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human c5b  (Hycult Biotech)


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    Hycult Biotech human c5b
    Human C5b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human c5b/product/Hycult Biotech
    Average 94 stars, based on 23 article reviews
    human c5b - by Bioz Stars, 2026-06
    94/100 stars

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    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) <t>C5b-9</t> in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
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    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) <t>C5b-9</t> in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
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    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) C5b-9 in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.

    Journal: medRxiv

    Article Title: Factor H-related protein 3 (FHR-3) deposition in kidney allografts – localization and correlation with complement activation

    doi: 10.1101/2023.10.24.23297478

    Figure Lengend Snippet: Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) C5b-9 in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.

    Article Snippet: Complement deposition was detected using a rabbit polyclonal antibody (pAb) to human C4d (BI-RC4D, Biomedical), a rabbit pAb to human C3d (A0063, Dako), and a FITC-labeled mouse mAb to human C5b-9, clone aE11 (HM2167F, Hycult).

    Techniques: Double Staining, Transplantation Assay, Staining