taqman preamp master mix kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher taqman preamp master mix kit
    Taqman Preamp Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman preamp master mix kit/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    taqman preamp master mix kit - by Bioz Stars, 2020-01
    99/100 stars

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    Serial Dilution:

    Article Title: The integrative knowledge base for miRNA-mRNA expression in colorectal cancer
    Article Snippet: For mRNAs quantification, the resulting cDNA was pre-amplified using a TaqMan PreAmp Master Mix Kit (Thermo Fisher Scientific). .. The expression levels were calculated after efficiency correction (based on the results of serial dilution) relative to four endogenous controls hsa- miR-1247b and RNU6B for miRNAs and IPO8 and B2M for mRNAs.

    Quantitative RT-PCR:

    Article Title: Comparative transcriptomics between species attributes reactogenicity pathways induced by the capsular group B meningococcal vaccine, 4CMenB, to the membrane-bound endotoxin of its outer membrane vesicle component
    Article Snippet: Real time quantitative PCR BEC RNA was reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher) and pre-amplified using pooled TaqMan™ primers (Supplementary Table ) at a concentration of 0.4X and a TaqMan™ PreAmp Master Mix Kit (ThermoFisher). .. Samples were assayed in triplicate for each primer and RT-qPCR was performed using a StepOnePlus™ Real-Time PCR System (ThermoFisher).

    Real-time Polymerase Chain Reaction:

    Article Title: Comparative transcriptomics between species attributes reactogenicity pathways induced by the capsular group B meningococcal vaccine, 4CMenB, to the membrane-bound endotoxin of its outer membrane vesicle component
    Article Snippet: .. Real time quantitative PCR BEC RNA was reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher) and pre-amplified using pooled TaqMan™ primers (Supplementary Table ) at a concentration of 0.4X and a TaqMan™ PreAmp Master Mix Kit (ThermoFisher). .. Pre-amplified cDNA samples were diluted 1:20 in 1X TE buffer (Invitrogen) and combined in the appropriate ratio with TaqMan™ Gene Expression Master Mix (ThermoFisher), nuclease-free water, and the appropriate primer on a MicroAmp Fast Optical 96-Well Reaction Plate (0.1, ThermoFisher).

    Article Title: The integrative knowledge base for miRNA-mRNA expression in colorectal cancer
    Article Snippet: Paragraph title: Reverse transcription and qPCR experiment ... For mRNAs quantification, the resulting cDNA was pre-amplified using a TaqMan PreAmp Master Mix Kit (Thermo Fisher Scientific).

    Concentration Assay:

    Article Title: Comparative transcriptomics between species attributes reactogenicity pathways induced by the capsular group B meningococcal vaccine, 4CMenB, to the membrane-bound endotoxin of its outer membrane vesicle component
    Article Snippet: .. Real time quantitative PCR BEC RNA was reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher) and pre-amplified using pooled TaqMan™ primers (Supplementary Table ) at a concentration of 0.4X and a TaqMan™ PreAmp Master Mix Kit (ThermoFisher). .. Pre-amplified cDNA samples were diluted 1:20 in 1X TE buffer (Invitrogen) and combined in the appropriate ratio with TaqMan™ Gene Expression Master Mix (ThermoFisher), nuclease-free water, and the appropriate primer on a MicroAmp Fast Optical 96-Well Reaction Plate (0.1, ThermoFisher).

    Expressing:

    Article Title: Comparative transcriptomics between species attributes reactogenicity pathways induced by the capsular group B meningococcal vaccine, 4CMenB, to the membrane-bound endotoxin of its outer membrane vesicle component
    Article Snippet: Real time quantitative PCR BEC RNA was reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher) and pre-amplified using pooled TaqMan™ primers (Supplementary Table ) at a concentration of 0.4X and a TaqMan™ PreAmp Master Mix Kit (ThermoFisher). .. Pre-amplified cDNA samples were diluted 1:20 in 1X TE buffer (Invitrogen) and combined in the appropriate ratio with TaqMan™ Gene Expression Master Mix (ThermoFisher), nuclease-free water, and the appropriate primer on a MicroAmp Fast Optical 96-Well Reaction Plate (0.1, ThermoFisher).

    Article Title: The integrative knowledge base for miRNA-mRNA expression in colorectal cancer
    Article Snippet: Reverse transcription and qPCR experiment Gene expression levels were determined using TaqMan based qPCR (Thermo Fisher Scientific), which was performed on the RotorGene system (Qiagen). .. For mRNAs quantification, the resulting cDNA was pre-amplified using a TaqMan PreAmp Master Mix Kit (Thermo Fisher Scientific).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The integrative knowledge base for miRNA-mRNA expression in colorectal cancer
    Article Snippet: Total RNA was reverse transcribed, 60 ng using the One Taq RT PCR Kit (NEB) according to the manufacturer’s instructions for mRNA quantification and 10 ng using the TaqMan microRNA reverse transcription kit (Thermo Fisher Scientific) according to the manufacturer’s instructions for miRNA quantification. .. For mRNAs quantification, the resulting cDNA was pre-amplified using a TaqMan PreAmp Master Mix Kit (Thermo Fisher Scientific).

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  • 90
    Thermo Fisher preamp master kit
    Preamp Master Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preamp master kit/product/Thermo Fisher
    Average 90 stars, based on 399 article reviews
    Price from $9.99 to $1999.99
    preamp master kit - by Bioz Stars, 2020-01
    90/100 stars
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