Journal: International Journal of Molecular Sciences

Article Title: UTRs and Ago-2/miR-335 Complex Restricts Amylin Translation in Insulinoma and Human Pancreatic β-Cells

doi: 10.3390/ijms25179614

Figure Lengend Snippet: miRNAs expression analysis in HG-challenged human islets. Freshly isolated non-diabetic human islets were cultured in the presence of high (20 mM) glucose (HG) or 1 μM thapsigargin (TG) for 24 h. Relative miRNA levels were analyzed by RT-qPCR and changes in miRNA expression levels in treatments, after normalizing to a ubiquitously expressed miRNA SNORD44, were expressed as a fold change (FC) relative to control (normal glucose, NG) cells. Significance established at # p < 0.05, n = 3 (HG vs. NG), and * p < 0.05, *** p < 0.001, n = 3 (TG vs. NG), ANOVA followed by Tukey post hoc comparison test.

Article Snippet: Transfected cells were then treated with vehicle (DMSO) or 1 μM of thapsigargin (TG) for an additional 8 h. Relative firefly and renilla luciferase activities were detected using the Dual-Glo luciferase assay system (Promega, Madison, WI, USA) following the manufacturer’s instructions.

Techniques: Expressing, Isolation, Cell Culture, Quantitative RT-PCR, Control, Comparison