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Trevigen tbst
Tbst, supplied by Trevigen, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Trevigen
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
92/100 stars

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Incubation:

Article Title: Poly(ADP-Ribose) Polymerase 1 Promotes Oxidative-Stress-Induced Liver Cell Death via Suppressing Farnesoid X Receptor ?
Article Snippet: .. Membranes were then blocked with 5% nonfat milk in TBST (50 mM Tris-HCl [pH 7.6], 150 mM NaCl, 0.2% Tween 20) for 3 h. After incubation with primary antibodies (anti-PARP1 [1:1,000; R & D], anti-PAR [1:1,000; Trevigen], anti-histone H1 [1:200; Santa Cruz], anti-β-actin [1:500; Santa Cruz], anti-GAPDH [1:500; Santa Cruz], and anti-FXR [1:1,000; R & D]) in an antibody diluent (Abcam) at 4°C overnight, membranes were washed 3 times with TBST and were incubated with a peroxidase-conjugated secondary antibody in an antibody diluent at room temperature for 2 h. Specific bands were detected by chemiluminescence assays (ECL detection reagents; Pierce) and were recorded on X-ray film. .. Bio-Rad's Quantity One software (version 4.4) was used for quantification.

Article Title: Poly (ADP-ribose) prevents pathological phase separation of TDP-43 by promoting liquid demixing and stress-granule localization
Article Snippet: .. The membrane was sealed in a hybridization bag (KPL) containing 0.4 ml of TBST containing 50nM PAR polymer (Trevigen) and incubated for 1 hr with rocking and rotation at room temperature. .. The membrane was washed 5 times in TBST (5 mins each) and blocked in TBSMT (5% milk in TBST) for 1 hr then incubated in primary antibody in TBST for 1 hour.

Article Title: Targeting dePARylation selectively suppresses DNA repair–defective and PARP inhibitor–resistant malignancies
Article Snippet: .. After washing with TBST, the membrane was incubated with anti-PAR monoclonal antibody (Trevigen) overnight at 4°C. .. Following standard Western blot method, the signals were visualized by chemiluminescent detection.

Article Title: ADP-ribose polymers localized on Ctcf-Parp1-Dnmt1 complex prevent methylation of Ctcf target sites
Article Snippet: .. After incubation for 1 h at room temperature (21°C), the membranes were extensively washed with TBST and subjected to immunostaining using mouse monoclonal anti-PAR Ab (10 HA, Trevigen) and goat anti-mouse horseradish peroxidase-conjugated Abs (Santa Cruz Biotechnology). ..

Immunostaining:

Article Title: ADP-ribose polymers localized on Ctcf-Parp1-Dnmt1 complex prevent methylation of Ctcf target sites
Article Snippet: .. After incubation for 1 h at room temperature (21°C), the membranes were extensively washed with TBST and subjected to immunostaining using mouse monoclonal anti-PAR Ab (10 HA, Trevigen) and goat anti-mouse horseradish peroxidase-conjugated Abs (Santa Cruz Biotechnology). ..

Hybridization:

Article Title: Poly (ADP-ribose) prevents pathological phase separation of TDP-43 by promoting liquid demixing and stress-granule localization
Article Snippet: .. The membrane was sealed in a hybridization bag (KPL) containing 0.4 ml of TBST containing 50nM PAR polymer (Trevigen) and incubated for 1 hr with rocking and rotation at room temperature. .. The membrane was washed 5 times in TBST (5 mins each) and blocked in TBSMT (5% milk in TBST) for 1 hr then incubated in primary antibody in TBST for 1 hour.

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  • 92
    Trevigen tbst
    Tbst, supplied by Trevigen, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Trevigen
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Trevigen tris buffered saline tween 20 tbst
    Tris Buffered Saline Tween 20 Tbst, supplied by Trevigen, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris buffered saline tween 20 tbst/product/Trevigen
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris buffered saline tween 20 tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Trevigen milk tbst
    Milk Tbst, supplied by Trevigen, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/milk tbst/product/Trevigen
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    milk tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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