thermomixer  (Eppendorf AG)

 
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    Name:
    Eppendorf ThermoMixer C
    Description:
    You need everything The Eppendorf ThermoMixer C combines excellent mixing performance with excellent temperature control to ensure complete dependable and reproducible test results Improve your assay results by mixing and incubating samples at the same time The Eppendorf ThermoMixer C convinces users with its quick and easy to exchange SmartBlocks for all common lab vessels new and improved program functions and high level of stability for every mixing frequency There is almost no limit to the variety of applications waiting for you
    Catalog Number:
    5382000023
    Price:
    None
    Category:
    Eppendorf ThermoMixer C Instruments Temperature Control and Mixing
    Buy from Supplier


    Structured Review

    Eppendorf AG thermomixer
    Response of the 17% graphite + 1% Ag@NDs electrodes after being mixed with glucose oxidase in the <t>thermomixer</t> for 30 min and 1 h for glucose concentration between 0–60 mM ( A ). Linear concentration range for t = 30 min and t = 1 h ( B ). The experimental error was calculated as the standard deviation for n = 3 measurements.
    You need everything The Eppendorf ThermoMixer C combines excellent mixing performance with excellent temperature control to ensure complete dependable and reproducible test results Improve your assay results by mixing and incubating samples at the same time The Eppendorf ThermoMixer C convinces users with its quick and easy to exchange SmartBlocks for all common lab vessels new and improved program functions and high level of stability for every mixing frequency There is almost no limit to the variety of applications waiting for you
    https://www.bioz.com/result/thermomixer/product/Eppendorf AG
    Average 99 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    thermomixer - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Customized In Situ Functionalization of Nanodiamonds with Nanoparticles for Composite Carbon-Paste Electrodes"

    Article Title: Customized In Situ Functionalization of Nanodiamonds with Nanoparticles for Composite Carbon-Paste Electrodes

    Journal: Nanomaterials

    doi: 10.3390/nano10061179

    Response of the 17% graphite + 1% Ag@NDs electrodes after being mixed with glucose oxidase in the thermomixer for 30 min and 1 h for glucose concentration between 0–60 mM ( A ). Linear concentration range for t = 30 min and t = 1 h ( B ). The experimental error was calculated as the standard deviation for n = 3 measurements.
    Figure Legend Snippet: Response of the 17% graphite + 1% Ag@NDs electrodes after being mixed with glucose oxidase in the thermomixer for 30 min and 1 h for glucose concentration between 0–60 mM ( A ). Linear concentration range for t = 30 min and t = 1 h ( B ). The experimental error was calculated as the standard deviation for n = 3 measurements.

    Techniques Used: Concentration Assay, Standard Deviation

    2) Product Images from "Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells"

    Article Title: Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells

    Journal: Scientific Reports

    doi: 10.1038/srep36341

    ( A , B ) Adsorption isotherms of DNA on sepiolite in the presence of various polyvalent cations. Each point has error bars for 3 different experiments. Reaction conditions: 10 mM Tris-HCl pH = 7.5 and a sepiolite concentration of 1 mg/ml. ( C ) The effect of the presence of cations with different valences on DNA adsorption. Reaction conditions: 10 mM Tris-HCl pH = 7.5, salmon sperm DNA and sepiolite concentrations of 615 ng·μl −1 and 1 mg/ml, respectively. ( D , E ) Adsorption isotherms of different DNA conformations on sepiolite. Reaction conditions: 10 mM Tris-HCl pH = 7.5, 5 mM MgCl 2 , sepiolite concentration of 1 mg/ml; 50 μg of sepiolite was used in each experiment. Adsorption occurred at 25 °C for 24 hours under agitation at 700 rpm using an Eppendorf Thermomixer.
    Figure Legend Snippet: ( A , B ) Adsorption isotherms of DNA on sepiolite in the presence of various polyvalent cations. Each point has error bars for 3 different experiments. Reaction conditions: 10 mM Tris-HCl pH = 7.5 and a sepiolite concentration of 1 mg/ml. ( C ) The effect of the presence of cations with different valences on DNA adsorption. Reaction conditions: 10 mM Tris-HCl pH = 7.5, salmon sperm DNA and sepiolite concentrations of 615 ng·μl −1 and 1 mg/ml, respectively. ( D , E ) Adsorption isotherms of different DNA conformations on sepiolite. Reaction conditions: 10 mM Tris-HCl pH = 7.5, 5 mM MgCl 2 , sepiolite concentration of 1 mg/ml; 50 μg of sepiolite was used in each experiment. Adsorption occurred at 25 °C for 24 hours under agitation at 700 rpm using an Eppendorf Thermomixer.

    Techniques Used: Adsorption, Concentration Assay

    3) Product Images from "Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells"

    Article Title: Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells

    Journal: Scientific Reports

    doi: 10.1038/srep36341

    ( A , B ) Adsorption isotherms of DNA on sepiolite in the presence of various polyvalent cations. Each point has error bars for 3 different experiments. Reaction conditions: 10 mM Tris-HCl pH = 7.5 and a sepiolite concentration of 1 mg/ml. ( C ) The effect of the presence of cations with different valences on DNA adsorption. Reaction conditions: 10 mM Tris-HCl pH = 7.5, salmon sperm DNA and sepiolite concentrations of 615 ng·μl −1 and 1 mg/ml, respectively. ( D , E ) Adsorption isotherms of different DNA conformations on sepiolite. Reaction conditions: 10 mM Tris-HCl pH = 7.5, 5 mM MgCl 2 , sepiolite concentration of 1 mg/ml; 50 μg of sepiolite was used in each experiment. Adsorption occurred at 25 °C for 24 hours under agitation at 700 rpm using an Eppendorf Thermomixer.
    Figure Legend Snippet: ( A , B ) Adsorption isotherms of DNA on sepiolite in the presence of various polyvalent cations. Each point has error bars for 3 different experiments. Reaction conditions: 10 mM Tris-HCl pH = 7.5 and a sepiolite concentration of 1 mg/ml. ( C ) The effect of the presence of cations with different valences on DNA adsorption. Reaction conditions: 10 mM Tris-HCl pH = 7.5, salmon sperm DNA and sepiolite concentrations of 615 ng·μl −1 and 1 mg/ml, respectively. ( D , E ) Adsorption isotherms of different DNA conformations on sepiolite. Reaction conditions: 10 mM Tris-HCl pH = 7.5, 5 mM MgCl 2 , sepiolite concentration of 1 mg/ml; 50 μg of sepiolite was used in each experiment. Adsorption occurred at 25 °C for 24 hours under agitation at 700 rpm using an Eppendorf Thermomixer.

    Techniques Used: Adsorption, Concentration Assay

    Related Articles

    Concentration Assay:

    Article Title: Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells
    Article Snippet: .. A second set of samples was prepared similarly but incorporating DNA (commercial salmon sperm DNA, Sigma) with a mean final concentration of 650 ng·μl−1 , followed by stirring overnight at 25 °C and 700 rpm using an Eppendorf Thermomixer. ..

    Article Title: High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4
    Article Snippet: .. 0.5 μl of 50 mM of the β-galactosidase substrate (4-MUGal in DMSO, final concentration 0.80 mM) was added to all wells and the plate was incubated at 37°C with shaking at 1,400 rpm for 30 min in a ThermoMixer C instrument (Eppendorf). ..

    Incubation:

    Article Title: Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells
    Article Snippet: .. After 2 h of incubation at 25 °C and stirring at 700 rpm in an Eppendorf Thermomixer, 5.83 ml of cellular medium (MEM) was added and gently homogenized. ..

    Article Title: High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4
    Article Snippet: .. Incubation and Detection Phases The screening plate was incubated at 25°C with shaking at 1,400 rpm for 5 h (ThermoMixer C, Eppendorf). .. The optical density (OD600 ) of the bacterial culture was then measured using an Infinite M1000Pro plate reader (Tecan, CH).

    Article Title: A Unique Cyanide Adduct in Human Serum Albumin - Potential as a Surrogate Exposure Marker
    Article Snippet: .. The mixture is then incubated at 50°C for 3 h with gentle mixing (500 rpm) in an Eppendorf Thermomixer. .. At the end of the incubation, the original solution usually remains liquid, but may occasionally form a jelly-like solid.

    Article Title: High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4
    Article Snippet: .. 0.5 μl of 50 mM of the β-galactosidase substrate (4-MUGal in DMSO, final concentration 0.80 mM) was added to all wells and the plate was incubated at 37°C with shaking at 1,400 rpm for 30 min in a ThermoMixer C instrument (Eppendorf). ..

    other:

    Article Title: High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4
    Article Snippet: Use of a Thermomixer C instrument enables the required control via its platform and lid heating systems.

    Recombinant:

    Article Title: Chlamydomonas FAP265 is a tubulin polymerization promoting protein, essential for flagellar reassembly and hatching of daughter cells from the sporangium
    Article Snippet: .. FAP265 recombinant protein was eluted from the crushed gel by mechanical shaking overnight at 800 RPM on a vortex mixer (Eppendorf Thermomixer-C). .. Further, specificity of the recombinant FAP265 was determined by western blotting using antibodies against 6x-Histidine tag.

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  • 99
    Eppendorf AG thermomixer c instrument
    Effect of temperature on AHK4 signaling. Signal to bacterial density (RFU to OD 600 ) ratios, and bacterial densities, obtained following incubation (for indicated times) of E. coli KMI001-AHK4 at 25 and 29°C in M9-505 medium supplemented with 1 μM tZ. 200 μl portions of KMI001-AHK4 detection culture were dispensed into two 96-well plates with tZ. One was cultivated at 25°C, and the other at 29°C, for 5 h with shaking at 800 rpm in a <t>ThermoMixer</t> C (Eppendorf). Every 30 min. three 30 μl samples were taken from both sets of E. coli suspensions, their optical density was measured then 0.3 μl of 50 mM 4-MUGal was added and incubation continued in a 384-well plate at 37°C with shaking at 1,400 rpm for 30 min. Then the reaction was stopped by adding 9 μl of 1.2 M sodium carbonate and fluorescence was measured. (A) The RFU/OD 600 ratios clearly show that detection culture cultivated at 29°C was much less sensitive than otherwise identically cultivated culture at 25°C. (B) Growth curves of detection cultures follow the classical trend, but in contrast to expectations the higher densities of E. coli cultivated at 29°C are associated with substantially lower RFU/OD 600 ratios. Thus, the KMI001-AHK4 detection culture must be incubated with test compounds at a constant temperature to obtain reproducible responses. In both (A,B) , data shown are means ± SD ( n ≥ 3).
    Thermomixer C Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermomixer c instrument/product/Eppendorf AG
    Average 99 stars, based on 452 article reviews
    Price from $9.99 to $1999.99
    thermomixer c instrument - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of temperature on AHK4 signaling. Signal to bacterial density (RFU to OD 600 ) ratios, and bacterial densities, obtained following incubation (for indicated times) of E. coli KMI001-AHK4 at 25 and 29°C in M9-505 medium supplemented with 1 μM tZ. 200 μl portions of KMI001-AHK4 detection culture were dispensed into two 96-well plates with tZ. One was cultivated at 25°C, and the other at 29°C, for 5 h with shaking at 800 rpm in a ThermoMixer C (Eppendorf). Every 30 min. three 30 μl samples were taken from both sets of E. coli suspensions, their optical density was measured then 0.3 μl of 50 mM 4-MUGal was added and incubation continued in a 384-well plate at 37°C with shaking at 1,400 rpm for 30 min. Then the reaction was stopped by adding 9 μl of 1.2 M sodium carbonate and fluorescence was measured. (A) The RFU/OD 600 ratios clearly show that detection culture cultivated at 29°C was much less sensitive than otherwise identically cultivated culture at 25°C. (B) Growth curves of detection cultures follow the classical trend, but in contrast to expectations the higher densities of E. coli cultivated at 29°C are associated with substantially lower RFU/OD 600 ratios. Thus, the KMI001-AHK4 detection culture must be incubated with test compounds at a constant temperature to obtain reproducible responses. In both (A,B) , data shown are means ± SD ( n ≥ 3).

    Journal: Frontiers in Plant Science

    Article Title: High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4

    doi: 10.3389/fpls.2017.00947

    Figure Lengend Snippet: Effect of temperature on AHK4 signaling. Signal to bacterial density (RFU to OD 600 ) ratios, and bacterial densities, obtained following incubation (for indicated times) of E. coli KMI001-AHK4 at 25 and 29°C in M9-505 medium supplemented with 1 μM tZ. 200 μl portions of KMI001-AHK4 detection culture were dispensed into two 96-well plates with tZ. One was cultivated at 25°C, and the other at 29°C, for 5 h with shaking at 800 rpm in a ThermoMixer C (Eppendorf). Every 30 min. three 30 μl samples were taken from both sets of E. coli suspensions, their optical density was measured then 0.3 μl of 50 mM 4-MUGal was added and incubation continued in a 384-well plate at 37°C with shaking at 1,400 rpm for 30 min. Then the reaction was stopped by adding 9 μl of 1.2 M sodium carbonate and fluorescence was measured. (A) The RFU/OD 600 ratios clearly show that detection culture cultivated at 29°C was much less sensitive than otherwise identically cultivated culture at 25°C. (B) Growth curves of detection cultures follow the classical trend, but in contrast to expectations the higher densities of E. coli cultivated at 29°C are associated with substantially lower RFU/OD 600 ratios. Thus, the KMI001-AHK4 detection culture must be incubated with test compounds at a constant temperature to obtain reproducible responses. In both (A,B) , data shown are means ± SD ( n ≥ 3).

    Article Snippet: 0.5 μl of 50 mM of the β-galactosidase substrate (4-MUGal in DMSO, final concentration 0.80 mM) was added to all wells and the plate was incubated at 37°C with shaking at 1,400 rpm for 30 min in a ThermoMixer C instrument (Eppendorf).

    Techniques: Incubation, Fluorescence

    ( A , B ) Adsorption isotherms of DNA on sepiolite in the presence of various polyvalent cations. Each point has error bars for 3 different experiments. Reaction conditions: 10 mM Tris-HCl pH = 7.5 and a sepiolite concentration of 1 mg/ml. ( C ) The effect of the presence of cations with different valences on DNA adsorption. Reaction conditions: 10 mM Tris-HCl pH = 7.5, salmon sperm DNA and sepiolite concentrations of 615 ng·μl −1 and 1 mg/ml, respectively. ( D , E ) Adsorption isotherms of different DNA conformations on sepiolite. Reaction conditions: 10 mM Tris-HCl pH = 7.5, 5 mM MgCl 2 , sepiolite concentration of 1 mg/ml; 50 μg of sepiolite was used in each experiment. Adsorption occurred at 25 °C for 24 hours under agitation at 700 rpm using an Eppendorf Thermomixer.

    Journal: Scientific Reports

    Article Title: Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells

    doi: 10.1038/srep36341

    Figure Lengend Snippet: ( A , B ) Adsorption isotherms of DNA on sepiolite in the presence of various polyvalent cations. Each point has error bars for 3 different experiments. Reaction conditions: 10 mM Tris-HCl pH = 7.5 and a sepiolite concentration of 1 mg/ml. ( C ) The effect of the presence of cations with different valences on DNA adsorption. Reaction conditions: 10 mM Tris-HCl pH = 7.5, salmon sperm DNA and sepiolite concentrations of 615 ng·μl −1 and 1 mg/ml, respectively. ( D , E ) Adsorption isotherms of different DNA conformations on sepiolite. Reaction conditions: 10 mM Tris-HCl pH = 7.5, 5 mM MgCl 2 , sepiolite concentration of 1 mg/ml; 50 μg of sepiolite was used in each experiment. Adsorption occurred at 25 °C for 24 hours under agitation at 700 rpm using an Eppendorf Thermomixer.

    Article Snippet: All of the Sep/DNA mixtures were stirred for 24 h at 25 °C and 700 rpm using an Eppendorf Thermomixer.

    Techniques: Adsorption, Concentration Assay

    Response of the 17% graphite + 1% Ag@NDs electrodes after being mixed with glucose oxidase in the thermomixer for 30 min and 1 h for glucose concentration between 0–60 mM ( A ). Linear concentration range for t = 30 min and t = 1 h ( B ). The experimental error was calculated as the standard deviation for n = 3 measurements.

    Journal: Nanomaterials

    Article Title: Customized In Situ Functionalization of Nanodiamonds with Nanoparticles for Composite Carbon-Paste Electrodes

    doi: 10.3390/nano10061179

    Figure Lengend Snippet: Response of the 17% graphite + 1% Ag@NDs electrodes after being mixed with glucose oxidase in the thermomixer for 30 min and 1 h for glucose concentration between 0–60 mM ( A ). Linear concentration range for t = 30 min and t = 1 h ( B ). The experimental error was calculated as the standard deviation for n = 3 measurements.

    Article Snippet: The electrode was incubated with the GOD solution using a thermomixer (Thermomixer comfort, Eppendorf AG, Hamburg, Germany) at 600 rpm and 20 °C.

    Techniques: Concentration Assay, Standard Deviation