tetramethylammonium chloride  (Millipore)


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    Name:
    Tetramethylammonium chloride solution
    Description:

    Catalog Number:
    t3411
    Price:
    None
    Applications:
    Tetramethylammonium binds AT-rich DNA polymers while concomitantly abolishing the preferential melting of AT versus GC base pairs. Supplied as 0.2 mum filtered solution in 18 megohm water.
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    Structured Review

    Millipore tetramethylammonium chloride
    Tetramethylammonium chloride solution

    https://www.bioz.com/result/tetramethylammonium chloride/product/Millipore
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    tetramethylammonium chloride - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Oligonucleotide Microarray for 16S rRNA Gene-Based Detection of All Recognized Lineages of Sulfate-Reducing Prokaryotes in the Environment
    Article Snippet: .. Additionally, 20 mM tetramethylammonium chloride (Sigma, Deisenhofen, Germany) was added to each amplification mixture to enhance the specificity of the PCR ( ). ..

    Article Title: Critical Role of the UBL Domain in Stimulating the E3 Ubiquitin Ligase Activity of UHRF1 toward Chromatin
    Article Snippet: .. The amplification of bisulfite converted DNA was performed in 25 μL PCR reaction volumes containing 0.4 μM each of forward and reverse primers, 2 mM Betaine (Sigma-Aldrich, B0300-1VL), 10 mM Tetramethylammonium chloride solution (Sigma-Aldrich T3411-500ML), 1x MyTaq Reaction Buffer, 0.5 units of MyTaq HS (Bioline, BIO-21112), and 1 μL of the eluted bisulfite converted DNA (∼12.5 ng). .. The following cycling parameters were used: 5 min for 95°C for initial denaturation and activation of the polymerase, 40 cycles (95°C for 20 s, 58°C for 30 s, 72°C for 25 s) and a final elongation at 72°C for 3 min. Agarose gel electrophoresis was used to determine the quality and yield of the PCR.

    Article Title: Detection of Clonal and Subclonal Copy-Number Variants in Cell-Free DNA from Patients with Breast Cancer Using a Massively Multiplexed PCR Methodology
    Article Snippet: .. The mmPCR reaction contained Qiagen Multiplex PCR Master Mix, 70 mM tetramethylammonium chloride (Sigma), 6 μM primers, and 7 μl of library. .. Next, sequencing tags and index sequences were added in a barcoding PCR reaction, as described previously .

    Multiplex Assay:

    Article Title: Detection of Clonal and Subclonal Copy-Number Variants in Cell-Free DNA from Patients with Breast Cancer Using a Massively Multiplexed PCR Methodology
    Article Snippet: .. The mmPCR reaction contained Qiagen Multiplex PCR Master Mix, 70 mM tetramethylammonium chloride (Sigma), 6 μM primers, and 7 μl of library. .. Next, sequencing tags and index sequences were added in a barcoding PCR reaction, as described previously .

    Amplification:

    Article Title: Oligonucleotide Microarray for 16S rRNA Gene-Based Detection of All Recognized Lineages of Sulfate-Reducing Prokaryotes in the Environment
    Article Snippet: .. Additionally, 20 mM tetramethylammonium chloride (Sigma, Deisenhofen, Germany) was added to each amplification mixture to enhance the specificity of the PCR ( ). ..

    Article Title: Critical Role of the UBL Domain in Stimulating the E3 Ubiquitin Ligase Activity of UHRF1 toward Chromatin
    Article Snippet: .. The amplification of bisulfite converted DNA was performed in 25 μL PCR reaction volumes containing 0.4 μM each of forward and reverse primers, 2 mM Betaine (Sigma-Aldrich, B0300-1VL), 10 mM Tetramethylammonium chloride solution (Sigma-Aldrich T3411-500ML), 1x MyTaq Reaction Buffer, 0.5 units of MyTaq HS (Bioline, BIO-21112), and 1 μL of the eluted bisulfite converted DNA (∼12.5 ng). .. The following cycling parameters were used: 5 min for 95°C for initial denaturation and activation of the polymerase, 40 cycles (95°C for 20 s, 58°C for 30 s, 72°C for 25 s) and a final elongation at 72°C for 3 min. Agarose gel electrophoresis was used to determine the quality and yield of the PCR.

    Article Title: Cooperative DNA and histone binding by Uhrf2 links the two major repressive epigenetic pathways
    Article Snippet: .. For amplification we used Qiagen Hot Start Polymerase in 1× Qiagen Hot Start Polymerase buffer supplemented with 0.2 mM dNTPs, 0.2 µM forward primer, 0.2 µM reverse primer, 1.3 mM betaine (Sigma) and 60 mM tetramethylammonium-chloride (TMAC, Sigma). .. Major satellites were amplified in a single amplification and pyrosequencing reactions were carried out by Varionostic GmbH (Ulm, Germany).

    Activity Assay:

    Article Title: Influence of sodium substitutes on 5-HT-mediated effects at mouse 5-HT3 receptors
    Article Snippet: .. The following compounds were used in the experiments (for chemical structures, see ): 5-hydroxytryptamine creatinine sulphate from Sigma, Munich, Germany; [14 C]guanidinium chloride (specific activity=59 mCi mmol−1 ) from CEA (Biotrend, Cologne, Germany); [3 H]GR65630, (specific activity 64.8 Ci mmol−1 ) from NEN DuPont (Dreieich, Germany); choline chloride, TMA chloride, Tris and NMDG were all from Sigma (Munich, Germany). .. Basal [ 14 C]guanidinium influx : In N1E-115 cells incubated in buffer containing 135 m M sodium, basal influx of [14 C]guanidinium, that is, influx in the absence of 5-HT, amounted to 16.0±0.5 pmol × mg protein−1 ( n =19).

    other:

    Article Title: Deletion of aquaporin-4 increases extracellular K+ concentration during synaptic stimulation in mouse hippocampus
    Article Snippet: Stimulation and recording Before the experiments, ion-sensitive electrodes were silanized and filled with 150 mM tetramethylammonium chloride (TMA+ , Sigma Life Sciences).

    Article Title: Efficiency measures the conversion of agonist binding energy into receptor conformational change
    Article Snippet: Chemicals NaCl, KCl, CaCl2 , MgCl2 , HEPES, NaOH, KOH, KH2 PO4 , Na2 HPO4 , ACh chloride, CCh, TMA, Cho, and Ebx were purchased from Sigma.

    Article Title: Solvatochromic parameters of deep eutectic solvents formed by ammonium-based salts and carboxylic acids
    Article Snippet: The investigated ammonium-based deep eutectic solvents were prepared with the following hydrogen bond acceptors: Cholinium Chloride, [N1112(OH) ]Cl (Acros Organics, 98 wt%), Tetramethylammonium Chloride, [N1111 ]Cl (Sigma-Aldrich, 97 wt%), Tetraethylammonium Chloride, [N2222 ]Cl (Sigma-Aldrich, 98.0 wt%), Tetrapropylammonium Chloride, [N3333 ]Cl (Sigma-Aldrich, 98.0 wt%), Tetrabutylammonium Chloride, [N4444 ]Cl (Sigma-Aldrich, 97.0 wt%), Tetramethylammonium Bromide, [N1111 ]Br (Fluka, 99 wt%), Tetraethylammonium Bromide, [N2222 ]Br (Alfa Aesar, 98.0 wt%), Tetrapropylammonium Bromide, [N3333 ]Br (Aldrich, 98.0 wt%), and Tetrabutylammonium Bromide, [N4444 ]Br (Fluka, 98.0 wt%).

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  • 99
    Millipore tetraethylammonium cl
    Perforated-patch whole-cell voltage-clamp measurements from ganglion cell somata. ( A ) Membrane potential (upper traces) and whole-cell current (lower traces) were both measured in discontinuous voltage-clamp mode. The holding potential was −72 mV, and the test potential was increased from −47 to +23 mV, in 5-mV steps. The inset superimposes the measured membrane potential (solid line) over the intended potential (−22 mV; dashed line) and shows that the largest voltage error during all of the recordings from this cell was less than 2.5 mV. Comparison of the voltage and current traces shows that this error occurred during the rising phase of the inward current. The current traces are shown with no leak subtraction. Also, the current from the beginning of each voltage step to 250 μs thereafter are not plotted because membrane capacitive currents cannot be electronically compensated with the amplifier used. ( B ) Current amplitude vs. membrane potential for the data in ( A ). Filled circles plot the maximum inward current at each test potential; open circles plot the maximum outward current at each test potential. ( C ) Voltage-gated Na + current activated at a relatively negative test potential (−52 mV). The holding potential was −72 mV, and the pulse duration was 100 msec. The current trace is the difference between currents before and after application of TTX (1μM). ( D ) Voltage-gated Na + current of relatively large amplitude. The holding potential was −72 mV, and the test potentials were −37, −22 and −7 mV. Peak amplitude of inward Na + current reached approx. 28 nA (lower trace) during the depolarization to −22 mV. Difference between the command potential and membrane potential is 8 mV at most (upper trace). Leak current subtraction was carried out off-line. In C and D , the bath solution contained (in mM): 110 NaCl, 3 CsCl, 30 <t>tetraethylammonium-Cl,</t> 2.4 MgCl 2 , 0.1 CaCl 2 , 10 D-glucose, 5 HEPES. The pH was adjusted to 7.4 with CsOH, and the osmolality was 280 mOsmol/kg. The pipette solution contained (in mM): 140 CsOH, 15 NaCl, 2.6 MgCl 2 , 0.34 CaCl 2 , 1 EGTA, 10 HEPES. The pH was adjusted to 7.4 with methanesulfonic acid, and the osmolality was adjusted with sucrose to 260 mOsmo/kg. The amphotericin B concentration in the patch electrode was as described in Materials and Methods.
    Tetraethylammonium Cl, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tetraethylammonium cl/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    tetraethylammonium cl - by Bioz Stars, 2020-07
    99/100 stars
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    Perforated-patch whole-cell voltage-clamp measurements from ganglion cell somata. ( A ) Membrane potential (upper traces) and whole-cell current (lower traces) were both measured in discontinuous voltage-clamp mode. The holding potential was −72 mV, and the test potential was increased from −47 to +23 mV, in 5-mV steps. The inset superimposes the measured membrane potential (solid line) over the intended potential (−22 mV; dashed line) and shows that the largest voltage error during all of the recordings from this cell was less than 2.5 mV. Comparison of the voltage and current traces shows that this error occurred during the rising phase of the inward current. The current traces are shown with no leak subtraction. Also, the current from the beginning of each voltage step to 250 μs thereafter are not plotted because membrane capacitive currents cannot be electronically compensated with the amplifier used. ( B ) Current amplitude vs. membrane potential for the data in ( A ). Filled circles plot the maximum inward current at each test potential; open circles plot the maximum outward current at each test potential. ( C ) Voltage-gated Na + current activated at a relatively negative test potential (−52 mV). The holding potential was −72 mV, and the pulse duration was 100 msec. The current trace is the difference between currents before and after application of TTX (1μM). ( D ) Voltage-gated Na + current of relatively large amplitude. The holding potential was −72 mV, and the test potentials were −37, −22 and −7 mV. Peak amplitude of inward Na + current reached approx. 28 nA (lower trace) during the depolarization to −22 mV. Difference between the command potential and membrane potential is 8 mV at most (upper trace). Leak current subtraction was carried out off-line. In C and D , the bath solution contained (in mM): 110 NaCl, 3 CsCl, 30 tetraethylammonium-Cl, 2.4 MgCl 2 , 0.1 CaCl 2 , 10 D-glucose, 5 HEPES. The pH was adjusted to 7.4 with CsOH, and the osmolality was 280 mOsmol/kg. The pipette solution contained (in mM): 140 CsOH, 15 NaCl, 2.6 MgCl 2 , 0.34 CaCl 2 , 1 EGTA, 10 HEPES. The pH was adjusted to 7.4 with methanesulfonic acid, and the osmolality was adjusted with sucrose to 260 mOsmo/kg. The amphotericin B concentration in the patch electrode was as described in Materials and Methods.

    Journal: Journal of neuroscience methods

    Article Title: Dissociation of Retinal Ganglion Cells Without Enzymes

    doi: 10.1016/j.jneumeth.2004.02.008

    Figure Lengend Snippet: Perforated-patch whole-cell voltage-clamp measurements from ganglion cell somata. ( A ) Membrane potential (upper traces) and whole-cell current (lower traces) were both measured in discontinuous voltage-clamp mode. The holding potential was −72 mV, and the test potential was increased from −47 to +23 mV, in 5-mV steps. The inset superimposes the measured membrane potential (solid line) over the intended potential (−22 mV; dashed line) and shows that the largest voltage error during all of the recordings from this cell was less than 2.5 mV. Comparison of the voltage and current traces shows that this error occurred during the rising phase of the inward current. The current traces are shown with no leak subtraction. Also, the current from the beginning of each voltage step to 250 μs thereafter are not plotted because membrane capacitive currents cannot be electronically compensated with the amplifier used. ( B ) Current amplitude vs. membrane potential for the data in ( A ). Filled circles plot the maximum inward current at each test potential; open circles plot the maximum outward current at each test potential. ( C ) Voltage-gated Na + current activated at a relatively negative test potential (−52 mV). The holding potential was −72 mV, and the pulse duration was 100 msec. The current trace is the difference between currents before and after application of TTX (1μM). ( D ) Voltage-gated Na + current of relatively large amplitude. The holding potential was −72 mV, and the test potentials were −37, −22 and −7 mV. Peak amplitude of inward Na + current reached approx. 28 nA (lower trace) during the depolarization to −22 mV. Difference between the command potential and membrane potential is 8 mV at most (upper trace). Leak current subtraction was carried out off-line. In C and D , the bath solution contained (in mM): 110 NaCl, 3 CsCl, 30 tetraethylammonium-Cl, 2.4 MgCl 2 , 0.1 CaCl 2 , 10 D-glucose, 5 HEPES. The pH was adjusted to 7.4 with CsOH, and the osmolality was 280 mOsmol/kg. The pipette solution contained (in mM): 140 CsOH, 15 NaCl, 2.6 MgCl 2 , 0.34 CaCl 2 , 1 EGTA, 10 HEPES. The pH was adjusted to 7.4 with methanesulfonic acid, and the osmolality was adjusted with sucrose to 260 mOsmo/kg. The amphotericin B concentration in the patch electrode was as described in Materials and Methods.

    Article Snippet: The following chemicals were obtained from other sources: B-27 (without bovine serum albumin, #99-0254DG; Life Technologies, Grand Island, NY), CaCl2 (#190464K; BDH Laboratory Supplies, Poole, England), CsCl (#813061; ICN Biomedical, Aurora, OH), CsOH (#101328; ICN Biomedical), dimethylsulfoxide (#317275; Calbiochem, La Jolla, CA), L-15 (#41300-039; GIBCO Invitrogen, Grand Island, NY), sodium pentobarbital solution (#NDC 0074-3778-05; Abbott Laboratories, North Chicago, IL), Pluronic F-127 (#P6867; Molecular Probes, Eugene, OR), succinyl-concanavalin A (#L1102-25; EY Laboratories, San Mateo, CA), Sylgard 184 (Dow Corning, Midland, MI), tetraethylammonium-Cl (#584128; Calbiochem), tetrodotoxin (#584411, Calbiochem).

    Techniques: Transferring, Concentration Assay