Structured Review

TaKaRa terra pcr direct polymerase mix
Terra Pcr Direct Polymerase Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/terra pcr direct polymerase mix/product/TaKaRa
Average 93 stars, based on 21 article reviews
Price from $9.99 to $1999.99
terra pcr direct polymerase mix - by Bioz Stars, 2020-05
93/100 stars

Images

Related Articles

Polymerase Chain Reaction:

Article Title: Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis
Article Snippet: .. Mouse genotyping was performed by PCR using Terra PCR Direct Polymerase Mix (Clontech). .. Polymerase chain reaction was used to detect fragments of the wild-type (WT) Fli-1 and Fli-1flox/flox allele, as previously described [ ].

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    TaKaRa terra pcr direct polymerase mix
    Erh1 and Ccr4 regulate heterochromatin formation at rDNA repeats to protect rDNA integrity (A) ChIP-chip of Erh1-GFP (top) and myc-Ago1 (bottom) at rDNA repeats in WT. (B) ChIP-chip of H3K9me2 at rDNA repeats in WT and erh1 Δ (top), and in WT and ccr4 Δ (bottom). (C) ChIP-chip of H3K9me2 at rDNA repeats in WT and red1 Δ. (D) LEU2 and <t>ura4</t> + markers inserted at rDNA repeats (Ylp2.4pUC ura4 + -7) are de-repressed in erh1 Δ and ccr4 Δ. A schematic representation of Ylp2.4pUC ura4 + -7 is shown (top). WT and erh1 Δ (middle) or WT and ccr4 Δ (bottom) carrying Ylp2.4pUC ura4 + -7 were spotted onto complete medium or minimal medium lacking leucine and uracil (-Leu-Ura) and incubated at 30°C for 2 days. (E) Increased frequency of mitotic recombination at tandem rDNA repeats in erh1 Δ and ccr4 Δ. A schematic representation of rDNA :: ura4 + loss is shown (left). WT, erh1 Δ and ccr4 Δ carrying Ylp2.4pUC ura4 + -7 were first plated on medium lacking leucine, transferred to complete medium, and then replica plated to medium lacking uracil (-Ura). Mitotic recombination at rDNA loci was indicated by the lack of growth on -Ura plates, and was confirmed by genomic <t>PCR.</t> “ n .
    Terra Pcr Direct Polymerase Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/terra pcr direct polymerase mix/product/TaKaRa
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    terra pcr direct polymerase mix - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    Erh1 and Ccr4 regulate heterochromatin formation at rDNA repeats to protect rDNA integrity (A) ChIP-chip of Erh1-GFP (top) and myc-Ago1 (bottom) at rDNA repeats in WT. (B) ChIP-chip of H3K9me2 at rDNA repeats in WT and erh1 Δ (top), and in WT and ccr4 Δ (bottom). (C) ChIP-chip of H3K9me2 at rDNA repeats in WT and red1 Δ. (D) LEU2 and ura4 + markers inserted at rDNA repeats (Ylp2.4pUC ura4 + -7) are de-repressed in erh1 Δ and ccr4 Δ. A schematic representation of Ylp2.4pUC ura4 + -7 is shown (top). WT and erh1 Δ (middle) or WT and ccr4 Δ (bottom) carrying Ylp2.4pUC ura4 + -7 were spotted onto complete medium or minimal medium lacking leucine and uracil (-Leu-Ura) and incubated at 30°C for 2 days. (E) Increased frequency of mitotic recombination at tandem rDNA repeats in erh1 Δ and ccr4 Δ. A schematic representation of rDNA :: ura4 + loss is shown (left). WT, erh1 Δ and ccr4 Δ carrying Ylp2.4pUC ura4 + -7 were first plated on medium lacking leucine, transferred to complete medium, and then replica plated to medium lacking uracil (-Ura). Mitotic recombination at rDNA loci was indicated by the lack of growth on -Ura plates, and was confirmed by genomic PCR. “ n .

    Journal: Molecular cell

    Article Title: Enhancer of rudimentary cooperates with conserved RNA processing factors to promote meiotic mRNA decay and facultative heterochromatin assembly

    doi: 10.1016/j.molcel.2016.01.029

    Figure Lengend Snippet: Erh1 and Ccr4 regulate heterochromatin formation at rDNA repeats to protect rDNA integrity (A) ChIP-chip of Erh1-GFP (top) and myc-Ago1 (bottom) at rDNA repeats in WT. (B) ChIP-chip of H3K9me2 at rDNA repeats in WT and erh1 Δ (top), and in WT and ccr4 Δ (bottom). (C) ChIP-chip of H3K9me2 at rDNA repeats in WT and red1 Δ. (D) LEU2 and ura4 + markers inserted at rDNA repeats (Ylp2.4pUC ura4 + -7) are de-repressed in erh1 Δ and ccr4 Δ. A schematic representation of Ylp2.4pUC ura4 + -7 is shown (top). WT and erh1 Δ (middle) or WT and ccr4 Δ (bottom) carrying Ylp2.4pUC ura4 + -7 were spotted onto complete medium or minimal medium lacking leucine and uracil (-Leu-Ura) and incubated at 30°C for 2 days. (E) Increased frequency of mitotic recombination at tandem rDNA repeats in erh1 Δ and ccr4 Δ. A schematic representation of rDNA :: ura4 + loss is shown (left). WT, erh1 Δ and ccr4 Δ carrying Ylp2.4pUC ura4 + -7 were first plated on medium lacking leucine, transferred to complete medium, and then replica plated to medium lacking uracil (-Ura). Mitotic recombination at rDNA loci was indicated by the lack of growth on -Ura plates, and was confirmed by genomic PCR. “ n .

    Article Snippet: Loss of ura4 + was confirmed by PCR using Terra™ PCR Direct Polymerase Mix (TaKaRa Bio).

    Techniques: Chromatin Immunoprecipitation, Incubation, Polymerase Chain Reaction

    Ectopic expression of OsWRKY42 suppresses salt and cellulase induced expression of JA biosynthesis and responsive genes in Arabidopsis transgenic lines. Expression of JA biosynthesis and response genes was measured in 35S::OsWRKY42 transgenic lines and wild type (Col-0) Arabidopsis plants after salt and cellulase treatment. a. For salt treatment, one-week old seedlings (wild type or 35S::OsWRKY42) were treated for 12 h with either MS or MS with 150 mM NaCl. b. For cellulase treatment, leaves of three weeks old Arabidopsis plants (wild type or 35S::OsWRKY42) were infiltrated with 2 U/ml of cellulase (Sigma) . Leaves were harvested after 12 h and processed for qPCR analysis. The graph represents the relative fold change using expression values from wild type or 35S::OsWRKY42 lines treated with water. c. Leaves of fourteen days old rice seedlings were syringe infiltrated with either LBA4404 or LBA4404/pH7FWG2::OsWRKY42. Twelve hours post treatment, leaves were harvested and processed for qRT-PCR. The relative fold change was calculated over control (leaves infiltrated only with LBA4404). AtUBQ5 and OsGAPDH were used as endogenous controls for rice and Arabidopsis, respectively. The average from three biological replicates is plotted on the graph. The error bar represents standard deviation. Data was analysed using the Student’s t -test for independent means. The asterisk above the bar indicates significant difference with p value

    Journal: BMC Plant Biology

    Article Title: Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection

    doi: 10.1186/s12870-018-1391-5

    Figure Lengend Snippet: Ectopic expression of OsWRKY42 suppresses salt and cellulase induced expression of JA biosynthesis and responsive genes in Arabidopsis transgenic lines. Expression of JA biosynthesis and response genes was measured in 35S::OsWRKY42 transgenic lines and wild type (Col-0) Arabidopsis plants after salt and cellulase treatment. a. For salt treatment, one-week old seedlings (wild type or 35S::OsWRKY42) were treated for 12 h with either MS or MS with 150 mM NaCl. b. For cellulase treatment, leaves of three weeks old Arabidopsis plants (wild type or 35S::OsWRKY42) were infiltrated with 2 U/ml of cellulase (Sigma) . Leaves were harvested after 12 h and processed for qPCR analysis. The graph represents the relative fold change using expression values from wild type or 35S::OsWRKY42 lines treated with water. c. Leaves of fourteen days old rice seedlings were syringe infiltrated with either LBA4404 or LBA4404/pH7FWG2::OsWRKY42. Twelve hours post treatment, leaves were harvested and processed for qRT-PCR. The relative fold change was calculated over control (leaves infiltrated only with LBA4404). AtUBQ5 and OsGAPDH were used as endogenous controls for rice and Arabidopsis, respectively. The average from three biological replicates is plotted on the graph. The error bar represents standard deviation. Data was analysed using the Student’s t -test for independent means. The asterisk above the bar indicates significant difference with p value

    Article Snippet: The transgenic nature of the T1 plants was further confirmed by direct PCR (Terra PCR direct polymerase kit, Clontech using leaf tissue and sequencing of the amplified product.

    Techniques: Expressing, Transgenic Assay, Mass Spectrometry, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Standard Deviation

    Ectopic expression of OsWRKY42 enhances tolerance to salt stress in Arabidopsis transgenic lines. a. One-week-old TN-1 rice seedlings (n = 10) were dipped in each of the following: 150 mM NaCl solution, 20% ( w / v ) PEG-6000 and mock (water). Leaves were harvested 12 h post treatment and processed for qRT-PCR. Relative fold change was calculated over water treated samples and OsGAPDH was used as an internal control. b. For assaying salt tolerance, one-week-old Arabidopsis seedlings that are either constitutively expressing OsWRKY42 (35S::OsWRKY42) or wild type (Col-0) ( n = 20) were grown on MS agar medium with or without NaCl (150 mM, Merck). (c-d) The total fresh weight and number of root branches of individual seedlings was quantified on the fifteenth day post-treatment. e . For anthocyanin estimation, three weeks old Arabidopsis plants (wild type and 35S::OsWRKY42) were treated with either 150 mM NaCl or water. On the fifteenth day post treatment, leaves ( n = 3) were collected and anthocyanin was extracted in acidic methanol. Anthocyanin was estimated using a spectrophotometer and the anthocyanin content is expressed as absorbance per milligram of fresh weight (F.W). f . ROS estimation was done in detached leaves from adult plants. Leaf discs (n = 15) of either wild type or 35S::OsWRKY42 lines were placed on sterile filter papers soaked in MS solution either with or without NaCl (150 mM). 12 h post treatment, the leaves were stained with NBT. The O 2 − content was estimated spectrophotometrically and is expressed as change in absorbance per milligram of fresh weight. Leaves from three different plants were used as replicates. The data was analysed using one-way ANOVA and the Tukey-Karmer honestly significance difference test. The alphabets above the bar indicates significant difference with p value

    Journal: BMC Plant Biology

    Article Title: Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection

    doi: 10.1186/s12870-018-1391-5

    Figure Lengend Snippet: Ectopic expression of OsWRKY42 enhances tolerance to salt stress in Arabidopsis transgenic lines. a. One-week-old TN-1 rice seedlings (n = 10) were dipped in each of the following: 150 mM NaCl solution, 20% ( w / v ) PEG-6000 and mock (water). Leaves were harvested 12 h post treatment and processed for qRT-PCR. Relative fold change was calculated over water treated samples and OsGAPDH was used as an internal control. b. For assaying salt tolerance, one-week-old Arabidopsis seedlings that are either constitutively expressing OsWRKY42 (35S::OsWRKY42) or wild type (Col-0) ( n = 20) were grown on MS agar medium with or without NaCl (150 mM, Merck). (c-d) The total fresh weight and number of root branches of individual seedlings was quantified on the fifteenth day post-treatment. e . For anthocyanin estimation, three weeks old Arabidopsis plants (wild type and 35S::OsWRKY42) were treated with either 150 mM NaCl or water. On the fifteenth day post treatment, leaves ( n = 3) were collected and anthocyanin was extracted in acidic methanol. Anthocyanin was estimated using a spectrophotometer and the anthocyanin content is expressed as absorbance per milligram of fresh weight (F.W). f . ROS estimation was done in detached leaves from adult plants. Leaf discs (n = 15) of either wild type or 35S::OsWRKY42 lines were placed on sterile filter papers soaked in MS solution either with or without NaCl (150 mM). 12 h post treatment, the leaves were stained with NBT. The O 2 − content was estimated spectrophotometrically and is expressed as change in absorbance per milligram of fresh weight. Leaves from three different plants were used as replicates. The data was analysed using one-way ANOVA and the Tukey-Karmer honestly significance difference test. The alphabets above the bar indicates significant difference with p value

    Article Snippet: The transgenic nature of the T1 plants was further confirmed by direct PCR (Terra PCR direct polymerase kit, Clontech using leaf tissue and sequencing of the amplified product.

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR, Mass Spectrometry, Spectrophotometry, Staining

    Ectopic expression of OsWRKY42 suppresses salt and cellulase induced expression of JA biosynthesis and responsive genes in Arabidopsis transgenic lines. Expression of JA biosynthesis and response genes was measured in 35S::OsWRKY42 transgenic lines and wild type (Col-0) Arabidopsis plants after salt and cellulase treatment. a. For salt treatment, one-week old seedlings (wild type or 35S::OsWRKY42) were treated for 12 h with either MS or MS with 150 mM NaCl. b. For cellulase treatment, leaves of three weeks old Arabidopsis plants (wild type or 35S::OsWRKY42) were infiltrated with 2 U/ml of cellulase (Sigma) . Leaves were harvested after 12 h and processed for qPCR analysis. The graph represents the relative fold change using expression values from wild type or 35S::OsWRKY42 lines treated with water. c. Leaves of fourteen days old rice seedlings were syringe infiltrated with either LBA4404 or LBA4404/pH7FWG2::OsWRKY42. Twelve hours post treatment, leaves were harvested and processed for qRT-PCR. The relative fold change was calculated over control (leaves infiltrated only with LBA4404). AtUBQ5 and OsGAPDH were used as endogenous controls for rice and Arabidopsis, respectively. The average from three biological replicates is plotted on the graph. The error bar represents standard deviation. Data was analysed using the Student’s t -test for independent means. The asterisk above the bar indicates significant difference with p value

    Journal: BMC Plant Biology

    Article Title: Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection

    doi: 10.1186/s12870-018-1391-5

    Figure Lengend Snippet: Ectopic expression of OsWRKY42 suppresses salt and cellulase induced expression of JA biosynthesis and responsive genes in Arabidopsis transgenic lines. Expression of JA biosynthesis and response genes was measured in 35S::OsWRKY42 transgenic lines and wild type (Col-0) Arabidopsis plants after salt and cellulase treatment. a. For salt treatment, one-week old seedlings (wild type or 35S::OsWRKY42) were treated for 12 h with either MS or MS with 150 mM NaCl. b. For cellulase treatment, leaves of three weeks old Arabidopsis plants (wild type or 35S::OsWRKY42) were infiltrated with 2 U/ml of cellulase (Sigma) . Leaves were harvested after 12 h and processed for qPCR analysis. The graph represents the relative fold change using expression values from wild type or 35S::OsWRKY42 lines treated with water. c. Leaves of fourteen days old rice seedlings were syringe infiltrated with either LBA4404 or LBA4404/pH7FWG2::OsWRKY42. Twelve hours post treatment, leaves were harvested and processed for qRT-PCR. The relative fold change was calculated over control (leaves infiltrated only with LBA4404). AtUBQ5 and OsGAPDH were used as endogenous controls for rice and Arabidopsis, respectively. The average from three biological replicates is plotted on the graph. The error bar represents standard deviation. Data was analysed using the Student’s t -test for independent means. The asterisk above the bar indicates significant difference with p value

    Article Snippet: The transgenic nature of the T1 plants was further confirmed by direct PCR (Terra PCR direct polymerase kit, Clontech using leaf tissue and sequencing of the amplified product.

    Techniques: Expressing, Transgenic Assay, Mass Spectrometry, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Standard Deviation

    Ectopic expression of OsWRKY42 enhances tolerance to salt stress in Arabidopsis transgenic lines. a. One-week-old TN-1 rice seedlings (n = 10) were dipped in each of the following: 150 mM NaCl solution, 20% ( w / v ) PEG-6000 and mock (water). Leaves were harvested 12 h post treatment and processed for qRT-PCR. Relative fold change was calculated over water treated samples and OsGAPDH was used as an internal control. b. For assaying salt tolerance, one-week-old Arabidopsis seedlings that are either constitutively expressing OsWRKY42 (35S::OsWRKY42) or wild type (Col-0) ( n = 20) were grown on MS agar medium with or without NaCl (150 mM, Merck). (c-d) The total fresh weight and number of root branches of individual seedlings was quantified on the fifteenth day post-treatment. e . For anthocyanin estimation, three weeks old Arabidopsis plants (wild type and 35S::OsWRKY42) were treated with either 150 mM NaCl or water. On the fifteenth day post treatment, leaves ( n = 3) were collected and anthocyanin was extracted in acidic methanol. Anthocyanin was estimated using a spectrophotometer and the anthocyanin content is expressed as absorbance per milligram of fresh weight (F.W). f . ROS estimation was done in detached leaves from adult plants. Leaf discs (n = 15) of either wild type or 35S::OsWRKY42 lines were placed on sterile filter papers soaked in MS solution either with or without NaCl (150 mM). 12 h post treatment, the leaves were stained with NBT. The O 2 − content was estimated spectrophotometrically and is expressed as change in absorbance per milligram of fresh weight. Leaves from three different plants were used as replicates. The data was analysed using one-way ANOVA and the Tukey-Karmer honestly significance difference test. The alphabets above the bar indicates significant difference with p value

    Journal: BMC Plant Biology

    Article Title: Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection

    doi: 10.1186/s12870-018-1391-5

    Figure Lengend Snippet: Ectopic expression of OsWRKY42 enhances tolerance to salt stress in Arabidopsis transgenic lines. a. One-week-old TN-1 rice seedlings (n = 10) were dipped in each of the following: 150 mM NaCl solution, 20% ( w / v ) PEG-6000 and mock (water). Leaves were harvested 12 h post treatment and processed for qRT-PCR. Relative fold change was calculated over water treated samples and OsGAPDH was used as an internal control. b. For assaying salt tolerance, one-week-old Arabidopsis seedlings that are either constitutively expressing OsWRKY42 (35S::OsWRKY42) or wild type (Col-0) ( n = 20) were grown on MS agar medium with or without NaCl (150 mM, Merck). (c-d) The total fresh weight and number of root branches of individual seedlings was quantified on the fifteenth day post-treatment. e . For anthocyanin estimation, three weeks old Arabidopsis plants (wild type and 35S::OsWRKY42) were treated with either 150 mM NaCl or water. On the fifteenth day post treatment, leaves ( n = 3) were collected and anthocyanin was extracted in acidic methanol. Anthocyanin was estimated using a spectrophotometer and the anthocyanin content is expressed as absorbance per milligram of fresh weight (F.W). f . ROS estimation was done in detached leaves from adult plants. Leaf discs (n = 15) of either wild type or 35S::OsWRKY42 lines were placed on sterile filter papers soaked in MS solution either with or without NaCl (150 mM). 12 h post treatment, the leaves were stained with NBT. The O 2 − content was estimated spectrophotometrically and is expressed as change in absorbance per milligram of fresh weight. Leaves from three different plants were used as replicates. The data was analysed using one-way ANOVA and the Tukey-Karmer honestly significance difference test. The alphabets above the bar indicates significant difference with p value

    Article Snippet: The transgenic nature of the T1 plants was further confirmed by direct PCR (Terra PCR direct polymerase kit, Clontech using leaf tissue and sequencing of the amplified product.

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR, Mass Spectrometry, Spectrophotometry, Staining

    Targeted deletion of GH receptor in MΦ . A , genomic DNA PCR for Cre and the floxed GH receptor exon 4; absence of AC band containing exon 4 in peritoneal MΦ with retention of band in liver DNA. B , GHR mRNA expression is extinguished in

    Journal: The Journal of Biological Chemistry

    Article Title: Targeted Deletion of Growth Hormone (GH) Receptor in Macrophage Reveals Novel Osteopontin-mediated Effects of GH on Glucose Homeostasis and Insulin Sensitivity in Diet-induced Obesity *

    doi: 10.1074/jbc.M113.460212

    Figure Lengend Snippet: Targeted deletion of GH receptor in MΦ . A , genomic DNA PCR for Cre and the floxed GH receptor exon 4; absence of AC band containing exon 4 in peritoneal MΦ with retention of band in liver DNA. B , GHR mRNA expression is extinguished in

    Article Snippet: DNA isolation and PCR were carried out using Terra PCR Direct Polymerase Mix (Clonetech).

    Techniques: Polymerase Chain Reaction, Expressing