tb green premix ex taq ii mastermix  (TaKaRa)

 
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    Name:
    TB Green Premix Ex Taq
    Description:
    TB Green Premix Ex Taq Tli RNase H Plus an intercalating dye based qPCR master mix for real time RT PCR qPCR is recommended for most real time PCR applications including gene expression analyses genotyping and high throughput studies TB Green Premix Ex Taq Tli RNase H Plus includes TB Green a qPCR reagent for intercalator based RT PCR qPCR In addition to being ideal for most standard real time PCR reactions this kit is recommended when a higher yield of PCR product is needed or when amplifying longer products up to 570 bp This TB Green qPCR premix is supplied at a 2X concentration and allows easy reaction assembly by simply adding primers template and sterile distilled water intercalator based qPCR can be performed
    Catalog Number:
    rr420b
    Price:
    None
    Size:
    400 Rxns
    Category:
    TB Green Premix Ex Taq Tli RNase H Plus qPCR with TB Green detection Real time PCR kits Real time PCR
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    Structured Review

    TaKaRa tb green premix ex taq ii mastermix
    Specificity of qPCR-ML (A) and qPCR-ama (B). The qPCR amplicons were run on a 2% agarose gel. Both qPCR assays were performed using three different PCR master mix: SYBR green PCR master mix, Diatheva (I); RT2 SYBR Green ROX FAST <t>Mastermix,</t> Qiagen (II); TB Green premix ex <t>Taq</t> II Mastermix, Takara Bio (III). For each master mix, DNA from L. ( L. ) infantum (0,006 ng/μl) , L. ( L. ) amazonensis (0,15 ng/μl) , Trypanosoma cruzi (0,1 ng/μl) and human DNA (30 ng/μl) were tested with the condition described in the manuscript. As negative control, a no template control was used for each qPCR run. M: ΦX174 DNA/ Bsu RI ( Hae III) Marker, 9 (ThermoFisher Scientific); NTC: no template control.
    TB Green Premix Ex Taq Tli RNase H Plus an intercalating dye based qPCR master mix for real time RT PCR qPCR is recommended for most real time PCR applications including gene expression analyses genotyping and high throughput studies TB Green Premix Ex Taq Tli RNase H Plus includes TB Green a qPCR reagent for intercalator based RT PCR qPCR In addition to being ideal for most standard real time PCR reactions this kit is recommended when a higher yield of PCR product is needed or when amplifying longer products up to 570 bp This TB Green qPCR premix is supplied at a 2X concentration and allows easy reaction assembly by simply adding primers template and sterile distilled water intercalator based qPCR can be performed
    https://www.bioz.com/result/tb green premix ex taq ii mastermix/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tb green premix ex taq ii mastermix - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR"

    Article Title: Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR

    Journal: Data in Brief

    doi: 10.1016/j.dib.2019.104914

    Specificity of qPCR-ML (A) and qPCR-ama (B). The qPCR amplicons were run on a 2% agarose gel. Both qPCR assays were performed using three different PCR master mix: SYBR green PCR master mix, Diatheva (I); RT2 SYBR Green ROX FAST Mastermix, Qiagen (II); TB Green premix ex Taq II Mastermix, Takara Bio (III). For each master mix, DNA from L. ( L. ) infantum (0,006 ng/μl) , L. ( L. ) amazonensis (0,15 ng/μl) , Trypanosoma cruzi (0,1 ng/μl) and human DNA (30 ng/μl) were tested with the condition described in the manuscript. As negative control, a no template control was used for each qPCR run. M: ΦX174 DNA/ Bsu RI ( Hae III) Marker, 9 (ThermoFisher Scientific); NTC: no template control.
    Figure Legend Snippet: Specificity of qPCR-ML (A) and qPCR-ama (B). The qPCR amplicons were run on a 2% agarose gel. Both qPCR assays were performed using three different PCR master mix: SYBR green PCR master mix, Diatheva (I); RT2 SYBR Green ROX FAST Mastermix, Qiagen (II); TB Green premix ex Taq II Mastermix, Takara Bio (III). For each master mix, DNA from L. ( L. ) infantum (0,006 ng/μl) , L. ( L. ) amazonensis (0,15 ng/μl) , Trypanosoma cruzi (0,1 ng/μl) and human DNA (30 ng/μl) were tested with the condition described in the manuscript. As negative control, a no template control was used for each qPCR run. M: ΦX174 DNA/ Bsu RI ( Hae III) Marker, 9 (ThermoFisher Scientific); NTC: no template control.

    Techniques Used: Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis, Polymerase Chain Reaction, SYBR Green Assay, Negative Control, Marker

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    Article Snippet: .. All real-time reactions were performed on a FAST-7500 system (ABI-7500; ThermoFisher, Singapore) using a SYBR RT-PCR Kit (RR420A, TaKaRa). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Glucose affects cell viability, migration, angiogenesis and cellular adhesion of human retinal capillary endothelial cells via SPARC
    Article Snippet: .. The specific primers used are listed in . qPCR was performed by utilizing the SYBR® Premix Ex Taq (cat. no. RR420L; Takara Bio, Inc., Otsu, Japan). .. The following PCR conditions were used: Pre-denaturing at 95°C for 2 min; denaturing at 95°C for 10 sec; and annealing and polymerization at 60°C for 30 sec and 70°C for 45 sec. A total of 40 PCR cycles were performed.

    Article Title: Overaccumulation of Fat Caused Rapid Reproductive Senescence but not Loss of Ovarian Reserve in ob/ob Mice
    Article Snippet: .. Real-time PCR was performed using SYBR Premix Ex Taq (TaKaRa, Cat #: RR420A, Japan) and a Thermal Cycler Dice Real-Time System TP800 (TaKaRa, Cat #: TP800, Japan). .. Dissociation curves were plotted for all reactions to confirm amplification of a single product with the appropriate melting temperature.

    SYBR Green Assay:

    Article Title: Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR
    Article Snippet: .. Three different PCR master mix were tested: SYBR green PCR master mix (Diatheva srl, Fano, Italy), RT2 SYBR Green ROX FAST Mastermix (Qiagen, Hilden, Germany), TB Green premix ex Taq II Mastermix (Takara Bio Europe, France). .. Tubes were placed in a thermal cycler (GeneAmp PCR System 2700), and pre-amplified under the following conditions: 94 °C for 5 min, 10 cycles at 94 °C for 30 s, 60 °C for 20 s and 72 °C for 20 s. At the end of this pre-amplification step, the tubes were centrifuged for few seconds and placed in ice; the filter paper was removed and the reaction was split into two PCR tubes (20 μl each tube).

    Quantitative RT-PCR:

    Article Title: Hsa_circularRNA_0079201 suppresses chondrocyte proliferation and endochondral ossification by regulating the microRNA-140-3p/SMAD2 signaling pathway in idiopathic short stature
    Article Snippet: .. The RT-qPCR protocol used for circRNA-0079201 (TB Green® Premix Ex Taq™ II; Takara Bio, Inc.) was as follows: Initial denaturation at 95°C for 10 min, followed by 40 cycles of 95°C for 10 sec, and 60°C for 34 sec. GAPDH was used as the internal control. .. Relative mRNA expression levels of the target genes were calculated using the 2−ΔΔCq method ( ) and normalized against GAPDH, an internal control used to reduce errors, due to differences in RNA concentration and transcription efficiency.

    Article Title: Comparative transcriptome analysis reveals osmotic-regulated genes in the gill of Chinese mitten crab (Eriocheir sinensis)
    Article Snippet: .. The qRT-PCR reaction system contained 10 μL 2×SYBR Premix Ex Taq (Cat. No. RR420A; TaKaRa), 0.2 μmol/L primers. and 2 μL cDNA template. .. Reactions were performed on an ABI 7500 Real-Time PCR System (Life Tech, Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR
    Article Snippet: .. Three different PCR master mix were tested: SYBR green PCR master mix (Diatheva srl, Fano, Italy), RT2 SYBR Green ROX FAST Mastermix (Qiagen, Hilden, Germany), TB Green premix ex Taq II Mastermix (Takara Bio Europe, France). .. Tubes were placed in a thermal cycler (GeneAmp PCR System 2700), and pre-amplified under the following conditions: 94 °C for 5 min, 10 cycles at 94 °C for 30 s, 60 °C for 20 s and 72 °C for 20 s. At the end of this pre-amplification step, the tubes were centrifuged for few seconds and placed in ice; the filter paper was removed and the reaction was split into two PCR tubes (20 μl each tube).

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  • 99
    TaKaRa tb green premix ex taq ii mastermix
    Specificity of qPCR-ML (A) and qPCR-ama (B). The qPCR amplicons were run on a 2% agarose gel. Both qPCR assays were performed using three different PCR master mix: SYBR green PCR master mix, Diatheva (I); RT2 SYBR Green ROX FAST <t>Mastermix,</t> Qiagen (II); TB Green premix ex <t>Taq</t> II Mastermix, Takara Bio (III). For each master mix, DNA from L. ( L. ) infantum (0,006 ng/μl) , L. ( L. ) amazonensis (0,15 ng/μl) , Trypanosoma cruzi (0,1 ng/μl) and human DNA (30 ng/μl) were tested with the condition described in the manuscript. As negative control, a no template control was used for each qPCR run. M: ΦX174 DNA/ Bsu RI ( Hae III) Marker, 9 (ThermoFisher Scientific); NTC: no template control.
    Tb Green Premix Ex Taq Ii Mastermix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tb green premix ex taq ii mastermix/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tb green premix ex taq ii mastermix - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    TaKaRa sybr premix ex taq mastermix
    Specificity of qPCR-ML (A) and qPCR-ama (B). The qPCR amplicons were run on a 2% agarose gel. Both qPCR assays were performed using three different PCR master mix: SYBR green PCR master mix, Diatheva (I); RT2 SYBR Green ROX FAST <t>Mastermix,</t> Qiagen (II); TB Green premix ex <t>Taq</t> II Mastermix, Takara Bio (III). For each master mix, DNA from L. ( L. ) infantum (0,006 ng/μl) , L. ( L. ) amazonensis (0,15 ng/μl) , Trypanosoma cruzi (0,1 ng/μl) and human DNA (30 ng/μl) were tested with the condition described in the manuscript. As negative control, a no template control was used for each qPCR run. M: ΦX174 DNA/ Bsu RI ( Hae III) Marker, 9 (ThermoFisher Scientific); NTC: no template control.
    Sybr Premix Ex Taq Mastermix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr premix ex taq mastermix/product/TaKaRa
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    sybr premix ex taq mastermix - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Specificity of qPCR-ML (A) and qPCR-ama (B). The qPCR amplicons were run on a 2% agarose gel. Both qPCR assays were performed using three different PCR master mix: SYBR green PCR master mix, Diatheva (I); RT2 SYBR Green ROX FAST Mastermix, Qiagen (II); TB Green premix ex Taq II Mastermix, Takara Bio (III). For each master mix, DNA from L. ( L. ) infantum (0,006 ng/μl) , L. ( L. ) amazonensis (0,15 ng/μl) , Trypanosoma cruzi (0,1 ng/μl) and human DNA (30 ng/μl) were tested with the condition described in the manuscript. As negative control, a no template control was used for each qPCR run. M: ΦX174 DNA/ Bsu RI ( Hae III) Marker, 9 (ThermoFisher Scientific); NTC: no template control.

    Journal: Data in Brief

    Article Title: Data on the differentiation among Leishmania (Viannia) spp., Leishmania (Leishmania) infantum and Leishmania (Leishmania) amazonensis in Brazilian clinical samples using real-time PCR

    doi: 10.1016/j.dib.2019.104914

    Figure Lengend Snippet: Specificity of qPCR-ML (A) and qPCR-ama (B). The qPCR amplicons were run on a 2% agarose gel. Both qPCR assays were performed using three different PCR master mix: SYBR green PCR master mix, Diatheva (I); RT2 SYBR Green ROX FAST Mastermix, Qiagen (II); TB Green premix ex Taq II Mastermix, Takara Bio (III). For each master mix, DNA from L. ( L. ) infantum (0,006 ng/μl) , L. ( L. ) amazonensis (0,15 ng/μl) , Trypanosoma cruzi (0,1 ng/μl) and human DNA (30 ng/μl) were tested with the condition described in the manuscript. As negative control, a no template control was used for each qPCR run. M: ΦX174 DNA/ Bsu RI ( Hae III) Marker, 9 (ThermoFisher Scientific); NTC: no template control.

    Article Snippet: Three different PCR master mix were tested: SYBR green PCR master mix (Diatheva srl, Fano, Italy), RT2 SYBR Green ROX FAST Mastermix (Qiagen, Hilden, Germany), TB Green premix ex Taq II Mastermix (Takara Bio Europe, France).

    Techniques: Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis, Polymerase Chain Reaction, SYBR Green Assay, Negative Control, Marker