Article Title: Estrogen induces cell proliferation by promoting ABCG2-mediated efflux in endometrial cancer cells
Article Snippet: .. Blotting membranes were blocked by TBST [150 mM NaCl, 0.1% (v/v) Tween20, and 20 mM Tris] containing 5% (v/v) bovine serum albumin (BSA) and reacted with an anti-ABCG2 antibody (1:250, Santa Cruz Biotechnology, YX, USA) or an anti-β-actin antibody (1:1000, Sigma-Aldrich, Tokyo, Japan) followed by Histofine Simple Stain MAX-PO (Nichirei Bioscience, Tokyo, Japan). ..
Article Title: Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3
Article Snippet: .. Following incubation with a horseradish peroxidase (HRP)-labeled secondary antibody against anti-rabbit IgG (LF-SA8002; Ab Frontier, San Diego, CA, USA), anti-goat IgG (cat. no. sc-2020; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-mouse IgG (cat. no. sc-2005; Santa Cruz Biotechnology) was diluted in TBST, the PVDF membrane was reacted with enhanced chemiluminescence reagent (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and then detected using ImageQuant LAS 4000 Mini system (GE Healthcare Life Sciences, Chalfont, UK) according to the manufacturer's protocol. .. Pull-down assay LCH was coupled to CNBr-activated Sepharose™ 4B matrix beads in 0.1 M NaHCO3 (pH 8.3) containing 0.5 M NaCl overnight at 4°C, according to the manufacturer's protocol. Article Title: KAT5 Negatively regulates the proliferation of prostate cancer LNCaP cells via the caspase 3-dependent apoptosis pathway
Article Snippet: .. After incubation with 5% skim milk in TBST (20 mM Tris–HCl (pH 7.6), 137 mM NaCl and 0.1% Tween-20) for 1 h, the membrane was washed 3 times with TBST and incubated with antibodies against PARP-1 (1:1000, sc-56197, Santa Cruz), Caspase 3 (1:1000, #9662, Cell Signaling), Bax (1:500, #2772, Cell Signaling), Cytochrome C (1:1000, sc-13156, Santa Cruz), Bcl-w (1:500, sc-11422, Santa Cruz), Bcl-2 (1:500, sc-7382, Santa Cruz), p21 (1:1000, sc-6246, Santa Cruz), β-actin (1:1000, sc-47778, Santa Cruz), KAT5 (1:1000, sc-5725, Santa Cruz) at room temperature. .. Membranes were washed three times for 10 min with TBST and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated anti-mouse or anti-rabbit antibodies for 1 h. Blots were washed with TBST three times and developed with the Western blotting luminol reagent (sc-2048, Santa Cruz) according to the manufacturer’s protocols. Article Title: Electrical Stimulation with a Conductive Polymer Promotes Neurite Outgrowth and Synaptogenesis in Primary Cortical Neurons in 3D
Article Snippet: .. The membranes were then incubated with synaptophysin, PSD95 (Thermo Fisher Scientific, NSW; dilution factor 1:1000), and PSA-NCAM (Thermo Fisher Scientific, NSW; dilution factor 1:1000) antibodies in TBST containing 1% BSA overnight at 4 o C. Secondary antibodies were anti-rabbit IgG conjugated with horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA; dilution factor 1:5000). .. For visualization, ECL detection reagents were exposed on the GelDoc System (Bio-Rad, Hercules, CA). Article Title: Lipopolysaccharide from Rhodobacter sphaeroides (TLR4 antagonist) attenuates hypersensitivity and modulates nociceptive factors
Article Snippet: .. Proteins were transferred to Immune-Blot PVDF membranes (Bio-Rad, Hercules, CA) using semi-dry transfer (30 min, 25 V), blocked for 1 h at room temperature using 5% non-fat dry milk (Bio-Rad, Hercules, CA) in Tris-buffered saline with 0.1% Tween 20 (TBST), washed in TBST, and then incubated overnight at 4 °C with primary antibodies targeting the following proteins: TLR4 (Santa Cruz Biotechnology, Santa Cruz, CA #sc-293072, 1:500), IBA-1 (Proteintech #10904-1-AP, 1:1000), GFAP (Santa Cruz Biotechnology, Santa Cruz, CA #sc-6171, 1:1000), IL-1β (Abcam, Cambridge, UK #ab9787, 1:1000), IL-1Ra (Abcam, Cambridge, UK #ab175392, 1:1000), IL-18 (Abcam, Cambridge, UK #ab191860, 1:1000), IL-18BP (Novus Biologicals, Cambridge, UK #NB110-57117, 1:1000), IL-6 (Invitrogen, Carlsbad, CA #ARC0062, 1:500), IL-10 (Invitrogen, Carlsbad, CA #ARC17536, 1:1000), MMP-9 (EMD Millipore, Billerica, MA #AB19016, 1:1000), TIMP-1 (Novus Biologicals, Cambridge, UK #NBP1-96554, 1:1000), and GAPDH (EMD Millipore, Billerica, MA #MAB374, 1:5000; as a loading control). .. Blots were incubated with the corresponding HRP-conjugated secondary polyclonal antibody (Vector Laboratories, Burlingame, CA, peroxidase anti-mouse IgG (H + L) #PI-2000; peroxidase anti-mouse IgG (H + L) #PI-1000, 1:5000) for 1 h at RT.
Article Title: Decreased expression of aquaporin 1 correlates with clinicopathological features of patients with cervical cancer
Article Snippet: The membranes were then washed with 1× TBST, and primary antibodies were detected with horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG (Santa Cruz Biotechnology, USA, at a dilution of 1:5,000).
Article Title: Small molecule purine and pseudopurine derivatives: synthesis, cytostatic evaluations and investigation of growth inhibitory effect in non-small cell lung cancer A549
Article Snippet: The next day, membranes were washed in TBST and probed with horseradish peroxidase-conjugated secondary antibodies goat anti-mouse (Santa Cruz Biotechnology) or goat anti-rabbit (Santa Cruz Biotechnology).
Article Title: Role of Hydroxysteroid Dehydrogenase-Like 2 (HSDL2) in Human Ovarian Cancer
Article Snippet: After washing with TBST 3 times, goat anti-mouse IgG coupled to HRP (1: 1000, cat. no. sc-2005, Santa Cruz) as secondary antibody was added and was detected using an EasyBlot ECL kit (cat. no. C506668, Sangon Biotech, Shanghai, China).