target grna  (Millipore)


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    Structured Review

    Millipore target grna
    Target Grna, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/target grna/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    target grna - by Bioz Stars, 2020-05
    93/100 stars

    Related Products / Commonly Used Together

    c57bl/6 mice
    m2 media
    femtojet micromanipulator
    sacas9 mrna

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    Related Articles

    Injection:

    Article Title: Unveil the transcriptional landscape at the Cryptococcus-host axis in mice and nonhuman primates
    Article Snippet: .. Zygotes of C57BL/6 mice were injected with saCas9 mRNA and target gRNA in M2 media (Millipore) using a FemtoJet micromanipulator (Eppendorf, Germany). .. Following microinjection, zygotes were transferred to pseudo pregnant female mice.

    Mouse Assay:

    Article Title: Unveil the transcriptional landscape at the Cryptococcus-host axis in mice and nonhuman primates
    Article Snippet: .. Zygotes of C57BL/6 mice were injected with saCas9 mRNA and target gRNA in M2 media (Millipore) using a FemtoJet micromanipulator (Eppendorf, Germany). .. Following microinjection, zygotes were transferred to pseudo pregnant female mice.

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  • 92
    Millipore non targeting grna
    Data analysis for the TXTL-based <t>PAM</t> assay performed on the Cas9 from Neisseria meningitides (NmeCas9) expressed from linear DNA. (A) Fluorescence data from the GFP cleavage assay of NmeCas9 using an NNNNGATT PAM. The red and gray lines indicate TXTL reactions containing NmeCas9 with a <t>gRNA</t> targeting the reporter plasmid pTJ247 or a non-targeting gRNA, respectively. Both reactions had similar GFP expression rates, but after ~1 h, the reaction containing the targeting gRNA began to show a decrease in GFP production. (B) Plot showing the log 2 -fold change in the nucleotide frequency across the 10-nucleotide PAM library. The depletion shows the strong NNNNGATTNN with a slight bias of D in the 9th and 10th positions. Note the inverted y-axis. (C) The average fold-change of 10-mers for selected PAM motifs in the 5th-10th position is shown. While a NNNNGATTNN PAM was strong, having an A in the 9th position increases cleavage efficiency compared to the other nucleotides, and having a T in the 10th position slightly increased cleavage for NNNNGATTNN PAMs. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Non Targeting Grna, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non targeting grna/product/Millipore
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    non targeting grna - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Millipore grna targetting vector
    Data analysis for the TXTL-based <t>PAM</t> assay performed on the Cas9 from Neisseria meningitides (NmeCas9) expressed from linear DNA. (A) Fluorescence data from the GFP cleavage assay of NmeCas9 using an NNNNGATT PAM. The red and gray lines indicate TXTL reactions containing NmeCas9 with a <t>gRNA</t> targeting the reporter plasmid pTJ247 or a non-targeting gRNA, respectively. Both reactions had similar GFP expression rates, but after ~1 h, the reaction containing the targeting gRNA began to show a decrease in GFP production. (B) Plot showing the log 2 -fold change in the nucleotide frequency across the 10-nucleotide PAM library. The depletion shows the strong NNNNGATTNN with a slight bias of D in the 9th and 10th positions. Note the inverted y-axis. (C) The average fold-change of 10-mers for selected PAM motifs in the 5th-10th position is shown. While a NNNNGATTNN PAM was strong, having an A in the 9th position increases cleavage efficiency compared to the other nucleotides, and having a T in the 10th position slightly increased cleavage for NNNNGATTNN PAMs. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Grna Targetting Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grna targetting vector/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    grna targetting vector - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    Image Search Results


    Data analysis for the TXTL-based PAM assay performed on the Cas9 from Neisseria meningitides (NmeCas9) expressed from linear DNA. (A) Fluorescence data from the GFP cleavage assay of NmeCas9 using an NNNNGATT PAM. The red and gray lines indicate TXTL reactions containing NmeCas9 with a gRNA targeting the reporter plasmid pTJ247 or a non-targeting gRNA, respectively. Both reactions had similar GFP expression rates, but after ~1 h, the reaction containing the targeting gRNA began to show a decrease in GFP production. (B) Plot showing the log 2 -fold change in the nucleotide frequency across the 10-nucleotide PAM library. The depletion shows the strong NNNNGATTNN with a slight bias of D in the 9th and 10th positions. Note the inverted y-axis. (C) The average fold-change of 10-mers for selected PAM motifs in the 5th-10th position is shown. While a NNNNGATTNN PAM was strong, having an A in the 9th position increases cleavage efficiency compared to the other nucleotides, and having a T in the 10th position slightly increased cleavage for NNNNGATTNN PAMs. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Methods (San Diego, Calif.)

    Article Title: A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs

    doi: 10.1016/j.ymeth.2018.02.016

    Figure Lengend Snippet: Data analysis for the TXTL-based PAM assay performed on the Cas9 from Neisseria meningitides (NmeCas9) expressed from linear DNA. (A) Fluorescence data from the GFP cleavage assay of NmeCas9 using an NNNNGATT PAM. The red and gray lines indicate TXTL reactions containing NmeCas9 with a gRNA targeting the reporter plasmid pTJ247 or a non-targeting gRNA, respectively. Both reactions had similar GFP expression rates, but after ~1 h, the reaction containing the targeting gRNA began to show a decrease in GFP production. (B) Plot showing the log 2 -fold change in the nucleotide frequency across the 10-nucleotide PAM library. The depletion shows the strong NNNNGATTNN with a slight bias of D in the 9th and 10th positions. Note the inverted y-axis. (C) The average fold-change of 10-mers for selected PAM motifs in the 5th-10th position is shown. While a NNNNGATTNN PAM was strong, having an A in the 9th position increases cleavage efficiency compared to the other nucleotides, and having a T in the 10th position slightly increased cleavage for NNNNGATTNN PAMs. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: myTXTL In vitro TX-TL protein expression kit [ ] (Arbor Biosciences, 507096) 50 nM randomized PAM library 30 nM pET vector expressing the Cas nuclease of interest 20 nM DNA expressing a gRNA targeting the protospacer in the PAM library 20 nM DNA expressing a non-targeting gRNA 50 μM Chi6 DNA 96 well V-bottom plates (e.g. MilliporeSigma, CLS3357) Cap mat (e.g. MilliporeSigma, CLS3080) 20 nM p70a-T7RNAP 50 nM reporter plasmid 50 mM IPTG (e.g. ThermoFisher, R1171) Fluorescent plate reader capable of measuring GFP (e.g. BioTek H1)

    Techniques: Fluorescence, Cleavage Assay, Plasmid Preparation, Expressing