tbe buffer  (Millipore)


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  • 99
    Name:
    Glycerol
    Description:

    Catalog Number:
    g5150
    Price:
    None
    Applications:
    Used as carbon source for cultures grown in Terrific Broth and for long-term storage of cultures.
    Buy from Supplier


    Structured Review

    Millipore tbe buffer
    Glycerol

    https://www.bioz.com/result/tbe buffer/product/Millipore
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    tbe buffer - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Dual-Site Phosphorylation of the Control of Virulence Regulator Impacts Group A Streptococcal Global Gene Expression and Pathogenesis"

    Article Title: Dual-Site Phosphorylation of the Control of Virulence Regulator Impacts Group A Streptococcal Global Gene Expression and Pathogenesis

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1004088

    CovR-D53A and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% TBE polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.
    Figure Legend Snippet: CovR-D53A and CovR-T65E bind CovR-regulated promoters with similar affinity to unphosphorylated CovR. Binding of recombinant CovR, CovR-D53A, and CovR-T65E proteins to the promoters of ( A ) hasA and (B) prtS . Increasing concentrations of unphosphorylated CovR, CovR-D53A, and CovR-T65E (monomer concentration given in µM) were used as indicated. Samples were incubated for 15 min at 37°C and electrophoresed on a 6% TBE polyacrylamide gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, non-specific DNA, f, free DNA, cI, lower molecular weight complex, cII, higher molecular weight complex. Gels shown are representatives of identical results obtained on two separate occasions.

    Techniques Used: Binding Assay, Recombinant, Concentration Assay, Incubation, Staining, Molecular Weight

    2) Product Images from "Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment"

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment

    Journal: Protein Science : A Publication of the Protein Society

    doi: 10.1002/pro.117

    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after
    Figure Legend Snippet: Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Techniques Used: Purification, Recombinant, Clear Native PAGE

    3) Product Images from "Use of a Phosphorylation Site Mutant To Identify Distinct Modes of Gene Repression by the Control of Virulence Regulator (CovR) in Streptococcus pyogenes"

    Article Title: Use of a Phosphorylation Site Mutant To Identify Distinct Modes of Gene Repression by the Control of Virulence Regulator (CovR) in Streptococcus pyogenes

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00835-16

    CovR-D53E binding to promoter DNA. Binding of recombinant CovR-D53E protein to DNA of the CovR-D53E regulated promoters of covR (A) and sagA (B) and the CovR-D53E-nonregulated promoters prtS (C) and mac-1 (D). Increasing protein concentrations (in micromolar) were used as indicated. Samples were electrophoresed on a 6% TBE-PAA gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, nonspecific DNA; f, free DNA; c, protein-DNA complex. The triangle symbolizes the continuum from lower- to higher-molecular-weight complexes. Gels shown are representatives of identical results obtained on two separate occasions.
    Figure Legend Snippet: CovR-D53E binding to promoter DNA. Binding of recombinant CovR-D53E protein to DNA of the CovR-D53E regulated promoters of covR (A) and sagA (B) and the CovR-D53E-nonregulated promoters prtS (C) and mac-1 (D). Increasing protein concentrations (in micromolar) were used as indicated. Samples were electrophoresed on a 6% TBE-PAA gel for 60 min at 120 V. The gels were stained with ethidium bromide. ns, nonspecific DNA; f, free DNA; c, protein-DNA complex. The triangle symbolizes the continuum from lower- to higher-molecular-weight complexes. Gels shown are representatives of identical results obtained on two separate occasions.

    Techniques Used: Binding Assay, Recombinant, Staining, Molecular Weight

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model
    Article Snippet: .. For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis. .. 2.6 Quantitative real-time RT-PCR Cells were seeded in 24-well plates at 5 × 104 cells/well.

    In Vitro:

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model
    Article Snippet: .. For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis. .. 2.6 Quantitative real-time RT-PCR Cells were seeded in 24-well plates at 5 × 104 cells/well.

    Autoradiography:

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model
    Article Snippet: .. For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis. .. 2.6 Quantitative real-time RT-PCR Cells were seeded in 24-well plates at 5 × 104 cells/well.

    Incubation:

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model
    Article Snippet: .. For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis. .. 2.6 Quantitative real-time RT-PCR Cells were seeded in 24-well plates at 5 × 104 cells/well.

    Article Title: Efficient Single-Strand Break Repair Requires Binding to Both Poly(ADP-Ribose) and DNA by the Central BRCT Domain of XRCC1
    Article Snippet: .. The adsorbed proteins were mock ribosylated in the absence of NAD+ or ribosylated in the presence of the 50 mM NAD+ (Sigma) in PARP1 reaction buffer (50 mM Tris–HCl pH7.5, 0.8 mM MgCl2 , 1% glycerol and 1.5 mM DTT) containing 40 nM single-stranded oligodeoxyribonucleotide (5′-CATATGCCGGAGATCCGCCTCC-3′) and 5 nM human PARP1 (Trevigen) in a final volume of 50 μL at room temp for 30 min. After rinsing (4 × ) with 50 μL of 0.1% Tween 20 in PBS, 50 μL of His-SUMO-XRCC-BRCT1 or its variants (diluted to 25 nM in 20 mM Tris pH7.5, 130 nM NaCl) were added to the adsorbed proteins and incubated on ice for 30 min. .. The wells were then rinsed (4 × ) as above and incubated with 50 μL mouse anti-polyhistidine (His-tag) Mab (Takara Bio, diluted 1:2500 in 20 mM Tris pH7.5, 130 nM NaCl) followed by 50 μL HRP-conjugated mouse anti-mouse IgG (ECL, GE Healthcare, 1: 5000 in dilution buffer) for 30 min each on ice.

    HAT Assay:

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model
    Article Snippet: .. For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis. .. 2.6 Quantitative real-time RT-PCR Cells were seeded in 24-well plates at 5 × 104 cells/well.

    Activity Assay:

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model
    Article Snippet: .. For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis. .. 2.6 Quantitative real-time RT-PCR Cells were seeded in 24-well plates at 5 × 104 cells/well.

    Mass Spectrometry:

    Article Title: Cryopreservation without vitrification suitable for large scale cryopreservation of orchid seeds
    Article Snippet: .. Therefore, the effect of vitrification was tested using Plant Vitrification Solutions PVS2 (30% (v/v) glycerol (Merck, Hertfordshire, UK), 15% (v/v) ethylene glycol (Sigma-Aldrich, Gillingham, UK), 15% (v/v) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Gillingham, UK) and 0.4 M sucrose ½ Murashige and Skoog solution (½ MS) (Duchefa Biochemie, Haarlem, the Netherlands)) (Sakai et al. ) and PVS3 (50% glycerol, 50% sucrose (Sigma-Aldrich, Gillingham, UK)) (Nishizawa et al. ). ..

    SDS Page:

    Article Title: Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model
    Article Snippet: .. For autoradiography-based in vitro HAT activity assays, HeLa cell nuclear extract was incubated with HAT assay buffer (50 mM HEPES, pH 8.0; 10% glycerol; 1 mM DTT; 1 mM phenylmethylsulfonyl fluoride; and 10 mM sodium butyrate), 1 μL [3 H] acetyl-CoA, and 5 μg of biotinylated-H4 peptide (Millipore, Billerica, MA, USA) along with TA at the indicated concentrations at 30 °C for 1 h. The reactions were stopped by adding 5 × sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) sample buffer and samples were separated on 15% SDS–PAGE gels, followed by autoradiographic analysis. .. 2.6 Quantitative real-time RT-PCR Cells were seeded in 24-well plates at 5 × 104 cells/well.

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  • 99
    Millipore tbe buffer
    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× <t>TBE</t> buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At <t>PCNA2;</t> lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after
    Tbe Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbe buffer/product/Millipore
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    tbe buffer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    Millipore tris borate edta buffer solution
    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× <t>TBE</t> buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At <t>PCNA2;</t> lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after
    Tris Borate Edta Buffer Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris borate edta buffer solution/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris borate edta buffer solution - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment

    doi: 10.1002/pro.117

    Figure Lengend Snippet: Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Article Snippet: His At PCNA1, At PCNA1, His At PCNA2, At PCNA2, and the heterotrimer were separated by 4% native PAGE in 1× TBE buffer and electrotransferred onto a PVDF membrane (0.2 μm) (Millipore), as described previously.

    Techniques: Purification, Recombinant, Clear Native PAGE