therascreen egfr rgq pcr kit  (Qiagen)

 
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    Name:
    therascreen EGFR RGQ PCR Kit
    Description:
    For the confident detection of mutations in the EGFR oncogene Kit contents For 24 reactions 1 Control Assay 7 Mutation Assays Positive Control Water Taq DNA Polymerase Benefits FDA approved for in vitro diagnostic use with GILOTRIF or IRESSA Ready to use system with simple workflow Specific and sensitive due to ARMS and Scorpions PCR technolog
    Catalog Number:
    870121
    Price:
    6810
    Category:
    therascreen EGFR RGQ PCR Kit
    Buy from Supplier


    Structured Review

    Qiagen therascreen egfr rgq pcr kit
    therascreen EGFR RGQ PCR Kit
    For the confident detection of mutations in the EGFR oncogene Kit contents For 24 reactions 1 Control Assay 7 Mutation Assays Positive Control Water Taq DNA Polymerase Benefits FDA approved for in vitro diagnostic use with GILOTRIF or IRESSA Ready to use system with simple workflow Specific and sensitive due to ARMS and Scorpions PCR technolog
    https://www.bioz.com/result/therascreen egfr rgq pcr kit/product/Qiagen
    Average 99 stars, based on 153 article reviews
    Price from $9.99 to $1999.99
    therascreen egfr rgq pcr kit - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma"

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S132187

    The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.
    Figure Legend Snippet: The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.

    Techniques Used: Polymerase Chain Reaction

    2) Product Images from "Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma"

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma

    Journal: OncoTargets and therapy

    doi: 10.2147/OTT.S132187

    The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.
    Figure Legend Snippet: The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.

    Techniques Used: Polymerase Chain Reaction

    3) Product Images from "The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations"

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations

    Journal: Medicine

    doi: 10.1097/MD.0000000000003991

    The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.
    Figure Legend Snippet: The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    4) Product Images from "Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology"

    Article Title: Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology

    Journal: BioMed Research International

    doi: 10.1155/2013/385087

    Representative results for EGFR mutation screening using direct sequencing, fragment analysis, real-time allelic discrimination, Therascreen EGFR RGQ kit, and EGFR TaqMan Mutation Detection Assays. (a) The right panel is an example of wild type EGFR, the left panel is an example of L858R mutation. (b) The right panel is an example of wild type EGFR, the left panel is an example of a deletion in exon 19 (c.2237_2254del18bp).
    Figure Legend Snippet: Representative results for EGFR mutation screening using direct sequencing, fragment analysis, real-time allelic discrimination, Therascreen EGFR RGQ kit, and EGFR TaqMan Mutation Detection Assays. (a) The right panel is an example of wild type EGFR, the left panel is an example of L858R mutation. (b) The right panel is an example of wild type EGFR, the left panel is an example of a deletion in exon 19 (c.2237_2254del18bp).

    Techniques Used: Mutagenesis, Sequencing

    5) Product Images from "The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations"

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations

    Journal: Medicine

    doi: 10.1097/MD.0000000000003991

    The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.
    Figure Legend Snippet: The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    6) Product Images from "Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology"

    Article Title: Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology

    Journal: BioMed Research International

    doi: 10.1155/2013/385087

    Representative results for EGFR mutation screening using direct sequencing, fragment analysis, real-time allelic discrimination, Therascreen EGFR RGQ kit, and EGFR TaqMan Mutation Detection Assays. (a) The right panel is an example of wild type EGFR, the left panel is an example of L858R mutation. (b) The right panel is an example of wild type EGFR, the left panel is an example of a deletion in exon 19 (c.2237_2254del18bp).
    Figure Legend Snippet: Representative results for EGFR mutation screening using direct sequencing, fragment analysis, real-time allelic discrimination, Therascreen EGFR RGQ kit, and EGFR TaqMan Mutation Detection Assays. (a) The right panel is an example of wild type EGFR, the left panel is an example of L858R mutation. (b) The right panel is an example of wild type EGFR, the left panel is an example of a deletion in exon 19 (c.2237_2254del18bp).

    Techniques Used: Mutagenesis, Sequencing

    7) Product Images from "The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations"

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations

    Journal: Medicine

    doi: 10.1097/MD.0000000000003991

    The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.
    Figure Legend Snippet: The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    8) Product Images from "The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations"

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations

    Journal: Medicine

    doi: 10.1097/MD.0000000000003991

    The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.
    Figure Legend Snippet: The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    9) Product Images from "The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations"

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations

    Journal: Medicine

    doi: 10.1097/MD.0000000000003991

    The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.
    Figure Legend Snippet: The association of the percentage of EGFR mutant DNA and delta Ct determined by the Therascreen EGFR RGQ PCR kit (Qiagen) in lung cancer cells. DNA = deoxyribonucleic acid, EGFR = epidermal growth factor.

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    Related Articles

    Diagnostic Assay:

    Article Title: Real-Time Bidirectional Pyrophosphorolysis-Activated Polymerization for Quantitative Detection of Somatic Mutations
    Article Snippet: .. For comparison, these samples were detected by two commercial diagnostic kits AmoyDx EGFR (Amoy Diagnostics Inc) and TheraScreen EGFR RGQ PCR (Qiagen), both of which are real-time ARMS PCR assays. .. For confirmation, all these samples were analyzed by DNA sequencing using the following primers: 5′-CTTCTTCCCATGATGATCTGTC-3′ (forward) and 5′-AACAATACAGCTAGTGGGAAGG-3′ (reverse) for L858R; 5′-CGTAAACGTCCCTGTGCTA-3′ (forward) and 5′-CAGACCGCATGTGAGGAT-3′ (reverse) for T790M.

    Polymerase Chain Reaction:

    Article Title: Real-Time Bidirectional Pyrophosphorolysis-Activated Polymerization for Quantitative Detection of Somatic Mutations
    Article Snippet: .. For comparison, these samples were detected by two commercial diagnostic kits AmoyDx EGFR (Amoy Diagnostics Inc) and TheraScreen EGFR RGQ PCR (Qiagen), both of which are real-time ARMS PCR assays. .. For confirmation, all these samples were analyzed by DNA sequencing using the following primers: 5′-CTTCTTCCCATGATGATCTGTC-3′ (forward) and 5′-AACAATACAGCTAGTGGGAAGG-3′ (reverse) for L858R; 5′-CGTAAACGTCCCTGTGCTA-3′ (forward) and 5′-CAGACCGCATGTGAGGAT-3′ (reverse) for T790M.

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations
    Article Snippet: .. EGFR mutation status in 18, 19, 20, and 21 exons of the EGFR gene were determined by Therascreen EGFR RGQ PCR kit (Qiagen). ..

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma
    Article Snippet: .. Mutation assay The Therascreen EGFR RGQ PCR kit (Qiagen) detects a total of 29 ( ) specific somatic mutations in exons 18–21 of the EGFR gene utilizing both ARMS® and Scorpion® technologies. .. Data analysis was performed by using Rotor gene proprietary software, and the mutation status was categorized qualitatively as positive and negative based on the Therascreen EGFR RGQ PCR kit guidelines.

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma
    Article Snippet: .. The present study used the Therascreen EGFR RGQ PCR kit for the detection of specific somatic mutations in the EGFR oncogene. .. This method detects mutation as low as 0.5% in the background of wild-type DNA.

    Article Title: Epidermal growth factor receptor (EGFR), KRAS, and BRAF mutations in lung adenocarcinomas: A study from India
    Article Snippet: .. The EGFR RGQ PCR Kit (cat no. 870111, Therascreen, Qiagen Ltd, Manchester, UK) was used for detecting the presence of 29 EGFR mutations spanning exons 18–21. .. KRAS PCR Kit (cat no. 870001 Therascreen, Qiagen Ltd, Manchester, UK) was used for detecting 7 KRAS mutations in exon 2 (codons 12 and 13).

    Article Title: Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology
    Article Snippet: .. Therascreen EGFR RGQ PCR Kit The Therascreen EGFR RGQ PCR kit (Qiagen) allows the detection of 29 somatic mutations in the EGFR oncogene by combining Scorpions and ARMS technologies. .. Samples were processed according to the manufacturer's protocol, using the Rotor-Gene Q real-time PCR cycler (Qiagen).

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma
    Article Snippet: .. Control assay As per the Therascreen® EGFR-Rotor-Gene Q (RGQ) polymerase chain reaction (PCR) kit (Qiagen, Hilden, Germany) mandate, the total amplifiable DNA for each sample was assessed on RGQ real-time PCR (Qiagen) using the control reaction mix. .. Control assay amplifies the exon 2 region of the EGFR gene.

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations
    Article Snippet: .. EGFR mutation analysis for the mixed DNA samples was performed using the Therascreen EGFR RGQ PCR kit (Qiagen) according to manufacturer's manual. .. [ ] Delta Ct (dCt) is calculated as the difference between the mutation assay Ct and control assay Ct from the same sample.

    Real-time Polymerase Chain Reaction:

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma
    Article Snippet: .. Control assay As per the Therascreen® EGFR-Rotor-Gene Q (RGQ) polymerase chain reaction (PCR) kit (Qiagen, Hilden, Germany) mandate, the total amplifiable DNA for each sample was assessed on RGQ real-time PCR (Qiagen) using the control reaction mix. .. Control assay amplifies the exon 2 region of the EGFR gene.

    Mutagenesis:

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations
    Article Snippet: .. EGFR mutation status in 18, 19, 20, and 21 exons of the EGFR gene were determined by Therascreen EGFR RGQ PCR kit (Qiagen). ..

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma
    Article Snippet: .. Mutation assay The Therascreen EGFR RGQ PCR kit (Qiagen) detects a total of 29 ( ) specific somatic mutations in exons 18–21 of the EGFR gene utilizing both ARMS® and Scorpion® technologies. .. Data analysis was performed by using Rotor gene proprietary software, and the mutation status was categorized qualitatively as positive and negative based on the Therascreen EGFR RGQ PCR kit guidelines.

    Article Title: The content of mutant EGFR DNA correlates with response to EGFR-TKIs in lung adenocarcinoma patients with common EGFR mutations
    Article Snippet: .. EGFR mutation analysis for the mixed DNA samples was performed using the Therascreen EGFR RGQ PCR kit (Qiagen) according to manufacturer's manual. .. [ ] Delta Ct (dCt) is calculated as the difference between the mutation assay Ct and control assay Ct from the same sample.

    Control Assay:

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma
    Article Snippet: .. Control assay As per the Therascreen® EGFR-Rotor-Gene Q (RGQ) polymerase chain reaction (PCR) kit (Qiagen, Hilden, Germany) mandate, the total amplifiable DNA for each sample was assessed on RGQ real-time PCR (Qiagen) using the control reaction mix. .. Control assay amplifies the exon 2 region of the EGFR gene.

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  • 99
    Qiagen egfr rgq pcr kit
    Real-time <t>PCR</t> analysis of <t>EGFR</t> and KRAS mutations in pulmonary adenocarcinoma. (a) EGFR control assay. (b) EGFR exon 19 deletion. (c) EGFR exon 19 deletion and exon 21 L858R dual mutation. (d) KRAS control assay. (e) KRAS exon 2 G12A point mutation. (f) KRAS exon 2 G12V and G12C dual mutation.
    Egfr Rgq Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr rgq pcr kit/product/Qiagen
    Average 99 stars, based on 161 article reviews
    Price from $9.99 to $1999.99
    egfr rgq pcr kit - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    Real-time PCR analysis of EGFR and KRAS mutations in pulmonary adenocarcinoma. (a) EGFR control assay. (b) EGFR exon 19 deletion. (c) EGFR exon 19 deletion and exon 21 L858R dual mutation. (d) KRAS control assay. (e) KRAS exon 2 G12A point mutation. (f) KRAS exon 2 G12V and G12C dual mutation.

    Journal: Current problems in cancer

    Article Title: Epidermal growth factor receptor (EGFR), KRAS, and BRAF mutations in lung adenocarcinomas: A study from India

    doi: 10.1016/j.currproblcancer.2018.12.003

    Figure Lengend Snippet: Real-time PCR analysis of EGFR and KRAS mutations in pulmonary adenocarcinoma. (a) EGFR control assay. (b) EGFR exon 19 deletion. (c) EGFR exon 19 deletion and exon 21 L858R dual mutation. (d) KRAS control assay. (e) KRAS exon 2 G12A point mutation. (f) KRAS exon 2 G12V and G12C dual mutation.

    Article Snippet: The EGFR RGQ PCR Kit (cat no. 870111, Therascreen, Qiagen Ltd, Manchester, UK) was used for detecting the presence of 29 EGFR mutations spanning exons 18–21.

    Techniques: Real-time Polymerase Chain Reaction, Control Assay, Mutagenesis

    The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.

    Journal: OncoTargets and therapy

    Article Title: Tyrosine kinase domain mutations of EGFR gene in head and neck squamous cell carcinoma

    doi: 10.2147/OTT.S132187

    Figure Lengend Snippet: The somatic mutations of EGFR detected by Therascreen ® EGFR RGQ PCR kit. Notes: The schematic representation of all 29 mutations detected by the Therascreen EGFR RGQ PCR kit and the frequency detected in the present study. The mutations are color coded based on the respective exons. All somatic mutations on exon 18 are coded in shades of blue; exon 19 mutations are coded in shades of gray; exon 20 mutations are coded in shades of orange; and exon 21 mutations are coded in shades of green. Abbreviations: EGFR, epidermal growth factor receptor; RGQ, Rotor-Gene Q; PCR, polymerase chain reaction.

    Article Snippet: Control assay As per the Therascreen® EGFR-Rotor-Gene Q (RGQ) polymerase chain reaction (PCR) kit (Qiagen, Hilden, Germany) mandate, the total amplifiable DNA for each sample was assessed on RGQ real-time PCR (Qiagen) using the control reaction mix.

    Techniques: Polymerase Chain Reaction