Journal: Frontiers in Plant Science

Article Title: Establishment of a TRV-VIGS system in Coix ( Coix lacryma-jobi L.) for functional study of host–pathogen interactions

doi: 10.3389/fpls.2026.1816009

Figure Lengend Snippet: Optimization of TRV-mediated VIGS in Coix through Agrobacterium concentration and immunosuppressant treatment. (A) Effects of different Agrobacterium concentrations on survival rate of germination seeds and TRV-VIGS efficiency in Coix. (B) Effects of different immunosuppressants (tenoxicam and methylglyoxal) on TRV-VIGS efficiency in Coix at an Agrobacterium concentration of OD600 = 0.3. Data are presented as mean ± SD (n = 3). Similar results were obtained from three independent biological replicates. Statistical significance was determined using one-way ANOVA with Tukey’s test (Bars with different letters are significantly different at p < 0.05).

Article Snippet: Stock solutions (100 mM) of Tenoxicam (MACKLIN ® , Shanghai Macklin Biochemical Technology Co., Ltd.) and Methylglyoxal (MACKLIN ® , Shanghai Macklin Biochemical Technology Co., Ltd.) were prepared using sterile deionized water.

Techniques: Concentration Assay

Journal: Germs

Article Title: Effect of dexamethasone and tenoxicam on the virulence activities of different Pseudomonas aeruginosa clinical isolates

doi: 10.18683/germs.2023.1401

Figure Lengend Snippet: Different drugs and antimicrobial combinations tested in this study

Article Snippet: The turbidity-adjusted cultures were centrifuged and the supernatant of each bacterial isolate (20 μL) was incubated with skim milk (1.25%, 100 μL) and 1.5 μL of either dexamethasone (final concentration 0.0052 μg) or tenoxicam (final concentration 2.7μg/mL) in Eppendorf tube at 37°C for 15 min.

Techniques: Concentration Assay

Journal: Germs

Article Title: Effect of dexamethasone and tenoxicam on the virulence activities of different Pseudomonas aeruginosa clinical isolates

doi: 10.18683/germs.2023.1401

Figure Lengend Snippet: Impact of dexamethasone or tenoxicam on proteolytic and hemolytic activity

Article Snippet: The turbidity-adjusted cultures were centrifuged and the supernatant of each bacterial isolate (20 μL) was incubated with skim milk (1.25%, 100 μL) and 1.5 μL of either dexamethasone (final concentration 0.0052 μg) or tenoxicam (final concentration 2.7μg/mL) in Eppendorf tube at 37°C for 15 min.

Techniques: Activity Assay, Negative Control, Comparison, Control

Journal: Germs

Article Title: Effect of dexamethasone and tenoxicam on the virulence activities of different Pseudomonas aeruginosa clinical isolates

doi: 10.18683/germs.2023.1401

Figure Lengend Snippet: Cip: ciprofloxacin; dexa: dexamethasone; ten: tenoxicam; +ve: positive.

Article Snippet: The turbidity-adjusted cultures were centrifuged and the supernatant of each bacterial isolate (20 μL) was incubated with skim milk (1.25%, 100 μL) and 1.5 μL of either dexamethasone (final concentration 0.0052 μg) or tenoxicam (final concentration 2.7μg/mL) in Eppendorf tube at 37°C for 15 min.

Techniques:

Journal: Germs

Article Title: Effect of dexamethasone and tenoxicam on the virulence activities of different Pseudomonas aeruginosa clinical isolates

doi: 10.18683/germs.2023.1401

Figure Lengend Snippet: G: gentamicin; dexa: dexamethasone; ten: tenoxicam; +ve: positive.

Article Snippet: The turbidity-adjusted cultures were centrifuged and the supernatant of each bacterial isolate (20 μL) was incubated with skim milk (1.25%, 100 μL) and 1.5 μL of either dexamethasone (final concentration 0.0052 μg) or tenoxicam (final concentration 2.7μg/mL) in Eppendorf tube at 37°C for 15 min.

Techniques: