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Eppendorf AG thermocycler 2
Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on <t>thermocycler</t> 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).
Thermocycler 2, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thermocycler 2/product/Eppendorf AG
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
thermocycler 2 - by Bioz Stars, 2020-05
85/100 stars

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1) Product Images from "Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries"

Article Title: Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

Journal: Genome Biology

doi: 10.1186/gb-2011-12-2-r18

Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on thermocycler 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).
Figure Legend Snippet: Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on thermocycler 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).

Techniques Used: Polymerase Chain Reaction

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  • 85
    Eppendorf AG thermocycler 2
    Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on <t>thermocycler</t> 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).
    Thermocycler 2, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermocycler 2/product/Eppendorf AG
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermocycler 2 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    90
    Eppendorf AG mastercycler ep realplex 2 thermocycler
    Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on <t>thermocycler</t> 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).
    Mastercycler Ep Realplex 2 Thermocycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mastercycler ep realplex 2 thermocycler/product/Eppendorf AG
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mastercycler ep realplex 2 thermocycler - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    Eppendorf AG mastercycler realplex 2
    Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on <t>thermocycler</t> 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).
    Mastercycler Realplex 2, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mastercycler realplex 2/product/Eppendorf AG
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    mastercycler realplex 2 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    88
    Eppendorf AG thermocycler realplex real time pcr system
    Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on <t>thermocycler</t> 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).
    Thermocycler Realplex Real Time Pcr System, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thermocycler realplex real time pcr system - by Bioz Stars, 2020-05
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    Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on thermocycler 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).

    Journal: Genome Biology

    Article Title: Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

    doi: 10.1186/gb-2011-12-2-r18

    Figure Lengend Snippet: Effect of temperature ramp rates . The standard PCR protocol with Phusion HF DNA polymerase and short initial (30 s) and in-cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings. Heating and cooling rates were 6°C/s and 4.5°C/s on thermocycler 1 (bright red line), 4°C/s and 3°C/s on thermocycler 2 (purple line) and 2.2°C/s and 2.2°C/s on thermocycler 3 (dark red line).

    Article Snippet: Thermocycler 2 was an Eppendorf Mastercycler epgradient (no 'S').

    Techniques: Polymerase Chain Reaction