taqman universal pcr master mix kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher taqman universal pcr master mix kit
    Taqman Universal Pcr Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal pcr master mix kit/product/Thermo Fisher
    Average 99 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    taqman universal pcr master mix kit - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: .. Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector. .. The amplification conditions on the PE Applied Biosystems 7700 sequence detector were the following: 2 min at 50°C, 10 min at 95°C, 40 cycles of 95°C for 15 s and 60°C for 1 min for all reactions.

    Article Title: TMP778, a selective inhibitor of RORγt, suppresses experimental autoimmune uveitis development, but affects both Th17 and Th1 cell populations
    Article Snippet: Primer-probe sets for qPCR for T-bet (Tbx21) and Rorγt were purchased from Applied Biosystems. .. PCR parameters were as recommended for the TaqMan Universal PCR Master Mix kit (Applied Biosystems).

    Article Title: Phenotype Switching by Inflammation-Inducing Polarized Th17 Cells, but not by Th1 Cells 1
    Article Snippet: Paragraph title: Quantitative PCR Analysis ... PCR parameters were as recommended for the TaqMan Universal PCR master mix kit (Applied Biosystems).

    Article Title: Signals in Stem Cell Differentiation on Fluorapatite-Modified Scaffolds
    Article Snippet: Paragraph title: Real-time Polymerase Chain Reaction ... The TaqMan Universal PCR Master Mix Kit (Applied Biosystems) was used following the manufacturer’s protocol.

    Amplification:

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector. .. The amplification conditions on the PE Applied Biosystems 7700 sequence detector were the following: 2 min at 50°C, 10 min at 95°C, 40 cycles of 95°C for 15 s and 60°C for 1 min for all reactions.

    Fluorescence:

    Article Title: Phenotype Switching by Inflammation-Inducing Polarized Th17 Cells, but not by Th1 Cells 1
    Article Snippet: Fluorescence-labeled probes used are: ROR-γt, 5′-AAGGGCTTCTTCCGCCGCAGCCA GCAG-BHQ-1-3′; ROR-α: 5′-CCTTGCCCAGAACATATCCA AATCCCA-BHQ-1-3′. .. PCR parameters were as recommended for the TaqMan Universal PCR master mix kit (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: .. Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector. .. The amplification conditions on the PE Applied Biosystems 7700 sequence detector were the following: 2 min at 50°C, 10 min at 95°C, 40 cycles of 95°C for 15 s and 60°C for 1 min for all reactions.

    Article Title: TMP778, a selective inhibitor of RORγt, suppresses experimental autoimmune uveitis development, but affects both Th17 and Th1 cell populations
    Article Snippet: .. PCR parameters were as recommended for the TaqMan Universal PCR Master Mix kit (Applied Biosystems). .. Where non-normal distributions were compared, the Mann-Whitney U test was used ( ).

    Article Title: Phenotype Switching by Inflammation-Inducing Polarized Th17 Cells, but not by Th1 Cells 1
    Article Snippet: .. PCR parameters were as recommended for the TaqMan Universal PCR master mix kit (Applied Biosystems). .. Recipient eyes were collected on days 5 and 10 post-cell transfer and processed for histological analysis by H & E staining, using conventional methods.

    Article Title: Signals in Stem Cell Differentiation on Fluorapatite-Modified Scaffolds
    Article Snippet: .. The TaqMan Universal PCR Master Mix Kit (Applied Biosystems) was used following the manufacturer’s protocol. ..

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: .. Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR. .. Primer/probe combinations were produced by the Custom Oligonucleotide Synthesis Service (Applied Biosystems).

    Isolation:

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction. .. Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector.

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: Human keratinocyte HaCaT cells were harvested, and total cellular RNA was purified using a Miniprep RNA isolation kit according to the manufacturer’s instruction (Qiagen, Chatsworth, CA). .. Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR.

    Sequencing:

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: .. Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector. .. The amplification conditions on the PE Applied Biosystems 7700 sequence detector were the following: 2 min at 50°C, 10 min at 95°C, 40 cycles of 95°C for 15 s and 60°C for 1 min for all reactions.

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: .. Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR. .. Primer/probe combinations were produced by the Custom Oligonucleotide Synthesis Service (Applied Biosystems).

    Cell Culture:

    Article Title: Phenotype Switching by Inflammation-Inducing Polarized Th17 Cells, but not by Th1 Cells 1
    Article Snippet: Total RNA was extracted from cultured cells or recipient eyes with TriZol (Invitrogen-Life Technologies, Carlsbad, CA). .. PCR parameters were as recommended for the TaqMan Universal PCR master mix kit (Applied Biosystems).

    Purification:

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: Human keratinocyte HaCaT cells were harvested, and total cellular RNA was purified using a Miniprep RNA isolation kit according to the manufacturer’s instruction (Qiagen, Chatsworth, CA). .. Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR.

    Produced:

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR. .. Primer/probe combinations were produced by the Custom Oligonucleotide Synthesis Service (Applied Biosystems).

    Oligonucleotide Synthesis:

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR. .. Primer/probe combinations were produced by the Custom Oligonucleotide Synthesis Service (Applied Biosystems).

    Quantitation Assay:

    Article Title: Signals in Stem Cell Differentiation on Fluorapatite-Modified Scaffolds
    Article Snippet: RT-PCR quantitation was carried out by the ViiA 7 system (Life Tech). .. The TaqMan Universal PCR Master Mix Kit (Applied Biosystems) was used following the manufacturer’s protocol.

    Quantitative RT-PCR:

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: .. Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR. .. Primer/probe combinations were produced by the Custom Oligonucleotide Synthesis Service (Applied Biosystems).

    Expressing:

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: Paragraph title: Analysis of mRNA Expression ... Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix kit (PE Applied Biosystems) on a PE Applied Biosystems 7700 sequence detector.

    Article Title: TMP778, a selective inhibitor of RORγt, suppresses experimental autoimmune uveitis development, but affects both Th17 and Th1 cell populations
    Article Snippet: Paragraph title: Quantitative (q)PCR analysis of gene expression ... PCR parameters were as recommended for the TaqMan Universal PCR Master Mix kit (Applied Biosystems).

    Article Title: Phenotype Switching by Inflammation-Inducing Polarized Th17 Cells, but not by Th1 Cells 1
    Article Snippet: Primer-probe sets for real-time PCR quantifying expression of mouse T-bet, IL-12, IL-23 and GAPDH or β-actin (internal control) were purchased from Applied Biosystems (Foster City, CA). .. PCR parameters were as recommended for the TaqMan Universal PCR master mix kit (Applied Biosystems).

    Article Title: Signals in Stem Cell Differentiation on Fluorapatite-Modified Scaffolds
    Article Snippet: The TaqMan Universal PCR Master Mix Kit (Applied Biosystems) was used following the manufacturer’s protocol. .. Relative gene expression values were calculated by ∆∆CT-based fold-change calculations.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Signals in Stem Cell Differentiation on Fluorapatite-Modified Scaffolds
    Article Snippet: RT-PCR quantitation was carried out by the ViiA 7 system (Life Tech). .. The TaqMan Universal PCR Master Mix Kit (Applied Biosystems) was used following the manufacturer’s protocol.

    Article Title: Epidermal Growth Factor Receptor Is a Critical Mediator of Ultraviolet B Irradiation-Induced Signal Transduction in Immortalized Human Keratinocyte HaCaT Cells
    Article Snippet: Paragraph title: Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) Measurements of c-Fos and c-Jun mRNA ... Real-time RT-PCR was performed using a TaqMan Universal PCR Master Mix kit (Applied Biosystems) and a 7700 Sequence Detector (Applied Biosystems) was used to run the PCR.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher taqman universal master mix ii
    Taqman Universal Master Mix Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 982 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal master mix ii/product/Thermo Fisher
    Average 90 stars, based on 982 article reviews
    Price from $9.99 to $1999.99
    taqman universal master mix ii - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results