Structured Review

Heraeus Kulzer technovit 8100
Effects of insulin on  E. multilocularis  larval development. A)  Morphology of primary cell aggregates. Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with or without 10 nM human insulin for one week. The aggregates were fixed and embedded in Technovit 8100. Sections (4 μm) were stained with haematoxylin/eosin.  B)  Formation of metacestode vesicles. Primary cells were cultivated in conditioned medium supplemented with human insulin for three weeks and mature metacestode vesicles were counted. Control was set to 1 and results were normalised against the control. (*)  P  values below 0.05, (**) very significant for  P  between 0.001 and 0.01, (***) extremely significant for  P
Technovit 8100, supplied by Heraeus Kulzer, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/technovit 8100/product/Heraeus Kulzer
Average 91 stars, based on 33 article reviews
Price from $9.99 to $1999.99
technovit 8100 - by Bioz Stars, 2020-08
91/100 stars

Images

1) Product Images from "Host insulin stimulates Echinococcus multilocularis insulin signalling pathways and larval development"

Article Title: Host insulin stimulates Echinococcus multilocularis insulin signalling pathways and larval development

Journal: BMC Biology

doi: 10.1186/1741-7007-12-5

Effects of insulin on  E. multilocularis  larval development. A)  Morphology of primary cell aggregates. Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with or without 10 nM human insulin for one week. The aggregates were fixed and embedded in Technovit 8100. Sections (4 μm) were stained with haematoxylin/eosin.  B)  Formation of metacestode vesicles. Primary cells were cultivated in conditioned medium supplemented with human insulin for three weeks and mature metacestode vesicles were counted. Control was set to 1 and results were normalised against the control. (*)  P  values below 0.05, (**) very significant for  P  between 0.001 and 0.01, (***) extremely significant for  P
Figure Legend Snippet: Effects of insulin on E. multilocularis larval development. A) Morphology of primary cell aggregates. Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with or without 10 nM human insulin for one week. The aggregates were fixed and embedded in Technovit 8100. Sections (4 μm) were stained with haematoxylin/eosin. B) Formation of metacestode vesicles. Primary cells were cultivated in conditioned medium supplemented with human insulin for three weeks and mature metacestode vesicles were counted. Control was set to 1 and results were normalised against the control. (*) P values below 0.05, (**) very significant for P between 0.001 and 0.01, (***) extremely significant for P

Techniques Used: Isolation, Staining

Effects of HNMPA(AM) 3 on parasite larvae. A) Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with 10 nM human insulin, with or without HNMPA(AM) 3 . After three weeks of incubation, mature vesicles were counted. Insulin was added to the cultures in order to obtain vesicle formation within three weeks. B) Formation of primary cell aggregates in the presence of HNMPA(AM) 3. Primary cells were incubated as in (A) for seven days before aggregates were fixed, embedded in Technovit 8100 and 4 μm sections stained with haematoxylin/eosin. Note the profound effect of HNMPA(AM) 3 on parasite aggregates already after seven days. Ctrl, DMSO control. C) Protoscoleces were treated with HNMPA(AM) 3 for two weeks under axenic conditions. Protoscolex viability was analysed by counter-staining with methylene blue. D) Metacestode vesicles were treated for one week with 100 μM HNMPA(AM) 3 under axenic conditions. Survival was assessed by counting physically damaged vesicles. Vesicles were incubated in the presence of conditioned medium for optimal maintenance and survival conditions. (*) P values below 0.05, (**) very significant for P between 0.001 and 0.01, (***) extremely significant for P
Figure Legend Snippet: Effects of HNMPA(AM) 3 on parasite larvae. A) Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with 10 nM human insulin, with or without HNMPA(AM) 3 . After three weeks of incubation, mature vesicles were counted. Insulin was added to the cultures in order to obtain vesicle formation within three weeks. B) Formation of primary cell aggregates in the presence of HNMPA(AM) 3. Primary cells were incubated as in (A) for seven days before aggregates were fixed, embedded in Technovit 8100 and 4 μm sections stained with haematoxylin/eosin. Note the profound effect of HNMPA(AM) 3 on parasite aggregates already after seven days. Ctrl, DMSO control. C) Protoscoleces were treated with HNMPA(AM) 3 for two weeks under axenic conditions. Protoscolex viability was analysed by counter-staining with methylene blue. D) Metacestode vesicles were treated for one week with 100 μM HNMPA(AM) 3 under axenic conditions. Survival was assessed by counting physically damaged vesicles. Vesicles were incubated in the presence of conditioned medium for optimal maintenance and survival conditions. (*) P values below 0.05, (**) very significant for P between 0.001 and 0.01, (***) extremely significant for P

Techniques Used: Isolation, Incubation, Staining

2) Product Images from "Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs"

Article Title: Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs

Journal: bioRxiv

doi: 10.1101/2020.04.03.023978

Characterisation of potential resins and optimisation of embedding procedure. A) Hardness measurements for 3 candidate resins (Technovit 7100, Technovit 8100 and Spurr with Technovit 8100 under two different oxygen exclusion conditions: oil or vacuum. Hardness measurements were made using a Shore Duromenter A at two axial positions: top surface and block centre, and at 3 lateral positions for each axial: centre, mid and edge (as indicated in the diagram. Measurements were made in triplicate on two independent samples for each case (mean and standard deviation shown. Comparing the two graphs show that all samples had greater hardness at the centre of the block than the top of the block and at each axial position there was a gradient of decreasing hardness for Spurr resin from the centre to the edge. Technovit 8100 set under oil had the lowest hardness at the top of the block but in the centre all resins had similar measured hardness. B) Diagram showing the simple procedure for embedding samples in known orientation with Orasol Black whilst ensuring the correct FOV can be set at imaging. C) Hardness measurements for Technovit 8100 set under vacuum with three different amounts of secondary catalyst (0.3, 0.4 or 0.5 mL per 15 mL of infiltration soln.) and Orasol Black (2,4,8 mg/mL). Hardness was measured on the top of the block (axially) and in the centre of the block (laterally) with 2 replicate measures on two independent samples in each case. Results show a significant increase in hardness with increasing secondary catalyst and decreasing concentration of Orasol Black (Two-way anova p=0.0059 for secondary catalyst p=0.0011 for opacifying agent concentration.) D) Representative images of two samples embedded in Technovit 8100 and Technovit 7100, showing the flaky resin/voids that can be encountered more so for Technovit 7100 (scale bar =1mm).
Figure Legend Snippet: Characterisation of potential resins and optimisation of embedding procedure. A) Hardness measurements for 3 candidate resins (Technovit 7100, Technovit 8100 and Spurr with Technovit 8100 under two different oxygen exclusion conditions: oil or vacuum. Hardness measurements were made using a Shore Duromenter A at two axial positions: top surface and block centre, and at 3 lateral positions for each axial: centre, mid and edge (as indicated in the diagram. Measurements were made in triplicate on two independent samples for each case (mean and standard deviation shown. Comparing the two graphs show that all samples had greater hardness at the centre of the block than the top of the block and at each axial position there was a gradient of decreasing hardness for Spurr resin from the centre to the edge. Technovit 8100 set under oil had the lowest hardness at the top of the block but in the centre all resins had similar measured hardness. B) Diagram showing the simple procedure for embedding samples in known orientation with Orasol Black whilst ensuring the correct FOV can be set at imaging. C) Hardness measurements for Technovit 8100 set under vacuum with three different amounts of secondary catalyst (0.3, 0.4 or 0.5 mL per 15 mL of infiltration soln.) and Orasol Black (2,4,8 mg/mL). Hardness was measured on the top of the block (axially) and in the centre of the block (laterally) with 2 replicate measures on two independent samples in each case. Results show a significant increase in hardness with increasing secondary catalyst and decreasing concentration of Orasol Black (Two-way anova p=0.0059 for secondary catalyst p=0.0011 for opacifying agent concentration.) D) Representative images of two samples embedded in Technovit 8100 and Technovit 7100, showing the flaky resin/voids that can be encountered more so for Technovit 7100 (scale bar =1mm).

Techniques Used: Blocking Assay, Standard Deviation, Imaging, Concentration Assay

Related Articles

Electron Microscopy:

Article Title: Aberrant lung remodeling in a mouse model of surfactant dysregulation induced by modulation of the Abca3 gene
Article Snippet: .. After determination of lung volumes (V(lung)) using fluid displacement and measurement of resulting buoyancy forces as previously described , complete lungs were subjected to a systematic uniform random sampling procedure for light and electron microscopy as described elsewhere ( ) and sampled tissue was embedded in Technovit 8100 (Heraeus Kulzer, Wehrheim, Germany) or Epon, respectively. .. For light microscopy, single sections were stained with toluidine blue.

Immunocytochemistry:

Article Title: Pollen development in Annona cherimola Mill. (Annonaceae). Implications for the evolution of aggregated pollen
Article Snippet: .. Immunocytochemistry Immunocytochemistry was performed on Technovit 8100 (Kulzer & Co, Wehrheim, Germany) embedded semithin sections and revealed by fluorochromes, as described previously [ , ]. ..

Transferring:

Article Title: Comparative morphology, biology and histology of reproductive development in three lines of Manihot esculenta Crantz (Euphorbiaceae: Crotonoideae)
Article Snippet: .. The samples were further dehydrated by transferring them to 100 % butanol for 48 h. After impregnation, the samples were embedded in resin, Technovit 8100® (Heraeus Kulzer GmbH, Wehrheim, Germany), and allowed to polymerize for 2 h at room temperature. .. Sections (3.5 μm thick) were obtained using a rotary microtome (Histostat, Reichert Scientific Instruments, NY, USA) and then double-stained with periodic acid Schiff's (Sigma Aldrich, Lyon, France) for starch and protein-specific naphthol blue black.

Sampling:

Article Title: Aberrant lung remodeling in a mouse model of surfactant dysregulation induced by modulation of the Abca3 gene
Article Snippet: .. After determination of lung volumes (V(lung)) using fluid displacement and measurement of resulting buoyancy forces as previously described , complete lungs were subjected to a systematic uniform random sampling procedure for light and electron microscopy as described elsewhere ( ) and sampled tissue was embedded in Technovit 8100 (Heraeus Kulzer, Wehrheim, Germany) or Epon, respectively. .. For light microscopy, single sections were stained with toluidine blue.

other:

Article Title: Asymmetric p38 Activation in Zebrafish
Article Snippet: The processed embryos were refixed in 4% paraformaldehyde, washed in PBS, and then either mounted in 100% glycerol for photography or dehydrated and embedded in Technovit 8100 for sectioning.

Staining:

Article Title: Madagascar ground gecko genome analysis characterizes asymmetric fates of duplicated genes
Article Snippet: .. Plastic sections (10 μm thickness) were made from stained embryos using Technovit 8100 (Heraeus Kulzer) and a Leica RM2125RT microtome. ..

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    Heraeus Kulzer technovit 8100 t8100
    Type VII collagen is visualized within corpora amylacea <t>(T8100).</t> Immuno-electron microscopic image of the inner plexiform layer of a human retina. Immunogold-labeled polyclonal antibody directed against type VII collagen labels two corpora amylacea diffusely. Within the corpora amylacea, there is no evidence of fibrillar collagen or banding. Some background labeling is present. Bar 10 μm.
    Technovit 8100 T8100, supplied by Heraeus Kulzer, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/technovit 8100 t8100/product/Heraeus Kulzer
    Average 88 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    technovit 8100 t8100 - by Bioz Stars, 2020-08
    88/100 stars
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    Type VII collagen is visualized within corpora amylacea (T8100). Immuno-electron microscopic image of the inner plexiform layer of a human retina. Immunogold-labeled polyclonal antibody directed against type VII collagen labels two corpora amylacea diffusely. Within the corpora amylacea, there is no evidence of fibrillar collagen or banding. Some background labeling is present. Bar 10 μm.

    Journal: PLoS ONE

    Article Title: Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers

    doi: 10.1371/journal.pone.0145502

    Figure Lengend Snippet: Type VII collagen is visualized within corpora amylacea (T8100). Immuno-electron microscopic image of the inner plexiform layer of a human retina. Immunogold-labeled polyclonal antibody directed against type VII collagen labels two corpora amylacea diffusely. Within the corpora amylacea, there is no evidence of fibrillar collagen or banding. Some background labeling is present. Bar 10 μm.

    Article Snippet: Specimens were pre-infiltrated, infiltrated, and embedded in Technovit 8100 (T8100) (Heraeus Kulzer, Wehrheim, Germany), according to the instructions of the manufacturer.

    Techniques: Labeling

    Effects of insulin on  E. multilocularis  larval development. A)  Morphology of primary cell aggregates. Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with or without 10 nM human insulin for one week. The aggregates were fixed and embedded in Technovit 8100. Sections (4 μm) were stained with haematoxylin/eosin.  B)  Formation of metacestode vesicles. Primary cells were cultivated in conditioned medium supplemented with human insulin for three weeks and mature metacestode vesicles were counted. Control was set to 1 and results were normalised against the control. (*)  P  values below 0.05, (**) very significant for  P  between 0.001 and 0.01, (***) extremely significant for  P

    Journal: BMC Biology

    Article Title: Host insulin stimulates Echinococcus multilocularis insulin signalling pathways and larval development

    doi: 10.1186/1741-7007-12-5

    Figure Lengend Snippet: Effects of insulin on E. multilocularis larval development. A) Morphology of primary cell aggregates. Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with or without 10 nM human insulin for one week. The aggregates were fixed and embedded in Technovit 8100. Sections (4 μm) were stained with haematoxylin/eosin. B) Formation of metacestode vesicles. Primary cells were cultivated in conditioned medium supplemented with human insulin for three weeks and mature metacestode vesicles were counted. Control was set to 1 and results were normalised against the control. (*) P values below 0.05, (**) very significant for P between 0.001 and 0.01, (***) extremely significant for P

    Article Snippet: For immune-histochemistry using the anti-EmIR2 antiserum, samples were embedded in Technovit 8100 (Heraeus Kulzer, Wehrheim, Germany) and 4 μm sections were taken on glass slides.

    Techniques: Isolation, Staining

    Effects of HNMPA(AM) 3 on parasite larvae. A) Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with 10 nM human insulin, with or without HNMPA(AM) 3 . After three weeks of incubation, mature vesicles were counted. Insulin was added to the cultures in order to obtain vesicle formation within three weeks. B) Formation of primary cell aggregates in the presence of HNMPA(AM) 3. Primary cells were incubated as in (A) for seven days before aggregates were fixed, embedded in Technovit 8100 and 4 μm sections stained with haematoxylin/eosin. Note the profound effect of HNMPA(AM) 3 on parasite aggregates already after seven days. Ctrl, DMSO control. C) Protoscoleces were treated with HNMPA(AM) 3 for two weeks under axenic conditions. Protoscolex viability was analysed by counter-staining with methylene blue. D) Metacestode vesicles were treated for one week with 100 μM HNMPA(AM) 3 under axenic conditions. Survival was assessed by counting physically damaged vesicles. Vesicles were incubated in the presence of conditioned medium for optimal maintenance and survival conditions. (*) P values below 0.05, (**) very significant for P between 0.001 and 0.01, (***) extremely significant for P

    Journal: BMC Biology

    Article Title: Host insulin stimulates Echinococcus multilocularis insulin signalling pathways and larval development

    doi: 10.1186/1741-7007-12-5

    Figure Lengend Snippet: Effects of HNMPA(AM) 3 on parasite larvae. A) Primary cells were isolated from axenic metacestode vesicles and cultivated in 2% FCS/(D)MEM supplemented with 10 nM human insulin, with or without HNMPA(AM) 3 . After three weeks of incubation, mature vesicles were counted. Insulin was added to the cultures in order to obtain vesicle formation within three weeks. B) Formation of primary cell aggregates in the presence of HNMPA(AM) 3. Primary cells were incubated as in (A) for seven days before aggregates were fixed, embedded in Technovit 8100 and 4 μm sections stained with haematoxylin/eosin. Note the profound effect of HNMPA(AM) 3 on parasite aggregates already after seven days. Ctrl, DMSO control. C) Protoscoleces were treated with HNMPA(AM) 3 for two weeks under axenic conditions. Protoscolex viability was analysed by counter-staining with methylene blue. D) Metacestode vesicles were treated for one week with 100 μM HNMPA(AM) 3 under axenic conditions. Survival was assessed by counting physically damaged vesicles. Vesicles were incubated in the presence of conditioned medium for optimal maintenance and survival conditions. (*) P values below 0.05, (**) very significant for P between 0.001 and 0.01, (***) extremely significant for P

    Article Snippet: For immune-histochemistry using the anti-EmIR2 antiserum, samples were embedded in Technovit 8100 (Heraeus Kulzer, Wehrheim, Germany) and 4 μm sections were taken on glass slides.

    Techniques: Isolation, Incubation, Staining

    Characterisation of potential resins and optimisation of embedding procedure. A) Hardness measurements for 3 candidate resins (Technovit 7100, Technovit 8100 and Spurr with Technovit 8100 under two different oxygen exclusion conditions: oil or vacuum. Hardness measurements were made using a Shore Duromenter A at two axial positions: top surface and block centre, and at 3 lateral positions for each axial: centre, mid and edge (as indicated in the diagram. Measurements were made in triplicate on two independent samples for each case (mean and standard deviation shown. Comparing the two graphs show that all samples had greater hardness at the centre of the block than the top of the block and at each axial position there was a gradient of decreasing hardness for Spurr resin from the centre to the edge. Technovit 8100 set under oil had the lowest hardness at the top of the block but in the centre all resins had similar measured hardness. B) Diagram showing the simple procedure for embedding samples in known orientation with Orasol Black whilst ensuring the correct FOV can be set at imaging. C) Hardness measurements for Technovit 8100 set under vacuum with three different amounts of secondary catalyst (0.3, 0.4 or 0.5 mL per 15 mL of infiltration soln.) and Orasol Black (2,4,8 mg/mL). Hardness was measured on the top of the block (axially) and in the centre of the block (laterally) with 2 replicate measures on two independent samples in each case. Results show a significant increase in hardness with increasing secondary catalyst and decreasing concentration of Orasol Black (Two-way anova p=0.0059 for secondary catalyst p=0.0011 for opacifying agent concentration.) D) Representative images of two samples embedded in Technovit 8100 and Technovit 7100, showing the flaky resin/voids that can be encountered more so for Technovit 7100 (scale bar =1mm).

    Journal: bioRxiv

    Article Title: Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs

    doi: 10.1101/2020.04.03.023978

    Figure Lengend Snippet: Characterisation of potential resins and optimisation of embedding procedure. A) Hardness measurements for 3 candidate resins (Technovit 7100, Technovit 8100 and Spurr with Technovit 8100 under two different oxygen exclusion conditions: oil or vacuum. Hardness measurements were made using a Shore Duromenter A at two axial positions: top surface and block centre, and at 3 lateral positions for each axial: centre, mid and edge (as indicated in the diagram. Measurements were made in triplicate on two independent samples for each case (mean and standard deviation shown. Comparing the two graphs show that all samples had greater hardness at the centre of the block than the top of the block and at each axial position there was a gradient of decreasing hardness for Spurr resin from the centre to the edge. Technovit 8100 set under oil had the lowest hardness at the top of the block but in the centre all resins had similar measured hardness. B) Diagram showing the simple procedure for embedding samples in known orientation with Orasol Black whilst ensuring the correct FOV can be set at imaging. C) Hardness measurements for Technovit 8100 set under vacuum with three different amounts of secondary catalyst (0.3, 0.4 or 0.5 mL per 15 mL of infiltration soln.) and Orasol Black (2,4,8 mg/mL). Hardness was measured on the top of the block (axially) and in the centre of the block (laterally) with 2 replicate measures on two independent samples in each case. Results show a significant increase in hardness with increasing secondary catalyst and decreasing concentration of Orasol Black (Two-way anova p=0.0059 for secondary catalyst p=0.0011 for opacifying agent concentration.) D) Representative images of two samples embedded in Technovit 8100 and Technovit 7100, showing the flaky resin/voids that can be encountered more so for Technovit 7100 (scale bar =1mm).

    Article Snippet: Resins used were Technovit 7100 (Heraeus Kulzer, Germany), Technovit 8100 (Heraeus Kulzer, Germany), Spurr resin (Polysciences Inc, USA), LR White (Sigma-Aldrich, USA) and Lowicryl HM20 (Polysciences Inc, USA).

    Techniques: Blocking Assay, Standard Deviation, Imaging, Concentration Assay