Structured Review

Roche thermocycler
Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thermocycler/product/Roche
Average 97 stars, based on 55 article reviews
Price from $9.99 to $1999.99
thermocycler - by Bioz Stars, 2020-02
97/100 stars

Images

Related Articles

Amplification:

Article Title: Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells
Article Snippet: The resulting cDNA was subjected to real-time polymerase chain reaction (PCR) analysis by rapid cycling in glass capillaries with a thermocycler (Light-Cycler; Roche Molecular Biochemicals, Milano, Italy). .. The amplification protocol consisted of an initial denaturation step at 95°C for 10 min.

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: To obtain a probe complementary to the 5′ part of the ITS1, the D-A2 rDNA fragment from S. cerevisiae was amplified by PCR and subcloned into pGEM4. .. The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche).

Article Title: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia
Article Snippet: .. To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table ) and a thermocycler (LightCycler 480, Roche Diagnostics). .. Expression levels were normalized to β-actin (Actb ) and relative expression was calculated using the comparative threshold cycle method (∆∆CT ) of the LightCycler480 software (Roche Diagnostics).

Article Title: UCHL1/PGP 9.5 Dynamic in Neuro-Immune-Cutaneous Milieu: Focusing on Axonal Nerve Terminals and Epidermal Keratinocytes in Psoriatic Itch
Article Snippet: Relative Gene Expression by Real-Time PCR (RT-PCR) The RT-PCR reaction was performed in Roche LightCycler 480 II Thermocycler (Roche, Germany) in a volume of 10 μ l. The reaction volume consisted of 5 μ l of LightCycler UPL ProbeMaster (Roche, Germany), 0,5 μ M forward and reverse primers, and 0.2 μ M UPL probe 27 and 1 μ l cDNA. .. The reaction conditions were as follows: preincubation at 95°C by 10 min., 50 cycles of amplification step: 95°C by 15 s for denaturation, 58°C by 1 min. for elongation step, and 10 s at 72°C for detection and next the plate was cooled at 40°C for 10 s. The relative gene expression was presented using 2-ΔΔCt method.

Article Title: Rapid screening for Klinefelter syndrome with a simple high-resolution melting assay: a multicenter study
Article Snippet: .. HRM assay PCR amplification and the HRM assay were performed on a LightCycler 480 II Thermocycler (Roche Applied Science GmbH, Mannheim, Germany). .. Each 25 μl reaction contained 10 mmol l−1 Tris-HCl (pH 8.3), 50 mmol l−1 KCl, 1 U TaqHS (Takara, Dalian, China), 2.5 mmol l−1 Mg2+ , 2 μmol l−1 SYTO™ 9 Green Fluorescent Nucleic Acid Stain (Molecular Probes, Eugene, OR, USA), 0.2 mmol l−1 of each deoxynucleoside triphosphate, and 0.2 μmol l−1 of each forward and reverse primer.

DNA Synthesis:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: .. The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche). .. The single strand DNA fragment thus obtained was purified from free dUTP-biotin by salt-precipitation and used as a probe for in situ hybridization on ultrathin sections.

Synthesized:

Article Title: An automated microfluidic device for assessment of mammalian cell genetic stability
Article Snippet: After the reverse transcription was completed, the sieve valves were relieved, and the beads with the synthesized cDNA were flushed to the beam-chambers. .. To minimize device expense and complexity, the temperature control and fluorescence detection were performed outside of the chip using commercial thermocycler (Roche LightCycler 480II).

Quantitative RT-PCR:

Article Title: M1 Macrophages Activate Notch Signalling in Epithelial Cells: Relevance in Crohn’s Disease
Article Snippet: Paragraph title: 2.12. RNA extraction and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analysis ... Real-time PCR was performed with the PrimeScript Reagent Kit Perfect Real Time (Takara) in a thermocycler (LightCycler, Roche Diagnostics).

SYBR Green Assay:

Article Title: Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells
Article Snippet: The resulting cDNA was subjected to real-time polymerase chain reaction (PCR) analysis by rapid cycling in glass capillaries with a thermocycler (Light-Cycler; Roche Molecular Biochemicals, Milano, Italy). .. The reaction was performed in a final volume of 20μl with Light-Cycler-DNA Master SYBR Green I (Roche Diagnostics, Milano, Italy), which contains nucleotides, Taq DNA polymerase, buffer, SYBR Green I dye and 10mM MgCl2; cDNA (2 μl), primers and sterile H2 O were added.

Article Title: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia
Article Snippet: .. To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table ) and a thermocycler (LightCycler 480, Roche Diagnostics). .. Expression levels were normalized to β-actin (Actb ) and relative expression was calculated using the comparative threshold cycle method (∆∆CT ) of the LightCycler480 software (Roche Diagnostics).

Article Title: Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: Relevance to age-related macular degeneration
Article Snippet: .. The PCR experiments were performed on a thermocycler (model LC 480 light cycler; RocheDiagnostics), with SYBR Green (Roche Diagnostics)as the interaction agent. .. Each 20 μL PCR mix contained 5 μLcDNA template, 10 μL SYBR Green PCR master mix, and 0.5μM of each gene-specific primer.

Article Title: CD8+ T cell-mediated endotheliopathy is a targetable mechanism of neuro-inflammation in Susac syndrome
Article Snippet: .. The cDNA prepared from mouse tissue was used as a template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA. ..

Article Title: Specific Activation of the Glucocorticoid Receptor and Modulation of Signal Transduction Pathways in Human Lens Epithelial Cells
Article Snippet: .. The primer sequences for DUSP-1/MKP-1and DSIP/GILZ have been published., PCR was performed with master mix (LightCycler FastStart DNA Master SYBR Green I kit; Roche Applied Science, Indianapolis, IN), and a thermocycler (LightCycler 1.0; Roche Applied Science), as previously described. ..

Article Title: Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound InflammationCancer Courts Immune Response to Aid Growth
Article Snippet: .. After 6 h, total RNA was extracted from pools of 60 larvae, and qPCR was performed with LightCycler 480 II thermocycler (Roche) using the SYBR Green I Master kit (Roche). ..

Article Title: CD8 T cell-mediated killing of orexinergic neurons induces a narcolepsy-like phenotype in mice
Article Snippet: .. The cDNA was used as template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA. .. Specific primers were used to amplify HA (forward, 5′-AAACTCTTCGCGGTCTTTCCA-3′; reverse, 5′-GATAAGGTAGCTTGGGCTGC-3′), orexin (forward, 5′-GACGACGGCCTCAGACTTCTTTG-3′; reverse, 5′-GAACCTTTGTAGAAGGAAGGTTCAT-3′), and HPRT (forward, 5′TGGTTAAGCAGTACAGCCCCAA-3′; reverse, 5′-AGGTCCTTTTCACCAGCAAGCT).

Microarray:

Article Title: The Effect of Acute and Chronic Social Stress on the Hippocampal Transcriptome in Mice
Article Snippet: .. Real—Time Quantitative PCR The microarray data were validated utilizing SYBR Green-based real-time quantitative PCR (qPCR) performed in 96-well plates on LightCycler® 480 II thermocycler (Roche, Germany). .. The hippocampal total RNA samples from the animals used in microarray study were individually analyzed (n = 9; 1 animal from control group for 8 days of stress was missing from analysis due to loss of sample).

Incubation:

Article Title: Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: Relevance to age-related macular degeneration
Article Snippet: Primary antibodies used were ZO-1 rabbit polyclonal antibody (1:1000 dilution; Zymed) and anti-occludin rabbit polyclonal antibody (1:500 dilution; Zymed).After incubation with horseradish peroxidase-conjugatedanti-rabbit secondary antibody (Vector Laboratories),protein bands were detected by chemiluminescence (Pierce). .. The PCR experiments were performed on a thermocycler (model LC 480 light cycler; RocheDiagnostics), with SYBR Green (Roche Diagnostics)as the interaction agent.

Expressing:

Article Title: Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells
Article Snippet: Paragraph title: IL-8, IL1-β, ICAM-1, iNOS, TNF-α, HBD1, HBD2, TLR2, TLR4 and MyD88 expression by Real-Time PCR ... The resulting cDNA was subjected to real-time polymerase chain reaction (PCR) analysis by rapid cycling in glass capillaries with a thermocycler (Light-Cycler; Roche Molecular Biochemicals, Milano, Italy).

Article Title: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia
Article Snippet: .. To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table ) and a thermocycler (LightCycler 480, Roche Diagnostics). .. Expression levels were normalized to β-actin (Actb ) and relative expression was calculated using the comparative threshold cycle method (∆∆CT ) of the LightCycler480 software (Roche Diagnostics).

Article Title: UCHL1/PGP 9.5 Dynamic in Neuro-Immune-Cutaneous Milieu: Focusing on Axonal Nerve Terminals and Epidermal Keratinocytes in Psoriatic Itch
Article Snippet: .. Relative Gene Expression by Real-Time PCR (RT-PCR) The RT-PCR reaction was performed in Roche LightCycler 480 II Thermocycler (Roche, Germany) in a volume of 10 μ l. The reaction volume consisted of 5 μ l of LightCycler UPL ProbeMaster (Roche, Germany), 0,5 μ M forward and reverse primers, and 0.2 μ M UPL probe 27 and 1 μ l cDNA. .. The reaction conditions were as follows: preincubation at 95°C by 10 min., 50 cycles of amplification step: 95°C by 15 s for denaturation, 58°C by 1 min. for elongation step, and 10 s at 72°C for detection and next the plate was cooled at 40°C for 10 s. The relative gene expression was presented using 2-ΔΔCt method.

Article Title: The Effect of Acute and Chronic Social Stress on the Hippocampal Transcriptome in Mice
Article Snippet: Real—Time Quantitative PCR The microarray data were validated utilizing SYBR Green-based real-time quantitative PCR (qPCR) performed in 96-well plates on LightCycler® 480 II thermocycler (Roche, Germany). .. The expression levels of the following genes were studied: Ttr , Aqp1 , Prlr , Kcne2 , Folr1 , Spp1 , Egr2 , S100a8 , Vwf , and Gbp4 .

Article Title: CD8+ T cell-mediated endotheliopathy is a targetable mechanism of neuro-inflammation in Susac syndrome
Article Snippet: .. The cDNA prepared from mouse tissue was used as a template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA. ..

Article Title: CD8 T cell-mediated killing of orexinergic neurons induces a narcolepsy-like phenotype in mice
Article Snippet: .. The cDNA was used as template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA. .. Specific primers were used to amplify HA (forward, 5′-AAACTCTTCGCGGTCTTTCCA-3′; reverse, 5′-GATAAGGTAGCTTGGGCTGC-3′), orexin (forward, 5′-GACGACGGCCTCAGACTTCTTTG-3′; reverse, 5′-GAACCTTTGTAGAAGGAAGGTTCAT-3′), and HPRT (forward, 5′TGGTTAAGCAGTACAGCCCCAA-3′; reverse, 5′-AGGTCCTTTTCACCAGCAAGCT).

Filtration:

Article Title: Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission
Article Snippet: The protein solution (4 mg/ml) was incubated with 1 ml of LIPIDEX 1000 matrix (Perkin Elmer) previously equilibrated in the gel filtration buffer, for 1 hour at 37°C under gentle agitation. .. The plates were heated from 25°C to 85°C with a ramp rate of 0.07°C/s and read by a thermocycler (LightCycler 480 II, Roche) using excitation and emission wavelengths of 465 nm and 510 nm, respectively.

Sequencing:

Article Title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys
Article Snippet: .. According to Roche/454 protocols, emulsion PCR (emPCR) was then performed on the pool with thermocycler conditions: 94°C for 4 min and then 50 cycles of 94°C for 30 sec, 58°C for 4.5 min, 68°C for 30 sec. emPCR was followed by breaking, enriching, and sequencing on either the Roche/454 GS FLX or GS Junior instrument using Titanium technology. ..

Nick Translation:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: Labeling with biotin was performed by nick translation on the entire plasmids in the presence of dUTP-16-biotin (Enzo). .. The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche).

Generated:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche). .. An antisense probe to the ITS2 was generated by in vitro transcription of the corresponding DNA fragment subcloned into pGEM4.

Reverse Transcription Polymerase Chain Reaction:

Article Title: M1 Macrophages Activate Notch Signalling in Epithelial Cells: Relevance in Crohn’s Disease
Article Snippet: Paragraph title: 2.12. RNA extraction and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analysis ... Real-time PCR was performed with the PrimeScript Reagent Kit Perfect Real Time (Takara) in a thermocycler (LightCycler, Roche Diagnostics).

Article Title: UCHL1/PGP 9.5 Dynamic in Neuro-Immune-Cutaneous Milieu: Focusing on Axonal Nerve Terminals and Epidermal Keratinocytes in Psoriatic Itch
Article Snippet: .. Relative Gene Expression by Real-Time PCR (RT-PCR) The RT-PCR reaction was performed in Roche LightCycler 480 II Thermocycler (Roche, Germany) in a volume of 10 μ l. The reaction volume consisted of 5 μ l of LightCycler UPL ProbeMaster (Roche, Germany), 0,5 μ M forward and reverse primers, and 0.2 μ M UPL probe 27 and 1 μ l cDNA. .. The reaction conditions were as follows: preincubation at 95°C by 10 min., 50 cycles of amplification step: 95°C by 15 s for denaturation, 58°C by 1 min. for elongation step, and 10 s at 72°C for detection and next the plate was cooled at 40°C for 10 s. The relative gene expression was presented using 2-ΔΔCt method.

Article Title: An automated microfluidic device for assessment of mammalian cell genetic stability
Article Snippet: The COC plate was put on a homemade fixture to be inserted to the RT-PCR equipment. .. To minimize device expense and complexity, the temperature control and fluorescence detection were performed outside of the chip using commercial thermocycler (Roche LightCycler 480II).

Article Title: Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound InflammationCancer Courts Immune Response to Aid Growth
Article Snippet: Paragraph title: Morphotyping, RT-PCR, and qPCR ... After 6 h, total RNA was extracted from pools of 60 larvae, and qPCR was performed with LightCycler 480 II thermocycler (Roche) using the SYBR Green I Master kit (Roche).

Article Title: CD8 T cell-mediated killing of orexinergic neurons induces a narcolepsy-like phenotype in mice
Article Snippet: Paragraph title: RT-PCR. ... The cDNA was used as template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA.

Fluorescence:

Article Title: An automated microfluidic device for assessment of mammalian cell genetic stability
Article Snippet: .. To minimize device expense and complexity, the temperature control and fluorescence detection were performed outside of the chip using commercial thermocycler (Roche LightCycler 480II). ..

Article Title: Rapid screening for Klinefelter syndrome with a simple high-resolution melting assay: a multicenter study
Article Snippet: HRM assay PCR amplification and the HRM assay were performed on a LightCycler 480 II Thermocycler (Roche Applied Science GmbH, Mannheim, Germany). .. The reaction conditions were as follows: 95°C for 3 min followed by 40 cycles of 95°C for 15 s, 60°C for 15 s, and 72°C for 15 s. The HRM analysis began with a denaturation step at 95°C for 1 min and a renaturation step at 40°C for 1 min, followed by melting (70°C–85°C, with a 0.03°C s−1 ramp rate), and 20 fluorescence acquisitions per °C were collected.

HRM Assay:

Article Title: Rapid screening for Klinefelter syndrome with a simple high-resolution melting assay: a multicenter study
Article Snippet: .. HRM assay PCR amplification and the HRM assay were performed on a LightCycler 480 II Thermocycler (Roche Applied Science GmbH, Mannheim, Germany). .. Each 25 μl reaction contained 10 mmol l−1 Tris-HCl (pH 8.3), 50 mmol l−1 KCl, 1 U TaqHS (Takara, Dalian, China), 2.5 mmol l−1 Mg2+ , 2 μmol l−1 SYTO™ 9 Green Fluorescent Nucleic Acid Stain (Molecular Probes, Eugene, OR, USA), 0.2 mmol l−1 of each deoxynucleoside triphosphate, and 0.2 μmol l−1 of each forward and reverse primer.

Isolation:

Article Title: Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells
Article Snippet: IL-8, IL1-β, ICAM-1, iNOS, TNF-α, HBD1, HBD2, TLR2, TLR4 and MyD88 expression by Real-Time PCR Total RNA isolated by High Pure RNA Isolation Kit (Roche Diagnostics, Milan, Italy) from Caco-2 cells before and after treatment with LPS and porins for 3, 6 and 24 h was transcribed by reverse transcriptase (Expand Reverse Transcriptase, Roche) at 42°C for 45 min according to the manufacturer’s protocol. .. The resulting cDNA was subjected to real-time polymerase chain reaction (PCR) analysis by rapid cycling in glass capillaries with a thermocycler (Light-Cycler; Roche Molecular Biochemicals, Milano, Italy).

Article Title: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia
Article Snippet: Paragraph title: RNA isolation and semi-quantitative real-time PCR ... To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table ) and a thermocycler (LightCycler 480, Roche Diagnostics).

Article Title: Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: Relevance to age-related macular degeneration
Article Snippet: Total RNA was isolated (TRIzol extraction protocol; Invitrogen), and treated with DNase (Ambion)to remove contaminating genomic DNA. .. The PCR experiments were performed on a thermocycler (model LC 480 light cycler; RocheDiagnostics), with SYBR Green (Roche Diagnostics)as the interaction agent.

Article Title: CD8+ T cell-mediated endotheliopathy is a targetable mechanism of neuro-inflammation in Susac syndrome
Article Snippet: Paragraph title: RNA isolation and reverse transcriptase-PCR ... The cDNA prepared from mouse tissue was used as a template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA.

Article Title: Specific Activation of the Glucocorticoid Receptor and Modulation of Signal Transduction Pathways in Human Lens Epithelial Cells
Article Snippet: Total RNA was isolated (RNAzol; Tel-Test) according to the manufacturer’s protocol. .. The primer sequences for DUSP-1/MKP-1and DSIP/GILZ have been published., PCR was performed with master mix (LightCycler FastStart DNA Master SYBR Green I kit; Roche Applied Science, Indianapolis, IN), and a thermocycler (LightCycler 1.0; Roche Applied Science), as previously described.

Size-exclusion Chromatography:

Article Title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys
Article Snippet: .. According to Roche/454 protocols, emulsion PCR (emPCR) was then performed on the pool with thermocycler conditions: 94°C for 4 min and then 50 cycles of 94°C for 30 sec, 58°C for 4.5 min, 68°C for 30 sec. emPCR was followed by breaking, enriching, and sequencing on either the Roche/454 GS FLX or GS Junior instrument using Titanium technology. ..

Labeling:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: Labeling with biotin was performed by nick translation on the entire plasmids in the presence of dUTP-16-biotin (Enzo). .. The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche).

Mouse Assay:

Article Title: CD8+ T cell-mediated endotheliopathy is a targetable mechanism of neuro-inflammation in Susac syndrome
Article Snippet: To assess HA gene expression in mice, total RNA was extracted from mouse tissue with the RNeasy Mini-kit or RNeasy Micro-kit plus (Qiagen), followed by reverse transcription using RevertAidH Minus Revert Transcriptase (ThermoFisher). .. The cDNA prepared from mouse tissue was used as a template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA.

Polymerase Chain Reaction:

Article Title: Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells
Article Snippet: .. The resulting cDNA was subjected to real-time polymerase chain reaction (PCR) analysis by rapid cycling in glass capillaries with a thermocycler (Light-Cycler; Roche Molecular Biochemicals, Milano, Italy). .. The reaction was performed in a final volume of 20μl with Light-Cycler-DNA Master SYBR Green I (Roche Diagnostics, Milano, Italy), which contains nucleotides, Taq DNA polymerase, buffer, SYBR Green I dye and 10mM MgCl2; cDNA (2 μl), primers and sterile H2 O were added.

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: To obtain a probe complementary to the 5′ part of the ITS1, the D-A2 rDNA fragment from S. cerevisiae was amplified by PCR and subcloned into pGEM4. .. The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche).

Article Title: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia
Article Snippet: .. To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table ) and a thermocycler (LightCycler 480, Roche Diagnostics). .. Expression levels were normalized to β-actin (Actb ) and relative expression was calculated using the comparative threshold cycle method (∆∆CT ) of the LightCycler480 software (Roche Diagnostics).

Article Title: Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: Relevance to age-related macular degeneration
Article Snippet: .. The PCR experiments were performed on a thermocycler (model LC 480 light cycler; RocheDiagnostics), with SYBR Green (Roche Diagnostics)as the interaction agent. .. Each 20 μL PCR mix contained 5 μLcDNA template, 10 μL SYBR Green PCR master mix, and 0.5μM of each gene-specific primer.

Article Title: An automated microfluidic device for assessment of mammalian cell genetic stability
Article Snippet: After peeling off the PDMS device, the 32 wells were loaded with PCR mixture and sealed with an adhesive PCR sealant film. .. To minimize device expense and complexity, the temperature control and fluorescence detection were performed outside of the chip using commercial thermocycler (Roche LightCycler 480II).

Article Title: Rapid screening for Klinefelter syndrome with a simple high-resolution melting assay: a multicenter study
Article Snippet: .. HRM assay PCR amplification and the HRM assay were performed on a LightCycler 480 II Thermocycler (Roche Applied Science GmbH, Mannheim, Germany). .. Each 25 μl reaction contained 10 mmol l−1 Tris-HCl (pH 8.3), 50 mmol l−1 KCl, 1 U TaqHS (Takara, Dalian, China), 2.5 mmol l−1 Mg2+ , 2 μmol l−1 SYTO™ 9 Green Fluorescent Nucleic Acid Stain (Molecular Probes, Eugene, OR, USA), 0.2 mmol l−1 of each deoxynucleoside triphosphate, and 0.2 μmol l−1 of each forward and reverse primer.

Article Title: Specific Activation of the Glucocorticoid Receptor and Modulation of Signal Transduction Pathways in Human Lens Epithelial Cells
Article Snippet: .. The primer sequences for DUSP-1/MKP-1and DSIP/GILZ have been published., PCR was performed with master mix (LightCycler FastStart DNA Master SYBR Green I kit; Roche Applied Science, Indianapolis, IN), and a thermocycler (LightCycler 1.0; Roche Applied Science), as previously described. ..

Article Title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys
Article Snippet: .. According to Roche/454 protocols, emulsion PCR (emPCR) was then performed on the pool with thermocycler conditions: 94°C for 4 min and then 50 cycles of 94°C for 30 sec, 58°C for 4.5 min, 68°C for 30 sec. emPCR was followed by breaking, enriching, and sequencing on either the Roche/454 GS FLX or GS Junior instrument using Titanium technology. ..

Purification:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche). .. The single strand DNA fragment thus obtained was purified from free dUTP-biotin by salt-precipitation and used as a probe for in situ hybridization on ultrathin sections.

Article Title: Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission
Article Snippet: The delipidation treatment of the purified protein was performed as described in [ ]. .. The plates were heated from 25°C to 85°C with a ramp rate of 0.07°C/s and read by a thermocycler (LightCycler 480 II, Roche) using excitation and emission wavelengths of 465 nm and 510 nm, respectively.

Chromatin Immunoprecipitation:

Article Title: An automated microfluidic device for assessment of mammalian cell genetic stability
Article Snippet: .. To minimize device expense and complexity, the temperature control and fluorescence detection were performed outside of the chip using commercial thermocycler (Roche LightCycler 480II). ..

In Situ Hybridization:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: Paragraph title: Probes for ultrastructural in situ hybridization ... The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche).

Plasmid Preparation:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: .. The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche). .. The single strand DNA fragment thus obtained was purified from free dUTP-biotin by salt-precipitation and used as a probe for in situ hybridization on ultrathin sections.

Software:

Article Title: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia
Article Snippet: To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table ) and a thermocycler (LightCycler 480, Roche Diagnostics). .. Expression levels were normalized to β-actin (Actb ) and relative expression was calculated using the comparative threshold cycle method (∆∆CT ) of the LightCycler480 software (Roche Diagnostics).

Article Title: Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission
Article Snippet: The plates were heated from 25°C to 85°C with a ramp rate of 0.07°C/s and read by a thermocycler (LightCycler 480 II, Roche) using excitation and emission wavelengths of 465 nm and 510 nm, respectively. .. The Tms were determined using the LightCycler480 Software.

Real-time Polymerase Chain Reaction:

Article Title: Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells
Article Snippet: .. The resulting cDNA was subjected to real-time polymerase chain reaction (PCR) analysis by rapid cycling in glass capillaries with a thermocycler (Light-Cycler; Roche Molecular Biochemicals, Milano, Italy). .. The reaction was performed in a final volume of 20μl with Light-Cycler-DNA Master SYBR Green I (Roche Diagnostics, Milano, Italy), which contains nucleotides, Taq DNA polymerase, buffer, SYBR Green I dye and 10mM MgCl2; cDNA (2 μl), primers and sterile H2 O were added.

Article Title: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia
Article Snippet: .. To analyze gene expression by real-time PCR, 10 ng of cDNA template was amplified using a PCR polymerase ready mix (LightCycler 480 SYBR Green I Master, Roche Diagnostics, Rotkreuz, Switzerland), specific primer pairs (Table ) and a thermocycler (LightCycler 480, Roche Diagnostics). .. Expression levels were normalized to β-actin (Actb ) and relative expression was calculated using the comparative threshold cycle method (∆∆CT ) of the LightCycler480 software (Roche Diagnostics).

Article Title: M1 Macrophages Activate Notch Signalling in Epithelial Cells: Relevance in Crohn’s Disease
Article Snippet: .. Real-time PCR was performed with the PrimeScript Reagent Kit Perfect Real Time (Takara) in a thermocycler (LightCycler, Roche Diagnostics). .. A ChIP assay was carried out in U937-derived macrophages, incubated under hypoxia or normoxia for 5h, as previously described.

Article Title: UCHL1/PGP 9.5 Dynamic in Neuro-Immune-Cutaneous Milieu: Focusing on Axonal Nerve Terminals and Epidermal Keratinocytes in Psoriatic Itch
Article Snippet: .. Relative Gene Expression by Real-Time PCR (RT-PCR) The RT-PCR reaction was performed in Roche LightCycler 480 II Thermocycler (Roche, Germany) in a volume of 10 μ l. The reaction volume consisted of 5 μ l of LightCycler UPL ProbeMaster (Roche, Germany), 0,5 μ M forward and reverse primers, and 0.2 μ M UPL probe 27 and 1 μ l cDNA. .. The reaction conditions were as follows: preincubation at 95°C by 10 min., 50 cycles of amplification step: 95°C by 15 s for denaturation, 58°C by 1 min. for elongation step, and 10 s at 72°C for detection and next the plate was cooled at 40°C for 10 s. The relative gene expression was presented using 2-ΔΔCt method.

Article Title: The Effect of Acute and Chronic Social Stress on the Hippocampal Transcriptome in Mice
Article Snippet: .. Real—Time Quantitative PCR The microarray data were validated utilizing SYBR Green-based real-time quantitative PCR (qPCR) performed in 96-well plates on LightCycler® 480 II thermocycler (Roche, Germany). .. The hippocampal total RNA samples from the animals used in microarray study were individually analyzed (n = 9; 1 animal from control group for 8 days of stress was missing from analysis due to loss of sample).

Article Title: CD8+ T cell-mediated endotheliopathy is a targetable mechanism of neuro-inflammation in Susac syndrome
Article Snippet: .. The cDNA prepared from mouse tissue was used as a template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA. ..

Article Title: Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound InflammationCancer Courts Immune Response to Aid Growth
Article Snippet: .. After 6 h, total RNA was extracted from pools of 60 larvae, and qPCR was performed with LightCycler 480 II thermocycler (Roche) using the SYBR Green I Master kit (Roche). ..

Article Title: CD8 T cell-mediated killing of orexinergic neurons induces a narcolepsy-like phenotype in mice
Article Snippet: .. The cDNA was used as template for quantitative PCR using SYBR Green I master mix (Roche) and assessed in a thermocycler (LightCycler 480; Roche). mRNA expression was normalized to that of HPRT mRNA. .. Specific primers were used to amplify HA (forward, 5′-AAACTCTTCGCGGTCTTTCCA-3′; reverse, 5′-GATAAGGTAGCTTGGGCTGC-3′), orexin (forward, 5′-GACGACGGCCTCAGACTTCTTTG-3′; reverse, 5′-GAACCTTTGTAGAAGGAAGGTTCAT-3′), and HPRT (forward, 5′TGGTTAAGCAGTACAGCCCCAA-3′; reverse, 5′-AGGTCCTTTTCACCAGCAAGCT).

RNA Extraction:

Article Title: M1 Macrophages Activate Notch Signalling in Epithelial Cells: Relevance in Crohn’s Disease
Article Snippet: Paragraph title: 2.12. RNA extraction and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analysis ... Real-time PCR was performed with the PrimeScript Reagent Kit Perfect Real Time (Takara) in a thermocycler (LightCycler, Roche Diagnostics).

Agarose Gel Electrophoresis:

Article Title: Attainment of polarity promotes growth factor secretion by retinal pigment epithelial cells: Relevance to age-related macular degeneration
Article Snippet: The PCR experiments were performed on a thermocycler (model LC 480 light cycler; RocheDiagnostics), with SYBR Green (Roche Diagnostics)as the interaction agent. .. The specificity of the PCR amplificationproducts was checked by performing dissociation melting curveanalysis and by 1% agarose gel electrophoresis.

In Vitro:

Article Title: Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex
Article Snippet: The resulting plasmid was linearized at a restriction site close to site D, and used for multiple cycles of DNA synthesis in a thermocycler using Goldstar DNA polymerase (Roche), primer 5′-CTC CAC AGT GTG TTG TAT TG, which encompasses site A2, and a mix of dNTPs containing dUTP-biotin (Roche). .. An antisense probe to the ITS2 was generated by in vitro transcription of the corresponding DNA fragment subcloned into pGEM4.

Produced:

Article Title: Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound InflammationCancer Courts Immune Response to Aid Growth
Article Snippet: DUOX-morpholino-mediated splice perturbation produced a 39-bp in-frame deletion within the peroxidase-like domain of DUOX as described before ( ) . .. After 6 h, total RNA was extracted from pools of 60 larvae, and qPCR was performed with LightCycler 480 II thermocycler (Roche) using the SYBR Green I Master kit (Roche).

Staining:

Article Title: Rapid screening for Klinefelter syndrome with a simple high-resolution melting assay: a multicenter study
Article Snippet: HRM assay PCR amplification and the HRM assay were performed on a LightCycler 480 II Thermocycler (Roche Applied Science GmbH, Mannheim, Germany). .. Each 25 μl reaction contained 10 mmol l−1 Tris-HCl (pH 8.3), 50 mmol l−1 KCl, 1 U TaqHS (Takara, Dalian, China), 2.5 mmol l−1 Mg2+ , 2 μmol l−1 SYTO™ 9 Green Fluorescent Nucleic Acid Stain (Molecular Probes, Eugene, OR, USA), 0.2 mmol l−1 of each deoxynucleoside triphosphate, and 0.2 μmol l−1 of each forward and reverse primer.

Article Title: Specific Activation of the Glucocorticoid Receptor and Modulation of Signal Transduction Pathways in Human Lens Epithelial Cells
Article Snippet: The primer sequences for DUSP-1/MKP-1and DSIP/GILZ have been published., PCR was performed with master mix (LightCycler FastStart DNA Master SYBR Green I kit; Roche Applied Science, Indianapolis, IN), and a thermocycler (LightCycler 1.0; Roche Applied Science), as previously described. .. The specificity of PCR products was determined by melting-curve analysis and visualization on 1% or 2% agarose gels stained with ethidium bromide.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 77
    Roche fluorescent based thermocycler
    Clinical sample processing and analysis. (A) S. aureus detection by EBT-color change from purple to blue-Tubes 1 and 2, negative, and Tubes 3 and 4, positive. (B) Positives, S aureus clinical sample amplification by <t>thermocycler</t> (first, pink tracing) and positive controls, S aureus amplification (second, blue tracing). (C) Cuvettes with positive (left) and negative (right) color change corresponding to spiked DNA samples. (D) Spectrophotometric analysis at 420 nm with corresponding positive control amplification (blue) and negative control (orange) at times 0–45 min for positive and negative samples ( n = 3).
    Fluorescent Based Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent based thermocycler/product/Roche
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent based thermocycler - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    83
    Roche thermocyclers
    Threshold cycle variation when using different reaction conditions and <t>thermocyclers</t>
    Thermocyclers, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocyclers/product/Roche
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    thermocyclers - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    90
    Roche roche 480 thermocycler
    Threshold cycle variation when using different reaction conditions and <t>thermocyclers</t>
    Roche 480 Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/roche 480 thermocycler/product/Roche
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    roche 480 thermocycler - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    87
    Roche roche lc480 thermocycler
    Threshold cycle variation when using different reaction conditions and <t>thermocyclers</t>
    Roche Lc480 Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/roche lc480 thermocycler/product/Roche
    Average 87 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    roche lc480 thermocycler - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    Image Search Results


    Clinical sample processing and analysis. (A) S. aureus detection by EBT-color change from purple to blue-Tubes 1 and 2, negative, and Tubes 3 and 4, positive. (B) Positives, S aureus clinical sample amplification by thermocycler (first, pink tracing) and positive controls, S aureus amplification (second, blue tracing). (C) Cuvettes with positive (left) and negative (right) color change corresponding to spiked DNA samples. (D) Spectrophotometric analysis at 420 nm with corresponding positive control amplification (blue) and negative control (orange) at times 0–45 min for positive and negative samples ( n = 3).

    Journal: Frontiers in Microbiology

    Article Title: Evaluation of Nucleic Acid Isothermal Amplification Methods for Human Clinical Microbial Infection Detection

    doi: 10.3389/fmicb.2017.02211

    Figure Lengend Snippet: Clinical sample processing and analysis. (A) S. aureus detection by EBT-color change from purple to blue-Tubes 1 and 2, negative, and Tubes 3 and 4, positive. (B) Positives, S aureus clinical sample amplification by thermocycler (first, pink tracing) and positive controls, S aureus amplification (second, blue tracing). (C) Cuvettes with positive (left) and negative (right) color change corresponding to spiked DNA samples. (D) Spectrophotometric analysis at 420 nm with corresponding positive control amplification (blue) and negative control (orange) at times 0–45 min for positive and negative samples ( n = 3).

    Article Snippet: In Step 1, clinical pathogen concentrations were estimated using both a fluorescent-based thermocycler unit (Roche LightCycler 96) and a parallel visual discrimination test utilizing EBT Dye-based reaction color change.

    Techniques: Amplification, Positive Control, Negative Control

    Threshold cycle variation when using different reaction conditions and thermocyclers

    Journal: Bulletin of the World Health Organization

    Article Title: Assay optimization for molecular detection of Zika virus

    doi: 10.2471/BLT.16.175950

    Figure Lengend Snippet: Threshold cycle variation when using different reaction conditions and thermocyclers

    Article Snippet: Dengue virus RNA quantification and flavivirus typing were done as described previously., For all of the experiments, except when assessing threshold cycle variation using different reaction conditions and thermocyclers, we quantified Zika virus RNA using the LightCycler® 480 Instrument II (Roche, Basel, Switzerland).

    Techniques: