taqman protein assays fast master mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher taqman protein assays fast master mix
    Effect of variant region deletion on 3D structure and gene expression (A) Combined 3C-qPCR data of chromatin interactions in dVR cells. Black: control; Blue: dVR. The numbering of NcoI DNA fragments is given relative to the viewpoint. Viewing point: R13 region; Black bars: STARD10 exon; red bars: regulatory region; orange box: qPCR probe; orange arrow: qPCR constant primer; green stars: credible set genetic variants. Data were normalized to a CXCL12 loading control. n = 3. (B) <t>Taqman</t> <t>qRT-PCR</t> analysis of gene expression in control and dVR cells. *, P
    Taqman Protein Assays Fast Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman protein assays fast master mix/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taqman protein assays fast master mix - by Bioz Stars, 2020-08
    84/100 stars

    Images

    1) Product Images from "Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a new regulator of insulin secretion"

    Article Title: Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a new regulator of insulin secretion

    Journal: bioRxiv

    doi: 10.1101/2020.03.31.017707

    Effect of variant region deletion on 3D structure and gene expression (A) Combined 3C-qPCR data of chromatin interactions in dVR cells. Black: control; Blue: dVR. The numbering of NcoI DNA fragments is given relative to the viewpoint. Viewing point: R13 region; Black bars: STARD10 exon; red bars: regulatory region; orange box: qPCR probe; orange arrow: qPCR constant primer; green stars: credible set genetic variants. Data were normalized to a CXCL12 loading control. n = 3. (B) Taqman qRT-PCR analysis of gene expression in control and dVR cells. *, P
    Figure Legend Snippet: Effect of variant region deletion on 3D structure and gene expression (A) Combined 3C-qPCR data of chromatin interactions in dVR cells. Black: control; Blue: dVR. The numbering of NcoI DNA fragments is given relative to the viewpoint. Viewing point: R13 region; Black bars: STARD10 exon; red bars: regulatory region; orange box: qPCR probe; orange arrow: qPCR constant primer; green stars: credible set genetic variants. Data were normalized to a CXCL12 loading control. n = 3. (B) Taqman qRT-PCR analysis of gene expression in control and dVR cells. *, P

    Techniques Used: Variant Assay, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Target genes regulated by the enhancer cluster. (A) Human islet promoter capture-HiC (pcHi-C) map at STARD10 locus and surrounding region. pcHi-C interactions were considered significant with CHiCAGO > 5. Orange interactions in the pcHiC track depict interactions mediated by the CTCF sites that flank the enhancer cluster (R1 and R13). All other significant interactions are shown in dark grey. Enhancer hubs track shows all enhancers (red) and promoters (green) contained within the STARD10 hub.Orange bar in islet TADs track highlights the TAD encompassing STARD10 and surrounding genes ( ARAP1, ATG16L2 , and FCHSD2 ). (B and C) Taqman qRT-PCR analysis of gene expression in sham control and R2-deleted (dR2) cells. *, P
    Figure Legend Snippet: Target genes regulated by the enhancer cluster. (A) Human islet promoter capture-HiC (pcHi-C) map at STARD10 locus and surrounding region. pcHi-C interactions were considered significant with CHiCAGO > 5. Orange interactions in the pcHiC track depict interactions mediated by the CTCF sites that flank the enhancer cluster (R1 and R13). All other significant interactions are shown in dark grey. Enhancer hubs track shows all enhancers (red) and promoters (green) contained within the STARD10 hub.Orange bar in islet TADs track highlights the TAD encompassing STARD10 and surrounding genes ( ARAP1, ATG16L2 , and FCHSD2 ). (B and C) Taqman qRT-PCR analysis of gene expression in sham control and R2-deleted (dR2) cells. *, P

    Techniques Used: Quantitative RT-PCR, Expressing

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Resolvin D-series regulates Phospholipase D both during inflammation and resolution by modulating phagocyte functions
    Article Snippet: .. Reverse transcription was performed in a 15 μl reaction volume with 1 μg of RNA, 1.5 μl 10T Buffer, 1 mM dNTPs, 3.8 units of RNase Inhibitor and 1 μl of Multiscribe Reverse Transcriptase and incubated in one-cycle at 16 °C for 30 min, 42 °C for 30 min and then 85 °C for 5 min. Quantitative PCR reactions were run in a 20 μl reaction volume using 10 μl Taqman Master Mix, ∼88 ng of total input RNA, 1 μl of the relevant micro-RNA gene expression assay (6-carboxyfluorescein (FAM)-labeled) multiplexed with U6 housekeeping gene. .. Taqman miR primers and fluorescent probes were from Life Technologies.

    Article Title: Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a new regulator of insulin secretion
    Article Snippet: .. Real-time PCR was performed on a 7500 Fast Real-Time PCR System using the Fast SYBR™ Green master mix or Fast Taqman™ master mix. .. The SYBR™ Green PCR primer sets for variant region and CTCF binding site (CBS) are listed in Table S6 and Table S9, respectively.

    SYBR Green Assay:

    Article Title: Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a new regulator of insulin secretion
    Article Snippet: .. Real-time PCR was performed on a 7500 Fast Real-Time PCR System using the Fast SYBR™ Green master mix or Fast Taqman™ master mix. .. The SYBR™ Green PCR primer sets for variant region and CTCF binding site (CBS) are listed in Table S6 and Table S9, respectively.

    Incubation:

    Article Title: Resolvin D-series regulates Phospholipase D both during inflammation and resolution by modulating phagocyte functions
    Article Snippet: .. Reverse transcription was performed in a 15 μl reaction volume with 1 μg of RNA, 1.5 μl 10T Buffer, 1 mM dNTPs, 3.8 units of RNase Inhibitor and 1 μl of Multiscribe Reverse Transcriptase and incubated in one-cycle at 16 °C for 30 min, 42 °C for 30 min and then 85 °C for 5 min. Quantitative PCR reactions were run in a 20 μl reaction volume using 10 μl Taqman Master Mix, ∼88 ng of total input RNA, 1 μl of the relevant micro-RNA gene expression assay (6-carboxyfluorescein (FAM)-labeled) multiplexed with U6 housekeeping gene. .. Taqman miR primers and fluorescent probes were from Life Technologies.

    other:

    Article Title: Regulation of heterotopic ossification through local inflammatory monocytes in a mouse model of aberrant wound healing
    Article Snippet: In 96-well plates, 5ul of diluted cDNA and 5ul of TaqMan master mix was added in a 1:1 ratio for total volume of 10 µLs.

    Expressing:

    Article Title: Resolvin D-series regulates Phospholipase D both during inflammation and resolution by modulating phagocyte functions
    Article Snippet: .. Reverse transcription was performed in a 15 μl reaction volume with 1 μg of RNA, 1.5 μl 10T Buffer, 1 mM dNTPs, 3.8 units of RNase Inhibitor and 1 μl of Multiscribe Reverse Transcriptase and incubated in one-cycle at 16 °C for 30 min, 42 °C for 30 min and then 85 °C for 5 min. Quantitative PCR reactions were run in a 20 μl reaction volume using 10 μl Taqman Master Mix, ∼88 ng of total input RNA, 1 μl of the relevant micro-RNA gene expression assay (6-carboxyfluorescein (FAM)-labeled) multiplexed with U6 housekeeping gene. .. Taqman miR primers and fluorescent probes were from Life Technologies.

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    Thermo Fisher taqman protein assays fast master mix
    Effect of variant region deletion on 3D structure and gene expression (A) Combined 3C-qPCR data of chromatin interactions in dVR cells. Black: control; Blue: dVR. The numbering of NcoI DNA fragments is given relative to the viewpoint. Viewing point: R13 region; Black bars: STARD10 exon; red bars: regulatory region; orange box: qPCR probe; orange arrow: qPCR constant primer; green stars: credible set genetic variants. Data were normalized to a CXCL12 loading control. n = 3. (B) <t>Taqman</t> <t>qRT-PCR</t> analysis of gene expression in control and dVR cells. *, P
    Taqman Protein Assays Fast Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman protein assays fast master mix/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taqman protein assays fast master mix - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

    92
    Thermo Fisher taqman protein assays fast master mix 2x
    Effect of variant region deletion on 3D structure and gene expression (A) Combined 3C-qPCR data of chromatin interactions in dVR cells. Black: control; Blue: dVR. The numbering of NcoI DNA fragments is given relative to the viewpoint. Viewing point: R13 region; Black bars: STARD10 exon; red bars: regulatory region; orange box: qPCR probe; orange arrow: qPCR constant primer; green stars: credible set genetic variants. Data were normalized to a CXCL12 loading control. n = 3. (B) <t>Taqman</t> <t>qRT-PCR</t> analysis of gene expression in control and dVR cells. *, P
    Taqman Protein Assays Fast Master Mix 2x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman protein assays fast master mix 2x/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taqman protein assays fast master mix 2x - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effect of variant region deletion on 3D structure and gene expression (A) Combined 3C-qPCR data of chromatin interactions in dVR cells. Black: control; Blue: dVR. The numbering of NcoI DNA fragments is given relative to the viewpoint. Viewing point: R13 region; Black bars: STARD10 exon; red bars: regulatory region; orange box: qPCR probe; orange arrow: qPCR constant primer; green stars: credible set genetic variants. Data were normalized to a CXCL12 loading control. n = 3. (B) Taqman qRT-PCR analysis of gene expression in control and dVR cells. *, P

    Journal: bioRxiv

    Article Title: Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a new regulator of insulin secretion

    doi: 10.1101/2020.03.31.017707

    Figure Lengend Snippet: Effect of variant region deletion on 3D structure and gene expression (A) Combined 3C-qPCR data of chromatin interactions in dVR cells. Black: control; Blue: dVR. The numbering of NcoI DNA fragments is given relative to the viewpoint. Viewing point: R13 region; Black bars: STARD10 exon; red bars: regulatory region; orange box: qPCR probe; orange arrow: qPCR constant primer; green stars: credible set genetic variants. Data were normalized to a CXCL12 loading control. n = 3. (B) Taqman qRT-PCR analysis of gene expression in control and dVR cells. *, P

    Article Snippet: Real-time PCR was performed on a 7500 Fast Real-Time PCR System using the Fast SYBR™ Green master mix or Fast Taqman™ master mix.

    Techniques: Variant Assay, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Target genes regulated by the enhancer cluster. (A) Human islet promoter capture-HiC (pcHi-C) map at STARD10 locus and surrounding region. pcHi-C interactions were considered significant with CHiCAGO > 5. Orange interactions in the pcHiC track depict interactions mediated by the CTCF sites that flank the enhancer cluster (R1 and R13). All other significant interactions are shown in dark grey. Enhancer hubs track shows all enhancers (red) and promoters (green) contained within the STARD10 hub.Orange bar in islet TADs track highlights the TAD encompassing STARD10 and surrounding genes ( ARAP1, ATG16L2 , and FCHSD2 ). (B and C) Taqman qRT-PCR analysis of gene expression in sham control and R2-deleted (dR2) cells. *, P

    Journal: bioRxiv

    Article Title: Chromatin 3D interaction analysis of the STARD10 locus unveils FCHSD2 as a new regulator of insulin secretion

    doi: 10.1101/2020.03.31.017707

    Figure Lengend Snippet: Target genes regulated by the enhancer cluster. (A) Human islet promoter capture-HiC (pcHi-C) map at STARD10 locus and surrounding region. pcHi-C interactions were considered significant with CHiCAGO > 5. Orange interactions in the pcHiC track depict interactions mediated by the CTCF sites that flank the enhancer cluster (R1 and R13). All other significant interactions are shown in dark grey. Enhancer hubs track shows all enhancers (red) and promoters (green) contained within the STARD10 hub.Orange bar in islet TADs track highlights the TAD encompassing STARD10 and surrounding genes ( ARAP1, ATG16L2 , and FCHSD2 ). (B and C) Taqman qRT-PCR analysis of gene expression in sham control and R2-deleted (dR2) cells. *, P

    Article Snippet: Real-time PCR was performed on a 7500 Fast Real-Time PCR System using the Fast SYBR™ Green master mix or Fast Taqman™ master mix.

    Techniques: Quantitative RT-PCR, Expressing