telomerase activity assays cancer cells  (Roche)


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    Roche telomerase activity assays cancer cells
    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding <t>telomerase</t> activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.
    Telomerase Activity Assays Cancer Cells, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/telomerase activity assays cancer cells/product/Roche
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    telomerase activity assays cancer cells - by Bioz Stars, 2020-09
    92/100 stars

    Related Products / Commonly Used Together

    telotaggg telomerase pcr elisa kit

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    1) Product Images from "Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells"

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    Journal: Oncotarget

    doi: 10.18632/oncotarget.25993

    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.
    Figure Legend Snippet: STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Techniques Used: Binding Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.
    Figure Legend Snippet: Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Techniques Used: Activity Assay

    Related Articles

    Polymerase Chain Reaction:

    Article Title: A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells
    Article Snippet: .. Telomerase activity assays Cancer cells were processed according to the manufacturer’s protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA. .. Briefly, cell pellets were thawed in lysis reagent, incubated on ice for 30 minutes, and centrifuged at 16,000 g for 20 minutes at 4° C. Telomerase activity was immediately measured in the resultant supernatant using the telomeric repeat amplification protocol in which telomerase, if present in the cell lysate, adds telomeric repeats to the 3′ end of a biotin-labeled synthetic P1-TS primer.

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells
    Article Snippet: .. Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA. .. Briefly, cell pellets were thawed in lysis reagent, incubated on ice for 30 minutes, and centrifuged at 16,000 g for 20 minutes at 4°C.

    Enzyme-linked Immunosorbent Assay:

    Article Title: A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells
    Article Snippet: .. Telomerase activity assays Cancer cells were processed according to the manufacturer’s protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA. .. Briefly, cell pellets were thawed in lysis reagent, incubated on ice for 30 minutes, and centrifuged at 16,000 g for 20 minutes at 4° C. Telomerase activity was immediately measured in the resultant supernatant using the telomeric repeat amplification protocol in which telomerase, if present in the cell lysate, adds telomeric repeats to the 3′ end of a biotin-labeled synthetic P1-TS primer.

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells
    Article Snippet: .. Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA. .. Briefly, cell pellets were thawed in lysis reagent, incubated on ice for 30 minutes, and centrifuged at 16,000 g for 20 minutes at 4°C.

    Activity Assay:

    Article Title: A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells
    Article Snippet: .. Telomerase activity assays Cancer cells were processed according to the manufacturer’s protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA. .. Briefly, cell pellets were thawed in lysis reagent, incubated on ice for 30 minutes, and centrifuged at 16,000 g for 20 minutes at 4° C. Telomerase activity was immediately measured in the resultant supernatant using the telomeric repeat amplification protocol in which telomerase, if present in the cell lysate, adds telomeric repeats to the 3′ end of a biotin-labeled synthetic P1-TS primer.

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells
    Article Snippet: .. Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA. .. Briefly, cell pellets were thawed in lysis reagent, incubated on ice for 30 minutes, and centrifuged at 16,000 g for 20 minutes at 4°C.

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    Roche telomerase activity assays cancer cells
    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding <t>telomerase</t> activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.
    Telomerase Activity Assays Cancer Cells, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/telomerase activity assays cancer cells/product/Roche
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    telomerase activity assays cancer cells - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Roche telomerase activity
    Correlation between <t>telomerase</t> activity in non-small cell lung cancer (NSCLC) tumor specimens and in pulmonary parenchyma free from tumor infiltration
    Telomerase Activity, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/telomerase activity/product/Roche
    Average 92 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    telomerase activity - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Binding Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Activity Assay

    Correlation between telomerase activity in non-small cell lung cancer (NSCLC) tumor specimens and in pulmonary parenchyma free from tumor infiltration

    Journal: Kardiochirurgia i Torakochirurgia Polska = Polish Journal of Cardio-Thoracic Surgery

    Article Title: Telomerase activity in non-small cell lung cancer

    doi: 10.5114/kitp.2016.58959

    Figure Lengend Snippet: Correlation between telomerase activity in non-small cell lung cancer (NSCLC) tumor specimens and in pulmonary parenchyma free from tumor infiltration

    Article Snippet: The obtained supernatant was used for determining the telomerase activity with the PCR ELISA PLUS protocol ( www.roche-applied-science.com ).

    Techniques: Activity Assay

    Correlation between telomerase activity in non-small cell lung cancer (NSCLC) tumor specimens and the two-year survival rate of the patients

    Journal: Kardiochirurgia i Torakochirurgia Polska = Polish Journal of Cardio-Thoracic Surgery

    Article Title: Telomerase activity in non-small cell lung cancer

    doi: 10.5114/kitp.2016.58959

    Figure Lengend Snippet: Correlation between telomerase activity in non-small cell lung cancer (NSCLC) tumor specimens and the two-year survival rate of the patients

    Article Snippet: The obtained supernatant was used for determining the telomerase activity with the PCR ELISA PLUS protocol ( www.roche-applied-science.com ).

    Techniques: Activity Assay

    The MCF-7 cells telomerase activity was illustrated. MCF-7 cells were transfected with pshRNA-UBE2D3. After 48 hr, PCR-Elisa assay was used to detect telomerase activity. Furthermore, telomerase activity was also measured by 4 GY X-rays after transfection with pshRNA-UBE2D3 and negative control, which showed that MCF-7 cells treated with X-rays after transfection with pshRNA-UBE2D3 showed higher telomerase activity compared with transfection with pshRNA-UBE2D3 alone. Data represented Mean±SD of three independent experiments performed in triplicate. Error bars represent standard deviations.

    Journal: PLoS ONE

    Article Title: Inhibition of UBE2D3 Expression Attenuates Radiosensitivity of MCF-7 Human Breast Cancer Cells by Increasing hTERT Expression and Activity

    doi: 10.1371/journal.pone.0064660

    Figure Lengend Snippet: The MCF-7 cells telomerase activity was illustrated. MCF-7 cells were transfected with pshRNA-UBE2D3. After 48 hr, PCR-Elisa assay was used to detect telomerase activity. Furthermore, telomerase activity was also measured by 4 GY X-rays after transfection with pshRNA-UBE2D3 and negative control, which showed that MCF-7 cells treated with X-rays after transfection with pshRNA-UBE2D3 showed higher telomerase activity compared with transfection with pshRNA-UBE2D3 alone. Data represented Mean±SD of three independent experiments performed in triplicate. Error bars represent standard deviations.

    Article Snippet: The telomerase activity of each sample was determined using the Telo-TAGGG Telomerase PCR-Elisa Kit (Roche, Switzerland) per the manufacturer’s instructions.

    Techniques: Activity Assay, Transfection, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Negative Control

    Kaplan-Meier plots of Disease Free Survival (DFS) considering telomerase activity. Kaplan-Meier survival curves in relation to telomerase activity. Numbers in brackets represent cases with tumour recurrence and crosses indicate censored data

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Telomere length and telomerase activity in non-small cell lung cancer prognosis: clinical usefulness of a specific telomere status

    doi: 10.1186/s13046-015-0195-9

    Figure Lengend Snippet: Kaplan-Meier plots of Disease Free Survival (DFS) considering telomerase activity. Kaplan-Meier survival curves in relation to telomerase activity. Numbers in brackets represent cases with tumour recurrence and crosses indicate censored data

    Article Snippet: Telomerase activity determination Telomerase activity was measured using the Telomeric Repeat Amplification protocol (TRAP)-based telomerase polymerase chain reaction (PCR)-enzyme-linked immunosorbent assay (ELISA) kit (Roche Applied Science, Germany), as described earlier [ ].

    Techniques: Activity Assay