taqman universal pcr master mix kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    TaqMan Universal PCR Master Mix
    Description:
    Alternative Product Try TaqMan Fast Advanced Master Mix our highest performance probe based master mix With TaqMan Fast Advanced Master Mix we ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis Applied Biosystems TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5 nuclease DNA applications Applied Biosystems reagents have been validated with TaqMan Assays and Applied Biosystems real time systems to ensure sensitive accurate and reliable performance every time • Contains AmpliTaq Gold DNA polymerase to provide a better yield and a more robust 5 nuclease assay than AmpliTaq DNA polymerase• Includes a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real time PCR instruments • Buffer enhancements guarantee performance and reliability in all applications even with G C rich sequences• AmpErase UNG protects against subsequent re amplification from PCR products containing dU to minimize carry over contamination• Rapid assay development guidelines are provided to minimize optimization time
    Catalog Number:
    4304437
    Price:
    None
    Applications:
    Enzymes & Master Mixes for Real-Time PCR|Industrial & Applied Science|PCR & Real-Time PCR|PCR-Based Drug Discovery Assays|Pharma & Biopharma|RNAi, Epigenetics & Non-Coding RNA Research|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real Time PCR-Based miRNA Analysis|miRNA & Non-Coding RNA Analysis|Target & Lead Identification & Validation|Gene Expression Analysis & Genotyping
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher taqman universal pcr master mix kit
    CKS1B and CKS2 expression is higher in diagnostic bone marrow samples from patients carrying MLL -translocations compared to PBMCs from healthy controls. RNA extracted from human umbilical cord blood CD34 + cells, PBMCs from healthy donors, and leukaemic patient samples, were assessed for (A) CKS1B and (B) CKS2 expression by <t>TaqMan</t> quantitative <t>PCR.</t> Three independent control genes were used ( ABL , GUS , B2M ) to measure relative RNA abundance. Values are represented as relative gene expression versus GUS control. Each point represents one patient. Median bars with standard deviation are presented for each set of samples. A Student's t -test was used to assess significance, and * indicates significance versus CD34 + cells, and § indicates significance versus PBMCs.
    Alternative Product Try TaqMan Fast Advanced Master Mix our highest performance probe based master mix With TaqMan Fast Advanced Master Mix we ve taken the best of TaqMan Universal PCR Master Mix and added additional capabilities for your gene expression analysis Applied Biosystems TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5 nuclease DNA applications Applied Biosystems reagents have been validated with TaqMan Assays and Applied Biosystems real time systems to ensure sensitive accurate and reliable performance every time • Contains AmpliTaq Gold DNA polymerase to provide a better yield and a more robust 5 nuclease assay than AmpliTaq DNA polymerase• Includes a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real time PCR instruments • Buffer enhancements guarantee performance and reliability in all applications even with G C rich sequences• AmpErase UNG protects against subsequent re amplification from PCR products containing dU to minimize carry over contamination• Rapid assay development guidelines are provided to minimize optimization time
    https://www.bioz.com/result/taqman universal pcr master mix kit/product/Thermo Fisher
    Average 99 stars, based on 183 article reviews
    Price from $9.99 to $1999.99
    taqman universal pcr master mix kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "The Cks1/Cks2 axis fine-tunes Mll1 expression and is crucial for MLL-rearranged leukaemia cell viability"

    Article Title: The Cks1/Cks2 axis fine-tunes Mll1 expression and is crucial for MLL-rearranged leukaemia cell viability

    Journal: Biochimica et Biophysica Acta

    doi: 10.1016/j.bbamcr.2017.09.009

    CKS1B and CKS2 expression is higher in diagnostic bone marrow samples from patients carrying MLL -translocations compared to PBMCs from healthy controls. RNA extracted from human umbilical cord blood CD34 + cells, PBMCs from healthy donors, and leukaemic patient samples, were assessed for (A) CKS1B and (B) CKS2 expression by TaqMan quantitative PCR. Three independent control genes were used ( ABL , GUS , B2M ) to measure relative RNA abundance. Values are represented as relative gene expression versus GUS control. Each point represents one patient. Median bars with standard deviation are presented for each set of samples. A Student's t -test was used to assess significance, and * indicates significance versus CD34 + cells, and § indicates significance versus PBMCs.
    Figure Legend Snippet: CKS1B and CKS2 expression is higher in diagnostic bone marrow samples from patients carrying MLL -translocations compared to PBMCs from healthy controls. RNA extracted from human umbilical cord blood CD34 + cells, PBMCs from healthy donors, and leukaemic patient samples, were assessed for (A) CKS1B and (B) CKS2 expression by TaqMan quantitative PCR. Three independent control genes were used ( ABL , GUS , B2M ) to measure relative RNA abundance. Values are represented as relative gene expression versus GUS control. Each point represents one patient. Median bars with standard deviation are presented for each set of samples. A Student's t -test was used to assess significance, and * indicates significance versus CD34 + cells, and § indicates significance versus PBMCs.

    Techniques Used: Expressing, Diagnostic Assay, Real-time Polymerase Chain Reaction, Standard Deviation

    2) Product Images from "Preventative oral methylthioadenosine is anti-inflammatory and reduces DSS-induced colitis in mice"

    Article Title: Preventative oral methylthioadenosine is anti-inflammatory and reduces DSS-induced colitis in mice

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.00549.2011

    MTA reduces upregulation of gene expression. Mice were given 3% DSS for 5 days and 150 mg/kg body wt MTA for 7 days via the drinking water. After euthanasia, colonic RNA was extracted for gene expression changes using TaqMan probes for real-time PCR (Con
    Figure Legend Snippet: MTA reduces upregulation of gene expression. Mice were given 3% DSS for 5 days and 150 mg/kg body wt MTA for 7 days via the drinking water. After euthanasia, colonic RNA was extracted for gene expression changes using TaqMan probes for real-time PCR (Con

    Techniques Used: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction

    3) Product Images from "Global Gene Expression Profiling of Dimethylnitrosamine-Induced Liver Fibrosis: From Pathological and Biochemical Data to Microarray Analysis"

    Article Title: Global Gene Expression Profiling of Dimethylnitrosamine-Induced Liver Fibrosis: From Pathological and Biochemical Data to Microarray Analysis

    Journal: Gene Expression

    doi:

    ). (C) The hierarchical clustering results of the three biology processes are: metabolism, cell growth and/or maintenance and response stimulus. These diagrams are formatted as rows representing individual transcripts and columns representing time course sample. The color in each cell reflects the expression level of the corresponding sample relative to its mean expression level and the scale extends from fluorescence ratios of 0.25 to 4 relative to the mean level for all samples. (D) The comparison of Timpl expression between the Q-RT-PCR results and microarray data. The TaqMan® assays were conducted in triplicate for each sample, and a mean value was used for calculation of expression levels (marked by the square). To standardize the quantification of the Timpl , 18S rRNA from each sample was quantified at the same time as the target gene and a log scale was used as indicated on the right side of plot. For the two Timpl transcripts, rc_AI169327_at and rc_AI169327_g_at (marked by circle and triangle), the expression levels of the microarray data were relative to the mean of all gene expression levels and the scale is indicated on the left side of plot. The Pearson correlation coefficients ( r ), which compared the Q-RT-PCR result and the microarray data of two Timpl transcripts (rc_AI169327_at and rc_AI169327_g_at), were 0.79 and 0.92, respectively. (E) Endogenous Spp1 protein expression pattern in DMN-induced rat liver samples. Rat liver samples were lysed and 50 itg protein lysates were subjected to immunoblot analysis with antibody against Spp1 and Actb. Spp1 was significantly overexpressed at the protein level after the fourth week of DMN treatment.
    Figure Legend Snippet: ). (C) The hierarchical clustering results of the three biology processes are: metabolism, cell growth and/or maintenance and response stimulus. These diagrams are formatted as rows representing individual transcripts and columns representing time course sample. The color in each cell reflects the expression level of the corresponding sample relative to its mean expression level and the scale extends from fluorescence ratios of 0.25 to 4 relative to the mean level for all samples. (D) The comparison of Timpl expression between the Q-RT-PCR results and microarray data. The TaqMan® assays were conducted in triplicate for each sample, and a mean value was used for calculation of expression levels (marked by the square). To standardize the quantification of the Timpl , 18S rRNA from each sample was quantified at the same time as the target gene and a log scale was used as indicated on the right side of plot. For the two Timpl transcripts, rc_AI169327_at and rc_AI169327_g_at (marked by circle and triangle), the expression levels of the microarray data were relative to the mean of all gene expression levels and the scale is indicated on the left side of plot. The Pearson correlation coefficients ( r ), which compared the Q-RT-PCR result and the microarray data of two Timpl transcripts (rc_AI169327_at and rc_AI169327_g_at), were 0.79 and 0.92, respectively. (E) Endogenous Spp1 protein expression pattern in DMN-induced rat liver samples. Rat liver samples were lysed and 50 itg protein lysates were subjected to immunoblot analysis with antibody against Spp1 and Actb. Spp1 was significantly overexpressed at the protein level after the fourth week of DMN treatment.

    Techniques Used: Expressing, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Microarray

    4) Product Images from "B-vitamin deficiency is protective against DSS-induced colitis in mice"

    Article Title: B-vitamin deficiency is protective against DSS-induced colitis in mice

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.00076.2011

    Gene expression changes. Mice were given the control or B 6 -B 12 -deficient diet for 2 wk followed by 5 days of 3% DSS. After euthanasia, colonic RNA was extracted for gene expression changes using TaqMan probes for real-time PCR (Con n = 10, Def n = 10, Con+3% n = 9, Def+3% n = 8). As expected, expression of TNF-α, inducible nitric oxide synthase (iNOS), and IL-10 were significantly increased in DSS mice compared with non-DSS controls ( P
    Figure Legend Snippet: Gene expression changes. Mice were given the control or B 6 -B 12 -deficient diet for 2 wk followed by 5 days of 3% DSS. After euthanasia, colonic RNA was extracted for gene expression changes using TaqMan probes for real-time PCR (Con n = 10, Def n = 10, Con+3% n = 9, Def+3% n = 8). As expected, expression of TNF-α, inducible nitric oxide synthase (iNOS), and IL-10 were significantly increased in DSS mice compared with non-DSS controls ( P

    Techniques Used: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction

    5) Product Images from "The Cks1/Cks2 axis fine-tunes Mll1 expression and is crucial for MLL-rearranged leukaemia cell viability"

    Article Title: The Cks1/Cks2 axis fine-tunes Mll1 expression and is crucial for MLL-rearranged leukaemia cell viability

    Journal: Biochimica et Biophysica Acta

    doi: 10.1016/j.bbamcr.2017.09.009

    CKS1B and CKS2 expression is higher in diagnostic bone marrow samples from patients carrying MLL -translocations compared to PBMCs from healthy controls. RNA extracted from human umbilical cord blood CD34 + cells, PBMCs from healthy donors, and leukaemic patient samples, were assessed for (A) CKS1B and (B) CKS2 expression by TaqMan quantitative PCR. Three independent control genes were used ( ABL , GUS , B2M ) to measure relative RNA abundance. Values are represented as relative gene expression versus GUS control. Each point represents one patient. Median bars with standard deviation are presented for each set of samples. A Student's t -test was used to assess significance, and * indicates significance versus CD34 + cells, and § indicates significance versus PBMCs.
    Figure Legend Snippet: CKS1B and CKS2 expression is higher in diagnostic bone marrow samples from patients carrying MLL -translocations compared to PBMCs from healthy controls. RNA extracted from human umbilical cord blood CD34 + cells, PBMCs from healthy donors, and leukaemic patient samples, were assessed for (A) CKS1B and (B) CKS2 expression by TaqMan quantitative PCR. Three independent control genes were used ( ABL , GUS , B2M ) to measure relative RNA abundance. Values are represented as relative gene expression versus GUS control. Each point represents one patient. Median bars with standard deviation are presented for each set of samples. A Student's t -test was used to assess significance, and * indicates significance versus CD34 + cells, and § indicates significance versus PBMCs.

    Techniques Used: Expressing, Diagnostic Assay, Real-time Polymerase Chain Reaction, Standard Deviation

    6) Product Images from "MuSK Expressed in the Brain Mediates Cholinergic Responses, Synaptic Plasticity, and Memory Formation"

    Article Title: MuSK Expressed in the Brain Mediates Cholinergic Responses, Synaptic Plasticity, and Memory Formation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1674-06.2006

    MuSK and agrin expression is increased in rat hippocampus after IA training. A , TaqMan-PCR analysis of MuSK on hippocampal extracts taken from rats trained in IA at 0 h−, 20 h−, and 20 h+. The concentration of mRNA was normalized against that of GAPDH. Data are expressed as mean fold change ± SEM of the ratio 20 h+:0 h− versus 20 h−:0 h− (∗ p
    Figure Legend Snippet: MuSK and agrin expression is increased in rat hippocampus after IA training. A , TaqMan-PCR analysis of MuSK on hippocampal extracts taken from rats trained in IA at 0 h−, 20 h−, and 20 h+. The concentration of mRNA was normalized against that of GAPDH. Data are expressed as mean fold change ± SEM of the ratio 20 h+:0 h− versus 20 h−:0 h− (∗ p

    Techniques Used: Expressing, IA, Polymerase Chain Reaction, Concentration Assay

    Related Articles

    Amplification:

    Article Title: Neuronal MicroRNA Deregulation in Response to Alzheimer's Disease Amyloid-?
    Article Snippet: .. 10 ng of total RNA was used in the RT reaction and the transcribed cDNA was then used for subsequent PCR amplification using TaqMan 2X Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) as described by the manufacturer. .. Assays were run on an Mx3000P thermocycler (Stratagene) as follows: 95°C for 10 min, and 40 cycles at 95°C for 151s followed by 60°C for 1 min. To avoid any miRNA degradation, RNA extractions, reverse transcription reactions and real-time runs were all performed on the same day.

    Article Title: Dysregulation of miR-155-5p and miR-200-3p and the Anti-Non-Bilayer Phospholipid Arrangement Antibodies Favor the Development of Lupus in Three Novel Murine Lupus Models
    Article Snippet: .. The PCR amplification was run as described by the TaqMan Universal PCR Master Mix manual (Applied Biosystems) for the following differentially expressed miRNAs, as determined by PCR array analysis: miR-21a-5p, miR-125a-5p, miR-142-3p/5p, miR-146b/5p, miR-155-5p, and miR-200a-3p. ..

    Polymerase Chain Reaction:

    Article Title: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/?-catenin signaling pathway
    Article Snippet: .. The 100 ng cDNA products were used for quantitative real-time PCR performed using TaqMan Universal PCR Master Mix (Applied Biosystems) and TaqMan MGB primers [VEGFR-2 (Hs00911700_m1) and GAPDH (Hs99999905_m1)] with an ABI Prism 7500 (Applied Biosystems). ..

    Article Title: Cytomegalovirus pp71 Protein Is Expressed in Human Glioblastoma and Promotes Pro-Angiogenic Signaling by Activation of Stem Cell Factor
    Article Snippet: .. All assays were performed with Applied Biosystems TaqMan FAST Universal PCR Master Mix and the 7500 fast real-time PCR system. .. When necessary, standard curves were generated with serial dilutions of Ad169 viral DNA (Advanced Biotechnologies) or human genomic DNA (Applied Biosystems).

    Article Title: Neuronal MicroRNA Deregulation in Response to Alzheimer's Disease Amyloid-?
    Article Snippet: .. 10 ng of total RNA was used in the RT reaction and the transcribed cDNA was then used for subsequent PCR amplification using TaqMan 2X Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) as described by the manufacturer. .. Assays were run on an Mx3000P thermocycler (Stratagene) as follows: 95°C for 10 min, and 40 cycles at 95°C for 151s followed by 60°C for 1 min. To avoid any miRNA degradation, RNA extractions, reverse transcription reactions and real-time runs were all performed on the same day.

    Article Title: Neutrophil Chemokines Secreted by Tumor Cells Mount a Lung Antimetastatic Response during Renal Cell Carcinoma Progression
    Article Snippet: .. Indicated Taqman gene expression assays (Applied Biosystems) and the Taqman universal PCR master mix (Applied Biosystems) were used to quantify expression. .. Quantitative expression data were acquired and analyzed using an ABI Prism 7900HT Sequence Detection System (Applied Biosystems).

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: .. PCR was carried out in single-plex reactions in a 96-well optical plate with TaqMan Universal PCR Master Mix (Applied Biosystem) on ABI Prism 7700 Sequence Detection System (Applied Biosystems). ..

    Article Title: Temporal Analysis of Coxiella burnetii Morphological Differentiation
    Article Snippet: .. QPCR and reverse transcriptase PCR (RT-PCR) (see below) were performed using TaqMan Universal PCR Master Mix and a Prism 7000 sequence detection system (Applied Biosystems). .. Total RNA was purified from infected Vero cells cultivated in 25-cm2 tissue culture flasks.

    Article Title: Integrated analysis of microRNA and mRNA expression profiles in physiological myelopoiesis: role of hsa-mir-299-5p in CD34+ progenitor cells commitment
    Article Snippet: .. Hematopoietic lineage-specific messenger evaluation was assessed using TaqMan Gene Expression Assays and TaqMan Universal PCR Master Mix (Life Technologies-Applied Biosystems). ..

    Article Title: Dysregulation of miR-155-5p and miR-200-3p and the Anti-Non-Bilayer Phospholipid Arrangement Antibodies Favor the Development of Lupus in Three Novel Murine Lupus Models
    Article Snippet: .. The PCR amplification was run as described by the TaqMan Universal PCR Master Mix manual (Applied Biosystems) for the following differentially expressed miRNAs, as determined by PCR array analysis: miR-21a-5p, miR-125a-5p, miR-142-3p/5p, miR-146b/5p, miR-155-5p, and miR-200a-3p. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/?-catenin signaling pathway
    Article Snippet: .. The 100 ng cDNA products were used for quantitative real-time PCR performed using TaqMan Universal PCR Master Mix (Applied Biosystems) and TaqMan MGB primers [VEGFR-2 (Hs00911700_m1) and GAPDH (Hs99999905_m1)] with an ABI Prism 7500 (Applied Biosystems). ..

    Article Title: Cytomegalovirus pp71 Protein Is Expressed in Human Glioblastoma and Promotes Pro-Angiogenic Signaling by Activation of Stem Cell Factor
    Article Snippet: .. All assays were performed with Applied Biosystems TaqMan FAST Universal PCR Master Mix and the 7500 fast real-time PCR system. .. When necessary, standard curves were generated with serial dilutions of Ad169 viral DNA (Advanced Biotechnologies) or human genomic DNA (Applied Biosystems).

    Article Title: Temporal Analysis of Coxiella burnetii Morphological Differentiation
    Article Snippet: .. QPCR and reverse transcriptase PCR (RT-PCR) (see below) were performed using TaqMan Universal PCR Master Mix and a Prism 7000 sequence detection system (Applied Biosystems). .. Total RNA was purified from infected Vero cells cultivated in 25-cm2 tissue culture flasks.

    Sequencing:

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: .. PCR was carried out in single-plex reactions in a 96-well optical plate with TaqMan Universal PCR Master Mix (Applied Biosystem) on ABI Prism 7700 Sequence Detection System (Applied Biosystems). ..

    Article Title: Temporal Analysis of Coxiella burnetii Morphological Differentiation
    Article Snippet: .. QPCR and reverse transcriptase PCR (RT-PCR) (see below) were performed using TaqMan Universal PCR Master Mix and a Prism 7000 sequence detection system (Applied Biosystems). .. Total RNA was purified from infected Vero cells cultivated in 25-cm2 tissue culture flasks.

    Expressing:

    Article Title: Neutrophil Chemokines Secreted by Tumor Cells Mount a Lung Antimetastatic Response during Renal Cell Carcinoma Progression
    Article Snippet: .. Indicated Taqman gene expression assays (Applied Biosystems) and the Taqman universal PCR master mix (Applied Biosystems) were used to quantify expression. .. Quantitative expression data were acquired and analyzed using an ABI Prism 7900HT Sequence Detection System (Applied Biosystems).

    Article Title: Integrated analysis of microRNA and mRNA expression profiles in physiological myelopoiesis: role of hsa-mir-299-5p in CD34+ progenitor cells commitment
    Article Snippet: .. Hematopoietic lineage-specific messenger evaluation was assessed using TaqMan Gene Expression Assays and TaqMan Universal PCR Master Mix (Life Technologies-Applied Biosystems). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Temporal Analysis of Coxiella burnetii Morphological Differentiation
    Article Snippet: .. QPCR and reverse transcriptase PCR (RT-PCR) (see below) were performed using TaqMan Universal PCR Master Mix and a Prism 7000 sequence detection system (Applied Biosystems). .. Total RNA was purified from infected Vero cells cultivated in 25-cm2 tissue culture flasks.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher taqman universal master mix ii
    miR-182 Is Localized in RGC Axons (A) Heatmap representing the average expression of mature miRNAs from two axonal small <t>RNA-sequencing</t> (sRNA-seq) libraries prepared from stage 37/38 retinal cultures. The figure is sorted by decreasing axonal average values. (B) Fluorescent ISH on stage 35/36 RGC GCs cultured in vitro for 24 hr. (C) <t>TaqMan</t> qPCR performed on RNA extracted from laser-captured stage 37/38 RGC axons. U6 snRNA was used as positive control, because it is found in developing axons ( Natera-Naranjo et al., 2010 , Zhang et al., 2013 , Hancock et al., 2014 ). RT−, no template negative control; snRNAU6, U6 snRNA. Scale bar, 5 μm (B). See also Figure S1 and Table S1 .
    Taqman Universal Master Mix Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman universal master mix ii/product/Thermo Fisher
    Average 99 stars, based on 611 article reviews
    Price from $9.99 to $1999.99
    taqman universal master mix ii - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher universal pcr master mix
    The microRNA (miR)-96/182/183 cluster is upregulated in the livers of colesevelam-treated Zucker diabetic fatty (ZDF) rats. A : volcano plot depicting significantly altered miRNAs between colesevelam (Col) and vehicle-treated ZDF rat livers ( left ) and expression (reads per million total reads) of the miR-96/182/183 cluster ( left ); n = 6. B–D : liver expression of the miR-96/182/183 cluster by real-time <t>PCR;</t> n = 9–12. E : liver gene (mRNA) expression changes of Srebf2 and its target genes Hmgcr , Ldlr , and Sqle ; n = 8–12. For sequencing data, unpaired t -tests were used. For comparisons between 2 groups, Mann-Whitney nonparametric tests were used; n.d., not detectable.
    Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 589 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/universal pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 589 article reviews
    Price from $9.99 to $1999.99
    universal pcr master mix - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    miR-182 Is Localized in RGC Axons (A) Heatmap representing the average expression of mature miRNAs from two axonal small RNA-sequencing (sRNA-seq) libraries prepared from stage 37/38 retinal cultures. The figure is sorted by decreasing axonal average values. (B) Fluorescent ISH on stage 35/36 RGC GCs cultured in vitro for 24 hr. (C) TaqMan qPCR performed on RNA extracted from laser-captured stage 37/38 RGC axons. U6 snRNA was used as positive control, because it is found in developing axons ( Natera-Naranjo et al., 2010 , Zhang et al., 2013 , Hancock et al., 2014 ). RT−, no template negative control; snRNAU6, U6 snRNA. Scale bar, 5 μm (B). See also Figure S1 and Table S1 .

    Journal: Cell Reports

    Article Title: miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA

    doi: 10.1016/j.celrep.2016.12.093

    Figure Lengend Snippet: miR-182 Is Localized in RGC Axons (A) Heatmap representing the average expression of mature miRNAs from two axonal small RNA-sequencing (sRNA-seq) libraries prepared from stage 37/38 retinal cultures. The figure is sorted by decreasing axonal average values. (B) Fluorescent ISH on stage 35/36 RGC GCs cultured in vitro for 24 hr. (C) TaqMan qPCR performed on RNA extracted from laser-captured stage 37/38 RGC axons. U6 snRNA was used as positive control, because it is found in developing axons ( Natera-Naranjo et al., 2010 , Zhang et al., 2013 , Hancock et al., 2014 ). RT−, no template negative control; snRNAU6, U6 snRNA. Scale bar, 5 μm (B). See also Figure S1 and Table S1 .

    Article Snippet: The cDNA obtained was used for the TaqMan Micro RNA assay using xtr-miR-182-5p and U6 snRNA-specific primers and probes and the TaqMan Universal Master Mix II (MMIX II) no AmpErase Uracil N-Glycosylase (UNG) (all Thermo Fisher).

    Techniques: Expressing, RNA Sequencing Assay, In Situ Hybridization, Cell Culture, In Vitro, Real-time Polymerase Chain Reaction, Positive Control, Negative Control

    The microRNA (miR)-96/182/183 cluster is upregulated in the livers of colesevelam-treated Zucker diabetic fatty (ZDF) rats. A : volcano plot depicting significantly altered miRNAs between colesevelam (Col) and vehicle-treated ZDF rat livers ( left ) and expression (reads per million total reads) of the miR-96/182/183 cluster ( left ); n = 6. B–D : liver expression of the miR-96/182/183 cluster by real-time PCR; n = 9–12. E : liver gene (mRNA) expression changes of Srebf2 and its target genes Hmgcr , Ldlr , and Sqle ; n = 8–12. For sequencing data, unpaired t -tests were used. For comparisons between 2 groups, Mann-Whitney nonparametric tests were used; n.d., not detectable.

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Liver and Biliary Tract Physiology/Pathophysiology: Intestinal bile acid sequestration improves glucose control by stimulating hepatic miR-182-5p in type 2 diabetes

    doi: 10.1152/ajpgi.00238.2018

    Figure Lengend Snippet: The microRNA (miR)-96/182/183 cluster is upregulated in the livers of colesevelam-treated Zucker diabetic fatty (ZDF) rats. A : volcano plot depicting significantly altered miRNAs between colesevelam (Col) and vehicle-treated ZDF rat livers ( left ) and expression (reads per million total reads) of the miR-96/182/183 cluster ( left ); n = 6. B–D : liver expression of the miR-96/182/183 cluster by real-time PCR; n = 9–12. E : liver gene (mRNA) expression changes of Srebf2 and its target genes Hmgcr , Ldlr , and Sqle ; n = 8–12. For sequencing data, unpaired t -tests were used. For comparisons between 2 groups, Mann-Whitney nonparametric tests were used; n.d., not detectable.

    Article Snippet: Real-time PCR was completed with Universal PCR Master Mix and TaqMan mRNA and miRNA assays (ThermoFisher).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Sequencing, MANN-WHITNEY

    Mediator complex subunit 1 (Med1) is regulated by microRNA (miR)-96/182/183 in mice. A : expression of Med1 by real-time PCR in mouse primary hepatocytes transfected with 50 nM of Med1 or scramble siRNA; n = 3 B : Western blot of Med1 and β-actin (loading control) from protein samples of primary hepatocytes transfected with Med1 or scramble siRNA. Ratio of Med1/β-actin densitometry is indicated between the blots and graphed on the right ; n = 3. A.U., arbitrary units. C : hepatic Med1 mRNA levels in db/db mice treated with vehicle or 2% colesevelam (Col)-supplemented diet and PBS or locked-nucleic acid (LNA)-182-5p; real-time PCR; n = 8–9. D : hepatic Med1 and β-actin (loading control) protein levels from 3 representative liver protein samples for Chow + PBS, Col + PBS, and Col + LNA-182. Western blotting. Ratio of Med1/β-actin densitometry is indicated between the blots. E : densitometry quantification of Med1 protein levels normalized to β-actin; n = 8–9. F : expression of miR-96-5p, miR-182-5p, and miR-183-5p in mouse primary hepatocytes transfected with 50 nM miR-96-5p, miR-182-5p, miR-183-5p mimics individually or in combination; real-time PCR; n = 3. G : Med1 mRNA levels in mouse primary hepatocytes transfected with 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination; real-time PCR; n = 3. H : protein levels of Med1 and β-actin (loading control) from protein samples of primary hepatocytes transfected with mock or 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination. Ratio of Med1/β-actin densitometry is indicated between the blots and graphed on the right ; n = 3. For comparisons between 2 groups, Students’ t- tests were used, and for more than 2 groups, Kruskal-Wallis one-way ANOVA with Dunn’s posttest was used (α = 0.05) or one-way ANOVA with Bonferonni’s posttest for comparison to mock group only was used (α = 0.05).

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Liver and Biliary Tract Physiology/Pathophysiology: Intestinal bile acid sequestration improves glucose control by stimulating hepatic miR-182-5p in type 2 diabetes

    doi: 10.1152/ajpgi.00238.2018

    Figure Lengend Snippet: Mediator complex subunit 1 (Med1) is regulated by microRNA (miR)-96/182/183 in mice. A : expression of Med1 by real-time PCR in mouse primary hepatocytes transfected with 50 nM of Med1 or scramble siRNA; n = 3 B : Western blot of Med1 and β-actin (loading control) from protein samples of primary hepatocytes transfected with Med1 or scramble siRNA. Ratio of Med1/β-actin densitometry is indicated between the blots and graphed on the right ; n = 3. A.U., arbitrary units. C : hepatic Med1 mRNA levels in db/db mice treated with vehicle or 2% colesevelam (Col)-supplemented diet and PBS or locked-nucleic acid (LNA)-182-5p; real-time PCR; n = 8–9. D : hepatic Med1 and β-actin (loading control) protein levels from 3 representative liver protein samples for Chow + PBS, Col + PBS, and Col + LNA-182. Western blotting. Ratio of Med1/β-actin densitometry is indicated between the blots. E : densitometry quantification of Med1 protein levels normalized to β-actin; n = 8–9. F : expression of miR-96-5p, miR-182-5p, and miR-183-5p in mouse primary hepatocytes transfected with 50 nM miR-96-5p, miR-182-5p, miR-183-5p mimics individually or in combination; real-time PCR; n = 3. G : Med1 mRNA levels in mouse primary hepatocytes transfected with 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination; real-time PCR; n = 3. H : protein levels of Med1 and β-actin (loading control) from protein samples of primary hepatocytes transfected with mock or 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination. Ratio of Med1/β-actin densitometry is indicated between the blots and graphed on the right ; n = 3. For comparisons between 2 groups, Students’ t- tests were used, and for more than 2 groups, Kruskal-Wallis one-way ANOVA with Dunn’s posttest was used (α = 0.05) or one-way ANOVA with Bonferonni’s posttest for comparison to mock group only was used (α = 0.05).

    Article Snippet: Real-time PCR was completed with Universal PCR Master Mix and TaqMan mRNA and miRNA assays (ThermoFisher).

    Techniques: Mouse Assay, Expressing, Real-time Polymerase Chain Reaction, Transfection, Western Blot

    Mediator complex subunit 1 (MED1) is a direct target of microRNA (miR)-182-5p, miR-183-5p, and miR-96-5p in humans. A : schematic of human MED1 mRNA and the 3 putative miR-182/183/96 target sites in the 3′-untranslated region (3′-UTR). B : predicted MED1 3′-UTR target sites. Bases highlighted in dark gray represent mutations for gene reporter (luciferase) assays. C : normalized luciferase activity in HEK293 cells after dual transfection of miR-96/182/183 mimics and gene (luciferase) reporters harboring putative target sites for miR-96-5p, miR-182-5p, and/or miR-183-5p; n = 4. D : miR-96-5p, miR-182-5p, and miR-183-5p levels in Huh7 cells transfected with 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination; real-time PCR; n = 6. E : MED1 mRNA levels in Huh7 cells transfected with 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination; real-time PCR; n = 6. F : miR-96-5p, miR-182-5p, and miR-183-5p levels in Huh7 cells transfected with mock or 50 nM locked-nucleic acids (LNAs) against miR-182-5p and/or miR-183-5p; real-time PCR; n = 6. G : MED1 mRNA levels in Huh7 cells transfected mock or 50 nM LNAs against miR-182-5p and/or miR-183-5p; real-time PCR; n = 6. H : protein levels of Med1 and β-actin (loading control) from protein lysates of Huh7 cells transfected with mock or 50 nM miR-96, miR-182, and miR-183 LNAs. Ratio of Med1/β-actin densitometry is indicated between the blots and graphed on the right ; n = 6. A.U. arbitrary units. For comparisons between more than 2 groups to mock only, one-way ANOVA with Bonferonni’s posttest was used, α = 0.05. For comparisons between 2 groups, Mann-Whitney nonparametric tests were used.

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Liver and Biliary Tract Physiology/Pathophysiology: Intestinal bile acid sequestration improves glucose control by stimulating hepatic miR-182-5p in type 2 diabetes

    doi: 10.1152/ajpgi.00238.2018

    Figure Lengend Snippet: Mediator complex subunit 1 (MED1) is a direct target of microRNA (miR)-182-5p, miR-183-5p, and miR-96-5p in humans. A : schematic of human MED1 mRNA and the 3 putative miR-182/183/96 target sites in the 3′-untranslated region (3′-UTR). B : predicted MED1 3′-UTR target sites. Bases highlighted in dark gray represent mutations for gene reporter (luciferase) assays. C : normalized luciferase activity in HEK293 cells after dual transfection of miR-96/182/183 mimics and gene (luciferase) reporters harboring putative target sites for miR-96-5p, miR-182-5p, and/or miR-183-5p; n = 4. D : miR-96-5p, miR-182-5p, and miR-183-5p levels in Huh7 cells transfected with 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination; real-time PCR; n = 6. E : MED1 mRNA levels in Huh7 cells transfected with 50 nM miR-96-5p, miR-182-5p, and miR-183-5p mimics individually or in combination; real-time PCR; n = 6. F : miR-96-5p, miR-182-5p, and miR-183-5p levels in Huh7 cells transfected with mock or 50 nM locked-nucleic acids (LNAs) against miR-182-5p and/or miR-183-5p; real-time PCR; n = 6. G : MED1 mRNA levels in Huh7 cells transfected mock or 50 nM LNAs against miR-182-5p and/or miR-183-5p; real-time PCR; n = 6. H : protein levels of Med1 and β-actin (loading control) from protein lysates of Huh7 cells transfected with mock or 50 nM miR-96, miR-182, and miR-183 LNAs. Ratio of Med1/β-actin densitometry is indicated between the blots and graphed on the right ; n = 6. A.U. arbitrary units. For comparisons between more than 2 groups to mock only, one-way ANOVA with Bonferonni’s posttest was used, α = 0.05. For comparisons between 2 groups, Mann-Whitney nonparametric tests were used.

    Article Snippet: Real-time PCR was completed with Universal PCR Master Mix and TaqMan mRNA and miRNA assays (ThermoFisher).

    Techniques: Luciferase, Activity Assay, Transfection, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Quantitative real-time PCR analyses comparing miRNA expression patterns in tissues using different extraction methods. Fold abundance of miRNAs quantitated using TaqMan MicroRNA Assays via qRT-PCR from brain ( a ), liver ( b ) and lung ( c ) tissues. miRNA expression was normalised using sno202 as a reference gene. Values indicate the average normalised miRNA expression in different tissues on a Log2 scale with SEM error bars. * p

    Journal: BMC Biotechnology

    Article Title: Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal

    doi: 10.1186/s12896-018-0421-6

    Figure Lengend Snippet: Quantitative real-time PCR analyses comparing miRNA expression patterns in tissues using different extraction methods. Fold abundance of miRNAs quantitated using TaqMan MicroRNA Assays via qRT-PCR from brain ( a ), liver ( b ) and lung ( c ) tissues. miRNA expression was normalised using sno202 as a reference gene. Values indicate the average normalised miRNA expression in different tissues on a Log2 scale with SEM error bars. * p

    Article Snippet: Reverse transcription products were diluted to 50 μl and 5 μl of diluted sample used in single qPCR reactions, with a total volume of 20 μl. qRT-PCR was performed using Taqman microRNA assays (Thermo Fisher Scientific) and TaqMan universal PCR master mix II, no UNG (Thermo Fisher Scientific) on a ViiA7 Real-Time PCR system (Applied Biosystems/Thermo Fisher Scientific), using recommended PCR cycling conditions.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Quantitative RT-PCR

    Quantitative real-time PCR analyses of 6 miRNAs comparing different RNA extraction methods. RNA was extracted from murine brain ( a ), liver ( b ) and lung ( c ) tissues using the five indicated methods and endogenous miRNA expression quantitated using TaqMan MicroRNA Assays via qRT-PCR. miRNA expression was normalised using sno202 as a reference gene. Values indicate the average normalised miRNA expression (technical duplicates from triplicate biological isolations) in different tissues on a Log2 scale with SEM error bars. * p

    Journal: BMC Biotechnology

    Article Title: Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal

    doi: 10.1186/s12896-018-0421-6

    Figure Lengend Snippet: Quantitative real-time PCR analyses of 6 miRNAs comparing different RNA extraction methods. RNA was extracted from murine brain ( a ), liver ( b ) and lung ( c ) tissues using the five indicated methods and endogenous miRNA expression quantitated using TaqMan MicroRNA Assays via qRT-PCR. miRNA expression was normalised using sno202 as a reference gene. Values indicate the average normalised miRNA expression (technical duplicates from triplicate biological isolations) in different tissues on a Log2 scale with SEM error bars. * p

    Article Snippet: Reverse transcription products were diluted to 50 μl and 5 μl of diluted sample used in single qPCR reactions, with a total volume of 20 μl. qRT-PCR was performed using Taqman microRNA assays (Thermo Fisher Scientific) and TaqMan universal PCR master mix II, no UNG (Thermo Fisher Scientific) on a ViiA7 Real-Time PCR system (Applied Biosystems/Thermo Fisher Scientific), using recommended PCR cycling conditions.

    Techniques: Real-time Polymerase Chain Reaction, RNA Extraction, Expressing, Quantitative RT-PCR

    CKS1B and CKS2 expression is higher in diagnostic bone marrow samples from patients carrying MLL -translocations compared to PBMCs from healthy controls. RNA extracted from human umbilical cord blood CD34 + cells, PBMCs from healthy donors, and leukaemic patient samples, were assessed for (A) CKS1B and (B) CKS2 expression by TaqMan quantitative PCR. Three independent control genes were used ( ABL , GUS , B2M ) to measure relative RNA abundance. Values are represented as relative gene expression versus GUS control. Each point represents one patient. Median bars with standard deviation are presented for each set of samples. A Student's t -test was used to assess significance, and * indicates significance versus CD34 + cells, and § indicates significance versus PBMCs.

    Journal: Biochimica et Biophysica Acta

    Article Title: The Cks1/Cks2 axis fine-tunes Mll1 expression and is crucial for MLL-rearranged leukaemia cell viability

    doi: 10.1016/j.bbamcr.2017.09.009

    Figure Lengend Snippet: CKS1B and CKS2 expression is higher in diagnostic bone marrow samples from patients carrying MLL -translocations compared to PBMCs from healthy controls. RNA extracted from human umbilical cord blood CD34 + cells, PBMCs from healthy donors, and leukaemic patient samples, were assessed for (A) CKS1B and (B) CKS2 expression by TaqMan quantitative PCR. Three independent control genes were used ( ABL , GUS , B2M ) to measure relative RNA abundance. Values are represented as relative gene expression versus GUS control. Each point represents one patient. Median bars with standard deviation are presented for each set of samples. A Student's t -test was used to assess significance, and * indicates significance versus CD34 + cells, and § indicates significance versus PBMCs.

    Article Snippet: Quantitative PCR analyses were carried out using either the Quantitect SYBR Green RT-PCR kit (MEFs – Qiagen) or the TaqMan Universal PCR Master Mix kit (MLL -translocation cell lines – ThermoScientific).

    Techniques: Expressing, Diagnostic Assay, Real-time Polymerase Chain Reaction, Standard Deviation