Structured Review

SouthernBiotech tbst
Tbst, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/SouthernBiotech
Average 92 stars, based on 117 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
92/100 stars

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Incubation:

Article Title: Serum exosomal-annexin A2 is associated with African-American triple-negative breast cancer and promotes angiogenesis
Article Snippet: .. After washings with TBST, the membrane was incubated with the appropriate secondary antibody (Southern Biotech, AL) at room temperature for 2 h. After washing again with TBST, the membranes were developed using ECL plus (Millipore Corporation, MA) and the image was captured using alpha-imager Fluoretech HD2. .. The following antibodies were used for Western blotting analysis: AnxA2 (BD Biosciences, CA), TSG101 (BD Biosciences, CA), flotillin-1 (BD Biosciences, CA), calnexin (BD Biosciences, CA), GM130 (BD Biosciences, CA), EpCAM (Cell Signaling Technology, MA), and CD9 (Cell Signaling Technology, MA).

Article Title: Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus
Article Snippet: .. Following three 10 min washes in TBST, goat anti-mouse β-gal conjugate diluted 1:1500 in TBST (Southern Biotech, USA) was added to each well and incubated 1 h at room temperature with gentle rocking. .. The cells were washed five times with TBST, and the substrates nitro blue tetrazolium (NBT) and X-gal (5-bromo-4-choloro-3-indolyl-beta-D-galactopyranoside) were added to each well and incubated at 37°C.

Article Title: 0404 inhibits hepatocellular carcinoma through a p53/miR-34a/SIRT1 positive feedback loop
Article Snippet: .. Subsequently, TBST was used to wash the membranes three times and the membranes were incubated at room temperature for 1 h with 1:3000 horseradish peroxidase conjugated anti-rabbit or mouse immunoglobulin G (Southern Biotechnology Associates, USA), followed by three washings in TBST. .. Amersham ECL Plus Western Blotting Detection Reagents (GE Healthcare) were used to visualize the specific bands with a ChemiScope 3300 Mini equipment (CLINX, China).

Article Title: PARP1 depletion improves mitochondrial and heart function in Chagas disease: Effects on POLG dependent mtDNA maintenance
Article Snippet: .. Membranes were washed with TBST and TBS, incubated with HRP-conjugated secondary antibody (1: 10,000 dilution, Southern Biotech, Birmingham AL), and images were acquired by using an Image Quant LAS4000 system (GE Healthcare, Pittsburgh MA). .. Immunoblots were subjected to Ponceau S staining to confirm equal loading and transferring of samples.

Article Title: Maternal Protein Restriction Reduces Expression of Angiotensin I-Converting Enzyme 2 in Rat Placental Labyrinth Zone in Late Pregnancy 1
Article Snippet: .. After blocking in 5% nonfat milk in Tris-buffered saline with Tween-20 (TBST; wt/vol; 20 mM Tris, 150 mM sodium chloride, pH 7.5, 0.05% Tween-20 [vol/vol]), a rabbit anti-ACE polyclonal immunoglobulin G (IgG; Cat. PT344R; Panora Biotech) at 1:1000 dilution in 2% nonfat milk in TBST (wt/vol) or a goat anti-AGTR1 polyclonal IgG (Cat. sc-31181; Santa Cruz) at 1:200 dilution in 2% nonfat milk in TBST was added to nitrocellulose membrane and incubated at room temperature for 2 h. After washing, the blots were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Cat. 1030-06; SouthernBiotech) or HRP-conjugated donkey anti-goat IgG (Cat. 6420-05; SouthernBiotech) at 1:5000 dilution in 2% nonfat milk in TBST at room temperature for 1 h. Proteins in blots were visualized with Pierce enhanced chemiluminescence detection (Cat. 32209; Thermo Scientific) and Blue Lite Autorad film (Cat. F9024; BioExpress) according to the manufacturers' recommendations. .. Beta-actin (ACTB) was used as an internal control for Western blotting in this study.

Article Title: Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis
Article Snippet: .. After being washed five times in TBST, the membranes were then incubated with HRP-conjugated goat anti-chicken IgG (SouthernBiotech,USA) for 1 h at 37°C (diluted 1:6000 in TBST). .. Finally, the immunoreaction was visualized using freshly prepared diaminobenzidine (DAB, Sigma) as a chromogenic substrate after 5 min.

Blocking Assay:

Article Title: Maternal Protein Restriction Reduces Expression of Angiotensin I-Converting Enzyme 2 in Rat Placental Labyrinth Zone in Late Pregnancy 1
Article Snippet: .. After blocking in 5% nonfat milk in Tris-buffered saline with Tween-20 (TBST; wt/vol; 20 mM Tris, 150 mM sodium chloride, pH 7.5, 0.05% Tween-20 [vol/vol]), a rabbit anti-ACE polyclonal immunoglobulin G (IgG; Cat. PT344R; Panora Biotech) at 1:1000 dilution in 2% nonfat milk in TBST (wt/vol) or a goat anti-AGTR1 polyclonal IgG (Cat. sc-31181; Santa Cruz) at 1:200 dilution in 2% nonfat milk in TBST was added to nitrocellulose membrane and incubated at room temperature for 2 h. After washing, the blots were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Cat. 1030-06; SouthernBiotech) or HRP-conjugated donkey anti-goat IgG (Cat. 6420-05; SouthernBiotech) at 1:5000 dilution in 2% nonfat milk in TBST at room temperature for 1 h. Proteins in blots were visualized with Pierce enhanced chemiluminescence detection (Cat. 32209; Thermo Scientific) and Blue Lite Autorad film (Cat. F9024; BioExpress) according to the manufacturers' recommendations. .. Beta-actin (ACTB) was used as an internal control for Western blotting in this study.

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    SouthernBiotech tbst
    Tbst, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/SouthernBiotech
    Average 92 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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