thermocycler  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher thermocycler
    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same <t>thermocycler</t> and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Thermo Fisher
    Average 99 stars, based on 235 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-02
    99/100 stars

    Images

    1) Product Images from "An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest"

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-18216-x

    Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same thermocycler and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.
    Figure Legend Snippet: Nested/PCR-RFLP of ( A ) Plasmodium falciparum samples, two patients from Pará State (Pf – PA) and Acre State (Pf – AC) and Pf 3D7 culture (diluted 1:100); ( B ) Plasmodium brasilianum/Plasmodium malariae , two NHPs (Pbr 2434 and Pbr 2620), two patients (Pm P3 and Pm l11) and Plasmodium brasilianum from MR4 (diluted 1:100 in water); ( C ) Negative controls of PCR: two uninfected humans (UH - 1 and 2), one uninfected NHP (UNHP), and negative control of PCR (NC - without DNA). Reactions were performed simultaneously in the same thermocycler and splited in different agarose gels. 3% Agarose gelstained with ethidium bromide. MM: 1 kb Plus Ladder. D: Digested; ND: Non Digested.

    Techniques Used: Nested PCR, Polymerase Chain Reaction, Negative Control

    Differential diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 9 infected human samples from Atlantic Forest in Rio de Janeiro/RJ (H2 – H9) and one from Amazon endemic region (H1) according to Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer, Calrsbad, CA, USA). Reactions were performed simultaneously in the same thermocycler and splited in different gels.D: Digested; ND: Non Digested. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).
    Figure Legend Snippet: Differential diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 9 infected human samples from Atlantic Forest in Rio de Janeiro/RJ (H2 – H9) and one from Amazon endemic region (H1) according to Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer, Calrsbad, CA, USA). Reactions were performed simultaneously in the same thermocycler and splited in different gels.D: Digested; ND: Non Digested. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Techniques Used: Infection, Nested PCR, Agarose Gel Electrophoresis, Staining, Positive Control, Negative Control

    Nested/PCR-RFLP of P. vivax DNA samples. 15 DNA samples from P. vivax infected individuals from different parts of Amazon: Porto Velho/Rondônia State, Brazil (PvPV/RO1 and 2), Guyana (PvGuy), Ariquemes/Rondônia State, Brazil (PvAri/RO), Venezuela (PvVen), French Guiana (PvFrGui), Novo progresso/Pará State, Brazil (PvNP/PA), Rio Pardo/Amazonas State, Brazil (PvRP/AM1, 2 and 3), Humaita/Amazonas State, Brazil (PvHu/Am1, 2, 3 and 4) and unknown city in Amazonas State, Brazil (PvAM) were used for Nested PCR amplification followed by digestion with Hpy CH4III restriction enzyme. 3% Agarose gel stained with ethidium bromide. Name tags above gels indicated the patients according to Additional file 2. Reactions were performed simultaneously in the same thermocycler and splited in different gels. MM: 1 kb Plus Ladder. D: Digested (8 μL of digestion); ND: Non Digested (the equivalent amount of PCR product used in the digestion, 6.5 μL of samples and 5 μL of controls); PC Pv: positive control of P. vivax (pool of samples from infected patient from Amazonia); PC Ps: positive control of P. simium ( Alouatta g. clamitans infected with P. simium ); NC: negative Control.
    Figure Legend Snippet: Nested/PCR-RFLP of P. vivax DNA samples. 15 DNA samples from P. vivax infected individuals from different parts of Amazon: Porto Velho/Rondônia State, Brazil (PvPV/RO1 and 2), Guyana (PvGuy), Ariquemes/Rondônia State, Brazil (PvAri/RO), Venezuela (PvVen), French Guiana (PvFrGui), Novo progresso/Pará State, Brazil (PvNP/PA), Rio Pardo/Amazonas State, Brazil (PvRP/AM1, 2 and 3), Humaita/Amazonas State, Brazil (PvHu/Am1, 2, 3 and 4) and unknown city in Amazonas State, Brazil (PvAM) were used for Nested PCR amplification followed by digestion with Hpy CH4III restriction enzyme. 3% Agarose gel stained with ethidium bromide. Name tags above gels indicated the patients according to Additional file 2. Reactions were performed simultaneously in the same thermocycler and splited in different gels. MM: 1 kb Plus Ladder. D: Digested (8 μL of digestion); ND: Non Digested (the equivalent amount of PCR product used in the digestion, 6.5 μL of samples and 5 μL of controls); PC Pv: positive control of P. vivax (pool of samples from infected patient from Amazonia); PC Ps: positive control of P. simium ( Alouatta g. clamitans infected with P. simium ); NC: negative Control.

    Techniques Used: Nested PCR, Infection, Amplification, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction, Positive Control, Negative Control

    Differential Diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 16 non-human primate samples: ( A ) 2 captive NHP from Rio de Janeiro/RJ ( Sapajus xanthosternos 2098; Cacajao melanocephalus 2302), one free-living Alouatta g. clamitans from Rio de Janeiro State (3636) and 6 free-living Alouatta g. clamitans from Joinville/SC, Brazil (J9, J11, J15, J20, J22, J25); ( B ) 7 Alouatta g. clamitans from CEPESBI, Indaial, SC, Brazil (Bl3, Bl6, Bl10, Bl28, Bl61, Bl64, Bl69), *Captive NHPs, all the other were free-living. More details about each sample see Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer). Reactions were performed simultaneously in the same thermocycler and splited in different gels. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).
    Figure Legend Snippet: Differential Diagnosis of Plasmodium simium infection by nested/PCR followed by a digestion with Hpy CH4III restriction enzyme of 16 non-human primate samples: ( A ) 2 captive NHP from Rio de Janeiro/RJ ( Sapajus xanthosternos 2098; Cacajao melanocephalus 2302), one free-living Alouatta g. clamitans from Rio de Janeiro State (3636) and 6 free-living Alouatta g. clamitans from Joinville/SC, Brazil (J9, J11, J15, J20, J22, J25); ( B ) 7 Alouatta g. clamitans from CEPESBI, Indaial, SC, Brazil (Bl3, Bl6, Bl10, Bl28, Bl61, Bl64, Bl69), *Captive NHPs, all the other were free-living. More details about each sample see Additional file 1. 3% agarose gel stained with ethidium bromide. MM:1 kb Plus Ladder (ThermoFischer). Reactions were performed simultaneously in the same thermocycler and splited in different gels. PC Pv: Positive Control for P. vivax , PC Ps: positive control for P. simium . NC: Negative Control (without DNA).

    Techniques Used: Infection, Nested PCR, Agarose Gel Electrophoresis, Staining, Positive Control, Negative Control

    Related Articles

    Amplification:

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequence analysis of rrs gene. ... Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems).

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest
    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min. .. The amplified fragments were visualized by electrophoresis on 2% agarose gels in 1x TAE buffer (40 mM Tris-acetate, 1 mM EDTA) with 5 μg/mL ethidium bromide (Invitrogen) in a horizontal system (Bio-Rad) at 100 V for 30 min. Gels were examined with a UV transilluminator (UVP - Bio-Doc System it).

    Article Title: Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis
    Article Snippet: .. After incubation at 50 °C for 30 min. and PCR activation (95 °C, 15 min.), the amplification comprised 35 cycles of denaturing (94 °C, 30 seconds), annealing (30 seconds), extension (72 °C, 60 seconds), and a final extension (72 °C, 10 min.) in a thermocycler (Applied Biosystems, California, USA). .. Products were resolved on agarose gels (2%) containing ethidium bromide (0.5 μg/ml) and analyzed (G-BOX gel doc, Syngene, Cambridge, UK) using Gene Tools (Version 4.01.04) software, values being normalized to their respective β-actin.

    Article Title: The α1AT and TIMP-1 Gene Polymorphism in the Development of Asthma
    Article Snippet: The primers as described previously were synthesized and used for amplification of α 1 AT and TIMP-1 genes [ ]. .. The PCR was carried out at optimized conditions [ ] using a thermocycler (Gene Amp 9700, Applied Biosystems, Life Technologies, Singapore).

    Article Title: Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase
    Article Snippet: .. The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C. .. The purified PCR products were ligated into the pET SUMO vector (Invitrogen, Life technologies).

    Article Title: Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)
    Article Snippet: Two Grande 1F (5′-AAA GAC CTC ACG AAA GGC CCA AGG-3′), Grande1R (5′-AAA TGG TTC ATG CCG ATT GCA CG-3′) primers (GenBank accession number X97604 ; ) were used for the PCR reaction, which in successive cycles enabled the amplification of the 500 bp retrotransposon region of Grande-1 and detection of the presence of maize chromatin in oat plants. .. The reaction mixture was placed in a thermocycler (2720 Thermal Cycler; Applied Biosystems, Foster City, CA, USA) and used the following thermal program: 1.

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene. .. The reaction was performed under the following sequential conditions: incubation at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s and 60 °C for 1 min. After 40 cycles, a dissociation curve for each amplicon was created.

    Article Title: Multiplexed Illumina sequencing libraries from picogram quantities of DNA
    Article Snippet: Paragraph title: Library amplification by qPCR ... The thermocycler used in these experiments was an Applied Biosystems 7500 Fast Real-Time PCR System.

    Expressing:

    Article Title: Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase
    Article Snippet: Paragraph title: Construction of expression vectors of cDNAs coding for wild type (WT), N-truncated (NT) and N- and C-truncated (NCT) type trehalases ... The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C.

    Synthesized:

    Article Title: The α1AT and TIMP-1 Gene Polymorphism in the Development of Asthma
    Article Snippet: The primers as described previously were synthesized and used for amplification of α 1 AT and TIMP-1 genes [ ]. .. The PCR was carried out at optimized conditions [ ] using a thermocycler (Gene Amp 9700, Applied Biosystems, Life Technologies, Singapore).

    Quantitative RT-PCR:

    Article Title: Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil
    Article Snippet: Extraction plates were heated on a thermocycler (Applied Biosystems, Foster City, CA, USA) at 56 °C for 5 min, followed by 98 °C for 15 min, and then were stored at 12 °C until PCR. .. Detection of viral RNA was performed via Real-time PCR (RT-qPCR) using the Gotaq® Probe 1-step RT-qPCR System (Promega, Madison, WI, USA) according to the manufacturer’s protocol.

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: OligoPerfect™ Designer (Invitrogen™) was used to design quantitative real-time (RT-) PCR primers. .. Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene.

    Article Title: High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays
    Article Snippet: Paragraph title: QRT-PCR for miRNA ... Each RNA sample (5 ng) was reverse transcribed using a TaqMan MicroRNA assay (Ambion) in a 15-µl reaction performed on a thermocycler (Applied Biosystems 9700) for 30 min at 16°C, 30 min at 42°C, 5 min at 85°C, and then held at 4°C.

    Article Title: A 20-Gene Set Predictive of Progression to Severe Dengue
    Article Snippet: Paragraph title: High-throughput microfluidic qRT-PCR assays ... Preamplification was performed on a thermocycler following addition of the TaqMan PreAmp Master Mix Kit (Invitrogen) to the pooled Taqman assays and cDNA.

    SYBR Green Assay:

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene. .. Each reaction was performed in 10 μL (total volume) containing 2.5 μL of SYBR Green ROX Plus PCR Mix (LGC Biotecnologia), 2 μL of cDNA (diluted 1:40), 4.7 μL of distilled water and 0.2 μM of each primer (forward and reverse).

    Article Title: Multiplexed Illumina sequencing libraries from picogram quantities of DNA
    Article Snippet: Reactions were stopped at the end of the extension, after SYBR green reported reaction kinetics in the log phase for several cycles. .. The thermocycler used in these experiments was an Applied Biosystems 7500 Fast Real-Time PCR System.

    Incubation:

    Article Title: Identification of Biomarkers for Esophageal Squamous Cell Carcinoma Using Feature Selection and Decision Tree Methods
    Article Snippet: .. For the appropriate efficiency of reverse transcription, 1 μ g total RNA was placed in a microcentrifuge tube and incubated at 70°C for 10 minutes in a thermocycler (Gene Amp PCR system 9700, Applied Biosystems). ..

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: Ten microliters of each amplicon was purified in order to remove residual deoxynucleotides and primers by adding 2.0 μl of 1-U/μl rAPid alkaline phosphatase (Roche Diagnostics, Rotkreuz, Switzerland), 0.4 μl of the corresponding buffer, and 0.1 μl of exonuclease I (New England BioLabs, Ipswich, MA, USA) and by incubation at 37°C for 20 min and then at 80°C for 20 min to inactivate the enzymes. .. Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems).

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest
    Article Snippet: .. The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min. .. For the second PCR 1–3 μL of the primary product was used as template.

    Article Title: Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis
    Article Snippet: .. After incubation at 50 °C for 30 min. and PCR activation (95 °C, 15 min.), the amplification comprised 35 cycles of denaturing (94 °C, 30 seconds), annealing (30 seconds), extension (72 °C, 60 seconds), and a final extension (72 °C, 10 min.) in a thermocycler (Applied Biosystems, California, USA). .. Products were resolved on agarose gels (2%) containing ethidium bromide (0.5 μg/ml) and analyzed (G-BOX gel doc, Syngene, Cambridge, UK) using Gene Tools (Version 4.01.04) software, values being normalized to their respective β-actin.

    Article Title: Introduction of apple ANR genes into tobacco inhibits expression of both CHI and DFR genes in flowers, leading to loss of anthocyanin
    Article Snippet: .. Flavonoid analysis Anthocyanins and flavonols were extracted from 50 mg of finely-ground tissues in 1 ml of 1% HCl/methanol (v/v) at room temperature in the dark with continuous shaking for 1 h, and centrifuged at 13, 000 rpm for 15 min. A total of 100 μl of supernatant was transferred to a fresh tube, acid-hydrolysed by adding 30 μl of 3 N HCl, and incubated at 70 °C for 1 h in a Thermo Hybaid MBS 0.25s thermocycler (Thermo Scientific). ..

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene. .. The reaction was performed under the following sequential conditions: incubation at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s and 60 °C for 1 min. After 40 cycles, a dissociation curve for each amplicon was created.

    Mass Spectrometry:

    Article Title: Introduction of apple ANR genes into tobacco inhibits expression of both CHI and DFR genes in flowers, leading to loss of anthocyanin
    Article Snippet: Flavonoid analysis Anthocyanins and flavonols were extracted from 50 mg of finely-ground tissues in 1 ml of 1% HCl/methanol (v/v) at room temperature in the dark with continuous shaking for 1 h, and centrifuged at 13, 000 rpm for 15 min. A total of 100 μl of supernatant was transferred to a fresh tube, acid-hydrolysed by adding 30 μl of 3 N HCl, and incubated at 70 °C for 1 h in a Thermo Hybaid MBS 0.25s thermocycler (Thermo Scientific). .. Flavonoids were identified using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and their contents were calculated by comparison with commercial standards, including kaempferol, quercetin, cyanidin, catechin, and epicatechin (Sigma).

    Real-time Polymerase Chain Reaction:

    Article Title: Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil
    Article Snippet: Extraction plates were heated on a thermocycler (Applied Biosystems, Foster City, CA, USA) at 56 °C for 5 min, followed by 98 °C for 15 min, and then were stored at 12 °C until PCR. .. Detection of viral RNA was performed via Real-time PCR (RT-qPCR) using the Gotaq® Probe 1-step RT-qPCR System (Promega, Madison, WI, USA) according to the manufacturer’s protocol.

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: Paragraph title: RNA isolation and qPCR ... Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene.

    Article Title: High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays
    Article Snippet: Each RNA sample (5 ng) was reverse transcribed using a TaqMan MicroRNA assay (Ambion) in a 15-µl reaction performed on a thermocycler (Applied Biosystems 9700) for 30 min at 16°C, 30 min at 42°C, 5 min at 85°C, and then held at 4°C. .. Real-time PCR following reverse transcription (RT) was performed on an Applied Biosystems 7900HT using the same kit.

    Article Title: A 20-Gene Set Predictive of Progression to Severe Dengue
    Article Snippet: High-throughput microfluidic qRT-PCR assays The Biomark Microfluidic qPCR Array was used to quantify the individual transcripts of the signature at the Stanford Human Immune Monitoring Center, as previously described ( ). .. Preamplification was performed on a thermocycler following addition of the TaqMan PreAmp Master Mix Kit (Invitrogen) to the pooled Taqman assays and cDNA.

    Article Title: Multiplexed Illumina sequencing libraries from picogram quantities of DNA
    Article Snippet: .. The thermocycler used in these experiments was an Applied Biosystems 7500 Fast Real-Time PCR System. .. Illumina sequencing and data analysis Libraries were diluted and pooled for cluster generation and sequence analysis on one lane of an Illumina HiSeq2000 by a local NGS service provider, who sequenced the library using standard manufacturer’s procedures.

    Article Title: The anti-CMS technique for genome-wide mapping of 5-hydroxymethylcytosine
    Article Snippet: .. Adaptive Focused Acoustics (AFA) S2 (Covaris) DynaMag-2 (Invitrogen, cat. no. 123-21D) Gel running apparatus (Thermo Scientific) NanoDrop 1000 (Thermo Scientific) Thermocycler (Applied Biosystems, cat. no. 2720) Nutator (Southwest Science, cat. no. SB3D2300) Thermomixer (Eppendorf) Desktop centrifuge (Eppendorf, cat. no. 5424) Qubit 2.0 fluorometer (Invitrogen, cat. no. ) StepOnePlus real-time PCR system (Life Technologies) Microcentrifuge (VWR, cat. no. C1413-VWR230) Bioanalyzer (Agilent) P20 pipette tips ..

    Transfection:

    Article Title: Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase
    Article Snippet: The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C. .. The transfected cells were plated in LB agar containing kanamycin (50 µg/mL) and were grown overnight.

    Chromatography:

    Article Title: Introduction of apple ANR genes into tobacco inhibits expression of both CHI and DFR genes in flowers, leading to loss of anthocyanin
    Article Snippet: Flavonoid analysis Anthocyanins and flavonols were extracted from 50 mg of finely-ground tissues in 1 ml of 1% HCl/methanol (v/v) at room temperature in the dark with continuous shaking for 1 h, and centrifuged at 13, 000 rpm for 15 min. A total of 100 μl of supernatant was transferred to a fresh tube, acid-hydrolysed by adding 30 μl of 3 N HCl, and incubated at 70 °C for 1 h in a Thermo Hybaid MBS 0.25s thermocycler (Thermo Scientific). .. Flavonoids were identified using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and their contents were calculated by comparison with commercial standards, including kaempferol, quercetin, cyanidin, catechin, and epicatechin (Sigma).

    Concentration Assay:

    Article Title: Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)
    Article Snippet: DNA concentration was measured at 260 nm with a spectrophotometer NanoDrop 2000c (Thermo Scientific, USA). .. The reaction mixture was placed in a thermocycler (2720 Thermal Cycler; Applied Biosystems, Foster City, CA, USA) and used the following thermal program: 1.

    Transferring:

    Article Title: The anti-CMS technique for genome-wide mapping of 5-hydroxymethylcytosine
    Article Snippet: .. Adaptive Focused Acoustics (AFA) S2 (Covaris) DynaMag-2 (Invitrogen, cat. no. 123-21D) Gel running apparatus (Thermo Scientific) NanoDrop 1000 (Thermo Scientific) Thermocycler (Applied Biosystems, cat. no. 2720) Nutator (Southwest Science, cat. no. SB3D2300) Thermomixer (Eppendorf) Desktop centrifuge (Eppendorf, cat. no. 5424) Qubit 2.0 fluorometer (Invitrogen, cat. no. ) StepOnePlus real-time PCR system (Life Technologies) Microcentrifuge (VWR, cat. no. C1413-VWR230) Bioanalyzer (Agilent) P20 pipette tips ..

    TaqMan microRNA Assay:

    Article Title: High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays
    Article Snippet: .. Each RNA sample (5 ng) was reverse transcribed using a TaqMan MicroRNA assay (Ambion) in a 15-µl reaction performed on a thermocycler (Applied Biosystems 9700) for 30 min at 16°C, 30 min at 42°C, 5 min at 85°C, and then held at 4°C. .. Real-time PCR following reverse transcription (RT) was performed on an Applied Biosystems 7900HT using the same kit.

    Sequencing:

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: .. Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems). .. The sequences were edited using the Sequencher software version 5.0 (Gene Code Corp., Ann Arbor, MI, USA).

    Article Title: The α1AT and TIMP-1 Gene Polymorphism in the Development of Asthma
    Article Snippet: The PCR was carried out at optimized conditions [ ] using a thermocycler (Gene Amp 9700, Applied Biosystems, Life Technologies, Singapore). .. The amplified PCR products were purified and subjected to sequencing using a 3730 sequencer (ABI Prism, USA).

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: The DNA sequence of AgraVg (accession number: M72980.1 ) was retrieved from GenBank ( www.ncbi.nlm.nih.gov/genbank ). .. Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene.

    Recombinant:

    Article Title: Identification of Biomarkers for Esophageal Squamous Cell Carcinoma Using Feature Selection and Decision Tree Methods
    Article Snippet: For the appropriate efficiency of reverse transcription, 1 μ g total RNA was placed in a microcentrifuge tube and incubated at 70°C for 10 minutes in a thermocycler (Gene Amp PCR system 9700, Applied Biosystems). .. The final concentrations of reaction component were 5 mM MgCl2 ; 1X reverse transcription buffer (10 mM tris-HCl (pH 9.0 at 25°C); 50 mM KCl; 0.1% triton X-100); 1 mM each dNTP; 1 u/μ L recombinant RNasin ribonuclease inhibitor; 15 u/μ g AMV reverse transcriptase (high conc.); and 0.5 μ g oligo (dT)15 .

    Genotyping Assay:

    Article Title: Maternal and fetal predictors of fetal viral load and death in third trimester, type 2 porcine reproductive and respiratory syndrome virus infected pregnant gilts
    Article Snippet: Genomic DNA was diluted to 10 ng/μL and 2 μL of DNA added to the DNA assay mix containing 5 μL of DNA TaqMan Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems 4324018), 0.5 μL of TaqMan-MGB genotyping assay mix (20X), and 2.5 μL of water. .. After sealing the plate it was vortexed and centrifuged before PCR with thermocycler conditions: 60 °C for 30 s, 95 °C for 10 min, 95 °C for 15 s, 60 °C for 1 min, repeating steps 3–4 for 40 cycles and 60 °C for 30 s. The assay was run on a StepOne Plus (Life Technologies).

    DNA Extraction:

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequence analysis of rrs gene. ... Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems).

    Article Title: Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)
    Article Snippet: Paragraph title: DNA extraction and PCR analyses ... The reaction mixture was placed in a thermocycler (2720 Thermal Cycler; Applied Biosystems, Foster City, CA, USA) and used the following thermal program: 1.

    Isolation:

    Article Title: Identification of Biomarkers for Esophageal Squamous Cell Carcinoma Using Feature Selection and Decision Tree Methods
    Article Snippet: Reverse Transcription After RNA isolation, cDNA was prepared from each sample by Reverse Transcription System (Cat: A3500, Promega Corporation, USA). .. For the appropriate efficiency of reverse transcription, 1 μ g total RNA was placed in a microcentrifuge tube and incubated at 70°C for 10 minutes in a thermocycler (Gene Amp PCR system 9700, Applied Biosystems).

    Article Title: Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis
    Article Snippet: Paragraph title: Isolation of total RNA and Reverse transcriptase-PCR analysis ... After incubation at 50 °C for 30 min. and PCR activation (95 °C, 15 min.), the amplification comprised 35 cycles of denaturing (94 °C, 30 seconds), annealing (30 seconds), extension (72 °C, 60 seconds), and a final extension (72 °C, 10 min.) in a thermocycler (Applied Biosystems, California, USA).

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: Paragraph title: RNA isolation and qPCR ... Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene.

    Article Title: High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays
    Article Snippet: QRT-PCR for miRNA Total RNA was isolated from 2×106 cells using Trizol reagent (Invitrogen) according to the manufacturer's instruction. .. Each RNA sample (5 ng) was reverse transcribed using a TaqMan MicroRNA assay (Ambion) in a 15-µl reaction performed on a thermocycler (Applied Biosystems 9700) for 30 min at 16°C, 30 min at 42°C, 5 min at 85°C, and then held at 4°C.

    Size-exclusion Chromatography:

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest
    Article Snippet: .. The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min. .. For the second PCR 1–3 μL of the primary product was used as template.

    Labeling:

    Article Title: Maternal and fetal predictors of fetal viral load and death in third trimester, type 2 porcine reproductive and respiratory syndrome virus infected pregnant gilts
    Article Snippet: The genotype of the WUR10000125 SNP was determined by a TaqMan assay (Forward primer: 5′ AGA CCT AGA ATC TCC ACA GAA TTT CCA 3′; Reverse primer: 5′ AAG TTA GAA TCT GCG CGA ATC GA 3′; Taqman MGB probe (VIC labeled): 5′ CTG GGT GAT A AA TAA AT 3′; Taqman MGB probe (FAM labeled): 5′ TGG GTGA T G A ATA AAT 3′; where the underlined bases are the A/G polymorphism scored). .. After sealing the plate it was vortexed and centrifuged before PCR with thermocycler conditions: 60 °C for 30 s, 95 °C for 10 min, 95 °C for 15 s, 60 °C for 1 min, repeating steps 3–4 for 40 cycles and 60 °C for 30 s. The assay was run on a StepOne Plus (Life Technologies).

    Purification:

    Article Title: Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil
    Article Snippet: Extraction plates were heated on a thermocycler (Applied Biosystems, Foster City, CA, USA) at 56 °C for 5 min, followed by 98 °C for 15 min, and then were stored at 12 °C until PCR. .. In this type of extraction, there is no need for sample purification; in the final solution, there is RNA and DNA from the extracted material [ ].

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: .. Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems). .. The sequences were edited using the Sequencher software version 5.0 (Gene Code Corp., Ann Arbor, MI, USA).

    Article Title: The α1AT and TIMP-1 Gene Polymorphism in the Development of Asthma
    Article Snippet: The PCR was carried out at optimized conditions [ ] using a thermocycler (Gene Amp 9700, Applied Biosystems, Life Technologies, Singapore). .. The amplified PCR products were purified and subjected to sequencing using a 3730 sequencer (ABI Prism, USA).

    Article Title: Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase
    Article Snippet: The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C. .. The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C.

    Polymerase Chain Reaction:

    Article Title: Identification of Biomarkers for Esophageal Squamous Cell Carcinoma Using Feature Selection and Decision Tree Methods
    Article Snippet: .. For the appropriate efficiency of reverse transcription, 1 μ g total RNA was placed in a microcentrifuge tube and incubated at 70°C for 10 minutes in a thermocycler (Gene Amp PCR system 9700, Applied Biosystems). ..

    Article Title: Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil
    Article Snippet: .. Extraction plates were heated on a thermocycler (Applied Biosystems, Foster City, CA, USA) at 56 °C for 5 min, followed by 98 °C for 15 min, and then were stored at 12 °C until PCR. .. In this type of extraction, there is no need for sample purification; in the final solution, there is RNA and DNA from the extracted material [ ].

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: .. Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems). .. The sequences were edited using the Sequencher software version 5.0 (Gene Code Corp., Ann Arbor, MI, USA).

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest
    Article Snippet: .. The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min. .. For the second PCR 1–3 μL of the primary product was used as template.

    Article Title: Maternal and fetal predictors of fetal viral load and death in third trimester, type 2 porcine reproductive and respiratory syndrome virus infected pregnant gilts
    Article Snippet: .. After sealing the plate it was vortexed and centrifuged before PCR with thermocycler conditions: 60 °C for 30 s, 95 °C for 10 min, 95 °C for 15 s, 60 °C for 1 min, repeating steps 3–4 for 40 cycles and 60 °C for 30 s. The assay was run on a StepOne Plus (Life Technologies). ..

    Article Title: Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis
    Article Snippet: .. After incubation at 50 °C for 30 min. and PCR activation (95 °C, 15 min.), the amplification comprised 35 cycles of denaturing (94 °C, 30 seconds), annealing (30 seconds), extension (72 °C, 60 seconds), and a final extension (72 °C, 10 min.) in a thermocycler (Applied Biosystems, California, USA). .. Products were resolved on agarose gels (2%) containing ethidium bromide (0.5 μg/ml) and analyzed (G-BOX gel doc, Syngene, Cambridge, UK) using Gene Tools (Version 4.01.04) software, values being normalized to their respective β-actin.

    Article Title: The α1AT and TIMP-1 Gene Polymorphism in the Development of Asthma
    Article Snippet: .. The PCR was carried out at optimized conditions [ ] using a thermocycler (Gene Amp 9700, Applied Biosystems, Life Technologies, Singapore). .. The amplified PCR products were purified and subjected to sequencing using a 3730 sequencer (ABI Prism, USA).

    Article Title: Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase
    Article Snippet: .. The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C. .. The purified PCR products were ligated into the pET SUMO vector (Invitrogen, Life technologies).

    Article Title: Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)
    Article Snippet: Paragraph title: DNA extraction and PCR analyses ... The reaction mixture was placed in a thermocycler (2720 Thermal Cycler; Applied Biosystems, Foster City, CA, USA) and used the following thermal program: 1.

    Article Title: Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females
    Article Snippet: The primers were validated with a performance dissociation protocol to detect the presence of primer dimers and the production of a single PCR product. .. Reverse transcription-qPCR assays were set up by using a 7500 Fast (Applied Biosystems) thermocycler and specific primers for each gene.

    Article Title: High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays
    Article Snippet: Each RNA sample (5 ng) was reverse transcribed using a TaqMan MicroRNA assay (Ambion) in a 15-µl reaction performed on a thermocycler (Applied Biosystems 9700) for 30 min at 16°C, 30 min at 42°C, 5 min at 85°C, and then held at 4°C. .. Specifically, 1.33 µl of RT product was used in a 20-µl PCR system.

    Article Title: Multiplexed Illumina sequencing libraries from picogram quantities of DNA
    Article Snippet: Library amplification by qPCR PCR reactions (50 uL) consisted of 1X Phusion HF master mix (NEB), 0.2 uM universal primer, 0.2 uM barcoded primer, 1X SYBR Green I (Invitrogen), and 0.5 uL Rox (USB). .. The thermocycler used in these experiments was an Applied Biosystems 7500 Fast Real-Time PCR System.

    TaqMan Assay:

    Article Title: Maternal and fetal predictors of fetal viral load and death in third trimester, type 2 porcine reproductive and respiratory syndrome virus infected pregnant gilts
    Article Snippet: The genotype of the WUR10000125 SNP was determined by a TaqMan assay (Forward primer: 5′ AGA CCT AGA ATC TCC ACA GAA TTT CCA 3′; Reverse primer: 5′ AAG TTA GAA TCT GCG CGA ATC GA 3′; Taqman MGB probe (VIC labeled): 5′ CTG GGT GAT A AA TAA AT 3′; Taqman MGB probe (FAM labeled): 5′ TGG GTGA T G A ATA AAT 3′; where the underlined bases are the A/G polymorphism scored). .. After sealing the plate it was vortexed and centrifuged before PCR with thermocycler conditions: 60 °C for 30 s, 95 °C for 10 min, 95 °C for 15 s, 60 °C for 1 min, repeating steps 3–4 for 40 cycles and 60 °C for 30 s. The assay was run on a StepOne Plus (Life Technologies).

    Nested PCR:

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest
    Article Snippet: Paragraph title: Nested PCR and RFLP ... The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min.

    RNA Extraction:

    Article Title: Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil
    Article Snippet: RNA extraction was performed with 50 μL of macerated mosquito using 50 μL of squash buffer solution [Tris 99% (Inlab, São Paulo Brazil), sodium chloride (Synth, Diadema, Brazil), EDTA (Synth, Diadema, Brazil)] and added Proteinase K (Qiagen, Hilden, Germany) 0.6 μL in Hard-Shell® 96-Well PCR Plates (low profile, thin walled, skirted, and white/clear) (BioRad, Hercules, CA, USA). .. Extraction plates were heated on a thermocycler (Applied Biosystems, Foster City, CA, USA) at 56 °C for 5 min, followed by 98 °C for 15 min, and then were stored at 12 °C until PCR.

    Plasmid Preparation:

    Article Title: Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase
    Article Snippet: 2.2 Construction of expression vectors of cDNAs coding for wild type (WT), N-truncated (NT) and N- and C-truncated (NCT) type trehalases The cDNAs coding for trehalase and truncated trehalases were amplified by polymerase chain-reaction (PCR) with the primers listed in , using the plasmid pGEMT containing the mature (without the signal peptide) coding sequence of trehalase (WT, which is SfTre1, GenBank accession no. DQ447188) as a template. .. The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C.

    Software:

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems). .. The sequences were edited using the Sequencher software version 5.0 (Gene Code Corp., Ann Arbor, MI, USA).

    Article Title: Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis
    Article Snippet: After incubation at 50 °C for 30 min. and PCR activation (95 °C, 15 min.), the amplification comprised 35 cycles of denaturing (94 °C, 30 seconds), annealing (30 seconds), extension (72 °C, 60 seconds), and a final extension (72 °C, 10 min.) in a thermocycler (Applied Biosystems, California, USA). .. Products were resolved on agarose gels (2%) containing ethidium bromide (0.5 μg/ml) and analyzed (G-BOX gel doc, Syngene, Cambridge, UK) using Gene Tools (Version 4.01.04) software, values being normalized to their respective β-actin.

    Electrophoresis:

    Article Title: An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest
    Article Snippet: The PCR assays were performed in a thermocycler (Veriti 96 wells, Applied Biosystems) with the following cycling parameters: an initial denaturation at 94 °C for 2 min followed by 40 cycles of denaturation at 94 °C for 30 sec, annealing at 54 °C for 20 sec and extension at 72 °C for 30 sec followed by a final extension incubation at 72 °C for 2 min. .. The amplified fragments were visualized by electrophoresis on 2% agarose gels in 1x TAE buffer (40 mM Tris-acetate, 1 mM EDTA) with 5 μg/mL ethidium bromide (Invitrogen) in a horizontal system (Bio-Rad) at 100 V for 30 min. Gels were examined with a UV transilluminator (UVP - Bio-Doc System it).

    Multiplex Assay:

    Article Title: Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017–2018 in Minas Gerais State, Brazil
    Article Snippet: Extraction plates were heated on a thermocycler (Applied Biosystems, Foster City, CA, USA) at 56 °C for 5 min, followed by 98 °C for 15 min, and then were stored at 12 °C until PCR. .. Multiplex reactions were performed with the primers and probes described in .

    Article Title: The α1AT and TIMP-1 Gene Polymorphism in the Development of Asthma
    Article Snippet: The PCR was carried out at optimized conditions [ ] using a thermocycler (Gene Amp 9700, Applied Biosystems, Life Technologies, Singapore). .. The SNPs were first identified by full length sequencing of PCR products of few subjects and subsequently large batches of samples were analysed for polymorphic genotypes by SNapshot Multiplex Kit (ABI Prism, USA) using SNapshot primers ( ).

    Positron Emission Tomography:

    Article Title: Conformational changes on ligand binding in wild-type and mutants from Spodoptera frugiperda midgut trehalase
    Article Snippet: The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C. .. The PCR was performed in a thermocycler (AB Applied Biosystems) with TAQ DNA polymerase (Invitrogen TM Life Technologies), according to manufacturer's instructions, with 30 cycles of amplification (94 °C for 30 s; 50 °C for 45 s; 72 °C for 120 s), followed by 10 min at 72 °C.

    Ethanol Precipitation:

    Article Title: Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, “ Porphyromonas katsikii”
    Article Snippet: .. Five picomoles of the appropriate primer was added to about 20 ng (1.0 μl) of the purified PCR product and sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems) in a thermocycler for 25 cycles of 96°C for 10 s, 50°C for 5 s, and 60°C for 1 min. After ethanol precipitation of the sequencing products, the samples were run on an ABI Prism 3130 XL genetic analyzer (Applied Biosystems). .. The sequences were edited using the Sequencher software version 5.0 (Gene Code Corp., Ann Arbor, MI, USA).

    Spectrophotometry:

    Article Title: Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)
    Article Snippet: DNA concentration was measured at 260 nm with a spectrophotometer NanoDrop 2000c (Thermo Scientific, USA). .. The reaction mixture was placed in a thermocycler (2720 Thermal Cycler; Applied Biosystems, Foster City, CA, USA) and used the following thermal program: 1.

    Activation Assay:

    Article Title: Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis
    Article Snippet: .. After incubation at 50 °C for 30 min. and PCR activation (95 °C, 15 min.), the amplification comprised 35 cycles of denaturing (94 °C, 30 seconds), annealing (30 seconds), extension (72 °C, 60 seconds), and a final extension (72 °C, 10 min.) in a thermocycler (Applied Biosystems, California, USA). .. Products were resolved on agarose gels (2%) containing ethidium bromide (0.5 μg/ml) and analyzed (G-BOX gel doc, Syngene, Cambridge, UK) using Gene Tools (Version 4.01.04) software, values being normalized to their respective β-actin.

    CTG Assay:

    Article Title: Maternal and fetal predictors of fetal viral load and death in third trimester, type 2 porcine reproductive and respiratory syndrome virus infected pregnant gilts
    Article Snippet: The genotype of the WUR10000125 SNP was determined by a TaqMan assay (Forward primer: 5′ AGA CCT AGA ATC TCC ACA GAA TTT CCA 3′; Reverse primer: 5′ AAG TTA GAA TCT GCG CGA ATC GA 3′; Taqman MGB probe (VIC labeled): 5′ CTG GGT GAT A AA TAA AT 3′; Taqman MGB probe (FAM labeled): 5′ TGG GTGA T G A ATA AAT 3′; where the underlined bases are the A/G polymorphism scored). .. After sealing the plate it was vortexed and centrifuged before PCR with thermocycler conditions: 60 °C for 30 s, 95 °C for 10 min, 95 °C for 15 s, 60 °C for 1 min, repeating steps 3–4 for 40 cycles and 60 °C for 30 s. The assay was run on a StepOne Plus (Life Technologies).

    High Throughput Screening Assay:

    Article Title: A 20-Gene Set Predictive of Progression to Severe Dengue
    Article Snippet: Paragraph title: High-throughput microfluidic qRT-PCR assays ... Preamplification was performed on a thermocycler following addition of the TaqMan PreAmp Master Mix Kit (Invitrogen) to the pooled Taqman assays and cDNA.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Thermo Fisher oligos thermocycler aari enzyme
    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed <t>oligos</t> corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between <t>AarI</t> and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.
    Oligos Thermocycler Aari Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligos thermocycler aari enzyme/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligos thermocycler aari enzyme - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    99
    Thermo Fisher thermocycler
    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed <t>oligos</t> corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between <t>AarI</t> and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.
    Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Thermo Fisher
    Average 99 stars, based on 235 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher pcr sprint thermocycler
    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed <t>oligos</t> corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between <t>AarI</t> and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.
    Pcr Sprint Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr sprint thermocycler/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pcr sprint thermocycler - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher simpliamp thermocycler
    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed <t>oligos</t> corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between <t>AarI</t> and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.
    Simpliamp Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/simpliamp thermocycler/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    simpliamp thermocycler - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed oligos corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between AarI and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.

    Journal: Current protocols in molecular biology

    Article Title: CASAAV: a CRISPR based platform for rapid dissection of gene function in vivo

    doi: 10.1002/cpmb.46

    Figure Lengend Snippet: Insertion of gRNA sequences into AAV vector gRNAs are inserted into AAV plasmid containing U6 promoters and cardiac troponin T (cTNT) promoter-driven Cre recombinase. Annealed oligos corresponding to the targeting portions of gRNA1 and gRNA2 are cloned between AarI and SapI sites, respectively. The entire construct is contained within AAV inverted terminal repeat sequences, allowing for packaging into AAV capsids. Examples of gRNAs designed against Gata4 are shown.

    Article Snippet: AAV-U6gRNA1-U6gRNA2-TnT-Cre Vector (Addgene #87682) gRNA sequences from Basic Protocol 1/DNA oligos Thermocycler AarI enzyme and buffer (ThermoFisher ER1581) SapI enzyme and buffer (NEB R0569L) 37°C incubator 37°C shaking incubator Agarose (GeneMate E-3120-500) 50× TAE (Boston BioProducts BM250) Ethidium bromide (Sigma E7637) DNA loading dye (NEB B7024S) Gel electrophoresis chamber UV transilluminator (365 nm) Gel purification kit (Invitrogen K210012) Quick ligation kit (NEB M2200L) Chemically competent cells such as ThermoFisher MAX Efficiency Stbl2 Competent Cells (Cat# 10268019).

    Techniques: Plasmid Preparation, Clone Assay, Construct