tb green premix ex taq  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    TB Green Premix Ex Taq
    Description:

    Catalog Number:
    RR420A
    Price:
    None
    Category:
    qPCR
    Size:
    200 Rxns
    Buy from Supplier


    Structured Review

    TaKaRa tb green premix ex taq

    https://www.bioz.com/result/tb green premix ex taq/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tb green premix ex taq - by Bioz Stars, 2021-05
    99/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Sendai Virus V Protein Inhibits the Secretion of Interleukin-1β by Preventing NLRP3 Inflammasome Assembly
    Article Snippet: Total RNA was isolated from THP1 macrophages using Nucleospin RNA Plus (TaKaRa, Shiga, Japan), and cDNA was synthesized by reverse transcriptase (Superscript III; Invitrogen, Carlsbad, CA). .. The level of mRNA expression was determined using TB Green Premix EX Taq, ROX plus (TaKaRa). with a StepOne real-time PCR system (Applied Biosystems, Foster City, CA). .. Real-time PCR primers used in this study were as previously described ( ).

    Article Title: SHCBP1 Promotes the Progression of Esophageal Squamous Cell Carcinoma Via the TGFβ Pathway
    Article Snippet: .. Quantitative PCR was conducted by the use of SYBR premix Ex Taq II kit (RR420A; Takara, Japan), and the mRNA expression of SHCBP1 was normalized to the mRNA of glyceraldehyde 3-phosphate dehydrogenase. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Sendai Virus V Protein Inhibits the Secretion of Interleukin-1β by Preventing NLRP3 Inflammasome Assembly
    Article Snippet: Total RNA was isolated from THP1 macrophages using Nucleospin RNA Plus (TaKaRa, Shiga, Japan), and cDNA was synthesized by reverse transcriptase (Superscript III; Invitrogen, Carlsbad, CA). .. The level of mRNA expression was determined using TB Green Premix EX Taq, ROX plus (TaKaRa). with a StepOne real-time PCR system (Applied Biosystems, Foster City, CA). .. Real-time PCR primers used in this study were as previously described ( ).

    Article Title: Overaccumulation of Fat Caused Rapid Reproductive Senescence but not Loss of Ovarian Reserve in ob/ob Mice
    Article Snippet: .. Real-time PCR was performed using SYBR Premix Ex Taq (TaKaRa, Cat #: RR420A, Japan) and a Thermal Cycler Dice Real-Time System TP800 (TaKaRa, Cat #: TP800, Japan). .. Dissociation curves were plotted for all reactions to confirm amplification of a single product with the appropriate melting temperature.

    Article Title: SHCBP1 Promotes the Progression of Esophageal Squamous Cell Carcinoma Via the TGFβ Pathway
    Article Snippet: .. Quantitative PCR was conducted by the use of SYBR premix Ex Taq II kit (RR420A; Takara, Japan), and the mRNA expression of SHCBP1 was normalized to the mRNA of glyceraldehyde 3-phosphate dehydrogenase. ..

    Polymerase Chain Reaction:

    Article Title: Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR
    Article Snippet: A punch of filter paper (2 mm in diameter) was placed in 40 μl SYBR green reaction mixture containing 200 nM of each primer. .. Three different PCR master mix were tested: SYBR green PCR master mix (Diatheva srl, Fano, Italy), RT2 SYBR Green ROX FAST Mastermix (Qiagen, Hilden, Germany), TB Green premix ex Taq II Mastermix (Takara Bio Europe, France). .. Tubes were placed in a thermal cycler (GeneAmp PCR System 2700), and pre-amplified under the following conditions: 94 °C for 5 min, 10 cycles at 94 °C for 30 s, 60 °C for 20 s and 72 °C for 20 s. At the end of this pre-amplification step, the tubes were centrifuged for few seconds and placed in ice; the filter paper was removed and the reaction was split into two PCR tubes (20 μl each tube).

    SYBR Green Assay:

    Article Title: Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR
    Article Snippet: A punch of filter paper (2 mm in diameter) was placed in 40 μl SYBR green reaction mixture containing 200 nM of each primer. .. Three different PCR master mix were tested: SYBR green PCR master mix (Diatheva srl, Fano, Italy), RT2 SYBR Green ROX FAST Mastermix (Qiagen, Hilden, Germany), TB Green premix ex Taq II Mastermix (Takara Bio Europe, France). .. Tubes were placed in a thermal cycler (GeneAmp PCR System 2700), and pre-amplified under the following conditions: 94 °C for 5 min, 10 cycles at 94 °C for 30 s, 60 °C for 20 s and 72 °C for 20 s. At the end of this pre-amplification step, the tubes were centrifuged for few seconds and placed in ice; the filter paper was removed and the reaction was split into two PCR tubes (20 μl each tube).

    Quantitative RT-PCR:

    Article Title: Induction of the cydAB Operon Encoding the bd Quinol Oxidase Under Respiration-Inhibitory Conditions by the Major cAMP Receptor Protein MSMEG_6189 in Mycobacterium smegmatis
    Article Snippet: The contamination of DNA in the isolated RNA was checked by PCR with the primers to be used in quantitative real-time PCR (qRT-PCR). .. To determine the transcript levels of cydA, crp2, MSMEG_3680 , and sigA , qRT-PCR was performed in a 20-μl mixture containing 5 μl of the template cDNA, 15 pmol of each of two gene-specific primers, 10 μl of TB GreenTM Premix Ex TaqTM (Tli RNase Plus) (Takara, Tokyo, Japan), 0.4 μl of the ROX passive fluorescent dye, and 2.6 μl of distilled water. .. Thermal cycling was initiated with 1 cycle at 95°C for 2 min, followed by 40 cycles of 95°C for 5 s and 64°C for 30 s. The sigA gene encoding the principal sigma factor was used as a reference gene for qRT-PCR to normalize the expression levels of cydA, crp2 , and MSMEG_3680 since our RNA sequencing analyses revealed that the sigA gene is constitutively expressed at similar levels in the wild-type (WT), Δaa 3 , Δcrp1 , and Δcrp2 mutant strains ( ).

    Article Title: Srlp is crucial for the self-renewal and differentiation of germline stem cells via RpL6 signals in Drosophila testes
    Article Snippet: Quantitative reverse transcription-PCR Total RNA was extracted using Trizol reagent (9108, Takara, Japan). .. Complementary DNA was synthesized using Prime Script RT Reagent Kit (RR037A, Taraka, Japan), and qRT-PCR was performed using SYBR Premix Ex Taq (RR420A, Takara, Japan). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was amplified as an internal standard.

    Synthesized:

    Article Title: Srlp is crucial for the self-renewal and differentiation of germline stem cells via RpL6 signals in Drosophila testes
    Article Snippet: Quantitative reverse transcription-PCR Total RNA was extracted using Trizol reagent (9108, Takara, Japan). .. Complementary DNA was synthesized using Prime Script RT Reagent Kit (RR037A, Taraka, Japan), and qRT-PCR was performed using SYBR Premix Ex Taq (RR420A, Takara, Japan). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was amplified as an internal standard.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    TaKaRa terra qpcr direct tb green premix
    KIF18A expression was effectively blocked in both A549 and H1975 human lung adenocarcinoma cells caused by its shRNA plasmids. (a) Quantitative <t>PCR</t> assays revealed the dramatically reduced expression levels of KIF18A caused by its shRNA in both A549 and H1975 cells, respectively. (b) Immunoblot assays confirmed the efficient silencing of KIF18A caused by its shRNA plasmids in A549 and H1975 cells. Results are presented as the mean ± SD; ∗ P
    Terra Qpcr Direct Tb Green Premix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/terra qpcr direct tb green premix/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    terra qpcr direct tb green premix - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    KIF18A expression was effectively blocked in both A549 and H1975 human lung adenocarcinoma cells caused by its shRNA plasmids. (a) Quantitative PCR assays revealed the dramatically reduced expression levels of KIF18A caused by its shRNA in both A549 and H1975 cells, respectively. (b) Immunoblot assays confirmed the efficient silencing of KIF18A caused by its shRNA plasmids in A549 and H1975 cells. Results are presented as the mean ± SD; ∗ P

    Journal: Disease Markers

    Article Title: Kinesin Family Member 18A (KIF18A) Contributes to the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells In Vitro and In Vivo

    doi: 10.1155/2019/6383685

    Figure Lengend Snippet: KIF18A expression was effectively blocked in both A549 and H1975 human lung adenocarcinoma cells caused by its shRNA plasmids. (a) Quantitative PCR assays revealed the dramatically reduced expression levels of KIF18A caused by its shRNA in both A549 and H1975 cells, respectively. (b) Immunoblot assays confirmed the efficient silencing of KIF18A caused by its shRNA plasmids in A549 and H1975 cells. Results are presented as the mean ± SD; ∗ P

    Article Snippet: Quantitative PCR was performed using SYBR Ex Taq kit (#638319, Takara, Japan), and the expression levels of KIF18A were normalized to the expression of GAPDH.

    Techniques: Expressing, shRNA, Real-time Polymerase Chain Reaction

    The expression levels of LAPTM4B were obviously decreased in both MG‐63 and U‐2 OS cells after the transfection of LAPTM4B‐targeted shRNA plasmids. (A) The results of quantitative PCR assays showed the obviously reduced expression levels of LAPTM4B in its short hairpin RNA (shRNA) plasmid‐transfected MG‐63 and U‐2 OS cells, respectively. (B) Immunoblot assays revealed the efficient decrease of LAPTM4B expression levels after the transfection of its shRNA plasmids in both MG‐63 and U‐2 OS cells. Results are presented as mean ± SD, * P

    Journal: Orthopaedic Surgery

    Article Title: Identification of Lysosome‐Associated Protein Transmembrane‐4 as a Novel Therapeutic Target for Osteosarcoma Treatment

    doi: 10.1111/os.12692

    Figure Lengend Snippet: The expression levels of LAPTM4B were obviously decreased in both MG‐63 and U‐2 OS cells after the transfection of LAPTM4B‐targeted shRNA plasmids. (A) The results of quantitative PCR assays showed the obviously reduced expression levels of LAPTM4B in its short hairpin RNA (shRNA) plasmid‐transfected MG‐63 and U‐2 OS cells, respectively. (B) Immunoblot assays revealed the efficient decrease of LAPTM4B expression levels after the transfection of its shRNA plasmids in both MG‐63 and U‐2 OS cells. Results are presented as mean ± SD, * P

    Article Snippet: Quantitative PCR was performed using a SYBR Ex Taq kit (638319, Takara, Japan), and the expression levels of LAPTM4B were normalized to the expression of GAPDH.

    Techniques: Expressing, Transfection, shRNA, Real-time Polymerase Chain Reaction, Plasmid Preparation

    AK4 expression levels were markedly decreased in both MCF7 and MDA-MB-231 cells caused by its shRNA transfection. (a) Quantitative PCR assays showed the dramatically reduced expression levels of AK4 after the transfection of its shRNA in MCF7 and MDA-MB-231 cells, respectively. (b) Immunoblot assays showed the efficient decrease of AK4 expression caused by the transfection of its shRNA plasmids in both MCF7 and MDA-MB-231 cells. Results are presented as mean ± SD, ∗ P

    Journal: Disease Markers

    Article Title: AK4 Promotes the Progression of HER2-Positive Breast Cancer by Facilitating Cell Proliferation and Invasion

    doi: 10.1155/2019/8186091

    Figure Lengend Snippet: AK4 expression levels were markedly decreased in both MCF7 and MDA-MB-231 cells caused by its shRNA transfection. (a) Quantitative PCR assays showed the dramatically reduced expression levels of AK4 after the transfection of its shRNA in MCF7 and MDA-MB-231 cells, respectively. (b) Immunoblot assays showed the efficient decrease of AK4 expression caused by the transfection of its shRNA plasmids in both MCF7 and MDA-MB-231 cells. Results are presented as mean ± SD, ∗ P

    Article Snippet: Quantitative PCR was performed using the SYBR Ex Taq kit (638319, Takara, Japan), and the expression levels of AK4 were normalized to the expression of β -actin.

    Techniques: Expressing, Multiple Displacement Amplification, shRNA, Transfection, Real-time Polymerase Chain Reaction

    MACC1 is highly expressed in human osteosarcoma (OS) tissues and cell lines and its expression correlates with prognosis in OS patients. (A) Quantitative PCR assays were conducted to measure the MACC1 mRNA levels in OS tissues and the corresponding adjacent noncancerous tissues ( n = 30). (B) Immunoblot assays were conducted to detect the protein levels of MACC1 in OS tumor tissues and the corresponding adjacent noncancerous tissues. (C) Immunoblot assays were conducted to measure the protein levels of MACC1 in the U-2OS, MG-63, Saos-2, and HOS osteosarcoma cell lines, and normal human umbilical vein endothelial cells (HUVECs). (D) Immunohistochemistry was performed to measure the protein levels of MACC1 in human OS tissues and the corresponding adjacent noncancerous tissues; representative images are shown (×100 and ×400 magnification, respectively). (E) Kaplan–Meyer survival analysis showed the correlation between MACC1 expression in tumor tissues and overall survival or disease-free survival rates.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: MACC1 Contributes to the Development of Osteosarcoma Through Regulation of the HGF/c-Met Pathway and Microtubule Stability

    doi: 10.3389/fcell.2020.00825

    Figure Lengend Snippet: MACC1 is highly expressed in human osteosarcoma (OS) tissues and cell lines and its expression correlates with prognosis in OS patients. (A) Quantitative PCR assays were conducted to measure the MACC1 mRNA levels in OS tissues and the corresponding adjacent noncancerous tissues ( n = 30). (B) Immunoblot assays were conducted to detect the protein levels of MACC1 in OS tumor tissues and the corresponding adjacent noncancerous tissues. (C) Immunoblot assays were conducted to measure the protein levels of MACC1 in the U-2OS, MG-63, Saos-2, and HOS osteosarcoma cell lines, and normal human umbilical vein endothelial cells (HUVECs). (D) Immunohistochemistry was performed to measure the protein levels of MACC1 in human OS tissues and the corresponding adjacent noncancerous tissues; representative images are shown (×100 and ×400 magnification, respectively). (E) Kaplan–Meyer survival analysis showed the correlation between MACC1 expression in tumor tissues and overall survival or disease-free survival rates.

    Article Snippet: Quantitative PCR was performed using the SYBR Ex Taq kit (638319, Takara Bio, Inc.) on a 7900HT Fast Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunohistochemistry