ttx tetrodotoxin  (Alomone Labs)


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    Alomone Labs ttx tetrodotoxin
    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence <t>of</t> <t>CNQX</t> (10 µM), AP-5 (50 µM) and <t>TTX</t> (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.
    Ttx Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ttx tetrodotoxin - by Bioz Stars, 2023-02
    97/100 stars

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    1) Product Images from "Neuroligin-1 Loss Is Associated with Reduced Tenacity of Excitatory Synapses"

    Article Title: Neuroligin-1 Loss Is Associated with Reduced Tenacity of Excitatory Synapses

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0042314

    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence of CNQX (10 µM), AP-5 (50 µM) and TTX (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.
    Figure Legend Snippet: A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence of CNQX (10 µM), AP-5 (50 µM) and TTX (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.

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    ttx tetrodotoxin  (Alomone Labs)


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    Alomone Labs ttx tetrodotoxin
    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence <t>of</t> <t>CNQX</t> (10 µM), AP-5 (50 µM) and <t>TTX</t> (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.
    Ttx Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ttx tetrodotoxin - by Bioz Stars, 2023-02
    97/100 stars

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    1) Product Images from "Neuroligin-1 Loss Is Associated with Reduced Tenacity of Excitatory Synapses"

    Article Title: Neuroligin-1 Loss Is Associated with Reduced Tenacity of Excitatory Synapses

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0042314

    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence of CNQX (10 µM), AP-5 (50 µM) and TTX (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.
    Figure Legend Snippet: A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence of CNQX (10 µM), AP-5 (50 µM) and TTX (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.

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    tetrodotoxin  (Alomone Labs)


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    Alomone Labs tetrodotoxin
    Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ttx  (Alomone Labs)


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    Alomone Labs ttx
    Ttx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ttx  (Alomone Labs)


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    Alomone Labs ttx
    Ttx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tetrodotoxin  (Alomone Labs)


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    Alomone Labs tetrodotoxin
    Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tetrodotoxin  (Alomone Labs)


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    Alomone Labs tetrodotoxin
    Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ttx  (Alomone Labs)


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    Alomone Labs ttx
    Role of neuronal activity <t>in</t> <t>k-252a's</t> effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), <t>TTX</t> (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Ttx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology"

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    Journal: Neural Plasticity

    doi: 10.1155/2012/578057

    Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Figure Legend Snippet: Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Techniques Used: Activity Assay

    tetrodotoxin  (Alomone Labs)


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    Alomone Labs tetrodotoxin
    Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tetrodotoxin ttx  (Alomone Labs)


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    Alomone Labs tetrodotoxin ttx
    Upregulation of SARE identified candidates in primary cortical neurons. Primary neurons from E18 cortex were cultivated for 16 days before <t>triggering</t> <t>neuronal</t> activation with 4AP/bicuculline. RNA was obtained and transcript expression of SARE containing genes analyzed by quantitative real time RT-PCR (Q-PCR). Expression of each gene is normalized to the expression in control cells. Arc up-regulation demonstrated efficient neuronal activation. * indicates p<0,5 **<0,1, ***<0,05 (t-Student test). B). Novel SARE sequences activate transcription in response to neuronal activity. Mouse genomic sequence containing SARE regulatory regions (see below) corresponding to those identified for the Cux1 and Cux2 genes were cloned into the pGL4.23 luciferase vector (Promega). Neurons obtained from E18,5 cortex were co-transfected with the indicated firefly luciferase reporter construct and internal control Renilla luciferase plasmid at a ratio of 4∶1. Neuronal activity was trigered with 4AP/bicuculline before measuring transcription of the reporters. Relative expression of each reporter constructs was determined by normalizing the activity of each reporter to its activity on <t>TTX</t> treated neurons. Data represent mean and standar deviation of results obtained in three different experiments. * indicates p<0,01, **<0,001 (t-Student test).
    Tetrodotoxin Ttx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Enrichment of Conserved Synaptic Activity-Responsive Element in Neuronal Genes Predicts a Coordinated Response of MEF2, CREB and SRF"

    Article Title: Enrichment of Conserved Synaptic Activity-Responsive Element in Neuronal Genes Predicts a Coordinated Response of MEF2, CREB and SRF

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0053848

    Upregulation of SARE identified candidates in primary cortical neurons. Primary neurons from E18 cortex were cultivated for 16 days before triggering neuronal activation with 4AP/bicuculline. RNA was obtained and transcript expression of SARE containing genes analyzed by quantitative real time RT-PCR (Q-PCR). Expression of each gene is normalized to the expression in control cells. Arc up-regulation demonstrated efficient neuronal activation. * indicates p<0,5 **<0,1, ***<0,05 (t-Student test). B). Novel SARE sequences activate transcription in response to neuronal activity. Mouse genomic sequence containing SARE regulatory regions (see below) corresponding to those identified for the Cux1 and Cux2 genes were cloned into the pGL4.23 luciferase vector (Promega). Neurons obtained from E18,5 cortex were co-transfected with the indicated firefly luciferase reporter construct and internal control Renilla luciferase plasmid at a ratio of 4∶1. Neuronal activity was trigered with 4AP/bicuculline before measuring transcription of the reporters. Relative expression of each reporter constructs was determined by normalizing the activity of each reporter to its activity on TTX treated neurons. Data represent mean and standar deviation of results obtained in three different experiments. * indicates p<0,01, **<0,001 (t-Student test).
    Figure Legend Snippet: Upregulation of SARE identified candidates in primary cortical neurons. Primary neurons from E18 cortex were cultivated for 16 days before triggering neuronal activation with 4AP/bicuculline. RNA was obtained and transcript expression of SARE containing genes analyzed by quantitative real time RT-PCR (Q-PCR). Expression of each gene is normalized to the expression in control cells. Arc up-regulation demonstrated efficient neuronal activation. * indicates p<0,5 **<0,1, ***<0,05 (t-Student test). B). Novel SARE sequences activate transcription in response to neuronal activity. Mouse genomic sequence containing SARE regulatory regions (see below) corresponding to those identified for the Cux1 and Cux2 genes were cloned into the pGL4.23 luciferase vector (Promega). Neurons obtained from E18,5 cortex were co-transfected with the indicated firefly luciferase reporter construct and internal control Renilla luciferase plasmid at a ratio of 4∶1. Neuronal activity was trigered with 4AP/bicuculline before measuring transcription of the reporters. Relative expression of each reporter constructs was determined by normalizing the activity of each reporter to its activity on TTX treated neurons. Data represent mean and standar deviation of results obtained in three different experiments. * indicates p<0,01, **<0,001 (t-Student test).

    Techniques Used: Activation Assay, Expressing, Quantitative RT-PCR, Activity Assay, Sequencing, Clone Assay, Luciferase, Plasmid Preparation, Transfection, Construct

    tetrodotoxin  (Alomone Labs)


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    Alomone Labs tetrodotoxin
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    Alomone Labs ttx tetrodotoxin
    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence <t>of</t> <t>CNQX</t> (10 µM), AP-5 (50 µM) and <t>TTX</t> (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.
    Ttx Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs tetrodotoxin
    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence <t>of</t> <t>CNQX</t> (10 µM), AP-5 (50 µM) and <t>TTX</t> (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.
    Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs ttx
    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence <t>of</t> <t>CNQX</t> (10 µM), AP-5 (50 µM) and <t>TTX</t> (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.
    Ttx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs tetrodotoxin ttx
    Upregulation of SARE identified candidates in primary cortical neurons. Primary neurons from E18 cortex were cultivated for 16 days before <t>triggering</t> <t>neuronal</t> activation with 4AP/bicuculline. RNA was obtained and transcript expression of SARE containing genes analyzed by quantitative real time RT-PCR (Q-PCR). Expression of each gene is normalized to the expression in control cells. Arc up-regulation demonstrated efficient neuronal activation. * indicates p<0,5 **<0,1, ***<0,05 (t-Student test). B). Novel SARE sequences activate transcription in response to neuronal activity. Mouse genomic sequence containing SARE regulatory regions (see below) corresponding to those identified for the Cux1 and Cux2 genes were cloned into the pGL4.23 luciferase vector (Promega). Neurons obtained from E18,5 cortex were co-transfected with the indicated firefly luciferase reporter construct and internal control Renilla luciferase plasmid at a ratio of 4∶1. Neuronal activity was trigered with 4AP/bicuculline before measuring transcription of the reporters. Relative expression of each reporter constructs was determined by normalizing the activity of each reporter to its activity on <t>TTX</t> treated neurons. Data represent mean and standar deviation of results obtained in three different experiments. * indicates p<0,01, **<0,001 (t-Student test).
    Tetrodotoxin Ttx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence of CNQX (10 µM), AP-5 (50 µM) and TTX (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.

    Journal: PLoS ONE

    Article Title: Neuroligin-1 Loss Is Associated with Reduced Tenacity of Excitatory Synapses

    doi: 10.1371/journal.pone.0042314

    Figure Lengend Snippet: A ) Distribution of range over mean values for SEpH:GluA2 puncta in the presence of CNQX (10 µM), AP-5 (50 µM) and TTX (1 µM) (Nlgn-1 KO: 10 neurons, 481 synapses; WT: 10 neurons, 514 synapses). B ) SI decay rates in the presence of the aforementioned pharmacological agents.

    Article Snippet: Reagents were procured from the following sources: CNQX (6-cyano-7 nitroquinoxaline-2,3-dione) from Tocris Bioscience; AP-5 (2-amino-5-phosphonopentanoic acid) from Sigma-Aldrich; TTX (tetrodotoxin) from Alomone Labs. A mixture of CNQX, AP-5, and TTX were diluted in 100 µl medium drawn from the cloning cylinder in which the neurons were grown.

    Techniques:

    Upregulation of SARE identified candidates in primary cortical neurons. Primary neurons from E18 cortex were cultivated for 16 days before triggering neuronal activation with 4AP/bicuculline. RNA was obtained and transcript expression of SARE containing genes analyzed by quantitative real time RT-PCR (Q-PCR). Expression of each gene is normalized to the expression in control cells. Arc up-regulation demonstrated efficient neuronal activation. * indicates p<0,5 **<0,1, ***<0,05 (t-Student test). B). Novel SARE sequences activate transcription in response to neuronal activity. Mouse genomic sequence containing SARE regulatory regions (see below) corresponding to those identified for the Cux1 and Cux2 genes were cloned into the pGL4.23 luciferase vector (Promega). Neurons obtained from E18,5 cortex were co-transfected with the indicated firefly luciferase reporter construct and internal control Renilla luciferase plasmid at a ratio of 4∶1. Neuronal activity was trigered with 4AP/bicuculline before measuring transcription of the reporters. Relative expression of each reporter constructs was determined by normalizing the activity of each reporter to its activity on TTX treated neurons. Data represent mean and standar deviation of results obtained in three different experiments. * indicates p<0,01, **<0,001 (t-Student test).

    Journal: PLoS ONE

    Article Title: Enrichment of Conserved Synaptic Activity-Responsive Element in Neuronal Genes Predicts a Coordinated Response of MEF2, CREB and SRF

    doi: 10.1371/journal.pone.0053848

    Figure Lengend Snippet: Upregulation of SARE identified candidates in primary cortical neurons. Primary neurons from E18 cortex were cultivated for 16 days before triggering neuronal activation with 4AP/bicuculline. RNA was obtained and transcript expression of SARE containing genes analyzed by quantitative real time RT-PCR (Q-PCR). Expression of each gene is normalized to the expression in control cells. Arc up-regulation demonstrated efficient neuronal activation. * indicates p<0,5 **<0,1, ***<0,05 (t-Student test). B). Novel SARE sequences activate transcription in response to neuronal activity. Mouse genomic sequence containing SARE regulatory regions (see below) corresponding to those identified for the Cux1 and Cux2 genes were cloned into the pGL4.23 luciferase vector (Promega). Neurons obtained from E18,5 cortex were co-transfected with the indicated firefly luciferase reporter construct and internal control Renilla luciferase plasmid at a ratio of 4∶1. Neuronal activity was trigered with 4AP/bicuculline before measuring transcription of the reporters. Relative expression of each reporter constructs was determined by normalizing the activity of each reporter to its activity on TTX treated neurons. Data represent mean and standar deviation of results obtained in three different experiments. * indicates p<0,01, **<0,001 (t-Student test).

    Article Snippet: 12 h before inducing neuronal activity cells were incubated with 2 mM tetrodotoxin (TTX) (Alomone-labs, Jerusalem, Israel).

    Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Activity Assay, Sequencing, Clone Assay, Luciferase, Plasmid Preparation, Transfection, Construct