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Addgene inc tdp 43 5fl
RNA binding ability of <t>TDP-43</t> is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 <t>5FL</t> (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p
Tdp 43 5fl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92/100 stars

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1) Product Images from "TDP-43 misexpression causes defects in dendritic growth"

Article Title: TDP-43 misexpression causes defects in dendritic growth

Journal: Scientific Reports

doi: 10.1038/s41598-017-15914-4

RNA binding ability of TDP-43 is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 5FL (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p
Figure Legend Snippet: RNA binding ability of TDP-43 is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 5FL (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p

Techniques Used: RNA Binding Assay, Over Expression

Related Articles

Clone Assay:

Article Title: TDP-43 misexpression causes defects in dendritic growth
Article Snippet: Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530). .. Full-length TDP-43, FUS, and TDP-43 5FL were cloned into pCMV-myc vector backbone using Gibson Assembly (NEB).

Mutagenesis:

Article Title: TDP-43 misexpression causes defects in dendritic growth
Article Snippet: Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530). .. Constructs containing various mutations of TDP-43 were generated from pCMV-myc hTDP-43 plasmid by site-directed mutagenesis using QuickChange Site Directed Mutagenesis Kit (Stratagene, San Diego CA).

Construct:

Article Title: TDP-43 misexpression causes defects in dendritic growth
Article Snippet: Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530). .. Constructs containing various mutations of TDP-43 were generated from pCMV-myc hTDP-43 plasmid by site-directed mutagenesis using QuickChange Site Directed Mutagenesis Kit (Stratagene, San Diego CA).

Generated:

Article Title: TDP-43 misexpression causes defects in dendritic growth
Article Snippet: Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530). .. Constructs containing various mutations of TDP-43 were generated from pCMV-myc hTDP-43 plasmid by site-directed mutagenesis using QuickChange Site Directed Mutagenesis Kit (Stratagene, San Diego CA).

Expressing:

Article Title: TDP-43 misexpression causes defects in dendritic growth
Article Snippet: .. Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530). .. Full-length TDP-43, FUS, and TDP-43 5FL were cloned into pCMV-myc vector backbone using Gibson Assembly (NEB).

Plasmid Preparation:

Article Title: TDP-43 misexpression causes defects in dendritic growth
Article Snippet: .. Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530). .. Full-length TDP-43, FUS, and TDP-43 5FL were cloned into pCMV-myc vector backbone using Gibson Assembly (NEB).

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    Addgene inc tdp 43 5fl
    RNA binding ability of <t>TDP-43</t> is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 <t>5FL</t> (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p
    Tdp 43 5fl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tdp 43 5fl/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tdp 43 5fl - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    RNA binding ability of TDP-43 is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 5FL (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p

    Journal: Scientific Reports

    Article Title: TDP-43 misexpression causes defects in dendritic growth

    doi: 10.1038/s41598-017-15914-4

    Figure Lengend Snippet: RNA binding ability of TDP-43 is important for regulating dendritic branching ( a ) (top) Schematic of TDP-43 protein demonstrating the C-terminal fragment (CTF, amino acid residues 170–414) being overexpressed. RRM2: RNA Recognition Motif, NES: Nuclear Export Signal, GRD: Glycine Rich Domain. (bottom) Sholl analysis for indicated conditions (N = 60 for all conditions). The colour of the *represents the experimental condition being tested against control. ( b ) (top) Schematic of TDP-43 protein demonstrating TDP-43 ∆N (amino acid residues 10–414). NLS: Nuclear Localization Signal, RRM1: RNA Recognition Motif. (bottom) Sholl analysis for indicated conditions (Control, N = 57, TDP-43 overexpression, N = 45, TDP-43 ∆N overexpression, N = 54). ( c ) (top) Schematic of TDP-43 protein demonstrating TDP-43 5FL (F147L/F149L/F194L/F229L/F231L). (bottom) Sholl analysis for indicated conditions (Control, N = 87, TDP-43 overexpression, N = 76, TDP-43 5FL overexpression, N = 90). The colour of the *represent the experimental condition being tested against control. ( a–c ) Grey vs pink = not significant. Two-Way ANOVA with Tukey’s test, *p

    Article Snippet: Plasmids expressing full-length human FUS (Addgene plasmid # 29609) and TDP-43 5FL (Addgene plasmid # 84914) was a gift from Aaron Gitler (Stanford University School of Medicine, Stanford CA). pCSCMV:tdTomato was a gift from Gerhart Ryffel (Addgene plasmid # 30530).

    Techniques: RNA Binding Assay, Over Expression