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Promega tbst
Tbst, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Promega
Average 92 stars, based on 319 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
92/100 stars

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Concentration Assay:

Article Title: Aberrant Activation of p38 MAP Kinase-Dependent Innate Immune Responses Is Toxic to Caenorhabditis elegans
Article Snippet: .. Laemmli buffer (Bio-Rad Laboratories, Inc.) was added to a concentration of 1X, and the total protein from each sample was resolved on NuPage 4–12% gels (Life Technologies), transferred to nitrocellulose membranes (Life Technologies), blocked with 5% powdered milk in TBST, and probed with a 1:2000 dilution of an antibody that recognizes the doubly phosphorylated TGY motif of PMK-1 (Promega Corporation). .. The blot was then stripped and reprobed with a 1:10,000 dilution of an anti-actin antibody (Thermo Fisher Scientific, Inc.).

Chloramphenicol Acetyltransferase Assay:

Article Title: EWS-FLI1 low Ewing sarcoma cells demonstrate decreased susceptibility to T-cell-mediated tumor cell apoptosis
Article Snippet: .. Following washing (3× for 5 minutes in TBST), secondary antibody anti-Rb-HRP Promega, CATW401B) at a 1:10,000 dilution in TBST was incubated for 1 hour at room temperature. .. Following washes (3× for 5 minutes in TBST), the membrane was incubated in ECL2 (Thermo Scientific, #80196) for 5 min before film development.

Incubation:

Article Title: EWS-FLI1 low Ewing sarcoma cells demonstrate decreased susceptibility to T-cell-mediated tumor cell apoptosis
Article Snippet: .. Following washing (3× for 5 minutes in TBST), secondary antibody anti-Rb-HRP Promega, CAT#W401B) at a 1:10,000 dilution in TBST was incubated for 1 hour at room temperature. .. Following washes (3× for 5 minutes in TBST), the membrane was incubated in ECL2 (Thermo Scientific, #80196) for 5 min before film development.

Article Title: Increased Phosphorylation of Ser-Gln Sites on SUPPRESSOR OF GAMMA RESPONSE1 Strengthens the DNA Damage Response in Arabidopsis thaliana
Article Snippet: .. Because SOG1-Myc can be detected using an anti-Myc antibody, the membrane was incubated for 2 h at room temperature in the anti-Myc primary antibody A-14 (1:2000 dilution, lot no. F0810; Santa Cruz Biotechnology), rinsed three times with 1× TBST (0.01 M Tris-HCl, 0.15 M NaCl, and 0.05% Triton X-100), and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc. .. Next, the membrane was washed three times with 1× TBST and processed with a LAS-4000 luminescent image analyzer (Fujifilm) after incubation with the ECL Prime enhanced chemiluminescence kit (GE Healthcare).

Article Title: Disulfide Bond Formation Contributes to Herpes Simplex Virus Capsid Stability and Retention of Pentons ▿
Article Snippet: .. Proteins were electrotransferred to polyvinylidene difluoride (PVDF) membrane at 35 V overnight and blocked with 5% fat free milk, and membranes were incubated with primary antibody specific for various capsid proteins for 1.5 h. After washing with TBST (150 mM NaCl, 20 mM Tris, pH 7.5, and 0.1% Tween), blots were incubated with alkaline phosphatase-conjugated secondary antibodies at 1:5,000 for 1 h, washed again with TBST, and developed using Promega color detection reagents according to the manufacturer's instructions. ..

Article Title: Evaluating rational non-cross-resistant combination therapy in advanced clear cell renal cell carcinoma: combined mTOR and AKT inhibitor therapy
Article Snippet: .. After washing with TBST, membranes were incubated with a horseradish peroxidase-linked anti-mouse or anti-rabbit secondary antibody (1:5,000 dilution) (Promega, Madison, WI), washed again, and then incubated with enhanced chemiluminescence reagent (Amersham-Pharmacia, Piscata-way, NJ). ..

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  • 92
    Promega tbst
    Tbst, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Promega
    Average 92 stars, based on 319 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Promega tbst buffer
    Tbst Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst buffer/product/Promega
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    tbst buffer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    88
    Promega tbst buffer ph7 5
    Tbst Buffer Ph7 5, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst buffer ph7 5/product/Promega
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tbst buffer ph7 5 - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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