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Millipore tbst
Binding of anti-CD59 antibody (1:300) to paramyosin (Pmy). (Lanes a to c) NPRM from schistosomula or affinity purified protein were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with a 5% milk solution in <t>TBST</t> for 1 h at room temperature, the membrane was treated with rabbit anti-CD59 antibody or normal rabbit serum (NRS) (1:300) and then washed and treated with peroxidase-conjugated goat anti-rabbit <t>IgG</t> as a second antibody (1:5,000). Bands were developed by enhanced chemiluminescence. Lane a, NPRM of schistosomula; lane b, effluent of affinity column; lane c, eluate of affinity column; lane d, SCIP-1 (arrowhead) eluted from DEAE-cellulose (peak tube) was subjected to SDS-PAGE and the gel was stained with Coomassie blue; lane m, stained molecular mass markers (200, 120, 97, and 67 kDa).
Tbst, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 3521 article reviews
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Images

1) Product Images from "Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin "

Article Title: Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin

Journal: Infection and Immunity

doi: 10.1128/IAI.71.11.6402-6410.2003

Binding of anti-CD59 antibody (1:300) to paramyosin (Pmy). (Lanes a to c) NPRM from schistosomula or affinity purified protein were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with a 5% milk solution in TBST for 1 h at room temperature, the membrane was treated with rabbit anti-CD59 antibody or normal rabbit serum (NRS) (1:300) and then washed and treated with peroxidase-conjugated goat anti-rabbit IgG as a second antibody (1:5,000). Bands were developed by enhanced chemiluminescence. Lane a, NPRM of schistosomula; lane b, effluent of affinity column; lane c, eluate of affinity column; lane d, SCIP-1 (arrowhead) eluted from DEAE-cellulose (peak tube) was subjected to SDS-PAGE and the gel was stained with Coomassie blue; lane m, stained molecular mass markers (200, 120, 97, and 67 kDa).
Figure Legend Snippet: Binding of anti-CD59 antibody (1:300) to paramyosin (Pmy). (Lanes a to c) NPRM from schistosomula or affinity purified protein were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with a 5% milk solution in TBST for 1 h at room temperature, the membrane was treated with rabbit anti-CD59 antibody or normal rabbit serum (NRS) (1:300) and then washed and treated with peroxidase-conjugated goat anti-rabbit IgG as a second antibody (1:5,000). Bands were developed by enhanced chemiluminescence. Lane a, NPRM of schistosomula; lane b, effluent of affinity column; lane c, eluate of affinity column; lane d, SCIP-1 (arrowhead) eluted from DEAE-cellulose (peak tube) was subjected to SDS-PAGE and the gel was stained with Coomassie blue; lane m, stained molecular mass markers (200, 120, 97, and 67 kDa).

Techniques Used: Binding Assay, Affinity Purification, Polyacrylamide Gel Electrophoresis, Blocking Assay, Affinity Column, SDS Page, Staining

Binding of paramyosin to C8 and C9. Purified human C8 and C9 and BSA (1 μg each) were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with 5% milk in TBST, the membrane was incubated with recombinant paramyosin (4 μg/ml) in blocking solution for 2 h at 37°C. After two washes with TBST, the membrane was reacted with monoclonal anti-paramyosin antibody (1:4) (Anti-Pmy) or an isotype-matched, unrelated monoclonal antibody (Control) and then incubated with peroxidase-conjugated goat anti-mouse IgG (1:5,000) for 1 h at room temperature. Bands were visualized by enhanced chemiluminescence. Lane a, C8α and C8γ (upper band) and C8β (lower band); lane b, C9; lane c, BSA.
Figure Legend Snippet: Binding of paramyosin to C8 and C9. Purified human C8 and C9 and BSA (1 μg each) were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with 5% milk in TBST, the membrane was incubated with recombinant paramyosin (4 μg/ml) in blocking solution for 2 h at 37°C. After two washes with TBST, the membrane was reacted with monoclonal anti-paramyosin antibody (1:4) (Anti-Pmy) or an isotype-matched, unrelated monoclonal antibody (Control) and then incubated with peroxidase-conjugated goat anti-mouse IgG (1:5,000) for 1 h at room temperature. Bands were visualized by enhanced chemiluminescence. Lane a, C8α and C8γ (upper band) and C8β (lower band); lane b, C9; lane c, BSA.

Techniques Used: Binding Assay, Purification, Polyacrylamide Gel Electrophoresis, Blocking Assay, Incubation, Recombinant

2) Product Images from "Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin "

Article Title: Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin

Journal: Infection and Immunity

doi: 10.1128/IAI.71.11.6402-6410.2003

Binding of anti-CD59 antibody (1:300) to paramyosin (Pmy). (Lanes a to c) NPRM from schistosomula or affinity purified protein were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with a 5% milk solution in TBST for 1 h at room temperature, the membrane was treated with rabbit anti-CD59 antibody or normal rabbit serum (NRS) (1:300) and then washed and treated with peroxidase-conjugated goat anti-rabbit IgG as a second antibody (1:5,000). Bands were developed by enhanced chemiluminescence. Lane a, NPRM of schistosomula; lane b, effluent of affinity column; lane c, eluate of affinity column; lane d, SCIP-1 (arrowhead) eluted from DEAE-cellulose (peak tube) was subjected to SDS-PAGE and the gel was stained with Coomassie blue; lane m, stained molecular mass markers (200, 120, 97, and 67 kDa).
Figure Legend Snippet: Binding of anti-CD59 antibody (1:300) to paramyosin (Pmy). (Lanes a to c) NPRM from schistosomula or affinity purified protein were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with a 5% milk solution in TBST for 1 h at room temperature, the membrane was treated with rabbit anti-CD59 antibody or normal rabbit serum (NRS) (1:300) and then washed and treated with peroxidase-conjugated goat anti-rabbit IgG as a second antibody (1:5,000). Bands were developed by enhanced chemiluminescence. Lane a, NPRM of schistosomula; lane b, effluent of affinity column; lane c, eluate of affinity column; lane d, SCIP-1 (arrowhead) eluted from DEAE-cellulose (peak tube) was subjected to SDS-PAGE and the gel was stained with Coomassie blue; lane m, stained molecular mass markers (200, 120, 97, and 67 kDa).

Techniques Used: Binding Assay, Affinity Purification, Polyacrylamide Gel Electrophoresis, Blocking Assay, Affinity Column, SDS Page, Staining

Binding of paramyosin to C8 and C9. Purified human C8 and C9 and BSA (1 μg each) were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with 5% milk in TBST, the membrane was incubated with recombinant paramyosin (4 μg/ml) in blocking solution for 2 h at 37°C. After two washes with TBST, the membrane was reacted with monoclonal anti-paramyosin antibody (1:4) (Anti-Pmy) or an isotype-matched, unrelated monoclonal antibody (Control) and then incubated with peroxidase-conjugated goat anti-mouse IgG (1:5,000) for 1 h at room temperature. Bands were visualized by enhanced chemiluminescence. Lane a, C8α and C8γ (upper band) and C8β (lower band); lane b, C9; lane c, BSA.
Figure Legend Snippet: Binding of paramyosin to C8 and C9. Purified human C8 and C9 and BSA (1 μg each) were subjected to SDS-8% PAGE and transferred onto a nitrocellulose membrane. After blocking with 5% milk in TBST, the membrane was incubated with recombinant paramyosin (4 μg/ml) in blocking solution for 2 h at 37°C. After two washes with TBST, the membrane was reacted with monoclonal anti-paramyosin antibody (1:4) (Anti-Pmy) or an isotype-matched, unrelated monoclonal antibody (Control) and then incubated with peroxidase-conjugated goat anti-mouse IgG (1:5,000) for 1 h at room temperature. Bands were visualized by enhanced chemiluminescence. Lane a, C8α and C8γ (upper band) and C8β (lower band); lane b, C9; lane c, BSA.

Techniques Used: Binding Assay, Purification, Polyacrylamide Gel Electrophoresis, Blocking Assay, Incubation, Recombinant

Related Articles

Incubation:

Article Title: Loss of NAMPT in aging retinal pigment epithelium reduces NAD+ availability and promotes cellular senescence
Article Snippet: .. Next day, blots were washed with TBST and incubated with horseradish peroxidase conjugated secondary antibody (1:3000; Sigma-Aldrich, USA) for 60 min with gentle shaking at room temperature. .. Blots were then washed (with TBST) and developed with chemiluminescence reagent (Bio-Rad, Hercules, CA) using autoradiography films (Genesee Scientific, San Diego, CA). β-actin (1:3000; Sigma-Aldrich, USA) expression was evaluated to determine equivalent loading.

Article Title: Anti-Irritant and Anti-Inflammatory Effects of DHA Encapsulated in Resveratrol-Based Solid Lipid Nanoparticles in Human Keratinocytes
Article Snippet: .. As a loading control, membranes were incubated in the presence of stripping solution (for 10 mL solution: In total, 10 mL of 10% SDS, 312 µL HCl, and 6.5 µL β-mercaptoethanol) at 50 °C for 30 min, washed in TBST, blocked, and incubated with a β-actin antibody (clone AC40, catalog # A-4700, Sigma-Aldrich) at a 1:1000 dilution. .. After the incubation with a secondary anti-rabbit (for ASC and cleaved caspase-1), anti-rat (NLRP3), or anti-mouse (for β-actin), immunocomplexes were visualized by using the chemiluminescence detection system (GE Healthcare Life Sciences, Pittsburgh, PA, USA) and quantitated through densitometric analysis.

Article Title: Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin
Article Snippet: .. After two washes with TBST, the membrane was reacted with monoclonal anti-paramyosin antibody (1:4 in TBST) for 1 h at room temperature, washed with TBST, and incubated with peroxidase-conjugated goat anti-mouse IgG (Sigma) (1:5,000) for 1 h at room temperature. ..

Article Title: Differential metabolic effects of constant moderate versus high intensity interval training in high‐fat fed mice: possible role of muscle adiponectin. Differential metabolic effects of constant moderate versus high intensity interval training in high‐fat fed mice: possible role of muscle adiponectin
Article Snippet: .. Membranes were washed as before in again in TBST, and then incubated with a secondary antibody labelled with peroxidase (1:10,000, Anti‐rabbit IgG, catalog number S9169; Sigma® ) for 1 h at room temperature. .. Membranes were then washed with TBST and developed with a chemiluminescent substrate (Clarity™ Western ECL substrate, Bio‐Rad® , catalog number 170‐5061) and visualized on a Chemidoc imaging system (Bio‐Rad® ).

Stripping Membranes:

Article Title: Anti-Irritant and Anti-Inflammatory Effects of DHA Encapsulated in Resveratrol-Based Solid Lipid Nanoparticles in Human Keratinocytes
Article Snippet: .. As a loading control, membranes were incubated in the presence of stripping solution (for 10 mL solution: In total, 10 mL of 10% SDS, 312 µL HCl, and 6.5 µL β-mercaptoethanol) at 50 °C for 30 min, washed in TBST, blocked, and incubated with a β-actin antibody (clone AC40, catalog # A-4700, Sigma-Aldrich) at a 1:1000 dilution. .. After the incubation with a secondary anti-rabbit (for ASC and cleaved caspase-1), anti-rat (NLRP3), or anti-mouse (for β-actin), immunocomplexes were visualized by using the chemiluminescence detection system (GE Healthcare Life Sciences, Pittsburgh, PA, USA) and quantitated through densitometric analysis.

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    Millipore tbst
    Tbst, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Millipore
    Average 99 stars, based on 3521 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    99/100 stars
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