Structured Review

Millipore thioflavin s solution
IL-1Ra immunoreactivity is detectable in human islet α-cells. (A)  Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green).  (B)  Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).
Thioflavin S Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97/100 stars

Images

1) Product Images from "Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets"

Article Title: Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets

Journal: Molecular Metabolism

doi: 10.1016/j.molmet.2017.05.016

IL-1Ra immunoreactivity is detectable in human islet α-cells. (A)  Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green).  (B)  Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).
Figure Legend Snippet: IL-1Ra immunoreactivity is detectable in human islet α-cells. (A) Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green). (B) Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).

Techniques Used: Transduction, Cell Culture, Immunolabeling, Triple Immunostaining

IL-1Ra levels are increased in oligomer-positive islet areas and reduced in thioflavin S-positive islet areas. (A) Paraffin embedded sections from 7-day cultured (11.1 mmol/L glucose) non-transduced or transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) islets were immunolabeled for insulin (red) and IL-1Ra (green). The squares (dashed white lines) denote enlarged regions shown as inserts. (B) Human islets were immunolabeled for IL-1Ra (green) and A11 (red; top panel) or insulin (red), IL-1Ra (green) and thioflavin S (Thio S; blue; bottom panel). (C) Paraffin-embedded sections from (left to right): ob/ob mouse and human adipose tissue immunolabeled for IL-1Ra (green; positive control); human islets incubated with secondary antibody alone (negative control) and anakinra-treated human islets immunolabeled for insulin (red) and IL-1Ra (green). Scale bar = 50 μm; inserts: ×3 (A11: ×4). Micrographs are representative of four independent studies (4 human islet preparations).
Figure Legend Snippet: IL-1Ra levels are increased in oligomer-positive islet areas and reduced in thioflavin S-positive islet areas. (A) Paraffin embedded sections from 7-day cultured (11.1 mmol/L glucose) non-transduced or transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) islets were immunolabeled for insulin (red) and IL-1Ra (green). The squares (dashed white lines) denote enlarged regions shown as inserts. (B) Human islets were immunolabeled for IL-1Ra (green) and A11 (red; top panel) or insulin (red), IL-1Ra (green) and thioflavin S (Thio S; blue; bottom panel). (C) Paraffin-embedded sections from (left to right): ob/ob mouse and human adipose tissue immunolabeled for IL-1Ra (green; positive control); human islets incubated with secondary antibody alone (negative control) and anakinra-treated human islets immunolabeled for insulin (red) and IL-1Ra (green). Scale bar = 50 μm; inserts: ×3 (A11: ×4). Micrographs are representative of four independent studies (4 human islet preparations).

Techniques Used: Cell Culture, Transduction, Immunolabeling, Positive Control, Incubation, Negative Control

Treatment with neutralizing IL-1β antibody markedly reduces islet IL-1β immunoreactivity and prevents β-cell Fas upregulation induced by amyloid formation in cultured human islets. Human islets non-transduced or transduced with Ad-prohIAPP-siRNA or Ad-control-siRNA (MOI: 20) were cultured with (+nIL1β) or without (-nIL1β) a neutralizing monoclonal human IL-1β antibody (1 μg/ml) in CMRL (11.1 mmol/L glucose) for 7 days. (A) Paraffin-embedded islet sections were immunolabeled for insulin (red), IL-1β or Fas (green) and thioflavin S (Thio S; blue) as indicated. The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of each image. The top right inserts in each micrograph show immunolabeling for insulin (green) and A11 (red). Scale bar = 50 μm; inserts: ×3 (A11: ×4). (B) The proportion of Fas positive islet β-cells (fold over day 0). The percentage of (C) A11 (oligomer)-positive islets, (D) thioflavin S (amyloid) positive islets, and (E) amyloid area to total islet area. Data are presented as mean ± SEM of four independent studies (4 human islet preparations); n = 15–20 islets per condition in each study. *vs day 0; #vs corresponding non-treated group ( p
Figure Legend Snippet: Treatment with neutralizing IL-1β antibody markedly reduces islet IL-1β immunoreactivity and prevents β-cell Fas upregulation induced by amyloid formation in cultured human islets. Human islets non-transduced or transduced with Ad-prohIAPP-siRNA or Ad-control-siRNA (MOI: 20) were cultured with (+nIL1β) or without (-nIL1β) a neutralizing monoclonal human IL-1β antibody (1 μg/ml) in CMRL (11.1 mmol/L glucose) for 7 days. (A) Paraffin-embedded islet sections were immunolabeled for insulin (red), IL-1β or Fas (green) and thioflavin S (Thio S; blue) as indicated. The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of each image. The top right inserts in each micrograph show immunolabeling for insulin (green) and A11 (red). Scale bar = 50 μm; inserts: ×3 (A11: ×4). (B) The proportion of Fas positive islet β-cells (fold over day 0). The percentage of (C) A11 (oligomer)-positive islets, (D) thioflavin S (amyloid) positive islets, and (E) amyloid area to total islet area. Data are presented as mean ± SEM of four independent studies (4 human islet preparations); n = 15–20 islets per condition in each study. *vs day 0; #vs corresponding non-treated group ( p

Techniques Used: Cell Culture, Transduction, Immunolabeling

2) Product Images from "A novel technique for simultaneous bilateral brain infusions in a mouse model of neurodegenerative disease"

Article Title: A novel technique for simultaneous bilateral brain infusions in a mouse model of neurodegenerative disease

Journal: Journal of neuroscience methods

doi: 10.1016/j.jneumeth.2009.08.021

(A) Thioflavin S staining on 25 μm coronal sections (montages) of 3 different PS/APP mice at 10 months of age showing plaque variability in the hippocampus and neighboring cortex. (B) Scatter-plot of plaque load data from 5 brain sections through 6 ten-month-old PS/APP mice comparing standard deviation (plaque variance) between total hemispheres of the same animal (28%), between sides of the same section (11%) and between animals (78%). All of the data from the left hemisphere are compared with the right for each mouse (Hemi–Hemi, 1 data point for each mouse); the left half of a single slice is compared with the right side (Left–Right, 1 data point for each slice); and a permuted comparison of each mouse with every other mouse (Mouse–Mouse, 1 data point for each pair of mice). Where data were aggregated (Hemi–Hemi and Mouse–Mouse), the mean of the aggregated counts was used to make the standard deviation comparable to the single slice data (standard deviation of two numbers).
Figure Legend Snippet: (A) Thioflavin S staining on 25 μm coronal sections (montages) of 3 different PS/APP mice at 10 months of age showing plaque variability in the hippocampus and neighboring cortex. (B) Scatter-plot of plaque load data from 5 brain sections through 6 ten-month-old PS/APP mice comparing standard deviation (plaque variance) between total hemispheres of the same animal (28%), between sides of the same section (11%) and between animals (78%). All of the data from the left hemisphere are compared with the right for each mouse (Hemi–Hemi, 1 data point for each mouse); the left half of a single slice is compared with the right side (Left–Right, 1 data point for each slice); and a permuted comparison of each mouse with every other mouse (Mouse–Mouse, 1 data point for each pair of mice). Where data were aggregated (Hemi–Hemi and Mouse–Mouse), the mean of the aggregated counts was used to make the standard deviation comparable to the single slice data (standard deviation of two numbers).

Techniques Used: Staining, Mouse Assay, Standard Deviation

3) Product Images from "Tau Pathology Profile Across a Parietal-Hippocampal Brain Network Is Associated With Spatial Reorientation Learning and Memory Performance in the 3xTg-AD Mouse"

Article Title: Tau Pathology Profile Across a Parietal-Hippocampal Brain Network Is Associated With Spatial Reorientation Learning and Memory Performance in the 3xTg-AD Mouse

Journal: Frontiers in aging

doi: 10.3389/fragi.2021.655015

Plaques were absent from most brain regions for all groups except 12-month male 3xTg-AD mice. Top . Representative image of Thioflavin S staining for a 6-month female 3xTg-AD mouse with no plaques. Bottom. Representative image of Thioflavin S staining for a 12-month male 3xTg-AD mouse with plaques (green) in dorsal subiculum (dSub).
Figure Legend Snippet: Plaques were absent from most brain regions for all groups except 12-month male 3xTg-AD mice. Top . Representative image of Thioflavin S staining for a 6-month female 3xTg-AD mouse with no plaques. Bottom. Representative image of Thioflavin S staining for a 12-month male 3xTg-AD mouse with plaques (green) in dorsal subiculum (dSub).

Techniques Used: Mouse Assay, Staining

4) Product Images from "Suppression of Autophagy and Activation of Glycogen Synthase Kinase 3beta Facilitate the Aggregate Formation of Tau"

Article Title: Suppression of Autophagy and Activation of Glycogen Synthase Kinase 3beta Facilitate the Aggregate Formation of Tau

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

doi: 10.4196/kjpp.2011.15.2.107

Activ me GSK3β and 3-MA treatment result in the formation of thioflavin-S-positive inclusions of tau in a co-operative manner. Each tau and active GSK3β or enzyme-inactive GSK3β constructs were transiently co-transfected into CHO
Figure Legend Snippet: Activ me GSK3β and 3-MA treatment result in the formation of thioflavin-S-positive inclusions of tau in a co-operative manner. Each tau and active GSK3β or enzyme-inactive GSK3β constructs were transiently co-transfected into CHO

Techniques Used: Construct, Transfection

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    Millipore thioflavin s solution
    IL-1Ra immunoreactivity is detectable in human islet α-cells. (A)  Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green).  (B)  Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).
    Thioflavin S Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thioflavin s solution/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thioflavin s solution - by Bioz Stars, 2022-08
    97/100 stars
      Buy from Supplier

    99
    Millipore dye thioflavin s
    Injury Patterns on Histology. Images show TTC, <t>Thioflavin</t> S and H E stained short axis sections obtained from explanted hearts at 24 h post intervention. TTC sections indicate region on necrosis appearing white in the I-HEM group (no hemorrhage) and reddish white in I+HEM group (hemorrhage); the +HEM group did no show infarction. Under ultra-violet light, non-fluorescent regions on the Thioflavin S stain highlight areas of compromised endothelium as seen in the +HEM and I+HEM groups; this confirms presence of microvascular damage in the hemorrhage groups. The H E images were obtained from the region of interest shown on the TTC stained sections. Hemorrhage was apparent in the +HEM and I+HEM groups as evidenced from the interstitial distribution of red blood cells ( open arrow heads ) in the affected LAD territory; red blood cells were absent in the I-HEM group. Edematous development was observed in all three groups ( arrows ) along with the presence of inflammatory cells ( closed arrow heads )
    Dye Thioflavin S, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dye thioflavin s/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dye thioflavin s - by Bioz Stars, 2022-08
    99/100 stars
      Buy from Supplier

    97
    Millipore thioflavin s
    Native pancreas biopsies stained for insulin ( red ) and <t>thioflavin</t> S ( green ) are demonstrated from normal monkey (A), normal human (B), and type 2 diabetic human (C). Native pancreas biopsies stained for insulin ( red ) and IAPP ( green ) are also demonstrated from normal monkey (D), normal human (E), and type 2 diabetic human (F), where intracellular colocalization of insulin and IAPP appears orange . Only the type 2 diabetic pancreas with amyloid present by thioflavin S evidences additional IAPP present extracellularly immediately surrounding β-cells. Intrahepatic islet biopsies stained for insulin ( red ) and IAPP ( green ) similarly show intracellular colocalization of insulin and IAPP in a biopsy without amyloid (G), and intra- and extracellular IAPP in a biopsy with amyloid (H). In I, separate images for insulin ( red , 1), IAPP ( green , 2), and their combination (3) are shown.
    Thioflavin S, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thioflavin s/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thioflavin s - by Bioz Stars, 2022-08
    97/100 stars
      Buy from Supplier

    Image Search Results


    IL-1Ra immunoreactivity is detectable in human islet α-cells. (A)  Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green).  (B)  Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).

    Journal: Molecular Metabolism

    Article Title: Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets

    doi: 10.1016/j.molmet.2017.05.016

    Figure Lengend Snippet: IL-1Ra immunoreactivity is detectable in human islet α-cells. (A) Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green). (B) Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).

    Article Snippet: For thioflavin S staining, sections were incubated with 0.5% (wt/vol.) thioflavin S solution (Sigma–Aldrich, Oakville, ON, Canada) for 5 min at room temperature.

    Techniques: Transduction, Cell Culture, Immunolabeling, Triple Immunostaining

    IL-1Ra levels are increased in oligomer-positive islet areas and reduced in thioflavin S-positive islet areas. (A) Paraffin embedded sections from 7-day cultured (11.1 mmol/L glucose) non-transduced or transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) islets were immunolabeled for insulin (red) and IL-1Ra (green). The squares (dashed white lines) denote enlarged regions shown as inserts. (B) Human islets were immunolabeled for IL-1Ra (green) and A11 (red; top panel) or insulin (red), IL-1Ra (green) and thioflavin S (Thio S; blue; bottom panel). (C) Paraffin-embedded sections from (left to right): ob/ob mouse and human adipose tissue immunolabeled for IL-1Ra (green; positive control); human islets incubated with secondary antibody alone (negative control) and anakinra-treated human islets immunolabeled for insulin (red) and IL-1Ra (green). Scale bar = 50 μm; inserts: ×3 (A11: ×4). Micrographs are representative of four independent studies (4 human islet preparations).

    Journal: Molecular Metabolism

    Article Title: Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets

    doi: 10.1016/j.molmet.2017.05.016

    Figure Lengend Snippet: IL-1Ra levels are increased in oligomer-positive islet areas and reduced in thioflavin S-positive islet areas. (A) Paraffin embedded sections from 7-day cultured (11.1 mmol/L glucose) non-transduced or transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) islets were immunolabeled for insulin (red) and IL-1Ra (green). The squares (dashed white lines) denote enlarged regions shown as inserts. (B) Human islets were immunolabeled for IL-1Ra (green) and A11 (red; top panel) or insulin (red), IL-1Ra (green) and thioflavin S (Thio S; blue; bottom panel). (C) Paraffin-embedded sections from (left to right): ob/ob mouse and human adipose tissue immunolabeled for IL-1Ra (green; positive control); human islets incubated with secondary antibody alone (negative control) and anakinra-treated human islets immunolabeled for insulin (red) and IL-1Ra (green). Scale bar = 50 μm; inserts: ×3 (A11: ×4). Micrographs are representative of four independent studies (4 human islet preparations).

    Article Snippet: For thioflavin S staining, sections were incubated with 0.5% (wt/vol.) thioflavin S solution (Sigma–Aldrich, Oakville, ON, Canada) for 5 min at room temperature.

    Techniques: Cell Culture, Transduction, Immunolabeling, Positive Control, Incubation, Negative Control

    Treatment with neutralizing IL-1β antibody markedly reduces islet IL-1β immunoreactivity and prevents β-cell Fas upregulation induced by amyloid formation in cultured human islets. Human islets non-transduced or transduced with Ad-prohIAPP-siRNA or Ad-control-siRNA (MOI: 20) were cultured with (+nIL1β) or without (-nIL1β) a neutralizing monoclonal human IL-1β antibody (1 μg/ml) in CMRL (11.1 mmol/L glucose) for 7 days. (A) Paraffin-embedded islet sections were immunolabeled for insulin (red), IL-1β or Fas (green) and thioflavin S (Thio S; blue) as indicated. The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of each image. The top right inserts in each micrograph show immunolabeling for insulin (green) and A11 (red). Scale bar = 50 μm; inserts: ×3 (A11: ×4). (B) The proportion of Fas positive islet β-cells (fold over day 0). The percentage of (C) A11 (oligomer)-positive islets, (D) thioflavin S (amyloid) positive islets, and (E) amyloid area to total islet area. Data are presented as mean ± SEM of four independent studies (4 human islet preparations); n = 15–20 islets per condition in each study. *vs day 0; #vs corresponding non-treated group ( p

    Journal: Molecular Metabolism

    Article Title: Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets

    doi: 10.1016/j.molmet.2017.05.016

    Figure Lengend Snippet: Treatment with neutralizing IL-1β antibody markedly reduces islet IL-1β immunoreactivity and prevents β-cell Fas upregulation induced by amyloid formation in cultured human islets. Human islets non-transduced or transduced with Ad-prohIAPP-siRNA or Ad-control-siRNA (MOI: 20) were cultured with (+nIL1β) or without (-nIL1β) a neutralizing monoclonal human IL-1β antibody (1 μg/ml) in CMRL (11.1 mmol/L glucose) for 7 days. (A) Paraffin-embedded islet sections were immunolabeled for insulin (red), IL-1β or Fas (green) and thioflavin S (Thio S; blue) as indicated. The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of each image. The top right inserts in each micrograph show immunolabeling for insulin (green) and A11 (red). Scale bar = 50 μm; inserts: ×3 (A11: ×4). (B) The proportion of Fas positive islet β-cells (fold over day 0). The percentage of (C) A11 (oligomer)-positive islets, (D) thioflavin S (amyloid) positive islets, and (E) amyloid area to total islet area. Data are presented as mean ± SEM of four independent studies (4 human islet preparations); n = 15–20 islets per condition in each study. *vs day 0; #vs corresponding non-treated group ( p

    Article Snippet: For thioflavin S staining, sections were incubated with 0.5% (wt/vol.) thioflavin S solution (Sigma–Aldrich, Oakville, ON, Canada) for 5 min at room temperature.

    Techniques: Cell Culture, Transduction, Immunolabeling

    Injury Patterns on Histology. Images show TTC, Thioflavin S and H E stained short axis sections obtained from explanted hearts at 24 h post intervention. TTC sections indicate region on necrosis appearing white in the I-HEM group (no hemorrhage) and reddish white in I+HEM group (hemorrhage); the +HEM group did no show infarction. Under ultra-violet light, non-fluorescent regions on the Thioflavin S stain highlight areas of compromised endothelium as seen in the +HEM and I+HEM groups; this confirms presence of microvascular damage in the hemorrhage groups. The H E images were obtained from the region of interest shown on the TTC stained sections. Hemorrhage was apparent in the +HEM and I+HEM groups as evidenced from the interstitial distribution of red blood cells ( open arrow heads ) in the affected LAD territory; red blood cells were absent in the I-HEM group. Edematous development was observed in all three groups ( arrows ) along with the presence of inflammatory cells ( closed arrow heads )

    Journal: Journal of Cardiovascular Magnetic Resonance

    Article Title: Hemorrhage promotes inflammation and myocardial damage following acute myocardial infarction: insights from a novel preclinical model and cardiovascular magnetic resonance

    doi: 10.1186/s12968-017-0361-7

    Figure Lengend Snippet: Injury Patterns on Histology. Images show TTC, Thioflavin S and H E stained short axis sections obtained from explanted hearts at 24 h post intervention. TTC sections indicate region on necrosis appearing white in the I-HEM group (no hemorrhage) and reddish white in I+HEM group (hemorrhage); the +HEM group did no show infarction. Under ultra-violet light, non-fluorescent regions on the Thioflavin S stain highlight areas of compromised endothelium as seen in the +HEM and I+HEM groups; this confirms presence of microvascular damage in the hemorrhage groups. The H E images were obtained from the region of interest shown on the TTC stained sections. Hemorrhage was apparent in the +HEM and I+HEM groups as evidenced from the interstitial distribution of red blood cells ( open arrow heads ) in the affected LAD territory; red blood cells were absent in the I-HEM group. Edematous development was observed in all three groups ( arrows ) along with the presence of inflammatory cells ( closed arrow heads )

    Article Snippet: Prior to sacrifice, a 4% solution of fluorescent dye thioflavin S (1 ml/kg, Sigma-Aldrich) was intravenously injected as a bolus to examine endothelial cell distribution and arterial patency in the heart [ ].

    Techniques: Staining

    Native pancreas biopsies stained for insulin ( red ) and thioflavin S ( green ) are demonstrated from normal monkey (A), normal human (B), and type 2 diabetic human (C). Native pancreas biopsies stained for insulin ( red ) and IAPP ( green ) are also demonstrated from normal monkey (D), normal human (E), and type 2 diabetic human (F), where intracellular colocalization of insulin and IAPP appears orange . Only the type 2 diabetic pancreas with amyloid present by thioflavin S evidences additional IAPP present extracellularly immediately surrounding β-cells. Intrahepatic islet biopsies stained for insulin ( red ) and IAPP ( green ) similarly show intracellular colocalization of insulin and IAPP in a biopsy without amyloid (G), and intra- and extracellular IAPP in a biopsy with amyloid (H). In I, separate images for insulin ( red , 1), IAPP ( green , 2), and their combination (3) are shown.

    Journal: Endocrinology

    Article Title: Accumulation of Intrahepatic Islet Amyloid in a Nonhuman Primate Transplant Model

    doi: 10.1210/en.2011-1560

    Figure Lengend Snippet: Native pancreas biopsies stained for insulin ( red ) and thioflavin S ( green ) are demonstrated from normal monkey (A), normal human (B), and type 2 diabetic human (C). Native pancreas biopsies stained for insulin ( red ) and IAPP ( green ) are also demonstrated from normal monkey (D), normal human (E), and type 2 diabetic human (F), where intracellular colocalization of insulin and IAPP appears orange . Only the type 2 diabetic pancreas with amyloid present by thioflavin S evidences additional IAPP present extracellularly immediately surrounding β-cells. Intrahepatic islet biopsies stained for insulin ( red ) and IAPP ( green ) similarly show intracellular colocalization of insulin and IAPP in a biopsy without amyloid (G), and intra- and extracellular IAPP in a biopsy with amyloid (H). In I, separate images for insulin ( red , 1), IAPP ( green , 2), and their combination (3) are shown.

    Article Snippet: Islet amyloid was detected by staining with thioflavin S (5 g/1000 ml solution; Sigma-Aldrich, St. Louis, MO) as previously described ( ) using normal cynomolgus and human pancreata as negative controls and type 2 diabetic human pancreata as positive controls ( ).

    Techniques: Staining

    Intrahepatic islet transplant biopsies stained for insulin ( red ) and thioflavin S ( green ) are shown from recipient T4536 at posttransplant months 18 (A), 40 (B), and 46 (C), recipient 211050 at month 4 (D), recipient 93–100 at month 10 (E), recipient 3845 at month 43 (F), and recipient CR14 at months 14 (G), 48 (H), and 66 (I). Apparent is an increased accumulation of islet amyloid and decrease in islet β-cells when examining the same animals (A–C and G–I) or different animals (D–F) over time. Magnification, ×40. mo, Month.

    Journal: Endocrinology

    Article Title: Accumulation of Intrahepatic Islet Amyloid in a Nonhuman Primate Transplant Model

    doi: 10.1210/en.2011-1560

    Figure Lengend Snippet: Intrahepatic islet transplant biopsies stained for insulin ( red ) and thioflavin S ( green ) are shown from recipient T4536 at posttransplant months 18 (A), 40 (B), and 46 (C), recipient 211050 at month 4 (D), recipient 93–100 at month 10 (E), recipient 3845 at month 43 (F), and recipient CR14 at months 14 (G), 48 (H), and 66 (I). Apparent is an increased accumulation of islet amyloid and decrease in islet β-cells when examining the same animals (A–C and G–I) or different animals (D–F) over time. Magnification, ×40. mo, Month.

    Article Snippet: Islet amyloid was detected by staining with thioflavin S (5 g/1000 ml solution; Sigma-Aldrich, St. Louis, MO) as previously described ( ) using normal cynomolgus and human pancreata as negative controls and type 2 diabetic human pancreata as positive controls ( ).

    Techniques: Staining

    Images shown are multilayer composites created by recoloring and scaling spectrally unmixed label component planes. A, Mask overlay indicates where inForm pattern-recognition software detected islet tissue. B, Insulin segmentation mask indicates the detection of islet β-cells as green fill. C, Thioflavin S segmentation mask indicates the detection of islet amyloid as green fill, with the islet representing an intrahepatic islet affected with amyloid from monkey CR14 at 66 months after transplant. The corresponding panels D–F demonstrate the software-detected islet tissue, β-cells, and amyloid, respectively, from a normal human islet not affected with amyloid.

    Journal: Endocrinology

    Article Title: Accumulation of Intrahepatic Islet Amyloid in a Nonhuman Primate Transplant Model

    doi: 10.1210/en.2011-1560

    Figure Lengend Snippet: Images shown are multilayer composites created by recoloring and scaling spectrally unmixed label component planes. A, Mask overlay indicates where inForm pattern-recognition software detected islet tissue. B, Insulin segmentation mask indicates the detection of islet β-cells as green fill. C, Thioflavin S segmentation mask indicates the detection of islet amyloid as green fill, with the islet representing an intrahepatic islet affected with amyloid from monkey CR14 at 66 months after transplant. The corresponding panels D–F demonstrate the software-detected islet tissue, β-cells, and amyloid, respectively, from a normal human islet not affected with amyloid.

    Article Snippet: Islet amyloid was detected by staining with thioflavin S (5 g/1000 ml solution; Sigma-Aldrich, St. Louis, MO) as previously described ( ) using normal cynomolgus and human pancreata as negative controls and type 2 diabetic human pancreata as positive controls ( ).

    Techniques: Software