taqman gene expression assays  (Thermo Fisher)


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    Thermo Fisher taqman gene expression assays
    Validation of gene expression using <t>TaqMan®</t> real-time <t>PCR</t> on three control and three Parkinson's disease samples (). The following genes were selected: tyrosine hydroxylase (TH), dopamine transporter (DAT), Girk2 (KCNJ6), SNCA (PARK1),
    Taqman Gene Expression Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 6877 article reviews
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    Images

    1) Product Images from "Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology"

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology

    Journal:

    doi: 10.1093/brain/awn323

    Validation of gene expression using TaqMan® real-time PCR on three control and three Parkinson's disease samples (). The following genes were selected: tyrosine hydroxylase (TH), dopamine transporter (DAT), Girk2 (KCNJ6), SNCA (PARK1),
    Figure Legend Snippet: Validation of gene expression using TaqMan® real-time PCR on three control and three Parkinson's disease samples (). The following genes were selected: tyrosine hydroxylase (TH), dopamine transporter (DAT), Girk2 (KCNJ6), SNCA (PARK1),

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    TaqMan® real-time PCR assay validation
    Figure Legend Snippet: TaqMan® real-time PCR assay validation

    Techniques Used: Real-time Polymerase Chain Reaction

    2) Product Images from "A Crucial Role for Primary Cilia in Cortical Morphogenesis"

    Article Title: A Crucial Role for Primary Cilia in Cortical Morphogenesis

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2084-08.2008

    Targets of Shh signaling and Gli3 protein processing are disturbed in the forebrain of cbs mutants. A–D , In situ hybridization analysis of 12.5 wild-type and cbs/cbs embryos. For each coronal section, only one telencephalic half is shown, with dorsal to the top, lateral to the right. Scale bars: 300 μm. A , Gli3 . B , Emx1 . C , Emx2 . D , Ptch1 . E , Northern blots of whole RNA from forebrain of E12.5 wild-type and cbs / cbs embryos. Full-length Gli3 (top) and α-tubulin cDNAs (bottom) were used as probes. Ribosomal RNA markers are to the left. F , Western blots of protein from forebrain of E12.5 cbs and Xt J wild-type (+/+) and homozygous mutant (−/−) embryos. An anti-N-terminal-Gli3 antibody (top) and an anti-β-actin antibody (bottom) were used. Specific bands corresponding to the 190 kDa full-length Gli3 isoform (arrows) and the 80 kDa proteolytically processed Gli3 isoform (arrowhea ds) are indicated. The specificity of the antibody was shown by examining homozygous Gli3 deletion mutants ( Xt J ), in which neither full-length nor processed Gli3 isoforms are detectable. Protein markers (kDa) are to the left. G , Quantitation of Gli3 Western blots seen in ( F ), first indicating the amount of the 90 kDa (short form) Gli3 isoform, setting levels in +/+ to 1.0. A quantitative comparison of the 190 kDa (long form) isoform shows 19.4% levels in +/+ embryos, compared with the short form. cbs/cbs mutants show a 5.6-fold increase in the amount of the long form, compared with +/+ embryos, to levels greater than that of the short form in cbs/cbs embryos. The combined amount of short and long isoforms is also indicated (total). H , Quantitative real time RT-PCR was performed upon mRNA extracted from E12.5 telencephalon. Reverse-transcribed cDNA was analyzed using TaqMan probes recognizing Ptch1 and Gli1 . cDNA was normalized using probes for GAPDH. I , Luciferase assays using a Gli-responsive luciferase plasmid transiently transfected into fibroblasts prepared from wild-type and cbs/cbs embryos and allowed to reach confluency. Sonic hedgehog (Shh, 1 μg/ml) was added to the cultures for 12 h before lysis and analysis of luciferase levels. Relative luminosity levels relative to Shh-untreated wild-type cells are indicated. G–I , Mean values ± SEM ( n = 4–8). *** p
    Figure Legend Snippet: Targets of Shh signaling and Gli3 protein processing are disturbed in the forebrain of cbs mutants. A–D , In situ hybridization analysis of 12.5 wild-type and cbs/cbs embryos. For each coronal section, only one telencephalic half is shown, with dorsal to the top, lateral to the right. Scale bars: 300 μm. A , Gli3 . B , Emx1 . C , Emx2 . D , Ptch1 . E , Northern blots of whole RNA from forebrain of E12.5 wild-type and cbs / cbs embryos. Full-length Gli3 (top) and α-tubulin cDNAs (bottom) were used as probes. Ribosomal RNA markers are to the left. F , Western blots of protein from forebrain of E12.5 cbs and Xt J wild-type (+/+) and homozygous mutant (−/−) embryos. An anti-N-terminal-Gli3 antibody (top) and an anti-β-actin antibody (bottom) were used. Specific bands corresponding to the 190 kDa full-length Gli3 isoform (arrows) and the 80 kDa proteolytically processed Gli3 isoform (arrowhea ds) are indicated. The specificity of the antibody was shown by examining homozygous Gli3 deletion mutants ( Xt J ), in which neither full-length nor processed Gli3 isoforms are detectable. Protein markers (kDa) are to the left. G , Quantitation of Gli3 Western blots seen in ( F ), first indicating the amount of the 90 kDa (short form) Gli3 isoform, setting levels in +/+ to 1.0. A quantitative comparison of the 190 kDa (long form) isoform shows 19.4% levels in +/+ embryos, compared with the short form. cbs/cbs mutants show a 5.6-fold increase in the amount of the long form, compared with +/+ embryos, to levels greater than that of the short form in cbs/cbs embryos. The combined amount of short and long isoforms is also indicated (total). H , Quantitative real time RT-PCR was performed upon mRNA extracted from E12.5 telencephalon. Reverse-transcribed cDNA was analyzed using TaqMan probes recognizing Ptch1 and Gli1 . cDNA was normalized using probes for GAPDH. I , Luciferase assays using a Gli-responsive luciferase plasmid transiently transfected into fibroblasts prepared from wild-type and cbs/cbs embryos and allowed to reach confluency. Sonic hedgehog (Shh, 1 μg/ml) was added to the cultures for 12 h before lysis and analysis of luciferase levels. Relative luminosity levels relative to Shh-untreated wild-type cells are indicated. G–I , Mean values ± SEM ( n = 4–8). *** p

    Techniques Used: In Situ Hybridization, Northern Blot, Western Blot, Mutagenesis, Quantitation Assay, Quantitative RT-PCR, Luciferase, Plasmid Preparation, Transfection, Lysis

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    Synthesized:

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    Quantitative RT-PCR:

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    Real-time Polymerase Chain Reaction:

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    Quantitation Assay:

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    Expressing:

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    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
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    Modification:

    Article Title: Repository corticotrophin injection exerts direct acute effects on human B cell gene expression distinct from the actions of glucocorticoids
    Article Snippet: Total RNA was isolated from frozen CD19+ cell pellets using the RNeasy Mini Kit (Qiagen) and following a modified version of the manufacturer's protocol. .. Each DNase‐treated RNA sample was subjected to reverse transcription using a high‐capacity RNA to cDNA Kit (Applied Biosystems, Foster City, CA, USA). cDNA was amplified using TaqMan Gene Expression Master Mix and TaqMan Gene Expression Assays in the QuantStudio 12K Flex Real‐Time PCR System (Applied Biosystems) with the following parameters: 50°C for 2 min, 95°C for 10 min and 40 cycles of 95°C for 15 s and 60°C for 1 min. TaqMan gene expression assays (Life Technologies, Carlsbad, CA, USA) are shown in Table .

    Transformation Assay:

    Article Title: Validation Study of an Operational Tolerance Signature in Korean Kidney Transplant Recipients
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    Cell Culture:

    Article Title: Tumor microenvironment profoundly modifies functional status of macrophages: Peritoneal and tumor-associated macrophages are two very different subpopulations
    Article Snippet: .. 107 cells from individual samples of N-PEMs, T-PEMs and TAMs were cultured in complete medium (RPMI with 10% FBS), constitutively and with 10 μg/mL LPS for 2 h. Total RNA was isolated and cDNA synthesis was performed using Invitrogen reagents. qRT-PCR was done using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA) for IL-12p35, IL-12p40, IL-23p19 and GAPDH 20X on a 7500 Fast Real-time PCR system (Applied Biosystems, Carlsbad, CA). ..

    Generated:

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: Expression of 14 genes (listed below) was measured in three normal control and three Parkinson's disease samples ( ) by real-time PCR using TaqMan® Gene Expression Assays and the 7900HT Real-Time PCR System (Applied Biosystems, Foster City, CA). .. A total of 250–600 DA neurons were captured from each sample and total RNA isolated using the mir VANA™ miRNA Isolation Kit (Ambion). cDNAs were generated in a 25 µl reverse transcription reaction with 60 ng of total RNA from each sample using the High Capacity cDNA Archive Kit and protocol (Applied Biosystems, PN 4322169).

    Polymerase Chain Reaction:

    Article Title: Fetal exposure to maternal inflammation interrupts murine intestinal development and increases susceptibility to neonatal intestinal injury
    Article Snippet: .. qRT-PCR Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed using Taqman Fast Universal PCR Master Mix (2×) (Life Technologies) and Taqman Gene Expression Assays for Muc2 , Defa1 , cleaved caspase 3 and Ki67 (Life Technologies). qRT-PCR reactions were run in a C1000 Thermal Cycler (Eppendorf) and using the CFX96 Real-Time PCR Detection System (Bio-Rad). .. Fold change in gene expression was determined by normalizing gene expression to β-actin in each sample.

    Article Title: Fine specificity of natural killer T cells against GD3 ganglioside and identification of GM3 as an inhibitory natural killer T-cell ligand
    Article Snippet: .. Real-time PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) with TaqMan® Gene Expression Assays as a primer and TaqMan® Universal PCR Master Mix as a polymerase (Applied Biosystems). .. The gene expression level was analysed by S equence D etection software version 1.2.3.

    Article Title: Validation Study of an Operational Tolerance Signature in Korean Kidney Transplant Recipients
    Article Snippet: TaqMan gene expression assays (StepOnePlus™; Applied Biosystems, Foster City, CA, USA) were performed under the standard TaqMan protocol (10 min at 95°C, 40 cycles of 15 s at 95°C, and 60 s at 60°C) in a 96-well microplate. .. We used primers and probes purchased commercially from Integrated DNA Technologies (Coralville, IA, USA) for PCR assays.

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: Expression of 14 genes (listed below) was measured in three normal control and three Parkinson's disease samples ( ) by real-time PCR using TaqMan® Gene Expression Assays and the 7900HT Real-Time PCR System (Applied Biosystems, Foster City, CA). .. The resulting cDNA was subjected to a 10-cycle PCR amplification followed by real-time PCR reaction using the manufacturer's TaqMan® PreAmp Master Mix Kit Protocol (Applied Biosystems, PN 4366127).

    Binding Assay:

    Article Title: Inhibition of Nox4‐dependent ROS signaling attenuates prostate fibroblast activation and abrogates stromal‐mediated protumorigenic interactions
    Article Snippet: RNA isolation, cDNA synthesis and qPCR using Taqman® gene expression assays were performed as described., TaqMan® gene expression assays (Applied Biosystems, Vienna, Austria) are listed in Supporting Information, Table 1. .. Data are presented as fold change in gene expression using the mathematical model ratio 2−ΔΔCT or as mean 2−ΔCT defined as 2−[Ct housekeeping gene − Ct target gene] thus providing an indication of the level of target gene expression relative to the moderately expressed housekeeping gene TATA binding protein (TBP).

    Article Title: Inhibition of Nox4‐dependent ROS signaling attenuates prostate fibroblast activation and abrogates stromal‐mediated protumorigenic interactions
    Article Snippet: RNA isolation, cDNA synthesis and quantitative real‐time PCR (qPCR) RNA isolation, cDNA synthesis and qPCR using Taqman® gene expression assays were performed as described., TaqMan® gene expression assays (Applied Biosystems, Vienna, Austria) are listed in Supporting Information, Table 1. .. Data are presented as fold change in gene expression using the mathematical model ratio 2−ΔΔCT or as mean 2−ΔCT defined as 2−[Ct housekeeping gene − Ct target gene] thus providing an indication of the level of target gene expression relative to the moderately expressed housekeeping gene TATA binding protein (TBP).

    RNA Sequencing Assay:

    Article Title: Transcriptome-wide analysis associates ID2 expression with combined pre- and post-capillary pulmonary hypertension
    Article Snippet: Expression was measured in each of the differentially expressed genes identified in RNA-seq analysis using quantitative real-time PCR (qRT-PCR). .. Gene expression was then measured using TaqMan® gene expression assays (Thermo Fisher Scientific, Waltham, MA, USA).

    RNA HS Assay:

    Article Title: Repository corticotrophin injection exerts direct acute effects on human B cell gene expression distinct from the actions of glucocorticoids
    Article Snippet: The concentration of the RNA in the resulting supernatant was determined using the Qubit RNA HS Assay Kit (Thermo Scientific, Waltham, MA, USA) in conjunction with a Qubit Fluorometer. .. Each DNase‐treated RNA sample was subjected to reverse transcription using a high‐capacity RNA to cDNA Kit (Applied Biosystems, Foster City, CA, USA). cDNA was amplified using TaqMan Gene Expression Master Mix and TaqMan Gene Expression Assays in the QuantStudio 12K Flex Real‐Time PCR System (Applied Biosystems) with the following parameters: 50°C for 2 min, 95°C for 10 min and 40 cycles of 95°C for 15 s and 60°C for 1 min. TaqMan gene expression assays (Life Technologies, Carlsbad, CA, USA) are shown in Table .

    Isolation:

    Article Title: Inhibition of Nox4‐dependent ROS signaling attenuates prostate fibroblast activation and abrogates stromal‐mediated protumorigenic interactions
    Article Snippet: .. RNA isolation, cDNA synthesis and qPCR using Taqman® gene expression assays were performed as described., TaqMan® gene expression assays (Applied Biosystems, Vienna, Austria) are listed in Supporting Information, Table 1. .. Data are presented as fold change in gene expression using the mathematical model ratio 2−ΔΔCT or as mean 2−ΔCT defined as 2−[Ct housekeeping gene − Ct target gene] thus providing an indication of the level of target gene expression relative to the moderately expressed housekeeping gene TATA binding protein (TBP).

    Article Title: Transcriptome-wide analysis associates ID2 expression with combined pre- and post-capillary pulmonary hypertension
    Article Snippet: RNA was isolated and reverse transcribed to complementary DNA. .. Gene expression was then measured using TaqMan® gene expression assays (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Repository corticotrophin injection exerts direct acute effects on human B cell gene expression distinct from the actions of glucocorticoids
    Article Snippet: Total RNA was isolated from frozen CD19+ cell pellets using the RNeasy Mini Kit (Qiagen) and following a modified version of the manufacturer's protocol. .. Each DNase‐treated RNA sample was subjected to reverse transcription using a high‐capacity RNA to cDNA Kit (Applied Biosystems, Foster City, CA, USA). cDNA was amplified using TaqMan Gene Expression Master Mix and TaqMan Gene Expression Assays in the QuantStudio 12K Flex Real‐Time PCR System (Applied Biosystems) with the following parameters: 50°C for 2 min, 95°C for 10 min and 40 cycles of 95°C for 15 s and 60°C for 1 min. TaqMan gene expression assays (Life Technologies, Carlsbad, CA, USA) are shown in Table .

    Article Title: Hyperbaric Normoxia Improved Glucose Metabolism and Decreased Inflammation in Obese Diabetic Rat
    Article Snippet: Total RNA was isolated with a TRIzol reagent (15596-026; Invitrogen, Tokyo, Japan). .. The mRNA expression levels of IL-6 (Rn01410330_m1), TNFα (Rn01525859_g1), IL-1β (Rn00580432_m1), and IL-10 (Rn00563409_m1) were quantified by qPCR with TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA).

    Article Title: Influence of the Tissue Microenvironment on Toll-Like Receptor Expression by CD11c+ Antigen-Presenting Cells Isolated from Mucosal Tissues ▿
    Article Snippet: Total RNA was isolated using an RNeasy minikit (Qiagen), and cDNA was synthesized using a RETROscript kit (Ambion) according to the manufacturer's instructions. .. Real-time PCR gene expression analysis was conducted using an ABI Prism 7900HT apparatus (Applied Biosystems) and TaqMan gene expression assays for TLR2, TRL4, TLR5, TLR9, and ribosomal protein L32 (Applied Biosystems).

    Article Title: Inhibition of Nox4‐dependent ROS signaling attenuates prostate fibroblast activation and abrogates stromal‐mediated protumorigenic interactions
    Article Snippet: .. RNA isolation, cDNA synthesis and quantitative real‐time PCR (qPCR) RNA isolation, cDNA synthesis and qPCR using Taqman® gene expression assays were performed as described., TaqMan® gene expression assays (Applied Biosystems, Vienna, Austria) are listed in Supporting Information, Table 1. .. Data are presented as fold change in gene expression using the mathematical model ratio 2−ΔΔCT or as mean 2−ΔCT defined as 2−[Ct housekeeping gene − Ct target gene] thus providing an indication of the level of target gene expression relative to the moderately expressed housekeeping gene TATA binding protein (TBP).

    Article Title: Tumor microenvironment profoundly modifies functional status of macrophages: Peritoneal and tumor-associated macrophages are two very different subpopulations
    Article Snippet: .. 107 cells from individual samples of N-PEMs, T-PEMs and TAMs were cultured in complete medium (RPMI with 10% FBS), constitutively and with 10 μg/mL LPS for 2 h. Total RNA was isolated and cDNA synthesis was performed using Invitrogen reagents. qRT-PCR was done using TaqMan Gene Expression Assays (Applied Biosystems, Carlsbad, CA) for IL-12p35, IL-12p40, IL-23p19 and GAPDH 20X on a 7500 Fast Real-time PCR system (Applied Biosystems, Carlsbad, CA). ..

    Mouse Assay:

    Article Title: The Role of the Voltage-Gated Potassium Channel Proteins Kv8.2 and Kv2.1 in Vision and Retinal Disease: Insights from the Study of Mouse Gene Knock-Out Mutations
    Article Snippet: .. TaqMan Gene Expression Assays (Thermo Fisher Scientific, mouse genes) were used to assess the relative transcript levels of Kcnv2 (Mm00807577_m1) in WT and Kv8.2 KO mice, normalized to Gapdh (Mm99999915_g1). .. The results confirmed the complete absence of Kcnv2 transcripts in the retinae of Kv8.2 KO mice.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice
    Article Snippet: .. Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was then performed with a 7900HT Real-Time PCR System using TaqMan Gene Expression Master Mix (Grand Island, NY; 4369016) and TaqMan Gene-Expression Assays (Applied Biosystems, Foster City, CA) for the following genes: chemokine (C-C motif) ligand 2 ( CCL2 ; Mm00441242_m1), CCL3 (Mm00441259_g1), chemokine (C-X-C motif) ligand 1 ( CXCL1 ; Mm04207460_m1), intercellular adhesion molecule 1 ( ICAM1 ; Mm00516023_m1), interleukin (IL)-1β ( IL-1β ; Mm00434228_m1), IL-10 (Mm01288386_m1), tumor necrosis factor-α ( TNF-α ; Mm00443258_m1), and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ; Mm99999915_g1). ..

    Article Title: Fetal exposure to maternal inflammation interrupts murine intestinal development and increases susceptibility to neonatal intestinal injury
    Article Snippet: .. qRT-PCR Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed using Taqman Fast Universal PCR Master Mix (2×) (Life Technologies) and Taqman Gene Expression Assays for Muc2 , Defa1 , cleaved caspase 3 and Ki67 (Life Technologies). qRT-PCR reactions were run in a C1000 Thermal Cycler (Eppendorf) and using the CFX96 Real-Time PCR Detection System (Bio-Rad). .. Fold change in gene expression was determined by normalizing gene expression to β-actin in each sample.

    IA:

    Article Title: Validation Study of an Operational Tolerance Signature in Korean Kidney Transplant Recipients
    Article Snippet: TaqMan gene expression assays (StepOnePlus™; Applied Biosystems, Foster City, CA, USA) were performed under the standard TaqMan protocol (10 min at 95°C, 40 cycles of 15 s at 95°C, and 60 s at 60°C) in a 96-well microplate. .. We used primers and probes purchased commercially from Integrated DNA Technologies (Coralville, IA, USA) for PCR assays.

    Software:

    Article Title: Fine specificity of natural killer T cells against GD3 ganglioside and identification of GM3 as an inhibitory natural killer T-cell ligand
    Article Snippet: Real-time PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) with TaqMan® Gene Expression Assays as a primer and TaqMan® Universal PCR Master Mix as a polymerase (Applied Biosystems). .. The gene expression level was analysed by S equence D etection software version 1.2.3.

    Histone Deacetylase Assay:

    Article Title: Repository corticotrophin injection exerts direct acute effects on human B cell gene expression distinct from the actions of glucocorticoids
    Article Snippet: Each DNase‐treated RNA sample was subjected to reverse transcription using a high‐capacity RNA to cDNA Kit (Applied Biosystems, Foster City, CA, USA). cDNA was amplified using TaqMan Gene Expression Master Mix and TaqMan Gene Expression Assays in the QuantStudio 12K Flex Real‐Time PCR System (Applied Biosystems) with the following parameters: 50°C for 2 min, 95°C for 10 min and 40 cycles of 95°C for 15 s and 60°C for 1 min. TaqMan gene expression assays (Life Technologies, Carlsbad, CA, USA) are shown in Table . .. The comparative Ct method was utilized by the QuantStudio software to calculate the fold difference in expression of a target gene relative to that of the endogenous control genes of RAR‐related orphan receptor A (RORA) and histone deacetylase 5 (HDAC5) for each sample (RORA and HDAC5 mRNA levels were previously determined experimentally to be invariant between resting and activated B cells; data not shown).

    Spectrophotometry:

    Article Title: Validation Study of an Operational Tolerance Signature in Korean Kidney Transplant Recipients
    Article Snippet: Total RNA was extracted using a PAXgene Blood RNA Kit (PreAnalytiX) according to the manufacturer's instructions and was measured for quantity and purity using a NanoDrop® ND-2000 UV spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA synthesis was performed with 500 ng of total RNA using an M-MLV Reverse Transcriptase system (200 U/µl; Mbiotech, Inc., Seoul, Korea), and cDNA was stored at −80°C until use. .. TaqMan gene expression assays (StepOnePlus™; Applied Biosystems, Foster City, CA, USA) were performed under the standard TaqMan protocol (10 min at 95°C, 40 cycles of 15 s at 95°C, and 60 s at 60°C) in a 96-well microplate.

    Article Title: Hyperbaric Normoxia Improved Glucose Metabolism and Decreased Inflammation in Obese Diabetic Rat
    Article Snippet: The total RNA concentration was determined with a spectrophotometer (NanoDrop; Thermo Fisher Scientific, Waltham, MA, USA) and calibrated to be equal for all samples. .. The mRNA expression levels of IL-6 (Rn01410330_m1), TNFα (Rn01525859_g1), IL-1β (Rn00580432_m1), and IL-10 (Rn00563409_m1) were quantified by qPCR with TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA).

    Concentration Assay:

    Article Title: Repository corticotrophin injection exerts direct acute effects on human B cell gene expression distinct from the actions of glucocorticoids
    Article Snippet: The concentration of the RNA in the resulting supernatant was determined using the Qubit RNA HS Assay Kit (Thermo Scientific, Waltham, MA, USA) in conjunction with a Qubit Fluorometer. .. Each DNase‐treated RNA sample was subjected to reverse transcription using a high‐capacity RNA to cDNA Kit (Applied Biosystems, Foster City, CA, USA). cDNA was amplified using TaqMan Gene Expression Master Mix and TaqMan Gene Expression Assays in the QuantStudio 12K Flex Real‐Time PCR System (Applied Biosystems) with the following parameters: 50°C for 2 min, 95°C for 10 min and 40 cycles of 95°C for 15 s and 60°C for 1 min. TaqMan gene expression assays (Life Technologies, Carlsbad, CA, USA) are shown in Table .

    Article Title: Hyperbaric Normoxia Improved Glucose Metabolism and Decreased Inflammation in Obese Diabetic Rat
    Article Snippet: The total RNA concentration was determined with a spectrophotometer (NanoDrop; Thermo Fisher Scientific, Waltham, MA, USA) and calibrated to be equal for all samples. .. The mRNA expression levels of IL-6 (Rn01410330_m1), TNFα (Rn01525859_g1), IL-1β (Rn00580432_m1), and IL-10 (Rn00563409_m1) were quantified by qPCR with TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA).

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    Thermo Fisher taqmangene expression assay
    Taqmangene Expression Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    taqmangene expression assay - by Bioz Stars, 2020-01
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    The Applied Biosystems TaqMan Array Human IL 2 Gene Expression In Activated And Quiescent T Cells 96 well Plate contains 28 assays to IL 2 gene expression in activated and
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