changes and a bar graph showing the quantification of changes in the ShControl and ShAkt1 HMEC-barrier resistance upon treatment with either vehicle (PBS) or 5 ng/ml TGFβ1 as measured using ECIS equipment. Data are represented as mean ± SD. (n=3), *p<0.01.
    Figure Legend Snippet: (A–B) Representative Western blot images and corresponding bar graph of Akt Ser473 phosphorylation and total-Akt in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (C–D) Representative Western blot images and corresponding bar graph of Src Tyr416 phosphorylation and total-Src in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (E–F) Real-time changes and a bar graph showing the quantification of changes in the ShControl and ShAkt1 HMEC-barrier resistance upon treatment with either vehicle (PBS) or 5 ng/ml TGFβ1 as measured using ECIS equipment. Data are represented as mean ± SD. (n=3), *p<0.01.

    Techniques Used: Western Blot

    microvascular endothelial cell line time  (ATCC)


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    ATCC microvascular endothelial cell line time
    Microvascular Endothelial Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    microvascular endothelial cell line time  (ATCC)


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    ATCC microvascular endothelial cell line time
    Microvascular Endothelial Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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  • 94
    ATCC human cell line time
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Human Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cell line time/product/ATCC
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    94
    ATCC time crl 4025 cell lines
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Time Crl 4025 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/time crl 4025 cell lines/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    time crl 4025 cell lines - by Bioz Stars, 2024-02
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    94
    ATCC time cell line atcc crl 4025 authentication
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Time Cell Line Atcc Crl 4025 Authentication, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/time cell line atcc crl 4025 authentication/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    time cell line atcc crl 4025 authentication - by Bioz Stars, 2024-02
    94/100 stars
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    94
    ATCC microvascular endothelial cell line time
    Human endothelial cells (cell line <t>TIME,</t> <t>ATCC</t> ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).
    Microvascular Endothelial Cell Line Time, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microvascular endothelial cell line time/product/ATCC
    Average 94 stars, based on 1 article reviews
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    94
    ATCC shakt1 stable cell lines telomerase
    (A) Representative confocal images showing immunofluorescence staining of VE-cadherin on HMEC monolayers transfected with either scrambled ShRNA or ShRNA targeting Akt1. (B) Representative Western blot images and band densitometry quantification of stable ShControl and <t>ShAkt1</t> HMEC lysates showing reduced Akt1 expression in ShAkt1 HMEC compared to ShControl HMEC (n=3). (C) Quantification of the gap area in control and Akt1 knockdown HMEC monolayers normalized to the total area (n=4). (D) Quantification of the number of gaps in control and Akt1 knockdown HMEC monolayers normalized to the number of nuclei per field (n=4). (E) Representative confocal images showing VE-cadherin staining on the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment. (F) Quantification of the number of gaps in the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment (n=4). Data are represented as mean ± SD. *P<0.01, #P<0.05, scale bar 20 μm.
    Shakt1 Stable Cell Lines Telomerase, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shakt1 stable cell lines telomerase/product/ATCC
    Average 94 stars, based on 1 article reviews
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    shakt1 stable cell lines telomerase - by Bioz Stars, 2024-02
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    Image Search Results


    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).

    Journal: Cells

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    doi: 10.3390/cells10112843

    Figure Lengend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h and compared to untreated controls. Three biological replicates were tested in each group. Transcriptome data obtained by next generation sequencing (NGS) were gathered into a gene set enrichment analysis (GSEA). Shown are 5 enrichment plots, which correspond to defined gene sets. ( A ) GO-Cellular-Response-To-Cytokine-Stimulation, ( B ) GO-Inflammatory-Response, ( C ) GO-Regulation-Of-Cytokine-Production, ( D ) GO-Homophilic-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules, ( E ) GO-Cell-Adhesion-Via-Plasma-Membrane-Adhesion-Molecules. Each plot shows the running Enrichment score (ES) for the gene set, which reflects the degree to which a gene set is over-represented at the top or bottom of a ranked list of genes. A positive ES indicates gene set enrichment at the top of the ranked list (=upregulation); a negative ES indicates gene set enrichment at the bottom of the ranked list (=downregulation).

    Article Snippet: The human cell line TIME (ATCC ® number CRL-4025) cultured at 37 °C and 5% CO 2 in a humid atmosphere served as an in vitro model for microvascular endothelial cells.

    Techniques: Next-Generation Sequencing

    The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.

    Journal: Cells

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    doi: 10.3390/cells10112843

    Figure Lengend Snippet: The cellular impedance of human endothelial cells (cell line TIME, ATCC ® number CRL-4025) was investigated in real-time using the xCELLigence ® RTCA DP instrument (ACEA Bioscience Incorporation). Changes in impedance are reported by the dimensionless parameter “cell index”. The blue and red data points symbolize cells cultured in basal cell culture medium versus medium containing 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for a defined time, respectively. The arrows indicate the time points of the performed medium changes. Three biological replicates and two technical replicates were tested in each group. Data are shown as means ± standard deviation.

    Article Snippet: The human cell line TIME (ATCC ® number CRL-4025) cultured at 37 °C and 5% CO 2 in a humid atmosphere served as an in vitro model for microvascular endothelial cells.

    Techniques: Cell Culture, Standard Deviation

    Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.

    Journal: Cells

    Article Title: Loss of Endothelial Barrier Function in the Inflammatory Setting: Indication for a Cytokine-Mediated Post-Transcriptional Mechanism by Virtue of Upregulation of miRNAs miR-29a-3p, miR-29b-3p, and miR-155-5p

    doi: 10.3390/cells10112843

    Figure Lengend Snippet: Human endothelial cells (cell line TIME, ATCC ® number CRL-4025) were evaluated following stimulation with 5 ng/mL each of IL-1β, TNF-α, and IFN-γ for 24 h, and compared to unstimulated controls. Absolute quantification of miRNAs from isolated total RNA was performed using ddPCR analysis. The QX 200 Droplet Digital PCR System (Bio-Rad) and specific LNA PCR primers (Qiagen) were used according to the manufacturer’s instructions. Based on the number of measured positive droplets, the system calculates the copy number of the target RNA in the total mixture, assuming a Poisson distribution. Since a defined amount of RNA sample is used in each ddPCR assay, the copy number per ng RNA can be determined. The box plots depict the median of determined miRNA expression levels, the lower and upper quantile, and the two extreme values. Each group included six biological replicates and two technical replicates. An unpaired t-test was used to identify significant differences. Statistical analysis was performed utilizing GraphPad Prism 9 (GaphPad Software, La Jolla, CA, USA). In all cases, p < 0.05 was assumed to indicate significant differences.

    Article Snippet: The human cell line TIME (ATCC ® number CRL-4025) cultured at 37 °C and 5% CO 2 in a humid atmosphere served as an in vitro model for microvascular endothelial cells.

    Techniques: Isolation, Digital PCR, Expressing, Software

    (A) Representative confocal images showing immunofluorescence staining of VE-cadherin on HMEC monolayers transfected with either scrambled ShRNA or ShRNA targeting Akt1. (B) Representative Western blot images and band densitometry quantification of stable ShControl and ShAkt1 HMEC lysates showing reduced Akt1 expression in ShAkt1 HMEC compared to ShControl HMEC (n=3). (C) Quantification of the gap area in control and Akt1 knockdown HMEC monolayers normalized to the total area (n=4). (D) Quantification of the number of gaps in control and Akt1 knockdown HMEC monolayers normalized to the number of nuclei per field (n=4). (E) Representative confocal images showing VE-cadherin staining on the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment. (F) Quantification of the number of gaps in the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment (n=4). Data are represented as mean ± SD. *P<0.01, #P<0.05, scale bar 20 μm.

    Journal: Journal of cellular physiology

    Article Title: Modulation of long-term endothelial-barrier integrity is conditional to the cross-talk between Akt and Src signaling

    doi: 10.1002/jcp.25791

    Figure Lengend Snippet: (A) Representative confocal images showing immunofluorescence staining of VE-cadherin on HMEC monolayers transfected with either scrambled ShRNA or ShRNA targeting Akt1. (B) Representative Western blot images and band densitometry quantification of stable ShControl and ShAkt1 HMEC lysates showing reduced Akt1 expression in ShAkt1 HMEC compared to ShControl HMEC (n=3). (C) Quantification of the gap area in control and Akt1 knockdown HMEC monolayers normalized to the total area (n=4). (D) Quantification of the number of gaps in control and Akt1 knockdown HMEC monolayers normalized to the number of nuclei per field (n=4). (E) Representative confocal images showing VE-cadherin staining on the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment. (F) Quantification of the number of gaps in the vehicle (DMSO), Src inhibitor (PP2) and Akt inhibitor (TCBN) treated HMEC monolayers 24 hours after treatment (n=4). Data are represented as mean ± SD. *P<0.01, #P<0.05, scale bar 20 μm.

    Article Snippet: Cell culture and preparation of ShAkt1 stable cell lines Telomerase-immortalized HMECs (CRL-4025; ATCC, Manassas, VA) were maintained in EBM-2 with a Growth factor-2 Bullet Kit (Lonza; Walkersville, MD).

    Techniques: Immunofluorescence, Staining, Transfection, shRNA, Western Blot, Expressing

    (A–B) Representative Western blot images and corresponding bar graph of Akt Ser473 phosphorylation and total-Akt in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (C–D) Representative Western blot images and corresponding bar graph of Src Tyr416 phosphorylation and total-Src in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (E–F) Real-time changes and a bar graph showing the quantification of changes in the ShControl and ShAkt1 HMEC-barrier resistance upon treatment with either vehicle (PBS) or 5 ng/ml TGFβ1 as measured using ECIS equipment. Data are represented as mean ± SD. (n=3), *p<0.01.

    Journal: Journal of cellular physiology

    Article Title: Modulation of long-term endothelial-barrier integrity is conditional to the cross-talk between Akt and Src signaling

    doi: 10.1002/jcp.25791

    Figure Lengend Snippet: (A–B) Representative Western blot images and corresponding bar graph of Akt Ser473 phosphorylation and total-Akt in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (C–D) Representative Western blot images and corresponding bar graph of Src Tyr416 phosphorylation and total-Src in HMEC lysates treated in the presence and absence of 5 ng/ml TGFβ1 for 1–24 hours, respectively. (E–F) Real-time changes and a bar graph showing the quantification of changes in the ShControl and ShAkt1 HMEC-barrier resistance upon treatment with either vehicle (PBS) or 5 ng/ml TGFβ1 as measured using ECIS equipment. Data are represented as mean ± SD. (n=3), *p<0.01.

    Article Snippet: Cell culture and preparation of ShAkt1 stable cell lines Telomerase-immortalized HMECs (CRL-4025; ATCC, Manassas, VA) were maintained in EBM-2 with a Growth factor-2 Bullet Kit (Lonza; Walkersville, MD).

    Techniques: Western Blot