Journal: Materials Today Bio

Article Title: Polyelectrolyte nanoparticles enable intracellular delivery of STING protein fragments for ovarian cancer immunotherapy

doi: 10.1016/j.mtbio.2026.103251

Figure Lengend Snippet: Polyanion component of nanoparticle allows for tuning cell specificity in STING activation. ( A ) Chemical structures of polyanions examined in nanoparticle formulations. ( B ) Z-Average diameter and polydispersity index (N = 8-13 independent formulations) as well as ( C ) zeta potential (N = 8-10 independent formulations) measurements of nanoparticles. (D) Fold-change in STING signaling activation compared to buffer 24 h after treatment with nanoparticle formulations at STINGΔTM doses of 5 μg/mL (RAW264.7), 8 μg/mL (HEK293T), 10 μg/mL (THP1), or 20 μg/mL (BPPNM and SKOV3). STING signaling measured as luminescent IRF3 reporter for RAW264.7, THP1, and HEK293T reporters or chemokine CXCL10 secretion in supernatant for SKOV3 and BPPNM. Each row represents one of N = 3 biological replicates, displaying the geometric mean of N = 3 technical replicates measuring the fold change in STING activation compared to the buffer control. Data represented as geometric mean ± standard deviation for data on log-scale and mean ± standard deviation for data on linear scale.

Article Snippet: RAW-Lucia ISG (rawl-isg), THP1-Dual (thpd-nfis), and HEK-Blue mTLR4 (hkb-mtlr4) reporter cells were purchased from InvivoGen.

Techniques: Activation Assay, Zeta Potential Analyzer, Control, Standard Deviation