taqman gene expression master mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher taqman gene expression master mix
    Taqman Gene Expression Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman gene expression master mix/product/Thermo Fisher
    Average 99 stars, based on 2269 article reviews
    Price from $9.99 to $1999.99
    taqman gene expression master mix - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Mulberry leaf reduces inflammation and insulin resistance in type 2 diabetic mice by TLRs and insulin Signalling pathway
    Article Snippet: .. The cDNA was amplified utilizing TaqMan Gene expression Master Mix (Applied Biosystems, USA) on CFX96. .. The primers were directly purchased from Applied Biosystems: Insulin Receptor (InsR) (FAM, Mm01211875-ml), Insulin Receptor Substrate 1 (IRS1) (FAM, Mm01278327-ml), β-actin (VIC PL, Mm00607939-S1).

    Synthesized:

    Article Title: Calcipotriol and betamethasone dipropionate synergistically enhances the balance between regulatory and proinflammatory T cells in a murine psoriasis model
    Article Snippet: .. RNA was obtained from ear skin samples with an RNeasy Fibrous Tissue Mini Kit (QIAGEN), and complementary DNA (cDNA) was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen Japan, Tokyo, Japan). cDNA, probes, and TaqMan Gene Expression Master Mix (Applied Biosystems, Tokyo, Japan) were then mixed in a 96-well PCR plate, and quantitative PCR was performed using an ABI StepOnePlusTM Real-Time PCR System (Applied Biosystems). .. The following Taqman Gene Expression Assay probes were used in this study: Gapdh: Mm99999915_g1, Il17a : Mm00439619_m1, IL22 : Mm00444241_m1, Il23a : m00518984_m1 and Tnf : MM00443258_m1.

    Article Title: Mitochondrial Complex I Deficiency in Schizophrenia and Bipolar Disorder and Medication Influence
    Article Snippet: A 301-bp gBlock Gene Fragment (Integrated DNA Technologies, Coralville, IA, USA) was synthesized encompassing the mitochondria common deletion breakpoints (mt8224-8469 and mt13447-13501). .. The 12 µL total reaction volume consisted of: 0.9 µ M each primer (deletion forward 5′-AGGGCCCGTATTTACCCTAT-3′ and deletion reverse 5′-GCTAATGCTAGGCTGCCAAT-3′), 0.25 µ M TaqMan Probe 5′-6-FAM-CTAAGTTAGCTTTACAGTGGGCTCTAGAGG-MGBNFQ-3′, and 6 µL TaqMan Gene Expression Master Mix (Applied Biosystems).

    Article Title: Mulberry leaf reduces inflammation and insulin resistance in type 2 diabetic mice by TLRs and insulin Signalling pathway
    Article Snippet: The thermal cycling conditions started with Hold step at 95 °C for 10 min, followed by 40 cycles with denaturing at 95 °C for 15 s, and extended at 60 °C for 60 s. Total RNA was extracted from adipose tissue, and cDNA was synthesized by 1 μg as described above. .. The cDNA was amplified utilizing TaqMan Gene expression Master Mix (Applied Biosystems, USA) on CFX96.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Selected mRNAs were measured using mRNA targets TaqMan® Assays (Applied Biosystems). cDNAs were synthesized using the high capacity cDNA reverse transcription kit (Life Technologies Corporation, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. TaqMan® Gene Expression Master Mix (Applied Biosystems) was mixed together with probes and primers for the selected targets (Sp4 Transcription Factor (SP4 ): Hs00162095_m1; Basic Helix-Loop-Helix Family Member E40 (BHLHE40 ): Hs00186419_m1; Calpain Small Subunit 1 (CAPNS1 ): Hs00998426_m1; Ras Homolog Family Member A (RHOA ): Hs01051295_m1; Regulator Of G Protein Signaling 16 (RGS16 ): Hs00892674_m1; Activator Of Transcription And Developmental Regulator AUTS2 (AUTS2 ): Hs01688766_m1; Kynurenine Aminotransferase 1 (KYAT1 ): Hs00187858).

    Quantitative RT-PCR:

    Article Title: Metabolic Activation of Flavin Monooxygenase-mediated Trimethylamine-N-Oxide Formation in Experimental Kidney Disease
    Article Snippet: .. Fmo3 , Fmo1 , Arnt , Ahr , Cyp1a2 , Cyp3a2 and Actb (β-Actin) genes were quantified by RT-qPCR with Applied Biosystems 7500 Fast Real-Time PCR System (Foster City, CA) using Taqman® Gene Expression Master Mix (Applied Biosystems, Foster City, CA) and Taqman® specific primers. ..

    Article Title: Structural analysis identifies an escape route from the adverse lipogenic effects of liver X receptor ligands
    Article Snippet: In the study with BMS-852907, RT-qPCR reactions were carried out in separate triplicate wells for Abca1 and Rplp0, respectively, by using 20 ng of cDNA template, 385 nM of the above-mentioned primers, and iQ™ SYBR Green Supermix (Bio-Rad, Hercules, California, USA) on a Bio-Rad MyiQ instrument (Bio-Rad, Hercules, California, USA). .. In the other studies, 400 nM Abca1 forward and reverse primers, 200 nM Abca1 probe, 200 nM Rplp0 forward and reverse primers, and 100 nM Rplp0 probe were mixed with 15 ng of cDNA template and TaqMan™ Gene Expression Master Mix (Applied Biosystems, Foster City, USA).

    Article Title: Mulberry leaf reduces inflammation and insulin resistance in type 2 diabetic mice by TLRs and insulin Signalling pathway
    Article Snippet: Paragraph title: Quantitative real-time reverse transcript-polymerase chain reaction (RT-qPCR) analysis ... The cDNA was amplified utilizing TaqMan Gene expression Master Mix (Applied Biosystems, USA) on CFX96.

    Article Title: Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming
    Article Snippet: For the TaqMan QRT-PCR panel, cDNA mixture of three parallel samples was used in case of each condition: control, 6, 24, and 72 h exosome treatment applied on MSC cells derived from four mice. .. Eighty nanogram cDNA and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) were used for the qPCR experiment.

    Article Title: Molecular Profiling Reveals Unique Immune and Metabolic Features of Melanoma Brain Metastases
    Article Snippet: .. All qRT-PCR reactions were performed using the 7900HT Fast Real-Time PCR system and Taqman gene expression master mix (Applied Biosystems) with a standard cycling program of 40 cycles at 95 °C for 15 s and at 60 °C for 1 min. All reactions were run in triplicate and normalized to human 18S (Hs99999901_s1). ..

    Article Title: The bone marrow microenvironment at single-cell resolution
    Article Snippet: Paragraph title: qRT-PCR. ... Real time PCR reactions were carried out using TaqMan Gene Expression Master Mix (Life Technologies) and run on a Lightcycler 480 II (Roche).

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Paragraph title: 4.5. Validation with Quantitative Reverse Transcription-PCR (qRT-PCR) ... TaqMan® Gene Expression Master Mix (Applied Biosystems) was mixed together with probes and primers for the selected targets (Sp4 Transcription Factor (SP4 ): Hs00162095_m1; Basic Helix-Loop-Helix Family Member E40 (BHLHE40 ): Hs00186419_m1; Calpain Small Subunit 1 (CAPNS1 ): Hs00998426_m1; Ras Homolog Family Member A (RHOA ): Hs01051295_m1; Regulator Of G Protein Signaling 16 (RGS16 ): Hs00892674_m1; Activator Of Transcription And Developmental Regulator AUTS2 (AUTS2 ): Hs01688766_m1; Kynurenine Aminotransferase 1 (KYAT1 ): Hs00187858).

    Real-time Polymerase Chain Reaction:

    Article Title: Metabolic Activation of Flavin Monooxygenase-mediated Trimethylamine-N-Oxide Formation in Experimental Kidney Disease
    Article Snippet: .. Fmo3 , Fmo1 , Arnt , Ahr , Cyp1a2 , Cyp3a2 and Actb (β-Actin) genes were quantified by RT-qPCR with Applied Biosystems 7500 Fast Real-Time PCR System (Foster City, CA) using Taqman® Gene Expression Master Mix (Applied Biosystems, Foster City, CA) and Taqman® specific primers. ..

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: Paragraph title: High-throughput qPCR using the 96.96 Dynamic Array ... Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA.

    Article Title: A phase Ib study of capecitabine and ziv-aflibercept followed by a phase II single-arm expansion cohort in chemotherapy refractory metastatic colorectal cancer
    Article Snippet: .. Expression levels of VEGF-A, VEGF-C, VEGF-D, PlGF, NRP1, and NRP2 were quantified using preformulated TaqMan real-time PCR assays and TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA) in 10 μl reactions according to the manufacturer’s instructions. .. If the standard deviation (SD) in cycle thresholds (CT) between replicate wells was > 0.5 then the reaction was rerun.

    Article Title: Calcipotriol and betamethasone dipropionate synergistically enhances the balance between regulatory and proinflammatory T cells in a murine psoriasis model
    Article Snippet: .. RNA was obtained from ear skin samples with an RNeasy Fibrous Tissue Mini Kit (QIAGEN), and complementary DNA (cDNA) was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen Japan, Tokyo, Japan). cDNA, probes, and TaqMan Gene Expression Master Mix (Applied Biosystems, Tokyo, Japan) were then mixed in a 96-well PCR plate, and quantitative PCR was performed using an ABI StepOnePlusTM Real-Time PCR System (Applied Biosystems). .. The following Taqman Gene Expression Assay probes were used in this study: Gapdh: Mm99999915_g1, Il17a : Mm00439619_m1, IL22 : Mm00444241_m1, Il23a : m00518984_m1 and Tnf : MM00443258_m1.

    Article Title: Microbiota analysis optimization for human bronchoalveolar lavage fluid
    Article Snippet: .. 16S qPCR primers and conditions are described in Additional file . qPCR reactions were carried out in a volume of 11 μl using the TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol. .. Concentration Samples were concentrated using Amicon Ultra-15 Centrifugal Filter Units with the 30-kDa filter (MilliporeSigma, Darmstadt, Germany).

    Article Title: Sitagliptin-Dependent Differences in the Intensity of Oxidative Stress in Rat Livers Subjected to Ischemia and Reperfusion
    Article Snippet: .. Real-Time PCR The mRNA expression of SOD1 was determined by real-time PCR with 7900HT Fast Real-Time PCR System and TaqMan Gene Expression Master Mix (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Structural analysis identifies an escape route from the adverse lipogenic effects of liver X receptor ligands
    Article Snippet: Paragraph title: RNA preparation and qPCR ... In the other studies, 400 nM Abca1 forward and reverse primers, 200 nM Abca1 probe, 200 nM Rplp0 forward and reverse primers, and 100 nM Rplp0 probe were mixed with 15 ng of cDNA template and TaqMan™ Gene Expression Master Mix (Applied Biosystems, Foster City, USA).

    Article Title: Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming
    Article Snippet: .. Eighty nanogram cDNA and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) were used for the qPCR experiment. .. Expression of the examined 44 genes was calculated by ΔΔCt method and normalized to the average Ct values of 4 internal controls (PPIA, 18S RNA, ACTB, and GAPDH).

    Article Title: Molecular Profiling Reveals Unique Immune and Metabolic Features of Melanoma Brain Metastases
    Article Snippet: .. All qRT-PCR reactions were performed using the 7900HT Fast Real-Time PCR system and Taqman gene expression master mix (Applied Biosystems) with a standard cycling program of 40 cycles at 95 °C for 15 s and at 60 °C for 1 min. All reactions were run in triplicate and normalized to human 18S (Hs99999901_s1). ..

    Article Title: The bone marrow microenvironment at single-cell resolution
    Article Snippet: .. Real time PCR reactions were carried out using TaqMan Gene Expression Master Mix (Life Technologies) and run on a Lightcycler 480 II (Roche). .. The following genes were analysed with TaqMan Gene Expression Assays: Gapdh (Mm99999915_g1), Dll4 (Mm00444619), Dll1 (Mm01279269_m1), Jag1 (Mm00496902_m1), Cdh5 (Mm00486938_m1), Lepr (Mm00440181_m1), Col1a1 (Mm00801666_g1).

    Article Title: High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats
    Article Snippet: The quantity of RNA and cDNA was measured with the Thermo Scientific μDrop Plate using spectrophotometer (Multiskan GO; Thermo Fisher Scientific Corporation, Vantaa, Finland). cDNA samples were stored at −20 °C until real-time PCR analysis. .. The PCR reaction mixture contained cDNA samples, RNase-free water, TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, California, USA), and primers for following genes: Fatty acid synthase (Fasn ), acetyl-CoA carboxylase (Acaca ), stearoyl-CoA desaturase 1 (Scd1 ), MLX-interacting protein-like (Mlxipl) (known also as Carbohydrate-responsive element-binding protein (ChREBP )), and additionally in the liver on factors: Factor sterol regulatory element binding protein 1 (Srebf1 ), carnitine palmitoyltransferase 1a (Cpt1a ), and peroxisome proliferator-activated receptor alpha (Ppar-α ) with fluorescent marked starters.

    Article Title: Impact of peripheral immune status on central molecular responses to facial nerve axotomy
    Article Snippet: Paragraph title: 2.5. qPCR ... The 20 μl reaction volume contained 1 μl of cDNA, 1 μl of 20× TaqMan® FAM gene expression assay , 8 μl of 0.002% diethyl pyrocarbonate-treated water, and 10 μl of TaqMan Gene Expression Master Mix (Thermo Fisher Scientific; 4369016).

    Incubation:

    Article Title: High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats
    Article Snippet: The PCR reaction mixture contained cDNA samples, RNase-free water, TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, California, USA), and primers for following genes: Fatty acid synthase (Fasn ), acetyl-CoA carboxylase (Acaca ), stearoyl-CoA desaturase 1 (Scd1 ), MLX-interacting protein-like (Mlxipl) (known also as Carbohydrate-responsive element-binding protein (ChREBP )), and additionally in the liver on factors: Factor sterol regulatory element binding protein 1 (Srebf1 ), carnitine palmitoyltransferase 1a (Cpt1a ), and peroxisome proliferator-activated receptor alpha (Ppar-α ) with fluorescent marked starters. .. The reaction mixture was incubated for 2 min at 50 °C and for 10 min at 95 °C (initial step), as well as 40 cycles of 95 °C for 15 s (denaturation) and 60 °C for 1 min (annealing/extension) using CFX96 Touch™ Deep Well Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

    Standard Deviation:

    Article Title: A phase Ib study of capecitabine and ziv-aflibercept followed by a phase II single-arm expansion cohort in chemotherapy refractory metastatic colorectal cancer
    Article Snippet: Expression levels of VEGF-A, VEGF-C, VEGF-D, PlGF, NRP1, and NRP2 were quantified using preformulated TaqMan real-time PCR assays and TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA) in 10 μl reactions according to the manufacturer’s instructions. .. If the standard deviation (SD) in cycle thresholds (CT) between replicate wells was > 0.5 then the reaction was rerun.

    Expressing:

    Article Title: Metabolic Activation of Flavin Monooxygenase-mediated Trimethylamine-N-Oxide Formation in Experimental Kidney Disease
    Article Snippet: .. Fmo3 , Fmo1 , Arnt , Ahr , Cyp1a2 , Cyp3a2 and Actb (β-Actin) genes were quantified by RT-qPCR with Applied Biosystems 7500 Fast Real-Time PCR System (Foster City, CA) using Taqman® Gene Expression Master Mix (Applied Biosystems, Foster City, CA) and Taqman® specific primers. ..

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: .. Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. .. Thermal cycling conditions for qPCR were: thermal mix 50°C for 2 min, 70°C for 30 min, 25°C for 10 min, followed by hot start 50°C for 2 min, 95°C for 10 min, PCR (x30 cycles) 95°C for 15 sec, 60°C for 60 sec and melting curve analysis 60°C for 3 sec to 95°C.

    Article Title: A phase Ib study of capecitabine and ziv-aflibercept followed by a phase II single-arm expansion cohort in chemotherapy refractory metastatic colorectal cancer
    Article Snippet: .. Expression levels of VEGF-A, VEGF-C, VEGF-D, PlGF, NRP1, and NRP2 were quantified using preformulated TaqMan real-time PCR assays and TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA) in 10 μl reactions according to the manufacturer’s instructions. .. If the standard deviation (SD) in cycle thresholds (CT) between replicate wells was > 0.5 then the reaction was rerun.

    Article Title: Calcipotriol and betamethasone dipropionate synergistically enhances the balance between regulatory and proinflammatory T cells in a murine psoriasis model
    Article Snippet: .. RNA was obtained from ear skin samples with an RNeasy Fibrous Tissue Mini Kit (QIAGEN), and complementary DNA (cDNA) was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen Japan, Tokyo, Japan). cDNA, probes, and TaqMan Gene Expression Master Mix (Applied Biosystems, Tokyo, Japan) were then mixed in a 96-well PCR plate, and quantitative PCR was performed using an ABI StepOnePlusTM Real-Time PCR System (Applied Biosystems). .. The following Taqman Gene Expression Assay probes were used in this study: Gapdh: Mm99999915_g1, Il17a : Mm00439619_m1, IL22 : Mm00444241_m1, Il23a : m00518984_m1 and Tnf : MM00443258_m1.

    Article Title: Microbiota analysis optimization for human bronchoalveolar lavage fluid
    Article Snippet: .. 16S qPCR primers and conditions are described in Additional file . qPCR reactions were carried out in a volume of 11 μl using the TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol. .. Concentration Samples were concentrated using Amicon Ultra-15 Centrifugal Filter Units with the 30-kDa filter (MilliporeSigma, Darmstadt, Germany).

    Article Title: Mitochondrial Complex I Deficiency in Schizophrenia and Bipolar Disorder and Medication Influence
    Article Snippet: .. The 12 µL total reaction volume consisted of: 0.9 µ M each primer (deletion forward 5′-AGGGCCCGTATTTACCCTAT-3′ and deletion reverse 5′-GCTAATGCTAGGCTGCCAAT-3′), 0.25 µ M TaqMan Probe 5′-6-FAM-CTAAGTTAGCTTTACAGTGGGCTCTAGAGG-MGBNFQ-3′, and 6 µL TaqMan Gene Expression Master Mix (Applied Biosystems). .. Cycling parameters were 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min on the Applied Biosystems 7900HT real-time machine, selecting ROX as the passive reference.

    Article Title: Sitagliptin-Dependent Differences in the Intensity of Oxidative Stress in Rat Livers Subjected to Ischemia and Reperfusion
    Article Snippet: .. Real-Time PCR The mRNA expression of SOD1 was determined by real-time PCR with 7900HT Fast Real-Time PCR System and TaqMan Gene Expression Master Mix (Applied Biosystems, Carlsbad, CA, USA). ..

    Article Title: Structural analysis identifies an escape route from the adverse lipogenic effects of liver X receptor ligands
    Article Snippet: .. In the other studies, 400 nM Abca1 forward and reverse primers, 200 nM Abca1 probe, 200 nM Rplp0 forward and reverse primers, and 100 nM Rplp0 probe were mixed with 15 ng of cDNA template and TaqMan™ Gene Expression Master Mix (Applied Biosystems, Foster City, USA). .. The RT-qPCR reactions were performed on a 7500 Real-time PCR system (Applied Biosystems, Foster City, USA).

    Article Title: Mulberry leaf reduces inflammation and insulin resistance in type 2 diabetic mice by TLRs and insulin Signalling pathway
    Article Snippet: .. The cDNA was amplified utilizing TaqMan Gene expression Master Mix (Applied Biosystems, USA) on CFX96. .. The primers were directly purchased from Applied Biosystems: Insulin Receptor (InsR) (FAM, Mm01211875-ml), Insulin Receptor Substrate 1 (IRS1) (FAM, Mm01278327-ml), β-actin (VIC PL, Mm00607939-S1).

    Article Title: Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming
    Article Snippet: .. Eighty nanogram cDNA and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) were used for the qPCR experiment. .. Expression of the examined 44 genes was calculated by ΔΔCt method and normalized to the average Ct values of 4 internal controls (PPIA, 18S RNA, ACTB, and GAPDH).

    Article Title: Molecular Profiling Reveals Unique Immune and Metabolic Features of Melanoma Brain Metastases
    Article Snippet: .. All qRT-PCR reactions were performed using the 7900HT Fast Real-Time PCR system and Taqman gene expression master mix (Applied Biosystems) with a standard cycling program of 40 cycles at 95 °C for 15 s and at 60 °C for 1 min. All reactions were run in triplicate and normalized to human 18S (Hs99999901_s1). ..

    Article Title: The bone marrow microenvironment at single-cell resolution
    Article Snippet: .. Real time PCR reactions were carried out using TaqMan Gene Expression Master Mix (Life Technologies) and run on a Lightcycler 480 II (Roche). .. The following genes were analysed with TaqMan Gene Expression Assays: Gapdh (Mm99999915_g1), Dll4 (Mm00444619), Dll1 (Mm01279269_m1), Jag1 (Mm00496902_m1), Cdh5 (Mm00486938_m1), Lepr (Mm00440181_m1), Col1a1 (Mm00801666_g1).

    Article Title: High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats
    Article Snippet: .. The PCR reaction mixture contained cDNA samples, RNase-free water, TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, California, USA), and primers for following genes: Fatty acid synthase (Fasn ), acetyl-CoA carboxylase (Acaca ), stearoyl-CoA desaturase 1 (Scd1 ), MLX-interacting protein-like (Mlxipl) (known also as Carbohydrate-responsive element-binding protein (ChREBP )), and additionally in the liver on factors: Factor sterol regulatory element binding protein 1 (Srebf1 ), carnitine palmitoyltransferase 1a (Cpt1a ), and peroxisome proliferator-activated receptor alpha (Ppar-α ) with fluorescent marked starters. .. The reaction mixture was incubated for 2 min at 50 °C and for 10 min at 95 °C (initial step), as well as 40 cycles of 95 °C for 15 s (denaturation) and 60 °C for 1 min (annealing/extension) using CFX96 Touch™ Deep Well Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

    Article Title: Impact of peripheral immune status on central molecular responses to facial nerve axotomy
    Article Snippet: .. The 20 μl reaction volume contained 1 μl of cDNA, 1 μl of 20× TaqMan® FAM gene expression assay , 8 μl of 0.002% diethyl pyrocarbonate-treated water, and 10 μl of TaqMan Gene Expression Master Mix (Thermo Fisher Scientific; 4369016). .. The qPCR program was as follows: UDG optimization at 50°C for 2 min, AmpliTaq Gold Activation at 95°C for 10 min, and 40 cycles of denaturation at 95°C for 15 sec followed by annealing/extension at 60°C for 1 min.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: .. TaqMan® Gene Expression Master Mix (Applied Biosystems) was mixed together with probes and primers for the selected targets (Sp4 Transcription Factor (SP4 ): Hs00162095_m1; Basic Helix-Loop-Helix Family Member E40 (BHLHE40 ): Hs00186419_m1; Calpain Small Subunit 1 (CAPNS1 ): Hs00998426_m1; Ras Homolog Family Member A (RHOA ): Hs01051295_m1; Regulator Of G Protein Signaling 16 (RGS16 ): Hs00892674_m1; Activator Of Transcription And Developmental Regulator AUTS2 (AUTS2 ): Hs01688766_m1; Kynurenine Aminotransferase 1 (KYAT1 ): Hs00187858). ..

    Derivative Assay:

    Article Title: Mulberry leaf reduces inflammation and insulin resistance in type 2 diabetic mice by TLRs and insulin Signalling pathway
    Article Snippet: The cDNA was amplified utilizing TaqMan Gene expression Master Mix (Applied Biosystems, USA) on CFX96. .. The amplification conditions started with Hold step at 50 °C for 2 min and 95 °C for 10 min, followed by 40 cycles with denaturing at 95 °C for 15 s, and extended at 60 °C for 60 s. Quantification of the mRNA was based on standard curves derived from cDNA standards for each primer pair.

    Article Title: Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming
    Article Snippet: For the TaqMan QRT-PCR panel, cDNA mixture of three parallel samples was used in case of each condition: control, 6, 24, and 72 h exosome treatment applied on MSC cells derived from four mice. .. Eighty nanogram cDNA and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) were used for the qPCR experiment.

    Concentration Assay:

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: Assay mixes were prepared by mixing 2.5 μl 2X Assay Loading Reagent (Fluidigm), 2.3 μl of primer pair mix (final concentration 1.15 μM) and 0.2 μl low EDTA TE buffer. .. Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA.

    Article Title: Structural analysis identifies an escape route from the adverse lipogenic effects of liver X receptor ligands
    Article Snippet: Concentration of total RNA was determined on a NanoDrop™ ND-100 Spectrophotometer (Saveen Werner, Lögstrup, Sweden). .. In the other studies, 400 nM Abca1 forward and reverse primers, 200 nM Abca1 probe, 200 nM Rplp0 forward and reverse primers, and 100 nM Rplp0 probe were mixed with 15 ng of cDNA template and TaqMan™ Gene Expression Master Mix (Applied Biosystems, Foster City, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Calcipotriol and betamethasone dipropionate synergistically enhances the balance between regulatory and proinflammatory T cells in a murine psoriasis model
    Article Snippet: Paragraph title: Real-time reverse transcription polymerase chain reaction (RT-PCR) ... RNA was obtained from ear skin samples with an RNeasy Fibrous Tissue Mini Kit (QIAGEN), and complementary DNA (cDNA) was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen Japan, Tokyo, Japan). cDNA, probes, and TaqMan Gene Expression Master Mix (Applied Biosystems, Tokyo, Japan) were then mixed in a 96-well PCR plate, and quantitative PCR was performed using an ABI StepOnePlusTM Real-Time PCR System (Applied Biosystems).

    Sequencing:

    Article Title: Microbiota analysis optimization for human bronchoalveolar lavage fluid
    Article Snippet: PAO1), and the number of 16S copies/ml was inferred using the URI Genomics & Sequencing Center online calculator ( http://cels.uri.edu/gsc/cndna.html ). .. 16S qPCR primers and conditions are described in Additional file . qPCR reactions were carried out in a volume of 11 μl using the TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol.

    Binding Assay:

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: .. Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. .. Thermal cycling conditions for qPCR were: thermal mix 50°C for 2 min, 70°C for 30 min, 25°C for 10 min, followed by hot start 50°C for 2 min, 95°C for 10 min, PCR (x30 cycles) 95°C for 15 sec, 60°C for 60 sec and melting curve analysis 60°C for 3 sec to 95°C.

    Article Title: High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats
    Article Snippet: .. The PCR reaction mixture contained cDNA samples, RNase-free water, TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, California, USA), and primers for following genes: Fatty acid synthase (Fasn ), acetyl-CoA carboxylase (Acaca ), stearoyl-CoA desaturase 1 (Scd1 ), MLX-interacting protein-like (Mlxipl) (known also as Carbohydrate-responsive element-binding protein (ChREBP )), and additionally in the liver on factors: Factor sterol regulatory element binding protein 1 (Srebf1 ), carnitine palmitoyltransferase 1a (Cpt1a ), and peroxisome proliferator-activated receptor alpha (Ppar-α ) with fluorescent marked starters. .. The reaction mixture was incubated for 2 min at 50 °C and for 10 min at 95 °C (initial step), as well as 40 cycles of 95 °C for 15 s (denaturation) and 60 °C for 1 min (annealing/extension) using CFX96 Touch™ Deep Well Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

    DNA Extraction:

    Article Title: Microbiota analysis optimization for human bronchoalveolar lavage fluid
    Article Snippet: Paragraph title: DNA isolation and quantification ... 16S qPCR primers and conditions are described in Additional file . qPCR reactions were carried out in a volume of 11 μl using the TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol.

    Isolation:

    Article Title: Metabolic Activation of Flavin Monooxygenase-mediated Trimethylamine-N-Oxide Formation in Experimental Kidney Disease
    Article Snippet: mRNA analysis RNA isolation and real-time quantitative polymerase chain reaction (RT-qPCR) were conducted as recommended by MIQE guidelines . .. Fmo3 , Fmo1 , Arnt , Ahr , Cyp1a2 , Cyp3a2 and Actb (β-Actin) genes were quantified by RT-qPCR with Applied Biosystems 7500 Fast Real-Time PCR System (Foster City, CA) using Taqman® Gene Expression Master Mix (Applied Biosystems, Foster City, CA) and Taqman® specific primers.

    Article Title: Calcipotriol and betamethasone dipropionate synergistically enhances the balance between regulatory and proinflammatory T cells in a murine psoriasis model
    Article Snippet: Real-time reverse transcription polymerase chain reaction (RT-PCR) Prior to the RNA isolation, the ear skin samples were homogenized with a TissueLyser (QIAGEN). .. RNA was obtained from ear skin samples with an RNeasy Fibrous Tissue Mini Kit (QIAGEN), and complementary DNA (cDNA) was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen Japan, Tokyo, Japan). cDNA, probes, and TaqMan Gene Expression Master Mix (Applied Biosystems, Tokyo, Japan) were then mixed in a 96-well PCR plate, and quantitative PCR was performed using an ABI StepOnePlusTM Real-Time PCR System (Applied Biosystems).

    Article Title: Mulberry leaf reduces inflammation and insulin resistance in type 2 diabetic mice by TLRs and insulin Signalling pathway
    Article Snippet: Total RNA was isolated from skeletal muscle using TRIZOL (Invitrogen, USA). .. The cDNA was amplified utilizing TaqMan Gene expression Master Mix (Applied Biosystems, USA) on CFX96.

    Article Title: Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming
    Article Snippet: Paragraph title: RNA Isolation, Reverse Transcription, and TaqMan Panel ... Eighty nanogram cDNA and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) were used for the qPCR experiment.

    Article Title: High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats
    Article Snippet: Gene Expression Total RNA from frozen liver and adipose tissue was isolated with Total RNA Mini Plus kit as well as Total RNA Mini kit, respectively (cat. no. 031-100 and 036-100, respectively, A & A Biotechnology, Gdynia, Poland), following the manufacturer’s protocols. .. The PCR reaction mixture contained cDNA samples, RNase-free water, TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, California, USA), and primers for following genes: Fatty acid synthase (Fasn ), acetyl-CoA carboxylase (Acaca ), stearoyl-CoA desaturase 1 (Scd1 ), MLX-interacting protein-like (Mlxipl) (known also as Carbohydrate-responsive element-binding protein (ChREBP )), and additionally in the liver on factors: Factor sterol regulatory element binding protein 1 (Srebf1 ), carnitine palmitoyltransferase 1a (Cpt1a ), and peroxisome proliferator-activated receptor alpha (Ppar-α ) with fluorescent marked starters.

    Size-exclusion Chromatography:

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. .. Thermal cycling conditions for qPCR were: thermal mix 50°C for 2 min, 70°C for 30 min, 25°C for 10 min, followed by hot start 50°C for 2 min, 95°C for 10 min, PCR (x30 cycles) 95°C for 15 sec, 60°C for 60 sec and melting curve analysis 60°C for 3 sec to 95°C.

    Article Title: Impact of peripheral immune status on central molecular responses to facial nerve axotomy
    Article Snippet: The 20 μl reaction volume contained 1 μl of cDNA, 1 μl of 20× TaqMan® FAM gene expression assay , 8 μl of 0.002% diethyl pyrocarbonate-treated water, and 10 μl of TaqMan Gene Expression Master Mix (Thermo Fisher Scientific; 4369016). .. The qPCR program was as follows: UDG optimization at 50°C for 2 min, AmpliTaq Gold Activation at 95°C for 10 min, and 40 cycles of denaturation at 95°C for 15 sec followed by annealing/extension at 60°C for 1 min.

    Purification:

    Article Title: Microbiota analysis optimization for human bronchoalveolar lavage fluid
    Article Snippet: DNA isolation and quantification Nucleic acids were isolated from 250 μl of sample using a PowerSoil DNA isolation kit (MO-BIO; Carlsbad, CA, USA) following the manufacturer’s instructions except for the elution step which was done in 60 μl purified water. .. 16S qPCR primers and conditions are described in Additional file . qPCR reactions were carried out in a volume of 11 μl using the TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. .. Thermal cycling conditions for qPCR were: thermal mix 50°C for 2 min, 70°C for 30 min, 25°C for 10 min, followed by hot start 50°C for 2 min, 95°C for 10 min, PCR (x30 cycles) 95°C for 15 sec, 60°C for 60 sec and melting curve analysis 60°C for 3 sec to 95°C.

    Article Title: Calcipotriol and betamethasone dipropionate synergistically enhances the balance between regulatory and proinflammatory T cells in a murine psoriasis model
    Article Snippet: .. RNA was obtained from ear skin samples with an RNeasy Fibrous Tissue Mini Kit (QIAGEN), and complementary DNA (cDNA) was synthesized using the SuperScript VILO cDNA Synthesis Kit (Invitrogen Japan, Tokyo, Japan). cDNA, probes, and TaqMan Gene Expression Master Mix (Applied Biosystems, Tokyo, Japan) were then mixed in a 96-well PCR plate, and quantitative PCR was performed using an ABI StepOnePlusTM Real-Time PCR System (Applied Biosystems). .. The following Taqman Gene Expression Assay probes were used in this study: Gapdh: Mm99999915_g1, Il17a : Mm00439619_m1, IL22 : Mm00444241_m1, Il23a : m00518984_m1 and Tnf : MM00443258_m1.

    Article Title: High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats
    Article Snippet: .. The PCR reaction mixture contained cDNA samples, RNase-free water, TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, California, USA), and primers for following genes: Fatty acid synthase (Fasn ), acetyl-CoA carboxylase (Acaca ), stearoyl-CoA desaturase 1 (Scd1 ), MLX-interacting protein-like (Mlxipl) (known also as Carbohydrate-responsive element-binding protein (ChREBP )), and additionally in the liver on factors: Factor sterol regulatory element binding protein 1 (Srebf1 ), carnitine palmitoyltransferase 1a (Cpt1a ), and peroxisome proliferator-activated receptor alpha (Ppar-α ) with fluorescent marked starters. .. The reaction mixture was incubated for 2 min at 50 °C and for 10 min at 95 °C (initial step), as well as 40 cycles of 95 °C for 15 s (denaturation) and 60 °C for 1 min (annealing/extension) using CFX96 Touch™ Deep Well Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

    Mouse Assay:

    Article Title: Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming
    Article Snippet: For the TaqMan QRT-PCR panel, cDNA mixture of three parallel samples was used in case of each condition: control, 6, 24, and 72 h exosome treatment applied on MSC cells derived from four mice. .. Eighty nanogram cDNA and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) were used for the qPCR experiment.

    Software:

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. .. Real-Time PCR Analysis software 3.1.3 (Fluidigm) was used to visualise results.

    Article Title: Structural analysis identifies an escape route from the adverse lipogenic effects of liver X receptor ligands
    Article Snippet: In the other studies, 400 nM Abca1 forward and reverse primers, 200 nM Abca1 probe, 200 nM Rplp0 forward and reverse primers, and 100 nM Rplp0 probe were mixed with 15 ng of cDNA template and TaqMan™ Gene Expression Master Mix (Applied Biosystems, Foster City, USA). .. Cycle threshold values (Ct) were retrieved from the instrument software.

    SYBR Green Assay:

    Article Title: Structural analysis identifies an escape route from the adverse lipogenic effects of liver X receptor ligands
    Article Snippet: In the study with BMS-852907, RT-qPCR reactions were carried out in separate triplicate wells for Abca1 and Rplp0, respectively, by using 20 ng of cDNA template, 385 nM of the above-mentioned primers, and iQ™ SYBR Green Supermix (Bio-Rad, Hercules, California, USA) on a Bio-Rad MyiQ instrument (Bio-Rad, Hercules, California, USA). .. In the other studies, 400 nM Abca1 forward and reverse primers, 200 nM Abca1 probe, 200 nM Rplp0 forward and reverse primers, and 100 nM Rplp0 probe were mixed with 15 ng of cDNA template and TaqMan™ Gene Expression Master Mix (Applied Biosystems, Foster City, USA).

    Quantitation Assay:

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA. .. Analysis settings were as follows: quality threshold was set to 0.65, baseline correction to linear (derivative) and quantitation cycle (Cq) threshold method to auto (global).

    Spectrophotometry:

    Article Title: Structural analysis identifies an escape route from the adverse lipogenic effects of liver X receptor ligands
    Article Snippet: Concentration of total RNA was determined on a NanoDrop™ ND-100 Spectrophotometer (Saveen Werner, Lögstrup, Sweden). .. In the other studies, 400 nM Abca1 forward and reverse primers, 200 nM Abca1 probe, 200 nM Rplp0 forward and reverse primers, and 100 nM Rplp0 probe were mixed with 15 ng of cDNA template and TaqMan™ Gene Expression Master Mix (Applied Biosystems, Foster City, USA).

    Article Title: Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming
    Article Snippet: RNA concentrations were measured by NanoDrop spectrophotometer (NanoDrop). cDNAs were reverse transcribed with TaqMan Reverse Transcription Reagents (Thermo Fisher Scientific) following the manufacturer's instruction. .. Eighty nanogram cDNA and TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) were used for the qPCR experiment.

    Article Title: High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats
    Article Snippet: The quantity of RNA and cDNA was measured with the Thermo Scientific μDrop Plate using spectrophotometer (Multiskan GO; Thermo Fisher Scientific Corporation, Vantaa, Finland). cDNA samples were stored at −20 °C until real-time PCR analysis. .. The PCR reaction mixture contained cDNA samples, RNase-free water, TaqMan Gene Expression Master Mix (Applied Biosystems, Foster City, California, USA), and primers for following genes: Fatty acid synthase (Fasn ), acetyl-CoA carboxylase (Acaca ), stearoyl-CoA desaturase 1 (Scd1 ), MLX-interacting protein-like (Mlxipl) (known also as Carbohydrate-responsive element-binding protein (ChREBP )), and additionally in the liver on factors: Factor sterol regulatory element binding protein 1 (Srebf1 ), carnitine palmitoyltransferase 1a (Cpt1a ), and peroxisome proliferator-activated receptor alpha (Ppar-α ) with fluorescent marked starters.

    Activation Assay:

    Article Title: Sitagliptin-Dependent Differences in the Intensity of Oxidative Stress in Rat Livers Subjected to Ischemia and Reperfusion
    Article Snippet: Real-Time PCR The mRNA expression of SOD1 was determined by real-time PCR with 7900HT Fast Real-Time PCR System and TaqMan Gene Expression Master Mix (Applied Biosystems, Carlsbad, CA, USA). .. All reactions were performed in triplicates in standardized thermal cycling conditions, including polymerase activation at 50°C for 2 min, denaturation at 94°C for 10 min, and 40 cycles of denaturation at 94°C for 15 s followed by annealing and synthesis at 60°C for 1 min.

    Article Title: Impact of peripheral immune status on central molecular responses to facial nerve axotomy
    Article Snippet: The 20 μl reaction volume contained 1 μl of cDNA, 1 μl of 20× TaqMan® FAM gene expression assay , 8 μl of 0.002% diethyl pyrocarbonate-treated water, and 10 μl of TaqMan Gene Expression Master Mix (Thermo Fisher Scientific; 4369016). .. The qPCR program was as follows: UDG optimization at 50°C for 2 min, AmpliTaq Gold Activation at 95°C for 10 min, and 40 cycles of denaturation at 95°C for 15 sec followed by annealing/extension at 60°C for 1 min.

    High Throughput Screening Assay:

    Article Title: Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
    Article Snippet: Paragraph title: High-throughput qPCR using the 96.96 Dynamic Array ... Sample mixes were prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA.

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    Thermo Fisher taqman gene expression master mix
    Taqman Gene Expression Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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