taqman detection system  (Thermo Fisher)


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    Thermo Fisher taqman detection system
    Taqman Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman detection system/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    taqman detection system - by Bioz Stars, 2020-02
    99/100 stars

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    Clone Assay:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Amplification:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    DNA Synthesis:

    Article Title: Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex
    Article Snippet: Complementary DNA synthesis from 1 μ g RNA was performed using Superscript-first-strand synthesis systems for RT–PCR (Invitrogen, Carlsbad, CA, USA). .. The transcript levels of GREB1, RERG, GPER, BCAR1, BCAR3, CDK6, ERBB2 and ABCG2 were investigated by real-time PCR using Taqman detection system (Applied Biosystems, Carlsbad, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157
    Article Snippet: .. The TaqMan detection system (Applied Biosystems, Foster City, Calif.) is a new qualitative and quantitative system that uses a fluorogenic hybridization probe to detect the target genes; and it has previously been demonstrated to be a rapid, high-throughput, semiautomatic PCR scheme for the identification of E. coli ( , ), Salmonella , and Listeria spp. ( ). .. The objective of this study was to assess the utility of the TaqMan PCR system for the detection and identification of the stx 2 gene (which is responsible for the biosynthesis of Shiga-like toxin 2) and the rfbE. coli O157 gene (which is responsible for the biosynthesis of the O antigen) ( ).

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Article Title: The combination astemizole–gefitinib as a potential therapy for human lung cancer
    Article Snippet: .. RT-PCR was performed with 1 μL of cDNA using the TaqMan™ detection system (Thermo Fisher Scientific) and the Universal PCR Master Mix reagents kit (Thermo Fisher Scientific). .. Probes previously developed from TaqMan were used to study Eag1 (ID: Hs00924320_m1) and Gusb (ID: Hs00939627_m1, as a constitutive gene) expression.

    TA Cloning:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Construct:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: Validation of the assay with international standards For validation of the assay, the international panel cited above was used in qPCR reactions to construct standard curves of HBV DNA or HCV RNA in the following concentrations: HBV 7 or HCV 7 (5,000,000 IU/mL), HCV 6 or HBV 6 (500,000 IU/mL), HBV 5 or HCV 5 (50,000 IU/mL), HBV 4 or HCV 4 (5000 IU/mL), HBV 3 or HCV 3 (500 IU/mL), and HBV 2 or HCV 2 (50 IU/mL). .. The reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Thermo Fisher Scientific, Waltham, MA, USA) with 12.5 μL of TaqMan Universal Master Buffer, predetermined concentrations of the primer–probe sets cited above, and 50 - 100ng of DNA or cDNA, for a total final volume of 25 μL per reaction.

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: Quantification of viral loads To quantify the viral loads, standard curves were constructed from a dilution series of cloned plasmids containing inserts in the conserved region of each virus in initial concentrations of 5.1 × 109 IU/mL and 4.9 × 109 IU/mL for HBV and HCV, respectively, with a total of eight points in each curve. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157
    Article Snippet: In recent years, some molecular methods were developed to detect and identify this food-borne pathogen, such as PCR and enzyme-linked immunosorbent assay. .. The TaqMan detection system (Applied Biosystems, Foster City, Calif.) is a new qualitative and quantitative system that uses a fluorogenic hybridization probe to detect the target genes; and it has previously been demonstrated to be a rapid, high-throughput, semiautomatic PCR scheme for the identification of E. coli ( , ), Salmonella , and Listeria spp. ( ).

    Expressing:

    Article Title: The combination astemizole–gefitinib as a potential therapy for human lung cancer
    Article Snippet: RT-PCR was performed with 1 μL of cDNA using the TaqMan™ detection system (Thermo Fisher Scientific) and the Universal PCR Master Mix reagents kit (Thermo Fisher Scientific). .. Probes previously developed from TaqMan were used to study Eag1 (ID: Hs00924320_m1) and Gusb (ID: Hs00939627_m1, as a constitutive gene) expression.

    Article Title: Oxytocin, vasopressin and estrogen receptor gene expression in relation to social recognition in female mice
    Article Snippet: For each gene, qRT-PCR was carried out in all brain regions, using Applied Biosystems' gene expression assays (Foster City, CA): ERα (Assay ID Mm00433149_m1), ERβ (Assay ID Mm00599819_m1), PR (Assay ID Mm00435628_m1), OT (Assay ID Mm01329577_g1), OTR (Assay ID Mm01182684_m1), AVP (Assay ID Mm00437761_g1), AVPR1a (Assay ID Mm00444092_m1), and AVPR1b (Assay ID Mm01700416_m1). .. Q-PCR reactions were carried out using the Taqman detection system (ABI Prism® 7000 Sequence Detection System, Applied Biosystems, Foster City, CA) and the following conditions: 2 min at 50 °C, 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 1 min at 60 °C.

    Article Title: Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †
    Article Snippet: .. Validation of Differentially Expressed MiRNAs in Offspring In order to validate the expressions of differential miRNAs, quantitative real-time PCR (qRT-PCR) were utilized to detect the relative expression of differentially-expressed miRNAs, with samples enlarged in each group (n = 6 to 8 per group) using the TaqMan detection system (Life Technologies, Foster City, CA, USA). .. Total RNA was reversely transcribed using the TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, Life Technologies, Foster City, CA, USA).

    Article Title: Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex
    Article Snippet: The transcript levels of GREB1, RERG, GPER, BCAR1, BCAR3, CDK6, ERBB2 and ABCG2 were investigated by real-time PCR using Taqman detection system (Applied Biosystems, Carlsbad, CA, USA). .. The expression of the housekeeping gene glyceralaldehyde 3-phosphate dehydrogenase (GAPDH ) served as an internal control.

    Article Title: Aldehyde dehydrogenase-1a1 induces oncogene suppressor genes in B cell populations
    Article Snippet: Total RNA was prepared using the RNeasy kit (Qiagen, Valencia, CA). qRT-PCR was performed with predesigned assays (Applied Biosystems, Foster City, CA) using on a 7900HT Fast Real-Time PCR System, TaqMan detection system, and validated primers (Applied Biosystems, Foster City, CA) in triplicate as described [ ]. .. The mRNA expression was calculated based on Tata-box binding protein (TBP) expression for normalization using the comparative Ct method.

    Real-time Polymerase Chain Reaction:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: .. The reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Thermo Fisher Scientific, Waltham, MA, USA) with 12.5 μL of TaqMan Universal Master Buffer, predetermined concentrations of the primer–probe sets cited above, and 50 - 100ng of DNA or cDNA, for a total final volume of 25 μL per reaction. ..

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: The combination astemizole–gefitinib as a potential therapy for human lung cancer
    Article Snippet: Paragraph title: Real-time polymerase chain reaction (RT-PCR) ... RT-PCR was performed with 1 μL of cDNA using the TaqMan™ detection system (Thermo Fisher Scientific) and the Universal PCR Master Mix reagents kit (Thermo Fisher Scientific).

    Article Title: Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †
    Article Snippet: .. Validation of Differentially Expressed MiRNAs in Offspring In order to validate the expressions of differential miRNAs, quantitative real-time PCR (qRT-PCR) were utilized to detect the relative expression of differentially-expressed miRNAs, with samples enlarged in each group (n = 6 to 8 per group) using the TaqMan detection system (Life Technologies, Foster City, CA, USA). .. Total RNA was reversely transcribed using the TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, Life Technologies, Foster City, CA, USA).

    Article Title: Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex
    Article Snippet: .. The transcript levels of GREB1, RERG, GPER, BCAR1, BCAR3, CDK6, ERBB2 and ABCG2 were investigated by real-time PCR using Taqman detection system (Applied Biosystems, Carlsbad, CA, USA). .. The expression of the housekeeping gene glyceralaldehyde 3-phosphate dehydrogenase (GAPDH ) served as an internal control.

    Article Title: Aldehyde dehydrogenase-1a1 induces oncogene suppressor genes in B cell populations
    Article Snippet: .. Total RNA was prepared using the RNeasy kit (Qiagen, Valencia, CA). qRT-PCR was performed with predesigned assays (Applied Biosystems, Foster City, CA) using on a 7900HT Fast Real-Time PCR System, TaqMan detection system, and validated primers (Applied Biosystems, Foster City, CA) in triplicate as described [ ]. .. The mRNA expression was calculated based on Tata-box binding protein (TBP) expression for normalization using the comparative Ct method.

    Hybridization:

    Article Title: Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157
    Article Snippet: .. The TaqMan detection system (Applied Biosystems, Foster City, Calif.) is a new qualitative and quantitative system that uses a fluorogenic hybridization probe to detect the target genes; and it has previously been demonstrated to be a rapid, high-throughput, semiautomatic PCR scheme for the identification of E. coli ( , ), Salmonella , and Listeria spp. ( ). .. The objective of this study was to assess the utility of the TaqMan PCR system for the detection and identification of the stx 2 gene (which is responsible for the biosynthesis of Shiga-like toxin 2) and the rfbE. coli O157 gene (which is responsible for the biosynthesis of the O antigen) ( ).

    TaqMan microRNA Assay:

    Article Title: Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †
    Article Snippet: Validation of Differentially Expressed MiRNAs in Offspring In order to validate the expressions of differential miRNAs, quantitative real-time PCR (qRT-PCR) were utilized to detect the relative expression of differentially-expressed miRNAs, with samples enlarged in each group (n = 6 to 8 per group) using the TaqMan detection system (Life Technologies, Foster City, CA, USA). .. All of the miRNA-specific reverse-transcription primers were provided with the TaqMan MicroRNA Assay and purchased from Life Technologies Corporation (Applied Biosystems, Life Technologies, Foster City, CA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The combination astemizole–gefitinib as a potential therapy for human lung cancer
    Article Snippet: .. RT-PCR was performed with 1 μL of cDNA using the TaqMan™ detection system (Thermo Fisher Scientific) and the Universal PCR Master Mix reagents kit (Thermo Fisher Scientific). .. Probes previously developed from TaqMan were used to study Eag1 (ID: Hs00924320_m1) and Gusb (ID: Hs00939627_m1, as a constitutive gene) expression.

    Article Title: Oxytocin, vasopressin and estrogen receptor gene expression in relation to social recognition in female mice
    Article Snippet: Paragraph title: 2.5. Quantitative real time reverse transcription polymerase chain reaction ... Q-PCR reactions were carried out using the Taqman detection system (ABI Prism® 7000 Sequence Detection System, Applied Biosystems, Foster City, CA) and the following conditions: 2 min at 50 °C, 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 1 min at 60 °C.

    Article Title: Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex
    Article Snippet: Complementary DNA synthesis from 1 μ g RNA was performed using Superscript-first-strand synthesis systems for RT–PCR (Invitrogen, Carlsbad, CA, USA). .. The transcript levels of GREB1, RERG, GPER, BCAR1, BCAR3, CDK6, ERBB2 and ABCG2 were investigated by real-time PCR using Taqman detection system (Applied Biosystems, Carlsbad, CA, USA).

    Electroporation Bacterial Transformation:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Binding Assay:

    Article Title: Aldehyde dehydrogenase-1a1 induces oncogene suppressor genes in B cell populations
    Article Snippet: Total RNA was prepared using the RNeasy kit (Qiagen, Valencia, CA). qRT-PCR was performed with predesigned assays (Applied Biosystems, Foster City, CA) using on a 7900HT Fast Real-Time PCR System, TaqMan detection system, and validated primers (Applied Biosystems, Foster City, CA) in triplicate as described [ ]. .. The mRNA expression was calculated based on Tata-box binding protein (TBP) expression for normalization using the comparative Ct method.

    Isolation:

    Article Title: Aldehyde dehydrogenase-1a1 induces oncogene suppressor genes in B cell populations
    Article Snippet: Paragraph title: RNA isolation and quantitative real time PCR (qRT-PCR) ... Total RNA was prepared using the RNeasy kit (Qiagen, Valencia, CA). qRT-PCR was performed with predesigned assays (Applied Biosystems, Foster City, CA) using on a 7900HT Fast Real-Time PCR System, TaqMan detection system, and validated primers (Applied Biosystems, Foster City, CA) in triplicate as described [ ].

    Sequencing:

    Article Title: Oxytocin, vasopressin and estrogen receptor gene expression in relation to social recognition in female mice
    Article Snippet: .. Q-PCR reactions were carried out using the Taqman detection system (ABI Prism® 7000 Sequence Detection System, Applied Biosystems, Foster City, CA) and the following conditions: 2 min at 50 °C, 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 1 min at 60 °C. ..

    Article Title: Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex
    Article Snippet: The transcript levels of GREB1, RERG, GPER, BCAR1, BCAR3, CDK6, ERBB2 and ABCG2 were investigated by real-time PCR using Taqman detection system (Applied Biosystems, Carlsbad, CA, USA). .. Cycle programme (95 °C for 10 min to activate polymerase followed by 40 cycles of 95 °C for 15 s and 60 °C for 1 min) was started on an Abi Prism 7000 Sequence Detection System (Applied Biosystems).

    Quantitative RT-PCR:

    Article Title: Oxytocin, vasopressin and estrogen receptor gene expression in relation to social recognition in female mice
    Article Snippet: For each gene, qRT-PCR was carried out in all brain regions, using Applied Biosystems' gene expression assays (Foster City, CA): ERα (Assay ID Mm00433149_m1), ERβ (Assay ID Mm00599819_m1), PR (Assay ID Mm00435628_m1), OT (Assay ID Mm01329577_g1), OTR (Assay ID Mm01182684_m1), AVP (Assay ID Mm00437761_g1), AVPR1a (Assay ID Mm00444092_m1), and AVPR1b (Assay ID Mm01700416_m1). .. Q-PCR reactions were carried out using the Taqman detection system (ABI Prism® 7000 Sequence Detection System, Applied Biosystems, Foster City, CA) and the following conditions: 2 min at 50 °C, 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 1 min at 60 °C.

    Article Title: Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †
    Article Snippet: .. Validation of Differentially Expressed MiRNAs in Offspring In order to validate the expressions of differential miRNAs, quantitative real-time PCR (qRT-PCR) were utilized to detect the relative expression of differentially-expressed miRNAs, with samples enlarged in each group (n = 6 to 8 per group) using the TaqMan detection system (Life Technologies, Foster City, CA, USA). .. Total RNA was reversely transcribed using the TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, Life Technologies, Foster City, CA, USA).

    Article Title: Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex
    Article Snippet: Paragraph title: Quantitative RT-PCR ... The transcript levels of GREB1, RERG, GPER, BCAR1, BCAR3, CDK6, ERBB2 and ABCG2 were investigated by real-time PCR using Taqman detection system (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: Aldehyde dehydrogenase-1a1 induces oncogene suppressor genes in B cell populations
    Article Snippet: .. Total RNA was prepared using the RNeasy kit (Qiagen, Valencia, CA). qRT-PCR was performed with predesigned assays (Applied Biosystems, Foster City, CA) using on a 7900HT Fast Real-Time PCR System, TaqMan detection system, and validated primers (Applied Biosystems, Foster City, CA) in triplicate as described [ ]. .. The mRNA expression was calculated based on Tata-box binding protein (TBP) expression for normalization using the comparative Ct method.

    Electrophoresis:

    Article Title: Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157
    Article Snippet: At the same time, the ethidium bromide used to stain the electrophoresis gel after PCR is a harmful chemical and its application is time-consuming. .. The TaqMan detection system (Applied Biosystems, Foster City, Calif.) is a new qualitative and quantitative system that uses a fluorogenic hybridization probe to detect the target genes; and it has previously been demonstrated to be a rapid, high-throughput, semiautomatic PCR scheme for the identification of E. coli ( , ), Salmonella , and Listeria spp. ( ).

    Recombinant:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA). ..

    Concentration Assay:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex
    Article Snippet: The total RNA concentration was measured using a NanoDrop Fluorospectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The transcript levels of GREB1, RERG, GPER, BCAR1, BCAR3, CDK6, ERBB2 and ABCG2 were investigated by real-time PCR using Taqman detection system (Applied Biosystems, Carlsbad, CA, USA).

    High Throughput Screening Assay:

    Article Title: Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157
    Article Snippet: .. The TaqMan detection system (Applied Biosystems, Foster City, Calif.) is a new qualitative and quantitative system that uses a fluorogenic hybridization probe to detect the target genes; and it has previously been demonstrated to be a rapid, high-throughput, semiautomatic PCR scheme for the identification of E. coli ( , ), Salmonella , and Listeria spp. ( ). .. The objective of this study was to assess the utility of the TaqMan PCR system for the detection and identification of the stx 2 gene (which is responsible for the biosynthesis of Shiga-like toxin 2) and the rfbE. coli O157 gene (which is responsible for the biosynthesis of the O antigen) ( ).

    Staining:

    Article Title: Use of the Duplex TaqMan PCR System for Detection of Shiga-Like Toxin-Producing Escherichia coli O157
    Article Snippet: At the same time, the ethidium bromide used to stain the electrophoresis gel after PCR is a harmful chemical and its application is time-consuming. .. The TaqMan detection system (Applied Biosystems, Foster City, Calif.) is a new qualitative and quantitative system that uses a fluorogenic hybridization probe to detect the target genes; and it has previously been demonstrated to be a rapid, high-throughput, semiautomatic PCR scheme for the identification of E. coli ( , ), Salmonella , and Listeria spp. ( ).

    Control Assay:

    Article Title: Oxytocin, vasopressin and estrogen receptor gene expression in relation to social recognition in female mice
    Article Snippet: Eukaryotic 18S was used as an endogenous control (Assay ID Mm03928990_g1). .. Q-PCR reactions were carried out using the Taqman detection system (ABI Prism® 7000 Sequence Detection System, Applied Biosystems, Foster City, CA) and the following conditions: 2 min at 50 °C, 10 min at 95 °C, followed by 40 cycles of 15 s at 95 °C and 1 min at 60 °C.

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    Thermo Fisher mutation dectection castpcr idh1 hs00001019 rf
    Results of quantitative polymerase chain reaction: Wild-type IDH mRNA was clearly detected in the specimens of the first and last (sixth) surgeries, and in the autopsy specimens (left column). The mutated <t>IDH1</t> mRNA was consistently detected from the first surgical specimen onwards. The amplification was marginal but detectable in the last surgical specimen. However, in the autopsy specimen, mutated IDH mRNA could not be detected (right column, bottom).
    Mutation Dectection Castpcr Idh1 Hs00001019 Rf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mutation dectection castpcr idh1 hs00001019 rf/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mutation dectection castpcr idh1 hs00001019 rf - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    78
    Thermo Fisher mutation dectection castpcr idh1 hs00000981 mu
    Results of quantitative polymerase chain reaction: Wild-type IDH mRNA was clearly detected in the specimens of the first and last (sixth) surgeries, and in the autopsy specimens (left column). The mutated <t>IDH1</t> mRNA was consistently detected from the first surgical specimen onwards. The amplification was marginal but detectable in the last surgical specimen. However, in the autopsy specimen, mutated IDH mRNA could not be detected (right column, bottom).
    Mutation Dectection Castpcr Idh1 Hs00000981 Mu, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mutation dectection castpcr idh1 hs00000981 mu/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mutation dectection castpcr idh1 hs00000981 mu - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    78
    Thermo Fisher gene exp stat1 rn00583505 m1
    Quantitative analysis of induction of IRF1 , IRF2 , IRF9/ISGF3G , and <t>STAT1</t> mRNA in OPC (left column, OPC) and MO (right column) after the addition of IFNG (100 ng/ml, closed circle), IFNB (1000 U/ml, closed triangle), or medium alone (Control, open circle). Note that the data are plotted as ratios to copy numbers of GAPDH cDNA on a logarithmic scale. At time 0, data from controls are only shown. Statistical significance of transcriptional up-regulation of IRF1 and IRF9 at 24 h after the treatments was examined with at least 3 independent sets of experiments (far right graph). ** indicates P
    Gene Exp Stat1 Rn00583505 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp stat1 rn00583505 m1/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp stat1 rn00583505 m1 - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    Image Search Results


    Results of quantitative polymerase chain reaction: Wild-type IDH mRNA was clearly detected in the specimens of the first and last (sixth) surgeries, and in the autopsy specimens (left column). The mutated IDH1 mRNA was consistently detected from the first surgical specimen onwards. The amplification was marginal but detectable in the last surgical specimen. However, in the autopsy specimen, mutated IDH mRNA could not be detected (right column, bottom).

    Journal: Molecular and Clinical Oncology

    Article Title: Anaplastic astrocytoma cells not detectable on autopsy following long-term temozolomide treatment: A case report

    doi: 10.3892/mco.2017.1160

    Figure Lengend Snippet: Results of quantitative polymerase chain reaction: Wild-type IDH mRNA was clearly detected in the specimens of the first and last (sixth) surgeries, and in the autopsy specimens (left column). The mutated IDH1 mRNA was consistently detected from the first surgical specimen onwards. The amplification was marginal but detectable in the last surgical specimen. However, in the autopsy specimen, mutated IDH mRNA could not be detected (right column, bottom).

    Article Snippet: The primer sets were as follows: GAPDH: Hs99999905_m1 GAPDH (TaqMan Gene Expression Assays); wild-type IDH1: Hs00001019_rf IDH1_rf (TaqMan Mutation Detection Assays); and mutant IDH1 (R132H): Hs00000981_mu IDH1_28746_mu (TaqMan Mutation Detection Assays).

    Techniques: Real-time Polymerase Chain Reaction, Amplification

    Hemangioma sample obtained during the sixth surgery exhibiting (A) a connective tissue capsule and (B) sinusoidal vessels. In the autopsy specimens of the left frontal periventricular zone, GFAP-positive astrocytes were detected, but the cells were negative for p53 and IDH1 (C-F). H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1; GFAP, glial fibrillary acidic protein.

    Journal: Molecular and Clinical Oncology

    Article Title: Anaplastic astrocytoma cells not detectable on autopsy following long-term temozolomide treatment: A case report

    doi: 10.3892/mco.2017.1160

    Figure Lengend Snippet: Hemangioma sample obtained during the sixth surgery exhibiting (A) a connective tissue capsule and (B) sinusoidal vessels. In the autopsy specimens of the left frontal periventricular zone, GFAP-positive astrocytes were detected, but the cells were negative for p53 and IDH1 (C-F). H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1; GFAP, glial fibrillary acidic protein.

    Article Snippet: The primer sets were as follows: GAPDH: Hs99999905_m1 GAPDH (TaqMan Gene Expression Assays); wild-type IDH1: Hs00001019_rf IDH1_rf (TaqMan Mutation Detection Assays); and mutant IDH1 (R132H): Hs00000981_mu IDH1_28746_mu (TaqMan Mutation Detection Assays).

    Techniques:

    Changes in histopathological characteristics from the first surgery (row 1) to the sixth surgery (row 6). p53 and IDH1 were positive in the specimens obtained from all 6 surgeries. H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1.

    Journal: Molecular and Clinical Oncology

    Article Title: Anaplastic astrocytoma cells not detectable on autopsy following long-term temozolomide treatment: A case report

    doi: 10.3892/mco.2017.1160

    Figure Lengend Snippet: Changes in histopathological characteristics from the first surgery (row 1) to the sixth surgery (row 6). p53 and IDH1 were positive in the specimens obtained from all 6 surgeries. H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1.

    Article Snippet: The primer sets were as follows: GAPDH: Hs99999905_m1 GAPDH (TaqMan Gene Expression Assays); wild-type IDH1: Hs00001019_rf IDH1_rf (TaqMan Mutation Detection Assays); and mutant IDH1 (R132H): Hs00000981_mu IDH1_28746_mu (TaqMan Mutation Detection Assays).

    Techniques:

    Results of quantitative polymerase chain reaction: Wild-type IDH mRNA was clearly detected in the specimens of the first and last (sixth) surgeries, and in the autopsy specimens (left column). The mutated IDH1 mRNA was consistently detected from the first surgical specimen onwards. The amplification was marginal but detectable in the last surgical specimen. However, in the autopsy specimen, mutated IDH mRNA could not be detected (right column, bottom).

    Journal: Molecular and Clinical Oncology

    Article Title: Anaplastic astrocytoma cells not detectable on autopsy following long-term temozolomide treatment: A case report

    doi: 10.3892/mco.2017.1160

    Figure Lengend Snippet: Results of quantitative polymerase chain reaction: Wild-type IDH mRNA was clearly detected in the specimens of the first and last (sixth) surgeries, and in the autopsy specimens (left column). The mutated IDH1 mRNA was consistently detected from the first surgical specimen onwards. The amplification was marginal but detectable in the last surgical specimen. However, in the autopsy specimen, mutated IDH mRNA could not be detected (right column, bottom).

    Article Snippet: The primer sets were as follows: GAPDH: Hs99999905_m1 GAPDH (TaqMan Gene Expression Assays); wild-type IDH1: Hs00001019_rf IDH1_rf (TaqMan Mutation Detection Assays); and mutant IDH1 (R132H): Hs00000981_mu IDH1_28746_mu (TaqMan Mutation Detection Assays).

    Techniques: Real-time Polymerase Chain Reaction, Amplification

    Hemangioma sample obtained during the sixth surgery exhibiting (A) a connective tissue capsule and (B) sinusoidal vessels. In the autopsy specimens of the left frontal periventricular zone, GFAP-positive astrocytes were detected, but the cells were negative for p53 and IDH1 (C-F). H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1; GFAP, glial fibrillary acidic protein.

    Journal: Molecular and Clinical Oncology

    Article Title: Anaplastic astrocytoma cells not detectable on autopsy following long-term temozolomide treatment: A case report

    doi: 10.3892/mco.2017.1160

    Figure Lengend Snippet: Hemangioma sample obtained during the sixth surgery exhibiting (A) a connective tissue capsule and (B) sinusoidal vessels. In the autopsy specimens of the left frontal periventricular zone, GFAP-positive astrocytes were detected, but the cells were negative for p53 and IDH1 (C-F). H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1; GFAP, glial fibrillary acidic protein.

    Article Snippet: The primer sets were as follows: GAPDH: Hs99999905_m1 GAPDH (TaqMan Gene Expression Assays); wild-type IDH1: Hs00001019_rf IDH1_rf (TaqMan Mutation Detection Assays); and mutant IDH1 (R132H): Hs00000981_mu IDH1_28746_mu (TaqMan Mutation Detection Assays).

    Techniques:

    Changes in histopathological characteristics from the first surgery (row 1) to the sixth surgery (row 6). p53 and IDH1 were positive in the specimens obtained from all 6 surgeries. H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1.

    Journal: Molecular and Clinical Oncology

    Article Title: Anaplastic astrocytoma cells not detectable on autopsy following long-term temozolomide treatment: A case report

    doi: 10.3892/mco.2017.1160

    Figure Lengend Snippet: Changes in histopathological characteristics from the first surgery (row 1) to the sixth surgery (row 6). p53 and IDH1 were positive in the specimens obtained from all 6 surgeries. H E, hematoxylin and eosin; IDH1, isocitrate dehydrogenase 1.

    Article Snippet: The primer sets were as follows: GAPDH: Hs99999905_m1 GAPDH (TaqMan Gene Expression Assays); wild-type IDH1: Hs00001019_rf IDH1_rf (TaqMan Mutation Detection Assays); and mutant IDH1 (R132H): Hs00000981_mu IDH1_28746_mu (TaqMan Mutation Detection Assays).

    Techniques:

    Quantitative analysis of induction of IRF1 , IRF2 , IRF9/ISGF3G , and STAT1 mRNA in OPC (left column, OPC) and MO (right column) after the addition of IFNG (100 ng/ml, closed circle), IFNB (1000 U/ml, closed triangle), or medium alone (Control, open circle). Note that the data are plotted as ratios to copy numbers of GAPDH cDNA on a logarithmic scale. At time 0, data from controls are only shown. Statistical significance of transcriptional up-regulation of IRF1 and IRF9 at 24 h after the treatments was examined with at least 3 independent sets of experiments (far right graph). ** indicates P

    Journal: Journal of neuroimmunology

    Article Title: Interferon-triggered transcriptional cascades in the oligodendroglial lineage: a comparison of induction of MHC class II antigen between oligodendroglial progenitor cells and mature oligodendrocytes

    doi: 10.1016/j.jneuroim.2009.04.021

    Figure Lengend Snippet: Quantitative analysis of induction of IRF1 , IRF2 , IRF9/ISGF3G , and STAT1 mRNA in OPC (left column, OPC) and MO (right column) after the addition of IFNG (100 ng/ml, closed circle), IFNB (1000 U/ml, closed triangle), or medium alone (Control, open circle). Note that the data are plotted as ratios to copy numbers of GAPDH cDNA on a logarithmic scale. At time 0, data from controls are only shown. Statistical significance of transcriptional up-regulation of IRF1 and IRF9 at 24 h after the treatments was examined with at least 3 independent sets of experiments (far right graph). ** indicates P

    Article Snippet: Quantitative PCR (qPCR) analysis was performed by MX3005P (Stratagene, La Jolla, CA) using TaqMan® Assay-on-Demand™ assay kits (assay numbers: Rn00583505_m1, Rn00561424_m1, Rn00585451_m1, Rn01427980_m1, Rn00565062_m1, Rn00709368_m1 and Rn00571654_m1 for detection of STAT1 , IRF1 , CIITA , RT1DA , CD74 , CD80 and CD86 , respectively) (Foster City, CA).

    Techniques:

    A and B : Time-course of phosphorylation of STAT1, and up-regulation of STAT1 and IRF1 proteins in OPC in response to IFNG ( A ) or IFNB ( B ). Protein aliquots (20 μg) from OPC incubated with IFNG (100 ng/ml), IFNB (1000 U/ml) or both (far right lanes in A and B) for the indicated time-periods were immunoblotted for phosphorylated STAT1, total STAT1, and IRF1. The two arrowheads in the phosphorylated STAT1 and total STAT1 panels indicate the two alternatively spliced forms, STAT1α (91 kDa, top) and STAT1β (84 kDa, bottom). Note that the faint signals of IRF1 in IFNB-treated OPCs from 1 h to 24 h with a peak induction at 3 h. C : IRF1 induction by the interferons in comparison between OPC and MO. Protein aliquots (20 μg) from OPC and MO treated with IFNG (G, 100 ng/ml), IFNB (B, 1000 U/ml) or the culture medium alone (C) for 24 h were immunoblotted for IRF1. IRF1 protein was robustly induced by IFNG in both OPC and MO, but only faintly by IFNB in OPC. The subsequent immunoblot for GAPDH is shown for equal protein loading.

    Journal: Journal of neuroimmunology

    Article Title: Interferon-triggered transcriptional cascades in the oligodendroglial lineage: a comparison of induction of MHC class II antigen between oligodendroglial progenitor cells and mature oligodendrocytes

    doi: 10.1016/j.jneuroim.2009.04.021

    Figure Lengend Snippet: A and B : Time-course of phosphorylation of STAT1, and up-regulation of STAT1 and IRF1 proteins in OPC in response to IFNG ( A ) or IFNB ( B ). Protein aliquots (20 μg) from OPC incubated with IFNG (100 ng/ml), IFNB (1000 U/ml) or both (far right lanes in A and B) for the indicated time-periods were immunoblotted for phosphorylated STAT1, total STAT1, and IRF1. The two arrowheads in the phosphorylated STAT1 and total STAT1 panels indicate the two alternatively spliced forms, STAT1α (91 kDa, top) and STAT1β (84 kDa, bottom). Note that the faint signals of IRF1 in IFNB-treated OPCs from 1 h to 24 h with a peak induction at 3 h. C : IRF1 induction by the interferons in comparison between OPC and MO. Protein aliquots (20 μg) from OPC and MO treated with IFNG (G, 100 ng/ml), IFNB (B, 1000 U/ml) or the culture medium alone (C) for 24 h were immunoblotted for IRF1. IRF1 protein was robustly induced by IFNG in both OPC and MO, but only faintly by IFNB in OPC. The subsequent immunoblot for GAPDH is shown for equal protein loading.

    Article Snippet: Quantitative PCR (qPCR) analysis was performed by MX3005P (Stratagene, La Jolla, CA) using TaqMan® Assay-on-Demand™ assay kits (assay numbers: Rn00583505_m1, Rn00561424_m1, Rn00585451_m1, Rn01427980_m1, Rn00565062_m1, Rn00709368_m1 and Rn00571654_m1 for detection of STAT1 , IRF1 , CIITA , RT1DA , CD74 , CD80 and CD86 , respectively) (Foster City, CA).

    Techniques: Incubation