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thle 2 liver epithelial cells  (ATCC)


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    Structured Review

    ATCC thle 2 liver epithelial cells
    Thle 2 Liver Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thle+2/THLE-2/pm42885052-161-4-8
    Average 98 stars, based on 663 article reviews
    thle 2 liver epithelial cells - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    In Vitro:

    Article Title: Development of Type II Glucose Transporter Inhibitors: Phloretin as a GLUT-2 Screening Template from In Silico Modeling to In Vitro Assessment
    Article Snippet: .. HepG2 (human HCC; ATCC HB-8065; ATCC, Manassas, VA, USA) and THLE-2 (human immortalized normal hepatocyte; ATCC CRL-2706) cell lines were used as in vitro models for HCC and normal liver cells, respectively, and maintained under standard culture conditions. .. Briefly, HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 11995-065) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. A5209401) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. 15140-122).

    Article Title: KCMF1 promotes MASLD progression via K48-linked ubiquitination and degradation of AMPKα.
    Article Snippet: Background/Aims: Metabolic dysfunction-associated steatotic liver disease (MASLD) lacks effective pharmacotherapy.. Although AMP-activated protein kinase (AMPK) is a central metabolic regulator with hepatoprotective effects, the upstream mechanisms governing its degradation in MASLD remain poorly defined.. Here, we identify potassium channel modulatory factor 1 (KCMF1) as a previously unrecognized E3 ubiquitin ligase targeting AMPKα and investigate its role in MASLD pathogenesis.

    Cell Culture:

    Article Title: KCMF1 promotes MASLD progression via K48-linked ubiquitination and degradation of AMPKα.
    Article Snippet: Background/Aims: Metabolic dysfunction-associated steatotic liver disease (MASLD) lacks effective pharmacotherapy.. Although AMP-activated protein kinase (AMPK) is a central metabolic regulator with hepatoprotective effects, the upstream mechanisms governing its degradation in MASLD remain poorly defined.. Here, we identify potassium channel modulatory factor 1 (KCMF1) as a previously unrecognized E3 ubiquitin ligase targeting AMPKα and investigate its role in MASLD pathogenesis.

    Transfection:

    Article Title: KCMF1 promotes MASLD progression via K48-linked ubiquitination and degradation of AMPKα.
    Article Snippet: Background/Aims: Metabolic dysfunction-associated steatotic liver disease (MASLD) lacks effective pharmacotherapy.. Although AMP-activated protein kinase (AMPK) is a central metabolic regulator with hepatoprotective effects, the upstream mechanisms governing its degradation in MASLD remain poorly defined.. Here, we identify potassium channel modulatory factor 1 (KCMF1) as a previously unrecognized E3 ubiquitin ligase targeting AMPKα and investigate its role in MASLD pathogenesis.

    other:

    Article Title: Phospho-JNK agonists show promising effects for the treatment of hepatocellular carcinoma
    Article Snippet: THLE-2 , ATCC , ATCC-CRL-2706.

    Vaccines:

    Article Title: Phytochemical-mediated green synthesis of selenium nanoparticles using Catharanthus roseus and their physicochemical characterization, biological evaluation, and molecular docking analysis
    Article Snippet: .. THLE-2 and HepG2 cells were purchased from the Egyptian Holding Company for Vaccines and Sera (VACSERA) via the American Type Culture Collection (ATCC). ..



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    ATCC normal thle 2 liver cells
    In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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    In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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    ATCC human normal liver cell line thle 2
    In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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    ATCC human normal liver epithelial cells
    In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
    Human Normal Liver Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thle 2  (ATCC)
    98
    ATCC thle 2
    In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
    Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of normal THLE-2 cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).

    Journal: International Journal of Nanomedicine

    Article Title: Sequential-Delivery Gel Microspheres Integrating HAase-Mediated ECM Remodeling and GA-Active Targeting for Deep Tumor Penetration in TACE-Treated HCC

    doi: 10.2147/IJN.S634549

    Figure Lengend Snippet: In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of normal THLE-2 cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).

    Article Snippet: Huh-7 hepatocellular carcinoma cells (BNCC337690) and normal THLE-2 liver cells (CRL-2638) were purchased from BeNa Culture Collection and ATCC, respectively.

    Techniques: In Vitro Evaluation, Flow Cytometry, Analysis, Incubation, Fluorescence, Derivative Assay