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Merck KGaA taurodeoxycholic acid
Bile salt hydrolase (BSH) enzyme activity assay. <t>Taurodeoxycholic</t> acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p
Taurodeoxycholic Acid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taurodeoxycholic acid/product/Merck KGaA
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taurodeoxycholic acid - by Bioz Stars, 2021-04
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1) Product Images from "Functional Microbiomics: Evaluation of Gut Microbiota-Bile Acid Metabolism Interactions in Health and Disease"

Article Title: Functional Microbiomics: Evaluation of Gut Microbiota-Bile Acid Metabolism Interactions in Health and Disease

Journal: Methods (San Diego, Calif.)

doi: 10.1016/j.ymeth.2018.04.028

Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p
Figure Legend Snippet: Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p

Techniques Used: Enzyme Activity Assay, Enzymatic Assay

Related Articles

Incubation:

Article Title: Bile-Salt-Hydrolases from the Probiotic Strain Lactobacillus johnsonii La1 Mediate Anti-giardial Activity in Vitro and in Vivo
Article Snippet: E. coli strains harboring pLB490, pLB491, and pLB492 were cultured in LB broth in presence of ampicillin (100 μg/ml). .. Overnight cultures were then spotted on LB agar plates supplemented with either 0.5% taurodeoxycholic acid (TDCA, Sigma-Aldrich) or 0.5% glycodeoxycholic acid (GDCA, Merck Millipore) and incubated at 37°C for 48 h. The BSH hydrolyzing activities were also monitored using purified recombinant enzymes in presence of conjugated bile salts, in solution, by measuring the liberation of amino acids (glycine or taurine) as previously described (Grill et al., ). .. A volume of 100 μl of rBSH (20 μg) was mixed with 100 μl of 2.4 g/L of each conjugated bile salts (GDCA, TDCA, glycocholic acid, or taurocholic acid) and incubated for 30 min at 37°C.

Article Title: Novel Characteristics of Mitochondrial Electron Transport Chain from Eimeria tenella
Article Snippet: .. After the last centrifugation, parasites were incubated in 4 mL of excystation medium [0.25% (w/v) trypsin (Merck, Darmstadt, Germany) and 1% (w/v) taurodeoxycholic acid (Sigma, St. Louis, MO, USA) in Hanks’ balanced salt solution (Sigma), pH 7.4], at 4 °C for overnight. ..

Article Title: Characterization of INS-15, A Metalloprotease Potentially Involved in the Invasion of Cryptosporidium parvum
Article Snippet: Two weeks after the last immunization, the rabbits were sacrificed, sera collected, and the polyclonal IgG antibodies purified using an affinity chromatographic column conjugated with INS-15 domain I or INS-15 polypeptide. .. Western Blot Analysis of Native INS-15 To assess the expression of the native INS-15, oocysts treated with 0.5% sodium hypochlorite were suspended in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin and incubated at 37 °C for 1 h. The sporozoites released were collected by centrifugation and resuspended in PBS containing 1% protease inhibitor cocktail (Merck, Darmstadt, Germany). .. They were lysed by adding a protein-loading buffer (Yeasen, Shanghai, China) and boiling for 5 min. Proteins (from ~5 × 106 sporozoites/lane) in the lysate were separated on SDS-PAGE and transferred onto nitrocellulose membranes.

Article Title: Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum
Article Snippet: The titer and specificity of the antibodies were evaluated using an enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. .. Western blot analysis of native CpMEDLE-1 To assess the expression of CpMEDLE-1, free sporozoites were prepared from oocysts that were treated with 0.5% sodium hypochlorite as described above and incubated in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin at 37 °C for 1 h. The released sporozoites were collected, washed three times by centrifugation with PBS at 13,200× g for 3 min, and re-suspended in PBS containing 1% Triton X-100 and a protease inhibitor cocktail (Merck, Darmstadt, Germany). ..

Article Title: Functional Microbiomics: Evaluation of Gut Microbiota-Bile Acid Metabolism Interactions in Health and Disease
Article Snippet: The assay was performed in a clear flat-bottomed 96-well microtitre plate and incubated at 37°C at pH 5.8 for up to 8 hours. .. In a total volume of 200μl, 500μg of faecal protein was incubated with sodium phosphate buffer (pH 5.8, final concentration of 0.02mM), and taurodeoxycholic acid (Merck, Damstadt, Germany) (at final concentration 1mM). .. To prevent evaporation during incubation, wells were overlaid with 50μl of light paraffin oil (0.85g/ml; PanReac AppliChem, Barcelona, Spain) [ ].

Purification:

Article Title: Bile-Salt-Hydrolases from the Probiotic Strain Lactobacillus johnsonii La1 Mediate Anti-giardial Activity in Vitro and in Vivo
Article Snippet: E. coli strains harboring pLB490, pLB491, and pLB492 were cultured in LB broth in presence of ampicillin (100 μg/ml). .. Overnight cultures were then spotted on LB agar plates supplemented with either 0.5% taurodeoxycholic acid (TDCA, Sigma-Aldrich) or 0.5% glycodeoxycholic acid (GDCA, Merck Millipore) and incubated at 37°C for 48 h. The BSH hydrolyzing activities were also monitored using purified recombinant enzymes in presence of conjugated bile salts, in solution, by measuring the liberation of amino acids (glycine or taurine) as previously described (Grill et al., ). .. A volume of 100 μl of rBSH (20 μg) was mixed with 100 μl of 2.4 g/L of each conjugated bile salts (GDCA, TDCA, glycocholic acid, or taurocholic acid) and incubated for 30 min at 37°C.

Recombinant:

Article Title: Bile-Salt-Hydrolases from the Probiotic Strain Lactobacillus johnsonii La1 Mediate Anti-giardial Activity in Vitro and in Vivo
Article Snippet: E. coli strains harboring pLB490, pLB491, and pLB492 were cultured in LB broth in presence of ampicillin (100 μg/ml). .. Overnight cultures were then spotted on LB agar plates supplemented with either 0.5% taurodeoxycholic acid (TDCA, Sigma-Aldrich) or 0.5% glycodeoxycholic acid (GDCA, Merck Millipore) and incubated at 37°C for 48 h. The BSH hydrolyzing activities were also monitored using purified recombinant enzymes in presence of conjugated bile salts, in solution, by measuring the liberation of amino acids (glycine or taurine) as previously described (Grill et al., ). .. A volume of 100 μl of rBSH (20 μg) was mixed with 100 μl of 2.4 g/L of each conjugated bile salts (GDCA, TDCA, glycocholic acid, or taurocholic acid) and incubated for 30 min at 37°C.

Centrifugation:

Article Title: Novel Characteristics of Mitochondrial Electron Transport Chain from Eimeria tenella
Article Snippet: .. After the last centrifugation, parasites were incubated in 4 mL of excystation medium [0.25% (w/v) trypsin (Merck, Darmstadt, Germany) and 1% (w/v) taurodeoxycholic acid (Sigma, St. Louis, MO, USA) in Hanks’ balanced salt solution (Sigma), pH 7.4], at 4 °C for overnight. ..

Article Title: Characterization of INS-15, A Metalloprotease Potentially Involved in the Invasion of Cryptosporidium parvum
Article Snippet: Two weeks after the last immunization, the rabbits were sacrificed, sera collected, and the polyclonal IgG antibodies purified using an affinity chromatographic column conjugated with INS-15 domain I or INS-15 polypeptide. .. Western Blot Analysis of Native INS-15 To assess the expression of the native INS-15, oocysts treated with 0.5% sodium hypochlorite were suspended in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin and incubated at 37 °C for 1 h. The sporozoites released were collected by centrifugation and resuspended in PBS containing 1% protease inhibitor cocktail (Merck, Darmstadt, Germany). .. They were lysed by adding a protein-loading buffer (Yeasen, Shanghai, China) and boiling for 5 min. Proteins (from ~5 × 106 sporozoites/lane) in the lysate were separated on SDS-PAGE and transferred onto nitrocellulose membranes.

Article Title: Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum
Article Snippet: The titer and specificity of the antibodies were evaluated using an enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. .. Western blot analysis of native CpMEDLE-1 To assess the expression of CpMEDLE-1, free sporozoites were prepared from oocysts that were treated with 0.5% sodium hypochlorite as described above and incubated in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin at 37 °C for 1 h. The released sporozoites were collected, washed three times by centrifugation with PBS at 13,200× g for 3 min, and re-suspended in PBS containing 1% Triton X-100 and a protease inhibitor cocktail (Merck, Darmstadt, Germany). ..

Western Blot:

Article Title: Characterization of INS-15, A Metalloprotease Potentially Involved in the Invasion of Cryptosporidium parvum
Article Snippet: Two weeks after the last immunization, the rabbits were sacrificed, sera collected, and the polyclonal IgG antibodies purified using an affinity chromatographic column conjugated with INS-15 domain I or INS-15 polypeptide. .. Western Blot Analysis of Native INS-15 To assess the expression of the native INS-15, oocysts treated with 0.5% sodium hypochlorite were suspended in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin and incubated at 37 °C for 1 h. The sporozoites released were collected by centrifugation and resuspended in PBS containing 1% protease inhibitor cocktail (Merck, Darmstadt, Germany). .. They were lysed by adding a protein-loading buffer (Yeasen, Shanghai, China) and boiling for 5 min. Proteins (from ~5 × 106 sporozoites/lane) in the lysate were separated on SDS-PAGE and transferred onto nitrocellulose membranes.

Article Title: Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum
Article Snippet: The titer and specificity of the antibodies were evaluated using an enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. .. Western blot analysis of native CpMEDLE-1 To assess the expression of CpMEDLE-1, free sporozoites were prepared from oocysts that were treated with 0.5% sodium hypochlorite as described above and incubated in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin at 37 °C for 1 h. The released sporozoites were collected, washed three times by centrifugation with PBS at 13,200× g for 3 min, and re-suspended in PBS containing 1% Triton X-100 and a protease inhibitor cocktail (Merck, Darmstadt, Germany). ..

Expressing:

Article Title: Characterization of INS-15, A Metalloprotease Potentially Involved in the Invasion of Cryptosporidium parvum
Article Snippet: Two weeks after the last immunization, the rabbits were sacrificed, sera collected, and the polyclonal IgG antibodies purified using an affinity chromatographic column conjugated with INS-15 domain I or INS-15 polypeptide. .. Western Blot Analysis of Native INS-15 To assess the expression of the native INS-15, oocysts treated with 0.5% sodium hypochlorite were suspended in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin and incubated at 37 °C for 1 h. The sporozoites released were collected by centrifugation and resuspended in PBS containing 1% protease inhibitor cocktail (Merck, Darmstadt, Germany). .. They were lysed by adding a protein-loading buffer (Yeasen, Shanghai, China) and boiling for 5 min. Proteins (from ~5 × 106 sporozoites/lane) in the lysate were separated on SDS-PAGE and transferred onto nitrocellulose membranes.

Article Title: Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum
Article Snippet: The titer and specificity of the antibodies were evaluated using an enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. .. Western blot analysis of native CpMEDLE-1 To assess the expression of CpMEDLE-1, free sporozoites were prepared from oocysts that were treated with 0.5% sodium hypochlorite as described above and incubated in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin at 37 °C for 1 h. The released sporozoites were collected, washed three times by centrifugation with PBS at 13,200× g for 3 min, and re-suspended in PBS containing 1% Triton X-100 and a protease inhibitor cocktail (Merck, Darmstadt, Germany). ..

Protease Inhibitor:

Article Title: Characterization of INS-15, A Metalloprotease Potentially Involved in the Invasion of Cryptosporidium parvum
Article Snippet: Two weeks after the last immunization, the rabbits were sacrificed, sera collected, and the polyclonal IgG antibodies purified using an affinity chromatographic column conjugated with INS-15 domain I or INS-15 polypeptide. .. Western Blot Analysis of Native INS-15 To assess the expression of the native INS-15, oocysts treated with 0.5% sodium hypochlorite were suspended in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin and incubated at 37 °C for 1 h. The sporozoites released were collected by centrifugation and resuspended in PBS containing 1% protease inhibitor cocktail (Merck, Darmstadt, Germany). .. They were lysed by adding a protein-loading buffer (Yeasen, Shanghai, China) and boiling for 5 min. Proteins (from ~5 × 106 sporozoites/lane) in the lysate were separated on SDS-PAGE and transferred onto nitrocellulose membranes.

Article Title: Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum
Article Snippet: The titer and specificity of the antibodies were evaluated using an enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. .. Western blot analysis of native CpMEDLE-1 To assess the expression of CpMEDLE-1, free sporozoites were prepared from oocysts that were treated with 0.5% sodium hypochlorite as described above and incubated in PBS buffer containing 0.75% taurodeoxycholic acid and 0.25% trypsin at 37 °C for 1 h. The released sporozoites were collected, washed three times by centrifugation with PBS at 13,200× g for 3 min, and re-suspended in PBS containing 1% Triton X-100 and a protease inhibitor cocktail (Merck, Darmstadt, Germany). ..

Concentration Assay:

Article Title: Functional Microbiomics: Evaluation of Gut Microbiota-Bile Acid Metabolism Interactions in Health and Disease
Article Snippet: The assay was performed in a clear flat-bottomed 96-well microtitre plate and incubated at 37°C at pH 5.8 for up to 8 hours. .. In a total volume of 200μl, 500μg of faecal protein was incubated with sodium phosphate buffer (pH 5.8, final concentration of 0.02mM), and taurodeoxycholic acid (Merck, Damstadt, Germany) (at final concentration 1mM). .. To prevent evaporation during incubation, wells were overlaid with 50μl of light paraffin oil (0.85g/ml; PanReac AppliChem, Barcelona, Spain) [ ].

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    Merck KGaA taurodeoxycholic acid
    Bile salt hydrolase (BSH) enzyme activity assay. <t>Taurodeoxycholic</t> acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p
    Taurodeoxycholic Acid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taurodeoxycholic acid/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taurodeoxycholic acid - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA taurodeoxycholic acid tdca
    Bile salt hydrolase (BSH) enzyme activity assay. <t>Taurodeoxycholic</t> acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p
    Taurodeoxycholic Acid Tdca, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taurodeoxycholic acid tdca/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taurodeoxycholic acid tdca - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p

    Journal: Methods (San Diego, Calif.)

    Article Title: Functional Microbiomics: Evaluation of Gut Microbiota-Bile Acid Metabolism Interactions in Health and Disease

    doi: 10.1016/j.ymeth.2018.04.028

    Figure Lengend Snippet: Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p

    Article Snippet: In a total volume of 200μl, 500μg of faecal protein was incubated with sodium phosphate buffer (pH 5.8, final concentration of 0.02mM), and taurodeoxycholic acid (Merck, Damstadt, Germany) (at final concentration 1mM).

    Techniques: Enzyme Activity Assay, Enzymatic Assay