Structured Review

Bio-Rad thermocycler
Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thermocycler/product/Bio-Rad
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
thermocycler - by Bioz Stars, 2020-02
99/100 stars

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Amplification:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: MJ Mini Thermal Cycler (Bio-Rad) was used for all reactions and amplification products were analyzed on 1.8% agarose gel stained with ethidium bromide. .. For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′.

Article Title: Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway
Article Snippet: The amplified cDNA was utilized for the template DNA and performed with specific primers for PCR assay. .. The RT reaction was performed in thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) at 42°C for 2 min, 37°C for 15 min, and 85°C for 5 sec.

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California). .. To verify amplification of the target gene, PCR products were purified (Centricon-100; Millipore, Billerica, Massachusetts) and directly sequenced at the University of Chicago Cancer Research Center DNA Sequencing Facility.

Article Title: Synthesizing and Salvaging NAD+: Lessons Learned from Chlamydomonas reinhardtii
Article Snippet: Paragraph title: Amplification of coding regions by RT-PCR ... The reaction, which contained 1× KlentaqLA buffer (pH 9.2), 0.8 mM dNTP, 10% DMSO, 1 mM MgCl2, 0.5 µl KlentaqLA polymerase , was transferred directly from ice to a thermocycler (Bio-Rad, Hercules, CA) that was preheated to 93°C.

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: .. Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C. ..

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: All primers were designed in house to amplify the following pluripotency associated genes: OCT4 , NANOG , and SOX2 while the housekeeping gene β-actin was also amplified to act as a reference. .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK).

Article Title: Prevalence and genetic diversity of Trichomonas vaginalis clinical isolates in a targeted population in Xinxiang City, Henan Province, China
Article Snippet: .. The PCR amplification was conducted using a thermocycler (Bio-Rad, USA) in two steps. .. The first step of the PCR reaction was composed of 5 μl DNA, 12.5 μl master mix (Yi Fei Xue Biotechnology, Nanjing, China), and 2 μl OPs (20 pmol each of OP-F and OP-R) in 25 μl of final volume.

Positive Control:

Article Title: Localization of angiotensin converting enzyme in rabbit cornea and its role in controlling corneal angiogenesis in vivo
Article Snippet: A 50-µl reaction mixture containing 3 µl cDNA, 2 µl forward (200 nM) and 2 µl reverse (200 nM) primer, 3.125 mM of deoxynucleotide triphosphates (dNTPs) and Taq polymearse was run one cycle at 95 °C for 3 min, then 40 cycles of 95 °C 30 s, followed by 55 °C 30 s and 55 °C for 60 s, using a thermocycler (Bio-Rad Laboratories, Hercules, CA). .. For AT2 , the forward primer sequence was 5′-TGA GAA ATA TGC CCA GTG GTC GGT-3′, and the reverse primer sequence was 5′-ATA ATC CAG ATG GGC CTC AAG CCA-3′. β-actin (ACTB ) was used as a positive control gene: forward primer (5′-AGG CCA ACC GCG AGA AGA TGA CC-3′), reverse primer (5′-GAA GTC CAG GGC GAC GTA GCA C-3′). cDNA samples were prepared from two different rabbits.

Polymerase Chain Reaction:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: Paragraph title: Reverse transcript PCR (RT-PCR) and quantitative real time PCR (RT-qPCR) ... For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′.

Article Title: Ulinastatin attenuates diabetes-induced cardiac dysfunction by the inhibition of inflammation and apoptosis
Article Snippet: .. PCR was performed using a thermocycler (IQ5 Real-Time PCR cycler; Bio-Rad Laboratories, Inc., Hercules, CA, USA) with SsoFast EvaGreen Supermix (Bio-Rad Laboratories, Inc.). .. The mRNA levels of the target genes were normalized to the expression level of GADPH.

Article Title: Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain ▿ Strain ▿ †
Article Snippet: The mixture was incubated in a thermocycler (Bio-Rad, Hercules, CA) at 25°C for 10 min, 37°C for 1 h, and then 42°C for 1 h. The reaction was followed by an incubation at 70°C for 10 min to inactivate the superscript. .. QIAquick PCR purification kits were used to clean up the cDNA synthesis product.

Article Title: Introns in the Cytolethal Distending Toxin Gene of Actinobacillus actinomycetemcomitans
Article Snippet: Paragraph title: PCR. ... All PCRs were performed by using a thermocycler (iCycler; Bio-Rad Laboratories) in a final volume of 50 μl.

Article Title: Localization of angiotensin converting enzyme in rabbit cornea and its role in controlling corneal angiogenesis in vivo
Article Snippet: Paragraph title: RNA extraction, cDNA synthesis, and PCR ... A 50-µl reaction mixture containing 3 µl cDNA, 2 µl forward (200 nM) and 2 µl reverse (200 nM) primer, 3.125 mM of deoxynucleotide triphosphates (dNTPs) and Taq polymearse was run one cycle at 95 °C for 3 min, then 40 cycles of 95 °C 30 s, followed by 55 °C 30 s and 55 °C for 60 s, using a thermocycler (Bio-Rad Laboratories, Hercules, CA).

Article Title: Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis ... The RT reaction was performed in thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) at 42°C for 2 min, 37°C for 15 min, and 85°C for 5 sec.

Article Title: TGF-β and TGF-β/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts
Article Snippet: .. The real time RT-PCR was run in a thermocycler (iQ5 Bio-Rad, Hercules, CA, USA) with the SYBR Green PCR premix (TaKaRa, Dalian, China) following the manufacturer’s instructions. ..

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: .. PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California). .. PCR parameters were an initial 95°C for 5 minutes followed by 95°C for 15 seconds and 60°C for 1 minute.

Article Title: Synthesizing and Salvaging NAD+: Lessons Learned from Chlamydomonas reinhardtii
Article Snippet: Five µl cDNA (1/10 of the reaction volume) from above was used in a 50 µl PCR reaction using a 3′ RACE primer and a gene-specific primer (nic1-3) that binds 4 nucleotides downstream of the predicted start codon. .. The reaction, which contained 1× KlentaqLA buffer (pH 9.2), 0.8 mM dNTP, 10% DMSO, 1 mM MgCl2, 0.5 µl KlentaqLA polymerase , was transferred directly from ice to a thermocycler (Bio-Rad, Hercules, CA) that was preheated to 93°C.

Article Title: Tissue-specific evaluation of suitable reference genes for RT-qPCR in the pond snail, Lymnaea stagnalis
Article Snippet: Paragraph title: Reverse transcription PCR ... RT-PCR took place in a Bio-Rad CFX Connect thermocycler (Bio-Rad Laboratories, Hercules, CA, USA).

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: To quantify changes in ProcR expression in knockdown lines, total RNA was isolated from whole larvae (5 larvae per replicate) using Norgen's Total RNA Purification Kit (St. Catharines, ON, Canada), 500 ng of total RNA were reverse transcribed with iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA), and PCR was performed to validate primers (see ). .. Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C.

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK). ..

Article Title: Prevalence and genetic diversity of Trichomonas vaginalis clinical isolates in a targeted population in Xinxiang City, Henan Province, China
Article Snippet: .. The PCR amplification was conducted using a thermocycler (Bio-Rad, USA) in two steps. .. The first step of the PCR reaction was composed of 5 μl DNA, 12.5 μl master mix (Yi Fei Xue Biotechnology, Nanjing, China), and 2 μl OPs (20 pmol each of OP-F and OP-R) in 25 μl of final volume.

Article Title: Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Article Snippet: .. The PCR reactions were conducted in a thermocycler (Bio-rad, USA) with the following program: 94°C for 5 minutes, 35 cycles at 94°C for 45 seconds, 55°C for 45 seconds, 72°C for 45 seconds, and a final extension at 74°C for 10 minutes. .. Ten µg of the PCR product were separated, run on a 1.5% agarose gel, and stained with SYBR safe.

Lambda DNA Preparation:

Article Title: H-NS uses an autoinhibitory conformational switch for environment-controlled gene silencing
Article Snippet: .. Nuclease assay H-NSFL and H-NSΔs2 at 20 μM final concentration were incubated with 1 μg lambda DNA (NEB) at 25 and 37°C in the presence of 5 units of DNAse (EMD Millipore) in a Thermocycler (Biorad) for 30 min. .. Samples were then mixed with 5 μl of 6× loading buffer (NEB) and resolved on a 0.7% agarose gel mixed with 1× Cybersafe (Lab technologies).

Quantitative RT-PCR:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: .. For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′. .. Real Time-PCR was carried out in a I-Cycler IQ Multicolor RT-PCR Detection System using Sso Fast Eva Green Supermix (Biorad).

Article Title: Ulinastatin attenuates diabetes-induced cardiac dysfunction by the inhibition of inflammation and apoptosis
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... PCR was performed using a thermocycler (IQ5 Real-Time PCR cycler; Bio-Rad Laboratories, Inc., Hercules, CA, USA) with SsoFast EvaGreen Supermix (Bio-Rad Laboratories, Inc.).

Article Title: Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis ... The RT reaction was performed in thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) at 42°C for 2 min, 37°C for 15 min, and 85°C for 5 sec.

Article Title: TGF-β and TGF-β/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts
Article Snippet: .. The real time RT-PCR was run in a thermocycler (iQ5 Bio-Rad, Hercules, CA, USA) with the SYBR Green PCR premix (TaKaRa, Dalian, China) following the manufacturer’s instructions. ..

Article Title: Tissue-specific evaluation of suitable reference genes for RT-qPCR in the pond snail, Lymnaea stagnalis
Article Snippet: Reverse transcription PCR Reverse transcription-PCR was performed with iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories, Hercules, CA, USA) which contains a mixture of oligo(dT) primers and random hexamers. .. RT-PCR took place in a Bio-Rad CFX Connect thermocycler (Bio-Rad Laboratories, Hercules, CA, USA).

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: Paragraph title: RT-qPCR. ... Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C.

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK). ..

SYBR Green Assay:

Article Title: TGF-β and TGF-β/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts
Article Snippet: .. The real time RT-PCR was run in a thermocycler (iQ5 Bio-Rad, Hercules, CA, USA) with the SYBR Green PCR premix (TaKaRa, Dalian, China) following the manufacturer’s instructions. ..

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: For real-time qPCR, SYBR Green qPCR Supermix (Invitrogen) was added to the cDNA and the primers. .. Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C.

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK). ..

Nested PCR:

Article Title: Synthesizing and Salvaging NAD+: Lessons Learned from Chlamydomonas reinhardtii
Article Snippet: To remove RNA from the reaction, 2 units of RNase H (Invitrogen) were added at the end of reaction and incubated at 37°C for 20 min. Amplification of the NMNAT coding region requires nested PCR due to highly repetitive sequences found in the gene. .. The reaction, which contained 1× KlentaqLA buffer (pH 9.2), 0.8 mM dNTP, 10% DMSO, 1 mM MgCl2, 0.5 µl KlentaqLA polymerase , was transferred directly from ice to a thermocycler (Bio-Rad, Hercules, CA) that was preheated to 93°C.

Article Title: Prevalence and genetic diversity of Trichomonas vaginalis clinical isolates in a targeted population in Xinxiang City, Henan Province, China
Article Snippet: PCR-RFLP We chose the target of the nested PCR based on the actin gene (GenBank: AF237734). .. The PCR amplification was conducted using a thermocycler (Bio-Rad, USA) in two steps.

Incubation:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: .. For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′. .. Real Time-PCR was carried out in a I-Cycler IQ Multicolor RT-PCR Detection System using Sso Fast Eva Green Supermix (Biorad).

Article Title: Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain ▿ Strain ▿ †
Article Snippet: .. The mixture was incubated in a thermocycler (Bio-Rad, Hercules, CA) at 25°C for 10 min, 37°C for 1 h, and then 42°C for 1 h. The reaction was followed by an incubation at 70°C for 10 min to inactivate the superscript. .. The RNA was then degraded by adding 20 μl of 1 N NaOH and incubation at 65°C for 30 min. After the incubation, 20 μl of 1 N HCl was added to neutralize the solution.

Article Title: Synthesizing and Salvaging NAD+: Lessons Learned from Chlamydomonas reinhardtii
Article Snippet: To remove RNA from the reaction, 2 units of RNase H (Invitrogen) were added at the end of reaction and incubated at 37°C for 20 min. Amplification of the NMNAT coding region requires nested PCR due to highly repetitive sequences found in the gene. .. The reaction, which contained 1× KlentaqLA buffer (pH 9.2), 0.8 mM dNTP, 10% DMSO, 1 mM MgCl2, 0.5 µl KlentaqLA polymerase , was transferred directly from ice to a thermocycler (Bio-Rad, Hercules, CA) that was preheated to 93°C.

Article Title: H-NS uses an autoinhibitory conformational switch for environment-controlled gene silencing
Article Snippet: .. Nuclease assay H-NSFL and H-NSΔs2 at 20 μM final concentration were incubated with 1 μg lambda DNA (NEB) at 25 and 37°C in the presence of 5 units of DNAse (EMD Millipore) in a Thermocycler (Biorad) for 30 min. .. Samples were then mixed with 5 μl of 6× loading buffer (NEB) and resolved on a 0.7% agarose gel mixed with 1× Cybersafe (Lab technologies).

Expressing:

Article Title: Ulinastatin attenuates diabetes-induced cardiac dysfunction by the inhibition of inflammation and apoptosis
Article Snippet: PCR was performed using a thermocycler (IQ5 Real-Time PCR cycler; Bio-Rad Laboratories, Inc., Hercules, CA, USA) with SsoFast EvaGreen Supermix (Bio-Rad Laboratories, Inc.). .. The mRNA levels of the target genes were normalized to the expression level of GADPH.

Article Title: Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway
Article Snippet: The RT reaction was performed in thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) at 42°C for 2 min, 37°C for 15 min, and 85°C for 5 sec. .. Subsequently, The average quantification cycle threshold (Ct) of the triplicate samples was calculated as the quantity of gene pruduct and the relative mRNA expression levels were analyzed by 2−ΔΔCt method ( ) by Rotor-Gene Q series software (Qiagen GmbH, Hilden, Germany) in accordance with the standard PCR conditions (94°C for 10 min, and 40 cycles of 45 sec at 94°C, 30 sec at 59°C, 45 sec at 72°C).

Article Title: TGF-β and TGF-β/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts
Article Snippet: The real time RT-PCR was run in a thermocycler (iQ5 Bio-Rad, Hercules, CA, USA) with the SYBR Green PCR premix (TaKaRa, Dalian, China) following the manufacturer’s instructions. .. To normalize for gene expression, mRNA expression of the housekeeping gene GAPDH was measured.

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: To quantify changes in ProcR expression in knockdown lines, total RNA was isolated from whole larvae (5 larvae per replicate) using Norgen's Total RNA Purification Kit (St. Catharines, ON, Canada), 500 ng of total RNA were reverse transcribed with iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA), and PCR was performed to validate primers (see ). .. Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C.

Dissection:

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: In all studies, the anatomical boundaries for hypothalamus dissection were: the optic chiasm at the anterior border, the mammillary bodies at the posterior border, and laterally at the hypothalamic sulci. .. PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California).

Cell Culture:

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: Real-time PCR (qRT-PCR) Total cellular RNA was isolated from hESCs cultured under both hydrogel and fibronectin conditions using an RNeasy Mini Kit (Qiagen, UK) according to the manufacturer’s recommendations and eluted in 30 μl of RNase free water. .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK).

DNA Sequencing:

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California). .. To verify amplification of the target gene, PCR products were purified (Centricon-100; Millipore, Billerica, Massachusetts) and directly sequenced at the University of Chicago Cancer Research Center DNA Sequencing Facility.

Reverse Transcription Polymerase Chain Reaction:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: Paragraph title: Reverse transcript PCR (RT-PCR) and quantitative real time PCR (RT-qPCR) ... For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′.

Article Title: Synthesizing and Salvaging NAD+: Lessons Learned from Chlamydomonas reinhardtii
Article Snippet: Paragraph title: Amplification of coding regions by RT-PCR ... The reaction, which contained 1× KlentaqLA buffer (pH 9.2), 0.8 mM dNTP, 10% DMSO, 1 mM MgCl2, 0.5 µl KlentaqLA polymerase , was transferred directly from ice to a thermocycler (Bio-Rad, Hercules, CA) that was preheated to 93°C.

Article Title: Tissue-specific evaluation of suitable reference genes for RT-qPCR in the pond snail, Lymnaea stagnalis
Article Snippet: .. RT-PCR took place in a Bio-Rad CFX Connect thermocycler (Bio-Rad Laboratories, Hercules, CA, USA). .. The RT-PCR program ran at 25 °C for 5 min, 46 °C for 20 min and 95 °C for 1 min.

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: For subsequent RT-PCR reactions cDNA synthesised from the reverse transcription reaction was diluted 10 fold with ultraPURE DNase and RNase free ddH2 O (Invitrogen, UK). .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK).

Isolation:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: Reverse transcript PCR (RT-PCR) and quantitative real time PCR (RT-qPCR) Samples were lysed in TRYzol reagent for isolation of total RNA. .. For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′.

Article Title: Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain ▿ Strain ▿ †
Article Snippet: Total RNA was isolated using an RNeasy minikit (Qiagen, Valencia, CA). .. The mixture was incubated in a thermocycler (Bio-Rad, Hercules, CA) at 25°C for 10 min, 37°C for 1 h, and then 42°C for 1 h. The reaction was followed by an incubation at 70°C for 10 min to inactivate the superscript.

Article Title: TGF-β and TGF-β/Smad Signaling in the Interactions between Echinococcus multilocularis and Its Hosts
Article Snippet: Quantitative Real-time RT- PCR Analysis After removing contaminated DNA from the isolated RNA using DNaseI (Fermentas, Vilnius, Lithuania), 1 µg of total RNA was reverse transcribed into cDNA in 20 mL reaction mixtures containing 200 U of Moloney murine leukemia virus reverse transcriptase (MMLV, Promega, Madison, USA); 100 ng per reaction of oligo (dT) primers; and 0.5 mM each of dNTPs, dATP, dCTP, dGTP, and dTTP. .. The real time RT-PCR was run in a thermocycler (iQ5 Bio-Rad, Hercules, CA, USA) with the SYBR Green PCR premix (TaKaRa, Dalian, China) following the manufacturer’s instructions.

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: Paragraph title: Isolation and partial sequencing of dio2 and dio3 mRNA ... PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California).

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: To quantify changes in ProcR expression in knockdown lines, total RNA was isolated from whole larvae (5 larvae per replicate) using Norgen's Total RNA Purification Kit (St. Catharines, ON, Canada), 500 ng of total RNA were reverse transcribed with iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA), and PCR was performed to validate primers (see ). .. Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C.

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: Real-time PCR (qRT-PCR) Total cellular RNA was isolated from hESCs cultured under both hydrogel and fibronectin conditions using an RNeasy Mini Kit (Qiagen, UK) according to the manufacturer’s recommendations and eluted in 30 μl of RNase free water. .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK).

Size-exclusion Chromatography:

Article Title: Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway
Article Snippet: .. The RT reaction was performed in thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) at 42°C for 2 min, 37°C for 15 min, and 85°C for 5 sec. .. Subsequently, The average quantification cycle threshold (Ct) of the triplicate samples was calculated as the quantity of gene pruduct and the relative mRNA expression levels were analyzed by 2−ΔΔCt method ( ) by Rotor-Gene Q series software (Qiagen GmbH, Hilden, Germany) in accordance with the standard PCR conditions (94°C for 10 min, and 40 cycles of 45 sec at 94°C, 30 sec at 59°C, 45 sec at 72°C).

Purification:

Article Title: Ulinastatin attenuates diabetes-induced cardiac dysfunction by the inhibition of inflammation and apoptosis
Article Snippet: Purified RNA (2 µg) was treated with DNase and reverse transcribed (RevertAid M-MulV Reverse Transcriptase; Thermo Fisher Scientific, Inc.) following a program of 42°C for 1 h, 70°C for 5 min. qPCR was performed using the following primers: HMGB1 forward, 5′-ACCTGCCGGGAGGAGCACAA-3′ and reverse, 5′-GCCTCTTGGGGGCATTGG-3′; GAPDH forward, 5′-AGGTCGGTGTGAACGGATTTGGG-3′ and reverse, 5′-TGTAGACCATGTAGTTGAGGTCA-3′. .. PCR was performed using a thermocycler (IQ5 Real-Time PCR cycler; Bio-Rad Laboratories, Inc., Hercules, CA, USA) with SsoFast EvaGreen Supermix (Bio-Rad Laboratories, Inc.).

Article Title: Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain ▿ Strain ▿ †
Article Snippet: The mixture was incubated in a thermocycler (Bio-Rad, Hercules, CA) at 25°C for 10 min, 37°C for 1 h, and then 42°C for 1 h. The reaction was followed by an incubation at 70°C for 10 min to inactivate the superscript. .. QIAquick PCR purification kits were used to clean up the cDNA synthesis product.

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California). .. To verify amplification of the target gene, PCR products were purified (Centricon-100; Millipore, Billerica, Massachusetts) and directly sequenced at the University of Chicago Cancer Research Center DNA Sequencing Facility.

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: To quantify changes in ProcR expression in knockdown lines, total RNA was isolated from whole larvae (5 larvae per replicate) using Norgen's Total RNA Purification Kit (St. Catharines, ON, Canada), 500 ng of total RNA were reverse transcribed with iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA), and PCR was performed to validate primers (see ). .. Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C.

Sequencing:

Article Title: Localization of angiotensin converting enzyme in rabbit cornea and its role in controlling corneal angiogenesis in vivo
Article Snippet: A 50-µl reaction mixture containing 3 µl cDNA, 2 µl forward (200 nM) and 2 µl reverse (200 nM) primer, 3.125 mM of deoxynucleotide triphosphates (dNTPs) and Taq polymearse was run one cycle at 95 °C for 3 min, then 40 cycles of 95 °C 30 s, followed by 55 °C 30 s and 55 °C for 60 s, using a thermocycler (Bio-Rad Laboratories, Hercules, CA). .. For ACE , the forward primer sequence 5′-ACG AGC ACG ACA TCA ACT TCC TCA-3′ and reverse primer sequence 5′-AGT AGT TCA TCA TGG CCG AGG CT-3′ were used.

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: Paragraph title: Isolation and partial sequencing of dio2 and dio3 mRNA ... PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California).

Construct:

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: Total RNA was isolated using RNeasy (QIAGEN, Valencia, California), and a cDNA library was constructed using Molony Murine Leukemia Virus Reverse Transcriptase enzyme (Invitrogen, Carlsbad, California). .. PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California).

Nuclease Assay:

Article Title: H-NS uses an autoinhibitory conformational switch for environment-controlled gene silencing
Article Snippet: .. Nuclease assay H-NSFL and H-NSΔs2 at 20 μM final concentration were incubated with 1 μg lambda DNA (NEB) at 25 and 37°C in the presence of 5 units of DNAse (EMD Millipore) in a Thermocycler (Biorad) for 30 min. .. Samples were then mixed with 5 μl of 6× loading buffer (NEB) and resolved on a 0.7% agarose gel mixed with 1× Cybersafe (Lab technologies).

IA:

Article Title: Synthesizing and Salvaging NAD+: Lessons Learned from Chlamydomonas reinhardtii
Article Snippet: Five µg of total RNA from wild-type cells were used for cDNA synthesis using a 3′ RACE poly (dT)-adaptor primer (Integrated DNA Technologies, Iowa City, IA) in a 50 µl reaction, which contains 1× RT buffer (Invitrogen, San Diego, CA), 10 mM DTT, 0.5 mM dNTP, 0.2 µM primer, 40 U of RNaseOUT (Invitrogen), and 200 U of SuperScript II reverse transcriptase (Invitrogen). .. The reaction, which contained 1× KlentaqLA buffer (pH 9.2), 0.8 mM dNTP, 10% DMSO, 1 mM MgCl2, 0.5 µl KlentaqLA polymerase , was transferred directly from ice to a thermocycler (Bio-Rad, Hercules, CA) that was preheated to 93°C.

Activated Clotting Time Assay:

Article Title: Localization of angiotensin converting enzyme in rabbit cornea and its role in controlling corneal angiogenesis in vivo
Article Snippet: A 50-µl reaction mixture containing 3 µl cDNA, 2 µl forward (200 nM) and 2 µl reverse (200 nM) primer, 3.125 mM of deoxynucleotide triphosphates (dNTPs) and Taq polymearse was run one cycle at 95 °C for 3 min, then 40 cycles of 95 °C 30 s, followed by 55 °C 30 s and 55 °C for 60 s, using a thermocycler (Bio-Rad Laboratories, Hercules, CA). .. For ACE , the forward primer sequence 5′-ACG AGC ACG ACA TCA ACT TCC TCA-3′ and reverse primer sequence 5′-AGT AGT TCA TCA TGG CCG AGG CT-3′ were used.

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: All primers were designed in house to amplify the following pluripotency associated genes: OCT4 , NANOG , and SOX2 while the housekeeping gene β-actin was also amplified to act as a reference. .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK).

Software:

Article Title: Aryl hydrocarbon receptor activation maintained the intestinal epithelial barrier function through Notch1 dependent signaling pathway
Article Snippet: The RT reaction was performed in thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) at 42°C for 2 min, 37°C for 15 min, and 85°C for 5 sec. .. Subsequently, The average quantification cycle threshold (Ct) of the triplicate samples was calculated as the quantity of gene pruduct and the relative mRNA expression levels were analyzed by 2−ΔΔCt method ( ) by Rotor-Gene Q series software (Qiagen GmbH, Hilden, Germany) in accordance with the standard PCR conditions (94°C for 10 min, and 40 cycles of 45 sec at 94°C, 30 sec at 59°C, 45 sec at 72°C).

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: Degenerate primers that targeted dio2 and dio3 were designed based on conserved regions among multiple species with known gene sequences (GenBank) using PrimerExpress software (Applied Biosystems, Foster City, California). .. PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California).

Real-time Polymerase Chain Reaction:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: Paragraph title: Reverse transcript PCR (RT-PCR) and quantitative real time PCR (RT-qPCR) ... For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′.

Article Title: Ulinastatin attenuates diabetes-induced cardiac dysfunction by the inhibition of inflammation and apoptosis
Article Snippet: .. PCR was performed using a thermocycler (IQ5 Real-Time PCR cycler; Bio-Rad Laboratories, Inc., Hercules, CA, USA) with SsoFast EvaGreen Supermix (Bio-Rad Laboratories, Inc.). .. The mRNA levels of the target genes were normalized to the expression level of GADPH.

Article Title: Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain ▿ Strain ▿ †
Article Snippet: Paragraph title: qPCR. ... The mixture was incubated in a thermocycler (Bio-Rad, Hercules, CA) at 25°C for 10 min, 37°C for 1 h, and then 42°C for 1 h. The reaction was followed by an incubation at 70°C for 10 min to inactivate the superscript.

Article Title: Characterizing the physiological and behavioral roles of proctolin in Drosophila melanogaster
Article Snippet: For real-time qPCR, SYBR Green qPCR Supermix (Invitrogen) was added to the cDNA and the primers. .. Each sample was amplified for 40 cycles in a thermocycler (Bio-Rad) as follows: 5 min at 95°C, 15 s at 95°C, 90 s at 56°C, and 30 s at 72°C.

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: Paragraph title: Real-time PCR (qRT-PCR) ... After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK).

RNA Extraction:

Article Title: Localization of angiotensin converting enzyme in rabbit cornea and its role in controlling corneal angiogenesis in vivo
Article Snippet: Paragraph title: RNA extraction, cDNA synthesis, and PCR ... A 50-µl reaction mixture containing 3 µl cDNA, 2 µl forward (200 nM) and 2 µl reverse (200 nM) primer, 3.125 mM of deoxynucleotide triphosphates (dNTPs) and Taq polymearse was run one cycle at 95 °C for 3 min, then 40 cycles of 95 °C 30 s, followed by 55 °C 30 s and 55 °C for 60 s, using a thermocycler (Bio-Rad Laboratories, Hercules, CA).

Article Title: Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Article Snippet: Reverses transcriptase-polymerase chain reaction Total RNA was extracted from the cells using RNA extraction solution (RNX™, Cinnagen, Iran). .. The PCR reactions were conducted in a thermocycler (Bio-rad, USA) with the following program: 94°C for 5 minutes, 35 cycles at 94°C for 45 seconds, 55°C for 45 seconds, 72°C for 45 seconds, and a final extension at 74°C for 10 minutes.

Selection:

Article Title: Prevalence and genetic diversity of Trichomonas vaginalis clinical isolates in a targeted population in Xinxiang City, Henan Province, China
Article Snippet: The selection of outer primers (OPs) and inner primers (IPs) was done according to references [ , ], and the primer sequences are provided in Table . .. The PCR amplification was conducted using a thermocycler (Bio-Rad, USA) in two steps.

Agarose Gel Electrophoresis:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: MJ Mini Thermal Cycler (Bio-Rad) was used for all reactions and amplification products were analyzed on 1.8% agarose gel stained with ethidium bromide. .. For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′.

Article Title: H-NS uses an autoinhibitory conformational switch for environment-controlled gene silencing
Article Snippet: Nuclease assay H-NSFL and H-NSΔs2 at 20 μM final concentration were incubated with 1 μg lambda DNA (NEB) at 25 and 37°C in the presence of 5 units of DNAse (EMD Millipore) in a Thermocycler (Biorad) for 30 min. .. Samples were then mixed with 5 μl of 6× loading buffer (NEB) and resolved on a 0.7% agarose gel mixed with 1× Cybersafe (Lab technologies).

Article Title: Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Article Snippet: The PCR reactions were conducted in a thermocycler (Bio-rad, USA) with the following program: 94°C for 5 minutes, 35 cycles at 94°C for 45 seconds, 55°C for 45 seconds, 72°C for 45 seconds, and a final extension at 74°C for 10 minutes. .. Ten µg of the PCR product were separated, run on a 1.5% agarose gel, and stained with SYBR safe.

Electrophoresis:

Article Title: Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Article Snippet: The quantity and quality of the extracted RNA were checked using nanodrop (Thermo Fisher Scientific, USA) and electrophoresis, respectively. cDNA was synthesized from 5 µg total RNA using a Fermentas kit (Fermentas, Canada) according to the manufacturer’s manuals. .. The PCR reactions were conducted in a thermocycler (Bio-rad, USA) with the following program: 94°C for 5 minutes, 35 cycles at 94°C for 45 seconds, 55°C for 45 seconds, 72°C for 45 seconds, and a final extension at 74°C for 10 minutes.

Spectrophotometry:

Article Title: A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro
Article Snippet: The quantity and purity of RNA was determined by 260/280 nm absorbance using a spectrophotometer. .. After optimisation of the primers to ensure their efficiency and ability to amplify a single PCR product, qRT-PCR was performed on all samples including negative controls using a thermocycler (Bio-Rad, UK) and a stock solution containing SYBR Green single tube real time master mix (Bio-Rad, UK), ultraPURE ddH2 O and a primer solution containing both sense and anti-sense custom designed primers (Invitrogen, UK).

Synthesized:

Article Title: Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Article Snippet: The quantity and quality of the extracted RNA were checked using nanodrop (Thermo Fisher Scientific, USA) and electrophoresis, respectively. cDNA was synthesized from 5 µg total RNA using a Fermentas kit (Fermentas, Canada) according to the manufacturer’s manuals. .. The PCR reactions were conducted in a thermocycler (Bio-rad, USA) with the following program: 94°C for 5 minutes, 35 cycles at 94°C for 45 seconds, 55°C for 45 seconds, 72°C for 45 seconds, and a final extension at 74°C for 10 minutes.

Concentration Assay:

Article Title: H-NS uses an autoinhibitory conformational switch for environment-controlled gene silencing
Article Snippet: .. Nuclease assay H-NSFL and H-NSΔs2 at 20 μM final concentration were incubated with 1 μg lambda DNA (NEB) at 25 and 37°C in the presence of 5 units of DNAse (EMD Millipore) in a Thermocycler (Biorad) for 30 min. .. Samples were then mixed with 5 μl of 6× loading buffer (NEB) and resolved on a 0.7% agarose gel mixed with 1× Cybersafe (Lab technologies).

cDNA Library Assay:

Article Title: Rapid Induction of Hypothalamic Iodothyronine Deiodinase Expression by Photoperiod and Melatonin in Juvenile Siberian Hamsters (Phodopus sungorus)
Article Snippet: Total RNA was isolated using RNeasy (QIAGEN, Valencia, California), and a cDNA library was constructed using Molony Murine Leukemia Virus Reverse Transcriptase enzyme (Invitrogen, Carlsbad, California). .. PCR was conducted on 1 μL of pooled hamster hypothalamic cDNA with Taq DNA Polymerase enzyme (Invitrogen) according to the manufacturer's protocol in a thermocycler for 40 cycles (Bio-Rad, Hercules, California).

CTG Assay:

Article Title: Localization of angiotensin converting enzyme in rabbit cornea and its role in controlling corneal angiogenesis in vivo
Article Snippet: A 50-µl reaction mixture containing 3 µl cDNA, 2 µl forward (200 nM) and 2 µl reverse (200 nM) primer, 3.125 mM of deoxynucleotide triphosphates (dNTPs) and Taq polymearse was run one cycle at 95 °C for 3 min, then 40 cycles of 95 °C 30 s, followed by 55 °C 30 s and 55 °C for 60 s, using a thermocycler (Bio-Rad Laboratories, Hercules, CA). .. For AT1 , the forward primer sequence was 5′-AGG ATG ACT GTC CCA AAG CTG GAA-3′, and the reverse primer sequence was 5′-ACG TTT CGG TGG ATG ATA GCT GGT-3′.

Staining:

Article Title: CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
Article Snippet: MJ Mini Thermal Cycler (Bio-Rad) was used for all reactions and amplification products were analyzed on 1.8% agarose gel stained with ethidium bromide. .. For RT-qPCR Reverse transcription reaction was performed in a thermocycler using IScript TM RT Supermix (Biorad) under the following conditions: incubation, 25°C, 5′; reverse transcription, 42°C, 45′; inactivation, 85°C, 5′.

Article Title: Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Article Snippet: The PCR reactions were conducted in a thermocycler (Bio-rad, USA) with the following program: 94°C for 5 minutes, 35 cycles at 94°C for 45 seconds, 55°C for 45 seconds, 72°C for 45 seconds, and a final extension at 74°C for 10 minutes. .. Ten µg of the PCR product were separated, run on a 1.5% agarose gel, and stained with SYBR safe.

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  • 78
    Bio-Rad programmable pcr thermocycler
    Magnesium carbonate microhemispheres synthesized with a programmable <t>PCR</t> <t>thermocycler.</t> The reactant was a mixture of 240 2 and 500 mM NaHCO 3 (1∶1, V/V). (A) Similar temperature oscillation patterns having different periods. (B–D) Transmitted light microscopic images of microhemispheres produced by the temperature oscillation patterns with 60, 30 and 15-min period, respectively. The corresponding layer thicknesses were 12.0±0.2, 6.0±0.1 and 2.6±0.0 µm.
    Programmable Pcr Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/programmable pcr thermocycler/product/Bio-Rad
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    programmable pcr thermocycler - by Bioz Stars, 2020-02
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    99
    Bio-Rad thermocycler
    On-chip detection of whole bacterial cells. ( a ) Fluorescence imaging of a representative integrated μSPE-HDA chip showing positive HDA reactions done in triplicate and a negative control water reaction. Reactions were positive for HDA of E. coli dxs from broth culture containing 10 9 CFU loaded into the integrated chip. DNA was extracted by the μSPE column, mixed with HDA reaction mix in the HDA chamber, and flowed into the separate reaction wells. ( b ) The HDA products from three integrated chips (imaged in a ) were analyzed for yield concentrations and compared to products from reactions done in an RT <t>thermocycler</t> from integrated chip μSPE DNA extractions. ( c ) Dilutions of E. coli were prepared and 10 5 –10 1 CFU were run through the μSPE channels and then HDA was performed on-chip and in an RT thermocycler. The product yields were compared using an Agilent 2100 Bioanalyzer and were shown to be positive for the correct size 70 bp product
    Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler/product/Bio-Rad
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    thermocycler - by Bioz Stars, 2020-02
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    99
    Bio-Rad ptc 200 thermocycler
    Temperature characterizations of the modified thermal block used in the <t>PTC-200</t> <t>thermocycler</t> (A–B) and a Comsol-model to determine the on-device error (C). The deviation in the actual and reported temperatures are provided for the uncalibrated thermal block (A) and for the block after calibration (B). The Comsol-modeled deviation (C) provides the difference between the actual block temperatures and the theoretical reaction temperatures of the well volumes in a SlipChip microfluidic device. Error values are the difference between the thermocycler-reported thermal block temperature and either the actual thermal block temperatures (measured by a type K thermocouple) or the modeled device temperature; a positive number indicates the thermal block temperature is higher than the device temperature.
    Ptc 200 Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
    ptc 200 thermocycler - by Bioz Stars, 2020-02
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    99
    Bio-Rad real time thermocycler
    Comparison of MS2 RT-LAMP with wet and dried reagents. (A) Ten-fold serial dilutions of MS2 phage particles was made in water and used directly as template in reaction mixture. Amplification was performed on a real time <t>thermocycler</t> in duplicate and time to results was recorded. (B) LAMP reaction products were separated on 2% agarose gel.
    Real Time Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    real time thermocycler - by Bioz Stars, 2020-02
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    Magnesium carbonate microhemispheres synthesized with a programmable PCR thermocycler. The reactant was a mixture of 240 2 and 500 mM NaHCO 3 (1∶1, V/V). (A) Similar temperature oscillation patterns having different periods. (B–D) Transmitted light microscopic images of microhemispheres produced by the temperature oscillation patterns with 60, 30 and 15-min period, respectively. The corresponding layer thicknesses were 12.0±0.2, 6.0±0.1 and 2.6±0.0 µm.

    Journal: PLoS ONE

    Article Title: Temperature Oscillation Modulated Self-Assembly of Periodic Concentric Layered Magnesium Carbonate Microparticles

    doi: 10.1371/journal.pone.0088648

    Figure Lengend Snippet: Magnesium carbonate microhemispheres synthesized with a programmable PCR thermocycler. The reactant was a mixture of 240 2 and 500 mM NaHCO 3 (1∶1, V/V). (A) Similar temperature oscillation patterns having different periods. (B–D) Transmitted light microscopic images of microhemispheres produced by the temperature oscillation patterns with 60, 30 and 15-min period, respectively. The corresponding layer thicknesses were 12.0±0.2, 6.0±0.1 and 2.6±0.0 µm.

    Article Snippet: Many samples were also incubated in a programmable PCR thermocycler (DNA Engine Tetrad 2 thermal cycler, Bio-Rad), while the lid temperature was set to 100°C to prevent vapor condensation.

    Techniques: Synthesized, Polymerase Chain Reaction, Produced

    On-chip detection of whole bacterial cells. ( a ) Fluorescence imaging of a representative integrated μSPE-HDA chip showing positive HDA reactions done in triplicate and a negative control water reaction. Reactions were positive for HDA of E. coli dxs from broth culture containing 10 9 CFU loaded into the integrated chip. DNA was extracted by the μSPE column, mixed with HDA reaction mix in the HDA chamber, and flowed into the separate reaction wells. ( b ) The HDA products from three integrated chips (imaged in a ) were analyzed for yield concentrations and compared to products from reactions done in an RT thermocycler from integrated chip μSPE DNA extractions. ( c ) Dilutions of E. coli were prepared and 10 5 –10 1 CFU were run through the μSPE channels and then HDA was performed on-chip and in an RT thermocycler. The product yields were compared using an Agilent 2100 Bioanalyzer and were shown to be positive for the correct size 70 bp product

    Journal: Biomedical microdevices

    Article Title: An integrated disposable device for DNA extraction and helicase dependent amplification

    doi: 10.1007/s10544-009-9391-8

    Figure Lengend Snippet: On-chip detection of whole bacterial cells. ( a ) Fluorescence imaging of a representative integrated μSPE-HDA chip showing positive HDA reactions done in triplicate and a negative control water reaction. Reactions were positive for HDA of E. coli dxs from broth culture containing 10 9 CFU loaded into the integrated chip. DNA was extracted by the μSPE column, mixed with HDA reaction mix in the HDA chamber, and flowed into the separate reaction wells. ( b ) The HDA products from three integrated chips (imaged in a ) were analyzed for yield concentrations and compared to products from reactions done in an RT thermocycler from integrated chip μSPE DNA extractions. ( c ) Dilutions of E. coli were prepared and 10 5 –10 1 CFU were run through the μSPE channels and then HDA was performed on-chip and in an RT thermocycler. The product yields were compared using an Agilent 2100 Bioanalyzer and were shown to be positive for the correct size 70 bp product

    Article Snippet: The reaction was run either in a thermocycler (AB 7300 Real-time (RT) system, Applied Biosystems, Foster City, CA) or on-chip using a dry heat block as a heat source at 65°C for 1 h. To run HDA reactions in the RT machine (which is not generally set up to run isothermal reactions), cycles were entered as 66°C for 5 s and 65°C for 1 min 55 s. Thus one cycle represented 2 min. EvaGreen and ROX dyes (Biotium Inc., Hayward, CA, USA) were used for detection in the thermocycler and to visualize amplified products on-chip by UV illumination in a Versadoc Gel Imaging System (Bio-Rad, Hercules, CA).

    Techniques: Chromatin Immunoprecipitation, Fluorescence, Imaging, Helicase-dependent Amplification, Negative Control

    Comparison of quantitative HDA and PCR methods from μSPE channel extracted DNA. E. coli DNA from 10 5 to 10 1 CFU total was extracted by μSPE channels in triplicate. ( a ) Real time quantitative HDA and PCR results are shown for amplification of the dxs gene. Reactions were performed in an Applied Biosystems 7300 PCR thermocycler. PCR cycling conditions: 50°C, 2 min; 95°C, 10 min; 40 cycles of 95°C, 15 s; 55°C, 1 min. HDA cycling conditions: 40 cycles of 66°C, 5 s; 65°C 1 min 55 s. ( b ) The average Ct and time to detection of the HDA versus PCR products was determined

    Journal: Biomedical microdevices

    Article Title: An integrated disposable device for DNA extraction and helicase dependent amplification

    doi: 10.1007/s10544-009-9391-8

    Figure Lengend Snippet: Comparison of quantitative HDA and PCR methods from μSPE channel extracted DNA. E. coli DNA from 10 5 to 10 1 CFU total was extracted by μSPE channels in triplicate. ( a ) Real time quantitative HDA and PCR results are shown for amplification of the dxs gene. Reactions were performed in an Applied Biosystems 7300 PCR thermocycler. PCR cycling conditions: 50°C, 2 min; 95°C, 10 min; 40 cycles of 95°C, 15 s; 55°C, 1 min. HDA cycling conditions: 40 cycles of 66°C, 5 s; 65°C 1 min 55 s. ( b ) The average Ct and time to detection of the HDA versus PCR products was determined

    Article Snippet: The reaction was run either in a thermocycler (AB 7300 Real-time (RT) system, Applied Biosystems, Foster City, CA) or on-chip using a dry heat block as a heat source at 65°C for 1 h. To run HDA reactions in the RT machine (which is not generally set up to run isothermal reactions), cycles were entered as 66°C for 5 s and 65°C for 1 min 55 s. Thus one cycle represented 2 min. EvaGreen and ROX dyes (Biotium Inc., Hayward, CA, USA) were used for detection in the thermocycler and to visualize amplified products on-chip by UV illumination in a Versadoc Gel Imaging System (Bio-Rad, Hercules, CA).

    Techniques: Helicase-dependent Amplification, Polymerase Chain Reaction, Amplification

    Temperature characterizations of the modified thermal block used in the PTC-200 thermocycler (A–B) and a Comsol-model to determine the on-device error (C). The deviation in the actual and reported temperatures are provided for the uncalibrated thermal block (A) and for the block after calibration (B). The Comsol-modeled deviation (C) provides the difference between the actual block temperatures and the theoretical reaction temperatures of the well volumes in a SlipChip microfluidic device. Error values are the difference between the thermocycler-reported thermal block temperature and either the actual thermal block temperatures (measured by a type K thermocouple) or the modeled device temperature; a positive number indicates the thermal block temperature is higher than the device temperature.

    Journal: PLoS ONE

    Article Title: Instrument for Real-Time Digital Nucleic Acid Amplification on Custom Microfluidic Devices

    doi: 10.1371/journal.pone.0163060

    Figure Lengend Snippet: Temperature characterizations of the modified thermal block used in the PTC-200 thermocycler (A–B) and a Comsol-model to determine the on-device error (C). The deviation in the actual and reported temperatures are provided for the uncalibrated thermal block (A) and for the block after calibration (B). The Comsol-modeled deviation (C) provides the difference between the actual block temperatures and the theoretical reaction temperatures of the well volumes in a SlipChip microfluidic device. Error values are the difference between the thermocycler-reported thermal block temperature and either the actual thermal block temperatures (measured by a type K thermocouple) or the modeled device temperature; a positive number indicates the thermal block temperature is higher than the device temperature.

    Article Snippet: Temperature control Temperature control is provided by a PTC-200 thermocycler (Bio-Rad, Hercules, CA, USA).

    Techniques: Modification, Blocking Assay

    Comparison of MS2 RT-LAMP with wet and dried reagents. (A) Ten-fold serial dilutions of MS2 phage particles was made in water and used directly as template in reaction mixture. Amplification was performed on a real time thermocycler in duplicate and time to results was recorded. (B) LAMP reaction products were separated on 2% agarose gel.

    Journal: Frontiers in Microbiology

    Article Title: A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP)

    doi: 10.3389/fmicb.2014.00395

    Figure Lengend Snippet: Comparison of MS2 RT-LAMP with wet and dried reagents. (A) Ten-fold serial dilutions of MS2 phage particles was made in water and used directly as template in reaction mixture. Amplification was performed on a real time thermocycler in duplicate and time to results was recorded. (B) LAMP reaction products were separated on 2% agarose gel.

    Article Snippet: Final concentration of the reaction mixes were: 1X OmniAmp Master Mix, 2 mM Fiona Green dye (Marker Gene, OR), and 1X LAMP primer mix (IDT, IA; stock solution: 20X); 5 μl of target (DNA or RNA), brought to volume (25 μl) with DNase-RNase free water and incubated in a real time thermocycler (iQ5, Bio-Rad, CA) at constant temperature for indicated times and monitored by detection of Fiona Green fluorescence, measured and quantified by the instrument software at 30 s intervals.

    Techniques: Amplification, Agarose Gel Electrophoresis

    Detection of DNA targets: Staphylococcus aureus ( Staph. aureus ), Bacillus atrophaeus (BAT), and Porcine circovirus (PCV-2) using OmniAmp LAMP assays. (A) For each pathogen, 10-fold serial dilutions of extracted DNA were prepared in water and used as template in LAMP reaction. Amplification was performed on a real time thermocycler in triplicate with average TTRs shown for each dilution. (No amplification is indicated by “ ** ”) . (B) PCV-2 LAMP products were separated on 2% agarose gel.

    Journal: Frontiers in Microbiology

    Article Title: A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP)

    doi: 10.3389/fmicb.2014.00395

    Figure Lengend Snippet: Detection of DNA targets: Staphylococcus aureus ( Staph. aureus ), Bacillus atrophaeus (BAT), and Porcine circovirus (PCV-2) using OmniAmp LAMP assays. (A) For each pathogen, 10-fold serial dilutions of extracted DNA were prepared in water and used as template in LAMP reaction. Amplification was performed on a real time thermocycler in triplicate with average TTRs shown for each dilution. (No amplification is indicated by “ ** ”) . (B) PCV-2 LAMP products were separated on 2% agarose gel.

    Article Snippet: Final concentration of the reaction mixes were: 1X OmniAmp Master Mix, 2 mM Fiona Green dye (Marker Gene, OR), and 1X LAMP primer mix (IDT, IA; stock solution: 20X); 5 μl of target (DNA or RNA), brought to volume (25 μl) with DNase-RNase free water and incubated in a real time thermocycler (iQ5, Bio-Rad, CA) at constant temperature for indicated times and monitored by detection of Fiona Green fluorescence, measured and quantified by the instrument software at 30 s intervals.

    Techniques: Amplification, Agarose Gel Electrophoresis

    Sensitivity of Porcine circovirus-2 (PCV-2) LAMP . 10-fold serial dilutions of extracted DNA were prepared in water and used as template in LAMP reaction. Amplification was performed on a real time thermocycler in triplicate with average TTRs shown for each dilution.

    Journal: Frontiers in Microbiology

    Article Title: A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP)

    doi: 10.3389/fmicb.2014.00395

    Figure Lengend Snippet: Sensitivity of Porcine circovirus-2 (PCV-2) LAMP . 10-fold serial dilutions of extracted DNA were prepared in water and used as template in LAMP reaction. Amplification was performed on a real time thermocycler in triplicate with average TTRs shown for each dilution.

    Article Snippet: Final concentration of the reaction mixes were: 1X OmniAmp Master Mix, 2 mM Fiona Green dye (Marker Gene, OR), and 1X LAMP primer mix (IDT, IA; stock solution: 20X); 5 μl of target (DNA or RNA), brought to volume (25 μl) with DNase-RNase free water and incubated in a real time thermocycler (iQ5, Bio-Rad, CA) at constant temperature for indicated times and monitored by detection of Fiona Green fluorescence, measured and quantified by the instrument software at 30 s intervals.

    Techniques: Amplification