thermocycler  (Roche)


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  • 92

    Structured Review

    Roche thermocycler
    Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Roche
    Average 92 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-09
    92/100 stars

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    Related Articles

    Amplification:

    Article Title: Differential Modulation of prM Cleavage, Extracellular Particle Distribution, and Virus Infectivity by Conserved Residues at Nonfurin Consensus Positions of the Dengue Virus pr-M Junction ▿
    Article Snippet: .. In this study, the RT-PCR was performed in the capillary tube format using a thermocycler with a real-time display of the amplification results (Roche, Mannheim, Germany), and the specific infectivity was expressed as FFU/1,000 genomic RNA copies. .. A comparison of infectivity was performed between C6/36 and PS (C6/36-PS) cells and PS and PS (PS-PS) cells, according to the cellular sources employed in virus preparation and the quantitation of infectious virus titer, respectively.

    In Vitro:

    Article Title: Midgut aminopeptidase N expression profile in Castor semilooper during sublethal Cry toxin exposure
    Article Snippet: .. In vitro transcription of DNA template was carried out using thermocycler followed by RNA probe synthesis using DIG RNA labelling kit using manufactures protocol (Roche Diagnostics, Germany). .. DIG labelled mRNA was synthesized and analysed using agarose gel electrophoresis and sequencing.

    Size-exclusion Chromatography:

    Article Title: Androgen receptor-mediated upregulation of quaking affects androgen receptor-related prostate cancer development and anti-androgen receptor therapy
    Article Snippet: .. The RT reaction was as follows: 37°C for 15 min and 85°C for 5 sec. qPCR analyses were performed using the SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.); the thermocycler used for AR, QKI and PSA mRNA analysis was the LightCycler 480 system (Roche, Basel, Switzerland) and the thermocycler used for the analysis of all other mRNAs was the Applied Biosystems® 7500 Fast Real-time PCR system (Thermo Fisher Scientific, Inc.). .. The thermal cycling conditions were as follows: 95°C for 10 min, followed by 40 cycles at 95°C for 10 sec, 60°C for 35 sec and 72°C for 45 sec, followed by a final elongation step at 72°C for 10 min.

    Article Title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys
    Article Snippet: .. According to Roche/454 protocols, emulsion PCR (emPCR) was then performed on the pool with thermocycler conditions: 94°C for 4 min and then 50 cycles of 94°C for 30 sec, 58°C for 4.5 min, 68°C for 30 sec. emPCR was followed by breaking, enriching, and sequencing on either the Roche/454 GS FLX or GS Junior instrument using Titanium technology. ..

    Infection:

    Article Title: Differential Modulation of prM Cleavage, Extracellular Particle Distribution, and Virus Infectivity by Conserved Residues at Nonfurin Consensus Positions of the Dengue Virus pr-M Junction ▿
    Article Snippet: .. In this study, the RT-PCR was performed in the capillary tube format using a thermocycler with a real-time display of the amplification results (Roche, Mannheim, Germany), and the specific infectivity was expressed as FFU/1,000 genomic RNA copies. .. A comparison of infectivity was performed between C6/36 and PS (C6/36-PS) cells and PS and PS (PS-PS) cells, according to the cellular sources employed in virus preparation and the quantitation of infectious virus titer, respectively.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Differential Modulation of prM Cleavage, Extracellular Particle Distribution, and Virus Infectivity by Conserved Residues at Nonfurin Consensus Positions of the Dengue Virus pr-M Junction ▿
    Article Snippet: .. In this study, the RT-PCR was performed in the capillary tube format using a thermocycler with a real-time display of the amplification results (Roche, Mannheim, Germany), and the specific infectivity was expressed as FFU/1,000 genomic RNA copies. .. A comparison of infectivity was performed between C6/36 and PS (C6/36-PS) cells and PS and PS (PS-PS) cells, according to the cellular sources employed in virus preparation and the quantitation of infectious virus titer, respectively.

    Real-time Polymerase Chain Reaction:

    Article Title: Androgen receptor-mediated upregulation of quaking affects androgen receptor-related prostate cancer development and anti-androgen receptor therapy
    Article Snippet: .. The RT reaction was as follows: 37°C for 15 min and 85°C for 5 sec. qPCR analyses were performed using the SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.); the thermocycler used for AR, QKI and PSA mRNA analysis was the LightCycler 480 system (Roche, Basel, Switzerland) and the thermocycler used for the analysis of all other mRNAs was the Applied Biosystems® 7500 Fast Real-time PCR system (Thermo Fisher Scientific, Inc.). .. The thermal cycling conditions were as follows: 95°C for 10 min, followed by 40 cycles at 95°C for 10 sec, 60°C for 35 sec and 72°C for 45 sec, followed by a final elongation step at 72°C for 10 min.

    Polymerase Chain Reaction:

    Article Title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys
    Article Snippet: .. According to Roche/454 protocols, emulsion PCR (emPCR) was then performed on the pool with thermocycler conditions: 94°C for 4 min and then 50 cycles of 94°C for 30 sec, 58°C for 4.5 min, 68°C for 30 sec. emPCR was followed by breaking, enriching, and sequencing on either the Roche/454 GS FLX or GS Junior instrument using Titanium technology. ..

    Sequencing:

    Article Title: Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys
    Article Snippet: .. According to Roche/454 protocols, emulsion PCR (emPCR) was then performed on the pool with thermocycler conditions: 94°C for 4 min and then 50 cycles of 94°C for 30 sec, 58°C for 4.5 min, 68°C for 30 sec. emPCR was followed by breaking, enriching, and sequencing on either the Roche/454 GS FLX or GS Junior instrument using Titanium technology. ..

    Software:

    Article Title: Size Alters Susceptibility of Vectors to Dengue Virus Infection and Dissemination
    Article Snippet: .. Glass capillary tubes, containing reaction reagents, were placed in a thermocycler, LightCycler 1.2 instrument, equipped with LightCycler software version 3.5 (Roche Molecular Biochemicals, Indianapolis, IN). .. Ninety-six-well reaction plates were placed in a thermocycler, LC480 (Roche Molecular Biochemicals).

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  • 90
    Roche thermocyclers
    Comparison of quantitative cycle ( Cq ) values independently of mixes and <t>thermocyclers</t> between qPCR method targeting WNA or DNA for high (pure extract, HFL) and low (1:1000 dilution, LFL) fungal load. WNA: whole nucleic acid; Cq : quantitative cycle. *** p
    Thermocyclers, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocyclers/product/Roche
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermocyclers - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    91
    Roche fluorescent based thermocycler
    Clinical sample processing and analysis. (A) S. aureus detection by EBT-color change from purple to blue-Tubes 1 and 2, negative, and Tubes 3 and 4, positive. (B) Positives, S aureus clinical sample amplification by <t>thermocycler</t> (first, pink tracing) and positive controls, S aureus amplification (second, blue tracing). (C) Cuvettes with positive (left) and negative (right) color change corresponding to spiked DNA samples. (D) Spectrophotometric analysis at 420 nm with corresponding positive control amplification (blue) and negative control (orange) at times 0–45 min for positive and negative samples ( n = 3).
    Fluorescent Based Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent based thermocycler/product/Roche
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent based thermocycler - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    Roche other portable thermocyclers
    Clinical sample processing and analysis. (A) S. aureus detection by EBT-color change from purple to blue-Tubes 1 and 2, negative, and Tubes 3 and 4, positive. (B) Positives, S aureus clinical sample amplification by <t>thermocycler</t> (first, pink tracing) and positive controls, S aureus amplification (second, blue tracing). (C) Cuvettes with positive (left) and negative (right) color change corresponding to spiked DNA samples. (D) Spectrophotometric analysis at 420 nm with corresponding positive control amplification (blue) and negative control (orange) at times 0–45 min for positive and negative samples ( n = 3).
    Other Portable Thermocyclers, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/other portable thermocyclers/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    other portable thermocyclers - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of quantitative cycle ( Cq ) values independently of mixes and thermocyclers between qPCR method targeting WNA or DNA for high (pure extract, HFL) and low (1:1000 dilution, LFL) fungal load. WNA: whole nucleic acid; Cq : quantitative cycle. *** p

    Journal: Journal of Fungi

    Article Title: Quantification of Pneumocystis jirovecii: Cross-Platform Comparison of One qPCR Assay with Leading Platforms and Six Master Mixes

    doi: 10.3390/jof6010009

    Figure Lengend Snippet: Comparison of quantitative cycle ( Cq ) values independently of mixes and thermocyclers between qPCR method targeting WNA or DNA for high (pure extract, HFL) and low (1:1000 dilution, LFL) fungal load. WNA: whole nucleic acid; Cq : quantitative cycle. *** p

    Article Snippet: Experiments were performed on four thermocyclers (Roche LightCycler 480, Qiagen Rotor-Gene Q, Applied Biosystem ABI7500, and QuantStudio).

    Techniques: Real-time Polymerase Chain Reaction

    Threshold cycle variation when using different reaction conditions and thermocyclers

    Journal: Bulletin of the World Health Organization

    Article Title: Assay optimization for molecular detection of Zika virus

    doi: 10.2471/BLT.16.175950

    Figure Lengend Snippet: Threshold cycle variation when using different reaction conditions and thermocyclers

    Article Snippet: Dengue virus RNA quantification and flavivirus typing were done as described previously., For all of the experiments, except when assessing threshold cycle variation using different reaction conditions and thermocyclers, we quantified Zika virus RNA using the LightCycler® 480 Instrument II (Roche, Basel, Switzerland).

    Techniques:

    Clinical sample processing and analysis. (A) S. aureus detection by EBT-color change from purple to blue-Tubes 1 and 2, negative, and Tubes 3 and 4, positive. (B) Positives, S aureus clinical sample amplification by thermocycler (first, pink tracing) and positive controls, S aureus amplification (second, blue tracing). (C) Cuvettes with positive (left) and negative (right) color change corresponding to spiked DNA samples. (D) Spectrophotometric analysis at 420 nm with corresponding positive control amplification (blue) and negative control (orange) at times 0–45 min for positive and negative samples ( n = 3).

    Journal: Frontiers in Microbiology

    Article Title: Evaluation of Nucleic Acid Isothermal Amplification Methods for Human Clinical Microbial Infection Detection

    doi: 10.3389/fmicb.2017.02211

    Figure Lengend Snippet: Clinical sample processing and analysis. (A) S. aureus detection by EBT-color change from purple to blue-Tubes 1 and 2, negative, and Tubes 3 and 4, positive. (B) Positives, S aureus clinical sample amplification by thermocycler (first, pink tracing) and positive controls, S aureus amplification (second, blue tracing). (C) Cuvettes with positive (left) and negative (right) color change corresponding to spiked DNA samples. (D) Spectrophotometric analysis at 420 nm with corresponding positive control amplification (blue) and negative control (orange) at times 0–45 min for positive and negative samples ( n = 3).

    Article Snippet: In Step 1, clinical pathogen concentrations were estimated using both a fluorescent-based thermocycler unit (Roche LightCycler 96) and a parallel visual discrimination test utilizing EBT Dye-based reaction color change.

    Techniques: Amplification, Positive Control, Negative Control