therascreen egfr rgq pcr kit  (Qiagen)


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    Name:
    therascreen EGFR RGQ PCR Kit
    Description:
    For the confident detection of mutations in the EGFR oncogene Kit contents For 24 reactions 1 Control Assay 7 Mutation Assays Positive Control Water Taq DNA Polymerase Benefits FDA approved for in vitro diagnostic use with GILOTRIF or IRESSA Ready to use system with simple workflow Specific and sensitive due to ARMS and Scorpions PCR technolog
    Catalog Number:
    870121
    Price:
    6810
    Category:
    therascreen EGFR RGQ PCR Kit
    Buy from Supplier


    Structured Review

    Qiagen therascreen egfr rgq pcr kit
    therascreen EGFR RGQ PCR Kit
    For the confident detection of mutations in the EGFR oncogene Kit contents For 24 reactions 1 Control Assay 7 Mutation Assays Positive Control Water Taq DNA Polymerase Benefits FDA approved for in vitro diagnostic use with GILOTRIF or IRESSA Ready to use system with simple workflow Specific and sensitive due to ARMS and Scorpions PCR technolog
    https://www.bioz.com/result/therascreen egfr rgq pcr kit/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    therascreen egfr rgq pcr kit - by Bioz Stars, 2021-05
    86/100 stars

    Images

    1) Product Images from "The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations"

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations

    Journal: Medicine

    doi: 10.1097/MD.0000000000003991

    The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.
    Figure Legend Snippet: The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    2) Product Images from "Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma"

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S132187

    The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.
    Figure Legend Snippet: The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.

    Techniques Used: Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: The concentrations of the DNA samples were measured by NanoDrop Lite spectrophotometer system (Thermo Fisher Scientific, Waltham, MA, USA). .. The therascreen EGFR RGQ PCR Kit (Qiagen Ltd.) was custom-made for detecting 29 somatic mutations in exons 18-21 of the EGFR gene using a RT-PCR technology. .. The amplification reaction was done on the Rotor-Gene Q instrument (Qiagen Ltd.).

    Article Title: Preventing and treating brain metastases with three first-line EGFR-tyrosine kinase inhibitors in patients with EGFR mutation-positive advanced non-small cell lung cancer
    Article Snippet: Deoxyribonucleic acid (DNA) was extracted using the QIAcube automated extractor (Qiagen Hilden, Germany) with the QIAamp DNA FFPE tissue kit (Qiagen) and eluted in ATE (QIAmp Tissue Elution) buffer (Qiagen) according to the manufacturer’s instructions. .. The presence of EGFR mutations was determined using the EGFR polymerase chain reaction (PCR) Kit (EGFR RUO Kit) and therascreen® EGFR RGQ PCR Kit (EGFR IVD Kit, Qiagen, Manchester, UK). .. These kits combine Scorpion’s and the amplification-refractory mutation system (ARMS) technologies to detect the mutations using real-time quantitative PCR.

    Article Title: Comparison of plasma ctDNA and tissue/cytology-based techniques for the detection of EGFR mutation status in advanced NSCLC: Spanish data subset from ASSESS
    Article Snippet: Information related to the procedures for EGFR mutation testing is shown in Table . .. The most commonly used EGFR mutation testing method for the tumor tissue samples was the QIAGEN therascreen® EGFR RGQ PCR kit (QIAGEN, Manchester, UK; n = 76; 52.1%) followed by the Roche cobas® EGFR Mutation Test (Roche Molecular Diagnostics, Pleasanton, CA; n = 41; 28.1%). .. The EGFR mutation testing method used for all plasma samples was Fragment Length Analysis + PNA LNA Clamp.

    Article Title: Clinical activity of ASP8273 in Asian patients with non‐small‐cell lung cancer with EGFR activating and T790M mutations, et al. Clinical activity of ASP8273 in Asian patients with non‐small‐cell lung cancer with EGFR activating and T790M mutations
    Article Snippet: Phase II blood sampling was carried out cycle 1 day 1 (predose) and 0.5, 1, 2, 4, 6, 8, and 24 hours postdose; also before dosing on cycle 1 day 2, and days 8, 15, and 21. .. An EGFR‐mutation test using histological or cytological samples, optional in phase I but required in phase II, were analyzed at a central EGFR gene testing laboratory using the therascreen EGFR RGQ PCR kit (Qiagen, Hilden, Germany). ..

    Article Title: Epidermal growth factor receptor tyrosine kinase (EGFR-TK) mutation testing in adults with locally advanced or metastatic non-small cell lung cancer: a systematic review and cost-effectiveness analysis.
    Article Snippet: There were no clear differences in the treatment effects reported by different studies, regardless of which EGFR mutation test was used to select patients. .. Cost-effectiveness analysis using 'Evidence on comparative effectiveness available' and 'Linked evidence' approaches: Therascreen(®) EGFR polymerase chain reaction (PCR) Kit (Qiagen, Venlo, the Netherlands) was both less effective and less costly than direct sequencing of all exon 19-21 mutations at an incremental cost-effectiveness ratio of £32,167 (comparative) and £32,190 (linked) per QALY lost. .. 'Assumption of equal prognostic value' approach: the lowest total strategy cost was [commercial-in-confidence (CiC) information has been removed] [Sanger sequencing or Roche cobas EGFR Mutation Testing Kit(®) (Roche Molecular Systems, Inc., Branchburg, NJ, USA)] compared with (CiC information has been removed) for the most expensive strategy (fragment length analysis combined with pyrosequencing).

    Article Title: Clinical management and outcome of patients with advanced NSCLC carrying EGFR mutations in Spain
    Article Snippet: EGFR mutation testing was mainly performed using quantitative polymerase chain reaction (qPCR)-based tests (90.0%). .. The Therascreen® EGFR RGQ PCR kit (QUIAGEN) was the most frequently used method for mutation testing (47.0%). .. EGFR mutation analysis was performed on biopsy tumour samples in 123 (68.7%) patients and on cytology specimens in 55 (30.7%) patients.

    Article Title: Uncommon EGFR mutations in cytological specimens of 1,874 newly diagnosed Indonesian lung cancer patients
    Article Snippet: Primer pairs for EGFR exon 18 were designed with primer-BLAST software and for EGFR exon 20 was described previously. .. Some samples carrying mutation in exon 20 T790M were retested using Therascreen EGFR RGQ PCR kit (Qiagen, Manchester, UK) and/or AmoyDx EGFR 29 mutations detection kit (AmoyDx, Xiamen, China), according to the manufacturer’s instructions. .. PCR amplification products were purified using the ExoSAP-IT PCR Product Cleanup (Affymetrix/USB, Santa Clara, CA, USA) according to the manufacturer’s protocol.

    Article Title: Role of circulating tumor DNA in the management of early‐stage lung cancer
    Article Snippet: As representative of PCR‐based methods, Qiagen TheraScreen and Cobas EGFR mutation detection kits for plasma have been approved as in vitro diagnostic products (IVD) in the European Union and the US/Japan, respectively. .. The Qiagen TheraScreen EGFR RGQ Plasma PCR Kit (Qiagen, Hilden, Germany) is based on a combination of ARMS PCR and Scorpion technology, designed for 29 mutations detection in the EGFR gene and serves as the companion diagnostic blood test for NSCLC treatment. .. The Cobas EGFR mutation Test v2 (Roche, Basel, Switzerland) could identify 42 mutations in the EGFR gene, and can not only be used as an aid in selecting eligible NSCLC patients for EGFR‐TKI therapy but also as a companion diagnostic to help identify NSCLC patients harboring a T790M mutation, the most common resistance mechanism of EGFR‐TKI therapy.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: The concentrations of the DNA samples were measured by NanoDrop Lite spectrophotometer system (Thermo Fisher Scientific, Waltham, MA, USA). .. The therascreen EGFR RGQ PCR Kit (Qiagen Ltd.) was custom-made for detecting 29 somatic mutations in exons 18-21 of the EGFR gene using a RT-PCR technology. .. The amplification reaction was done on the Rotor-Gene Q instrument (Qiagen Ltd.).

    Mutagenesis:

    Article Title: Comparison of plasma ctDNA and tissue/cytology-based techniques for the detection of EGFR mutation status in advanced NSCLC: Spanish data subset from ASSESS
    Article Snippet: Information related to the procedures for EGFR mutation testing is shown in Table . .. The most commonly used EGFR mutation testing method for the tumor tissue samples was the QIAGEN therascreen® EGFR RGQ PCR kit (QIAGEN, Manchester, UK; n = 76; 52.1%) followed by the Roche cobas® EGFR Mutation Test (Roche Molecular Diagnostics, Pleasanton, CA; n = 41; 28.1%). .. The EGFR mutation testing method used for all plasma samples was Fragment Length Analysis + PNA LNA Clamp.

    Article Title: Clinical management and outcome of patients with advanced NSCLC carrying EGFR mutations in Spain
    Article Snippet: EGFR mutation testing was mainly performed using quantitative polymerase chain reaction (qPCR)-based tests (90.0%). .. The Therascreen® EGFR RGQ PCR kit (QUIAGEN) was the most frequently used method for mutation testing (47.0%). .. EGFR mutation analysis was performed on biopsy tumour samples in 123 (68.7%) patients and on cytology specimens in 55 (30.7%) patients.

    Article Title: Uncommon EGFR mutations in cytological specimens of 1,874 newly diagnosed Indonesian lung cancer patients
    Article Snippet: Primer pairs for EGFR exon 18 were designed with primer-BLAST software and for EGFR exon 20 was described previously. .. Some samples carrying mutation in exon 20 T790M were retested using Therascreen EGFR RGQ PCR kit (Qiagen, Manchester, UK) and/or AmoyDx EGFR 29 mutations detection kit (AmoyDx, Xiamen, China), according to the manufacturer’s instructions. .. PCR amplification products were purified using the ExoSAP-IT PCR Product Cleanup (Affymetrix/USB, Santa Clara, CA, USA) according to the manufacturer’s protocol.

    Sequencing:

    Article Title: Epidermal growth factor receptor tyrosine kinase (EGFR-TK) mutation testing in adults with locally advanced or metastatic non-small cell lung cancer: a systematic review and cost-effectiveness analysis.
    Article Snippet: There were no clear differences in the treatment effects reported by different studies, regardless of which EGFR mutation test was used to select patients. .. Cost-effectiveness analysis using 'Evidence on comparative effectiveness available' and 'Linked evidence' approaches: Therascreen(®) EGFR polymerase chain reaction (PCR) Kit (Qiagen, Venlo, the Netherlands) was both less effective and less costly than direct sequencing of all exon 19-21 mutations at an incremental cost-effectiveness ratio of £32,167 (comparative) and £32,190 (linked) per QALY lost. .. 'Assumption of equal prognostic value' approach: the lowest total strategy cost was [commercial-in-confidence (CiC) information has been removed] [Sanger sequencing or Roche cobas EGFR Mutation Testing Kit(®) (Roche Molecular Systems, Inc., Branchburg, NJ, USA)] compared with (CiC information has been removed) for the most expensive strategy (fragment length analysis combined with pyrosequencing).

    Diagnostic Assay:

    Article Title: Role of circulating tumor DNA in the management of early‐stage lung cancer
    Article Snippet: As representative of PCR‐based methods, Qiagen TheraScreen and Cobas EGFR mutation detection kits for plasma have been approved as in vitro diagnostic products (IVD) in the European Union and the US/Japan, respectively. .. The Qiagen TheraScreen EGFR RGQ Plasma PCR Kit (Qiagen, Hilden, Germany) is based on a combination of ARMS PCR and Scorpion technology, designed for 29 mutations detection in the EGFR gene and serves as the companion diagnostic blood test for NSCLC treatment. .. The Cobas EGFR mutation Test v2 (Roche, Basel, Switzerland) could identify 42 mutations in the EGFR gene, and can not only be used as an aid in selecting eligible NSCLC patients for EGFR‐TKI therapy but also as a companion diagnostic to help identify NSCLC patients harboring a T790M mutation, the most common resistance mechanism of EGFR‐TKI therapy.

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  • 86
    Qiagen egfr rgq pcr kit
    Real-time <t>PCR</t> analysis of <t>EGFR</t> and KRAS mutations in pulmonary adenocarcinoma. (a) EGFR control assay. (b) EGFR exon 19 deletion. (c) EGFR exon 19 deletion and exon 21 L858R dual mutation. (d) KRAS control assay. (e) KRAS exon 2 G12A point mutation. (f) KRAS exon 2 G12V and G12C dual mutation.
    Egfr Rgq Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr rgq pcr kit/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egfr rgq pcr kit - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Qiagen therascreen egfr rgq pcr kit
    The association of the percentage of <t>EGFR</t> mutant DNA and delta Ct determined by the <t>Therascreen</t> EGFR <t>RGQ</t> <t>PCR</t> kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.
    Therascreen Egfr Rgq Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/therascreen egfr rgq pcr kit/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    therascreen egfr rgq pcr kit - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Real-time PCR analysis of EGFR and KRAS mutations in pulmonary adenocarcinoma. (a) EGFR control assay. (b) EGFR exon 19 deletion. (c) EGFR exon 19 deletion and exon 21 L858R dual mutation. (d) KRAS control assay. (e) KRAS exon 2 G12A point mutation. (f) KRAS exon 2 G12V and G12C dual mutation.

    Journal: Current problems in cancer

    Article Title: Epidermal growth factor receptor (EGFR), KRAS, and BRAF mutations in lung adenocarcinomas: A study from India

    doi: 10.1016/j.currproblcancer.2018.12.003

    Figure Lengend Snippet: Real-time PCR analysis of EGFR and KRAS mutations in pulmonary adenocarcinoma. (a) EGFR control assay. (b) EGFR exon 19 deletion. (c) EGFR exon 19 deletion and exon 21 L858R dual mutation. (d) KRAS control assay. (e) KRAS exon 2 G12A point mutation. (f) KRAS exon 2 G12V and G12C dual mutation.

    Article Snippet: The EGFR RGQ PCR Kit (cat no. 870111, Therascreen, Qiagen Ltd, Manchester, UK) was used for detecting the presence of 29 EGFR mutations spanning exons 18–21.

    Techniques: Real-time Polymerase Chain Reaction, Control Assay, Mutagenesis

    The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Journal: Medicine

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations

    doi: 10.1097/MD.0000000000003991

    Figure Lengend Snippet: The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Article Snippet: 3.2 The percentage of mutant EGFR DNA correlates with dCt To evaluate the association of dCt and the amount of mutant EGFR DNA, exon 19 deletions, and exon 21 L858R EGFR mutations were determined by the Therascreen EGFR RGQ PCR kit under different percentages of the mutant EGFR DNA.

    Techniques: Mutagenesis, Polymerase Chain Reaction