thapsigargin tg  (Selleck Chemicals)


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    Selleck Chemicals thapsigargin tg
    IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and <t>thapsigargin</t> (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-09
    94/100 stars

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    1) Product Images from "IRE1α Expedites the Progression of Castration-Resistant Prostate Cancers via the Positive Feedback Loop of IRE1α/IL-6/AR"

    Article Title: IRE1α Expedites the Progression of Castration-Resistant Prostate Cancers via the Positive Feedback Loop of IRE1α/IL-6/AR

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2021.671141

    IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and thapsigargin (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.
    Figure Legend Snippet: IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and thapsigargin (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.

    Techniques Used: Over Expression, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

    thapsigargin tg  (Selleck Chemicals)


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    Selleck Chemicals thapsigargin tg
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-09
    86/100 stars

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    thapsigargin tg  (Selleck Chemicals)


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    Structured Review

    Selleck Chemicals thapsigargin tg
    IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and <t>thapsigargin</t> (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-09
    94/100 stars

    Images

    1) Product Images from "IRE1α Expedites the Progression of Castration-Resistant Prostate Cancers via the Positive Feedback Loop of IRE1α/IL-6/AR"

    Article Title: IRE1α Expedites the Progression of Castration-Resistant Prostate Cancers via the Positive Feedback Loop of IRE1α/IL-6/AR

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2021.671141

    IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and thapsigargin (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.
    Figure Legend Snippet: IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and thapsigargin (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.

    Techniques Used: Over Expression, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

    thapsigargin tg  (Selleck Chemicals)


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    Selleck Chemicals thapsigargin tg
    Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, <t>thapsigargin</t> (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-09
    94/100 stars

    Images

    1) Product Images from "Inactivation of TFEB and NF- κ B by marchantin M alleviates the chemotherapy-driven pro-tumorigenic senescent secretion"

    Article Title: Inactivation of TFEB and NF- κ B by marchantin M alleviates the chemotherapy-driven pro-tumorigenic senescent secretion

    Journal: Acta Pharmaceutica Sinica. B

    doi: 10.1016/j.apsb.2019.08.007

    Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, thapsigargin (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.
    Figure Legend Snippet: Inactivation of TFEB and NF- κ B contributes to suppression of SASP by Mar-M. (A) Western blot analysis of the P65, the phspho-p65 (p-P65) in PC3/Doc cells treated with Mar-M. (B) Immunoblots of p-P65 state in nuclear and cytosolic fraction of PC3/Doc incubated with Mar-M for 1 or 5 days. GAPDH and Histone 3 (H3) were used as control. (C) The nuclear translocation of P65 was detected by confocal microscopy, LPS was used as positive control. (D) The nuclear and cytoplasmic fraction of TFEB and TFE3 in PC3/Doc cells and PC3 cells. (E) Western blot analysis of the TFEB and TFE3 in PC3/Doc cells treated with Mar-M. (F) Nuclear fraction of TFEB in PC3/Doc cells with Mar-M, thapsigargin (Tg) and Starvation (Sv). (G) Immunofluorescent staining of TFEB-GFP detected in 293T cells treated with Mar-M for 24 h and stained with DAPI (to visualize nuclei). Starvation and thapsigargin were used as positive controls for TFEB nuclear translation. (H) siRNA knockdown of TFEB in PC3/Doc cells was performed and analysed the IL-1α 、 IL-1β and IL-6 levels. (I) Quantification of IL-1α 、 IL-1β and IL-6 levels were shown after TFEB overexpression in 293T cell treated with Mar-M. Results are representative of three independent experiments. Data are mean ± SD, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bar: 20 μm.

    Techniques Used: Western Blot, Incubation, Translocation Assay, Confocal Microscopy, Positive Control, Staining, Over Expression

    thapsigargin tg  (Selleck Chemicals)


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    Selleck Chemicals thapsigargin tg
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-09
    86/100 stars

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    thapsigargin tg  (Selleck Chemicals)


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    Selleck Chemicals thapsigargin tg
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-09
    86/100 stars

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    Selleck Chemicals thapsigargin tg
    IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and <t>thapsigargin</t> (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.
    Thapsigargin Tg, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thapsigargin tg/product/Selleck Chemicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thapsigargin tg - by Bioz Stars, 2023-09
    94/100 stars
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    IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and thapsigargin (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.

    Journal: Frontiers in Oncology

    Article Title: IRE1α Expedites the Progression of Castration-Resistant Prostate Cancers via the Positive Feedback Loop of IRE1α/IL-6/AR

    doi: 10.3389/fonc.2021.671141

    Figure Lengend Snippet: IRE1α stimulates IL-6 production via the IRE1α/XBP-1s pathway in prostate cancer cells. (A) LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown were cultured in an androgen deprivation medium with CSS for 48 h, and mRNA levels of IL-6 were determined by qPCR. (B, C) ELISA and western blot analysis for the protein expression of IL-6 in LNCaP cells with IRE1α overexpression or C4-2B cells with IRE1α knockdown. (D–F) MKC8866 and thapsigargin (TG), the inhibitor and the agonist of the IRE1α/XBP-1s pathway, were used to detect the effect of the IRE1α/XBP-1s pathway on IL-6 expression. Data shown are the mean ± SD from three independent experiments, ** P < 0.01. ns, no significance.

    Article Snippet: MKC8866 and thapsigargin (TG) were purchased from Selleck Chemicals (Shanghai, China).

    Techniques: Over Expression, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing