tbe buffer  (Millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    Tris Borate EDTA buffer
    Description:

    Catalog Number:
    T3913
    Price:
    None
    Applications:
    TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1x TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5x or 10x stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
    Buy from Supplier


    Structured Review

    Millipore tbe buffer
    Tris Borate EDTA buffer

    https://www.bioz.com/result/tbe buffer/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tbe buffer - by Bioz Stars, 2021-06
    98/100 stars

    Images

    1) Product Images from "Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment"

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment

    Journal: Protein Science : A Publication of the Protein Society

    doi: 10.1002/pro.117

    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after
    Figure Legend Snippet: Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Techniques Used: Purification, Recombinant, Clear Native PAGE

    Related Articles

    Protein Concentration:

    Article Title: Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo
    Article Snippet: Western blotting for protein expression Total protein was extracted from the cells using radioimmunoprecipitation assay buffer supplemented with protease (phenylmethylsulfonyl fluoride) and phosphate inhibitors (NaF anf Na3 VO4 ) (Roche Diagnostics) on ice. .. Protein concentration was determined using a Bicinchoninic Acid protein assay kit (Beyotime Institute of Biotechnology), and loading buffer (5X) was added to the proteins, which were boiled for 5 min. Proteins (50 µ g/lane) were fractionated by 12% SDS-PAGE and electrotransferred onto polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo
    Article Snippet: Western blotting for protein expression Total protein was extracted from the cells using radioimmunoprecipitation assay buffer supplemented with protease (phenylmethylsulfonyl fluoride) and phosphate inhibitors (NaF anf Na3 VO4 ) (Roche Diagnostics) on ice. .. Protein concentration was determined using a Bicinchoninic Acid protein assay kit (Beyotime Institute of Biotechnology), and loading buffer (5X) was added to the proteins, which were boiled for 5 min. Proteins (50 µ g/lane) were fractionated by 12% SDS-PAGE and electrotransferred onto polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA). ..

    SDS Page:

    Article Title: Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo
    Article Snippet: Western blotting for protein expression Total protein was extracted from the cells using radioimmunoprecipitation assay buffer supplemented with protease (phenylmethylsulfonyl fluoride) and phosphate inhibitors (NaF anf Na3 VO4 ) (Roche Diagnostics) on ice. .. Protein concentration was determined using a Bicinchoninic Acid protein assay kit (Beyotime Institute of Biotechnology), and loading buffer (5X) was added to the proteins, which were boiled for 5 min. Proteins (50 µ g/lane) were fractionated by 12% SDS-PAGE and electrotransferred onto polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA). ..

    Staining:

    Article Title: Transactivation of the Receptor-tyrosine Kinase Ephrin Receptor A2 Is Required for the Low Molecular Weight Hyaluronan-mediated Angiogenesis That Is implicated in Tumor Progression *
    Article Snippet: To test for endotoxin contamination of HA, a lipopolysaccharides (LPS) bioAssay ELISA kit (USBiological Life Sciences) was used. .. LMW-HA with HA standards (Sigma and Enzo Life Sciences) was run on 4–20% Tris borate-EDTA gels and stained with Stains-All (Sigma) to confirm LMW-HA purity and size. .. Primary human pulmonary microvascular cells were purchased from Lonza and maintained in EBM-2 growth media supplemented with EGM-2-MV Bulletkit and 10% fetal bovine serum.

    Article Title: VELCRO-IP RNA-seq explores ribosome expansion segment function in translation genome-wide
    Article Snippet: The mixture was incubated at 4°C for 2-3 hours under rotation to permit binding of the RNA to the column. .. Input and unbound RNA samples were compared side by side by 4% polyacrylamide (Ambion)/0.5x TBE (Sigma)/urea (Sigma) gel electrophoresis and SYBR Gold (10,000x, Thermo Fisher, Invitrogen, S11494) staining in 0.5x TBE to assess the efficiency of RNA coupling. .. Input and unbound RNA samples were compared side by side by 4% polyacrylamide (Ambion)/0.5x TBE (Sigma)/urea (Sigma) gel electrophoresis and SYBR Gold (10,000x, Thermo Fisher, Invitrogen, S11494) staining in 0.5x TBE to assess the efficiency of RNA coupling.

    Article Title: Transcriptionally competent chromatin assembled with exogenous histones in a yeast whole cell extract
    Article Snippet: Minichromosomes were deproteinized as described and two-dimensional electrophoresis ( ) was performed in a 20 × 20 cm 1% agarose 1× TBE gel. .. The first dimension was run at 60 V for 16 h and then the gel was shifted 90°, equilibrated for 8 h in the dark in 1× TBE containing 4 μM chloroquine (Sigma) from a fresh stock solution (10 mg/ml) and electrophoresed in the second dimension at 60 V for 16 h. Chloroquine was extensively removed from the gel with several changes in distilled water and the gel was stained with ethidium bromide and destained with distilled water. .. The reference topoisomer ladder was prepared by relaxing negative supercoiled pUC18 plasmid with topoisomerase I in the presence of different amounts of ethidium bromide ( ).

    Nucleic Acid Electrophoresis:

    Article Title: VELCRO-IP RNA-seq explores ribosome expansion segment function in translation genome-wide
    Article Snippet: The mixture was incubated at 4°C for 2-3 hours under rotation to permit binding of the RNA to the column. .. Input and unbound RNA samples were compared side by side by 4% polyacrylamide (Ambion)/0.5x TBE (Sigma)/urea (Sigma) gel electrophoresis and SYBR Gold (10,000x, Thermo Fisher, Invitrogen, S11494) staining in 0.5x TBE to assess the efficiency of RNA coupling. .. Input and unbound RNA samples were compared side by side by 4% polyacrylamide (Ambion)/0.5x TBE (Sigma)/urea (Sigma) gel electrophoresis and SYBR Gold (10,000x, Thermo Fisher, Invitrogen, S11494) staining in 0.5x TBE to assess the efficiency of RNA coupling.

    Polymerase Chain Reaction:

    Article Title: Teratogen-induced alterations in microRNA-34, microRNA-125b and microRNA-155 expression: correlation with embryonic p53 genotype and limb phenotype
    Article Snippet: Briefly, DNA was extracted from the amnion and PCR was performed using PCR mix (Promega), DNA, and 3 primers (Sigma): 5'-ACAGCGTGGTGGTACCTTAT-3', 5'-TATACTCAGAGCCGGCCT-3', and 5'-CTATCAGGACATAGCGTTGG-3' [ ] under the following conditions: initial preheating at 94 8C for 3 min followed by 30 cycles of the following three steps: 1) denaturing (94 8C) for 30 s, 2) annealing (55 8C) for 30 s, and 3) extension (72 8C) for 1 min followed by 3 min at 72 8C. .. PCR products were diluted in a DNA-loading buffer and loaded on 1.2% agarose gel diluted in a TBE buffer with ethidium bromide (Sigma). .. Teratological testing The spectrum of external anomalies induced by CP in mice is very wide [ ].

    Agarose Gel Electrophoresis:

    Article Title: Teratogen-induced alterations in microRNA-34, microRNA-125b and microRNA-155 expression: correlation with embryonic p53 genotype and limb phenotype
    Article Snippet: Briefly, DNA was extracted from the amnion and PCR was performed using PCR mix (Promega), DNA, and 3 primers (Sigma): 5'-ACAGCGTGGTGGTACCTTAT-3', 5'-TATACTCAGAGCCGGCCT-3', and 5'-CTATCAGGACATAGCGTTGG-3' [ ] under the following conditions: initial preheating at 94 8C for 3 min followed by 30 cycles of the following three steps: 1) denaturing (94 8C) for 30 s, 2) annealing (55 8C) for 30 s, and 3) extension (72 8C) for 1 min followed by 3 min at 72 8C. .. PCR products were diluted in a DNA-loading buffer and loaded on 1.2% agarose gel diluted in a TBE buffer with ethidium bromide (Sigma). .. Teratological testing The spectrum of external anomalies induced by CP in mice is very wide [ ].

    Clear Native PAGE:

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment
    Article Snippet: They were then fractionated by 4% native PAGE and analyzed by Western blotting or Coomassie Brilliant Blue staining. .. His At PCNA1, At PCNA1, His At PCNA2, At PCNA2, and the heterotrimer were separated by 4% native PAGE in 1× TBE buffer and electrotransferred onto a PVDF membrane (0.2 μm) (Millipore), as described previously. .. After the transfer, the membrane was blocked with PBS containing 10% (w/v) fat-free milk and 0.1% (v/v) Tween 20 for 1 h. The membrane was incubated with a tetra-His horseradish peroxidase antibody conjugate for 1 h at room temperature (Qiagen, dilution 1:1000).

    Protease Inhibitor:

    Article Title: Investigation of age-related changes in LMNA splicing and expression of progerin in human skeletal muscles
    Article Snippet: .. Muscle tissues (10 mg) from 12 normal individuals were cryosectioned and suspended in 200 μl treatment buffer containing 125 mM Tris-HCl (pH 6.8), 15% SDS (w/v), 10% glycerol (v/v), 0.5 mM phenylmethylsulfonyl fluoride and 9 μl protease inhibitor cocktail (Sigma Aldrich). .. After sonication, 6 pulses of 1 sec duration at a setting of 30/100 on the Sonics sonicator (Sonics, Newtown, CT), the homogenates were stored at -80°C.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Millipore tbe buffer
    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× <t>TBE</t> buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At <t>PCNA2;</t> lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after
    Tbe Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbe buffer/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tbe buffer - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment

    doi: 10.1002/pro.117

    Figure Lengend Snippet: Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Article Snippet: His At PCNA1, At PCNA1, His At PCNA2, At PCNA2, and the heterotrimer were separated by 4% native PAGE in 1× TBE buffer and electrotransferred onto a PVDF membrane (0.2 μm) (Millipore), as described previously.

    Techniques: Purification, Recombinant, Clear Native PAGE

    Characterization of LMW-HA and its effects on EC proliferation. Panel A , to evaluate the molecular weight ( MW ) and purity of our LMW-HA preparation, a 4–20% Tris borate-EDTA ( TBE ) gel was run with HA molecular weight standards (Select-HA TM 75–150

    Journal: The Journal of Biological Chemistry

    Article Title: Transactivation of the Receptor-tyrosine Kinase Ephrin Receptor A2 Is Required for the Low Molecular Weight Hyaluronan-mediated Angiogenesis That Is implicated in Tumor Progression *

    doi: 10.1074/jbc.M114.554766

    Figure Lengend Snippet: Characterization of LMW-HA and its effects on EC proliferation. Panel A , to evaluate the molecular weight ( MW ) and purity of our LMW-HA preparation, a 4–20% Tris borate-EDTA ( TBE ) gel was run with HA molecular weight standards (Select-HA TM 75–150

    Article Snippet: LMW-HA with HA standards (Sigma and Enzo Life Sciences) was run on 4–20% Tris borate-EDTA gels and stained with Stains-All (Sigma) to confirm LMW-HA purity and size.

    Techniques: Molecular Weight