thin layer chromatography hptlc silica gel 60 aluminum plates  (Merck KGaA)

 
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    Merck KGaA thin layer chromatography hptlc silica gel 60 aluminum plates
    Thin Layer Chromatography Hptlc Silica Gel 60 Aluminum Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thin layer chromatography hptlc silica gel 60 aluminum plates/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thin layer chromatography hptlc silica gel 60 aluminum plates - by Bioz Stars, 2020-09
    86/100 stars

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    High Performance Thin Layer Chromatography:

    Article Title: Membrane proteins bind lipids selectively to modulate their structure and function
    Article Snippet: .. Thin Layer Chromatography HPTLC Silica gel 60 aluminum plates (Merck Millipore) were pre-developed with 1:1 (v/v) chloroform:methanol and left to air-dry overnight. .. The plates were then impregnated with an adsorbent modifier (1:1 (v/v) 1.2% boric acid in absolute ethanol-water) followed by activation at 100°C for one hour .

    Thin Layer Chromatography:

    Article Title: Membrane proteins bind lipids selectively to modulate their structure and function
    Article Snippet: .. Thin Layer Chromatography HPTLC Silica gel 60 aluminum plates (Merck Millipore) were pre-developed with 1:1 (v/v) chloroform:methanol and left to air-dry overnight. .. The plates were then impregnated with an adsorbent modifier (1:1 (v/v) 1.2% boric acid in absolute ethanol-water) followed by activation at 100°C for one hour .

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    Merck KGaA high performance tlc hptlc aluminium backed silica gel 60 plates
    <t>HPTLC-separated</t> glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica <t>gel</t> 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).
    High Performance Tlc Hptlc Aluminium Backed Silica Gel 60 Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance tlc hptlc aluminium backed silica gel 60 plates/product/Merck KGaA
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance tlc hptlc aluminium backed silica gel 60 plates - by Bioz Stars, 2020-09
    80/100 stars
      Buy from Supplier

    85
    Merck KGaA aluminium backed silica gel 60 f254 hptlc plates
    <t>HPTLC-separated</t> glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica <t>gel</t> 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).
    Aluminium Backed Silica Gel 60 F254 Hptlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aluminium backed silica gel 60 f254 hptlc plates/product/Merck KGaA
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aluminium backed silica gel 60 f254 hptlc plates - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    80
    Merck KGaA aluminium backed silica gel 60 high performance thin layer chromatography hptlc plates
    <t>HPTLC-separated</t> glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica <t>gel</t> 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).
    Aluminium Backed Silica Gel 60 High Performance Thin Layer Chromatography Hptlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aluminium backed silica gel 60 high performance thin layer chromatography hptlc plates/product/Merck KGaA
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aluminium backed silica gel 60 high performance thin layer chromatography hptlc plates - by Bioz Stars, 2020-09
    80/100 stars
      Buy from Supplier

    92
    Merck KGaA hptlc plates
    <t>HPTLC-separated</t> glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica <t>gel</t> 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).
    Hptlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hptlc plates/product/Merck KGaA
    Average 92 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    hptlc plates - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    HPTLC-separated glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica gel 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).

    Journal: Infection and Immunity

    Article Title: Receptor Structure for F1C Fimbriae of Uropathogenic Escherichia coli

    doi:

    Figure Lengend Snippet: HPTLC-separated glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica gel 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).

    Article Snippet: Briefly, pure glycolipids (Sigma) were separated for analytical purpose on high-performance TLC (HPTLC) aluminium-backed silica gel 60 plates (Merck, Darmstadt, Germany).

    Techniques: High Performance Thin Layer Chromatography, Binding Assay, Purification, Staining