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U73122 PLC thapsigargin
Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM <t>thapsigargin)</t> ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p
Thapsigargin, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thapsigargin/product/U73122 PLC
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
thapsigargin - by Bioz Stars, 2020-11
92/100 stars

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1) Product Images from "P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells"

Article Title: P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells

Journal: Purinergic Signalling

doi: 10.1007/s11302-018-9618-3

Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p
Figure Legend Snippet: Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p

Techniques Used: Planar Chromatography, Activity Assay, Derivative Assay, Inhibition

2) Product Images from "P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells"

Article Title: P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells

Journal: Purinergic Signalling

doi: 10.1007/s11302-018-9618-3

Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p
Figure Legend Snippet: Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p

Techniques Used: Planar Chromatography, Activity Assay, Derivative Assay, Inhibition

Related Articles

other:

Article Title: P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells
Article Snippet: Selective antagonists were obtained from Tocris Bioscience (Bristol, UK) (P2Y1 MRS2500; P2Y2 AR-C118925XX; P2Y6 MRS2578; P2Y11 NF340; P2Y12 PSB-0739; P2Y13 MRS2211; P2X4 PSB12062; P2X7 A438079), excluding thapsigargin (sarco-endoplasmic reticulum Ca2+ -ATPases, SERCA) and U73122 (PLC) (Santa Cruz Biotechnology, Texas, USA).

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    U73122 PLC thapsigargin
    Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM <t>thapsigargin)</t> ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p
    Thapsigargin, supplied by U73122 PLC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin/product/U73122 PLC
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    thapsigargin - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

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    Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p

    Journal: Purinergic Signalling

    Article Title: P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells

    doi: 10.1007/s11302-018-9618-3

    Figure Lengend Snippet: Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p

    Article Snippet: Selective antagonists were obtained from Tocris Bioscience (Bristol, UK) (P2Y1 MRS2500; P2Y2 AR-C118925XX; P2Y6 MRS2578; P2Y11 NF340; P2Y12 PSB-0739; P2Y13 MRS2211; P2X4 PSB12062; P2X7 A438079), excluding thapsigargin (sarco-endoplasmic reticulum Ca2+ -ATPases, SERCA) and U73122 (PLC) (Santa Cruz Biotechnology, Texas, USA).

    Techniques: Planar Chromatography, Activity Assay, Derivative Assay, Inhibition

    Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p

    Journal: Purinergic Signalling

    Article Title: P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells

    doi: 10.1007/s11302-018-9618-3

    Figure Lengend Snippet: Dependency of ATP-elicited intracellular Ca 2+ responses on Ca 2+ influx, PLC activity and release of Ca 2+ from intracellular stores in human adipose-derived mesenchymal stromal cells. a ATP dose-response curve for intracellular Ca 2+ responses in the presence ( closed circles ) and absence ( open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). b ATP dose-response curve under control conditions ( closed circles ) or following phospholipase C inhibition (10 μM U73122) ( N = 6). c ATP dose-response curve under control conditions ( closed circles ) or following sarco-endoplasmic reticulum Ca 2+ -ATPases inhibition induced emptying of the intracellular Ca 2+ stores (5 μM thapsigargin) ( open circles ) ( N = 6). d Average time-resolved trace showing responses elicited by 30 μM ATP in the presence ( closed circles ) and absence (open circles ) of 1.5 mM extracellular Ca 2+ ( N = 6). e Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following U73122 treatment ( open circles ) ( N = 6). f Average time-resolved traces for the response to 30 μM ATP under control conditions ( closed circles ) and following thapsigargin treatment ( open circles ) ( N = 6). Data points are mean±SEM. * p

    Article Snippet: Selective antagonists were obtained from Tocris Bioscience (Bristol, UK) (P2Y1 MRS2500; P2Y2 AR-C118925XX; P2Y6 MRS2578; P2Y11 NF340; P2Y12 PSB-0739; P2Y13 MRS2211; P2X4 PSB12062; P2X7 A438079), excluding thapsigargin (sarco-endoplasmic reticulum Ca2+ -ATPases, SERCA) and U73122 (PLC) (Santa Cruz Biotechnology, Texas, USA).

    Techniques: Planar Chromatography, Activity Assay, Derivative Assay, Inhibition