Structured Review

Epizyme tbst
Tbst, supplied by Epizyme, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Epizyme
Average 94 stars, based on 6 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
94/100 stars

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Incubation:

Article Title: High-throughput screening for small molecule inhibitors of the type-I interferon signaling pathway
Article Snippet: .. Membrane was blocked by 5% skim milk in TBST (EpiZyme Biotech) for 1 h. Primary antibodies were diluted according to the manual, incubated with membrane for 16 h at 4 °C. ..

Article Title: ATG3, a Target of miR-431-5p, Promotes Proliferation and Invasion of Colon Cancer via Promoting Autophagy
Article Snippet: .. Next, after washing by TBST, subsequently incubated with secondary antibodies, and washing by TBST again, the protein bands were visualized using chemiluminescent HRP substrate (EpiZyme, Shanghai, China) via a FluorChem FC3 system (Proteinsimple, CA, USA). .. Dual Luciferase Reporter Assay Both psiCHECK2-wild-ATG3-3ʹUTR and psiCHECK2-mutant-ATG3-3ʹUTR luciferase report plasmids of ATG3 were constructed, respectively, according to manufacturer’s instruction (Promega Corporation, WI, USA).

Article Title: Escin suppresses HMGB1‐induced overexpression of aquaporin‐1 and increased permeability in endothelial cells
Article Snippet: .. After an intensive wash, the membrane was incubated with horseradish peroxidase‐conjugated secondary antibodies (1 : 500; EarthOx) at room temperature for 1 h. After being washed three times with TBST, the bands were visualized using an Omni‐ECL Femto Light Chemiluminescence Kit (Epizyme) and quantified using GE Amersham Imager 600 (General Electric Company, Bosto, MA, USA), and the expression levels were normalized to β‐actin. .. F‐actin cytoskeleton staining ECs were grown on glass coverslips and then treated with HMGB1 (100 ng·mL−1 ) or HMGB1 (100 ng·mL−1 ) combined with escin (10, 20, or 30 μg·mL−1 ) or control for 24 h. After stimulation, the cells were fixed in 4% formaldehyde (Solarbio) at 4 °C for 15 min and then permeabilized with 0.2% Triton X‐100 (Solarbio) for 5 min and blocked in 5% BSA in PBS, prior to being incubated with TRITC‐phalloidin (1 : 200; Yeasen) at room temperature in the dark for 1 h. Finally, nuclei were stained with DAPI.

Expressing:

Article Title: Escin suppresses HMGB1‐induced overexpression of aquaporin‐1 and increased permeability in endothelial cells
Article Snippet: .. After an intensive wash, the membrane was incubated with horseradish peroxidase‐conjugated secondary antibodies (1 : 500; EarthOx) at room temperature for 1 h. After being washed three times with TBST, the bands were visualized using an Omni‐ECL Femto Light Chemiluminescence Kit (Epizyme) and quantified using GE Amersham Imager 600 (General Electric Company, Bosto, MA, USA), and the expression levels were normalized to β‐actin. .. F‐actin cytoskeleton staining ECs were grown on glass coverslips and then treated with HMGB1 (100 ng·mL−1 ) or HMGB1 (100 ng·mL−1 ) combined with escin (10, 20, or 30 μg·mL−1 ) or control for 24 h. After stimulation, the cells were fixed in 4% formaldehyde (Solarbio) at 4 °C for 15 min and then permeabilized with 0.2% Triton X‐100 (Solarbio) for 5 min and blocked in 5% BSA in PBS, prior to being incubated with TRITC‐phalloidin (1 : 200; Yeasen) at room temperature in the dark for 1 h. Finally, nuclei were stained with DAPI.

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    Epizyme tbst
    Tbst, supplied by Epizyme, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Epizyme
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

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