taq polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher taq polymerase
    Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq polymerase/product/Thermo Fisher
    Average 99 stars, based on 2955 article reviews
    Price from $9.99 to $1999.99
    taq polymerase - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: Primers were designed to have XhoI and NdeI restriction sites for insertion into the pET28a-TEV and pET30a cloning vectors. .. Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen).

    Amplification:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: The fragment amplified with the Cj0485NdeIF primer and Cj0485XhoIR primer with the stop codon intact was ligated overnight at room temperature using T4 DNA ligase as per manufacturer’s protocol into Xho/NdeI digested pET28a-TEV to create an N-terminally-His-tagged construct. .. Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen).

    Article Title: Henneguya (Cnidaria: Myxosporea: Myxobolidae) infections of cultured barramundi, Lates calcarifer (Perciformes: Latidae) in an estuarine wetlands system of Malaysia: description of Henneguya setiuensis n. sp., Henneguya voronini n. sp. and Henneguya calcarifer n. sp.
    Article Snippet: .. Second-round PCR was conducted in 50 μl, which consisted of 1 μl amplified DNA ( ~ 0.5 μg), 0.2 mM of each dNTP (MBI Fermentas), 0.5 μM each of the forward and reverse primers, 5 μl 10 × Taq buffer (MBI Fermentas), 2 U Taq polymerase (MBI Fermentas) and 33 μl of water. .. All PCRs were performed in a PTC-200 thermocycler (MJ Research).

    Article Title: Glacial Runoff Promotes Deep Burial of Sulfur Cycling-Associated Microorganisms in Marine Sediments
    Article Snippet: Paragraph title: DNA Extraction and Preparation of 16S rRNA Gene and dsrB Amplicon Libraries ... The reaction mix contained 1 × Taq buffer (Thermo Scientific), 0.2 mM dNTP mix (Thermo Scientific), 2 mM MgCl2 , 0.25 U Taq polymerase (Thermo Scientific), 0.2 μM of each primer, and approximately 1−10 ng DNA.

    Article Title: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
    Article Snippet: .. A 919 bp product spanning the prnp coding region was amplified from each elk sample in a 100 μl reaction using the following conditions (final concentration in brackets): 2.5 U Taq polymerase (Invitrogen), 10x buffer (1×), 25 mM dNTPs (2 mM), 50 mM MgCl2 (2.5 mM), 5 μM forward primer (0.5 μM), 5 μM reverse primer (0.5 μM), 100 ng of DNA (1 ng/μl). .. The PCR conditions were as follows: 5 minute denaturing phase at 94 °C, followed by 36 cycles of: 1 minute denaturing at 94 °C; 30 seconds annealing at 64.5 °C; 1 minute extension at 72 °C; with a final extension step of 72 °C for 10 minutes.

    Article Title: Evolution of Hepatitis B Virus Receptor NTCP Reveals Differential Pathogenicities and Species Specificities of Hepadnaviruses in Primates, Rodents, and Bats
    Article Snippet: To ensure proper identification of the sampled bat species, we amplified and sequenced the mitochondrial gene cytochrome b (Cytb), using the primers CytB-F and CytB-L/R ( ). .. PCRs were carried out in a total volume of 25 μl containing 1× reaction buffer (DreamTaq DNA polymerase; Thermo Fisher Scientific), 0.2 mM each deoxynucleoside triphosphate (dNTP), 0.2 μM each primer, 1 U of Taq polymerase (DreamTaq DNA polymerase; Thermo Fisher Scientific), and approximately 10 ng of extracted genomic DNA.

    Article Title: The molecular and cellular basis of exostosis formation in hereditary multiple exostoses
    Article Snippet: The cDNA products were used directly as templates for PCR amplification. .. PCR was performed in a final volume of 25 μl containing 10× PCR buffer [1x = 20 m m Tris-HCl (pH 8.4), 50 m m KCl], 1.5 m m MgCl2 , 1.25 U Taq DNA Polymerase (Gibco BRL®), 0.1 m m dNTPs (Gibco BRL®), 10 μ m each of the primers and 10 ng/μl template cDNA.

    Article Title: Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas
    Article Snippet: .. For B2L gene amplification, PCR was performed in a thermocycler using 2 µl of DNA as template in a 25 μl reaction volume containing 10 pmol of each primer and 1 U Taq DNA polymerase (Fermentas, USA). .. The thermal profile used for the PCR was to heat samples to 94 °C for 3 min, followed by 30 cycles of 1 min at 94 °C, 1 min at 52 °C and 1 min at 72 °C, with the final extension at 72 °C for 7 min. Of total 36 scab samples screened, 31 (86.11%) came positive for B2L gene amplification (Fig. c).

    Synthesized:

    Article Title: Homeostasis of Retinol in Lecithin:retinol Acyltransferase Gene Knockout Mice Fed a High Retinol Diet
    Article Snippet: The synthesized cDNA was diluted to 100 µl with water, and 1 µl of each diluted cDNA sample was used in the PCR. .. The PCR was performed using the following conditions: 94 °C for 30 s, 58 °C for 30 s, and 72 °C for 80 s, with a final extension at 72 °C for 10 min. Taq polymerase was from Invitrogen (catalog number 18038–042).

    Construct:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: .. Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen). .. FucX protein purification The pET expression constructs described in the previous section were isolated from DH5α using the GeneJET Plasmid Miniprep kit (Thermo Scientific) per manufacturer’s instructions, modified to include 5 min drying prior to eluting with 30 µL 1/30 elution buffer pre-warmed to 55 °C and incubating for 15 min while eluting prior to spinning down.

    Real-time Polymerase Chain Reaction:

    Article Title: Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds
    Article Snippet: The QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) was used to perform quantitative real-time PCR. .. Taq polymerase and Taqman primers (Thermo Fisher Scientific) glyceraldehyde-3-phosphate dehydrogenase (GAPDH Hs02786624_g1), neural cell adhesion molecule 1 (NCAM1 Hs00941830_m1), heparin binding EGF like growth factor (HBEGF Hs00181813_m1), heat shock protein family member 1 (HSPB1 Hs00356629_g1), enolase 2 (ENO2 Hs00157360_m1), vascular endothelial growth factor A (VEGF-A Hs00900055_m1), glial cell derived neurotrophic factor (GDNF Hs01931883_s1), brain derived neurotrophic factor (BDNF Hs02718934_s1), and neurotrophin 3 (NTF3 Hs00267375_s1) for formed the PCR reaction mixtures.

    Article Title: Homeostasis of Retinol in Lecithin:retinol Acyltransferase Gene Knockout Mice Fed a High Retinol Diet
    Article Snippet: Paragraph title: 3.3 Semi-quantitative RT-PCR and quantitative real time PCR ... The PCR was performed using the following conditions: 94 °C for 30 s, 58 °C for 30 s, and 72 °C for 80 s, with a final extension at 72 °C for 10 min. Taq polymerase was from Invitrogen (catalog number 18038–042).

    Incubation:

    Article Title: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
    Article Snippet: DNA was extracted from the buffy coat as follows: 100–200 μl of buffy coat was incubated at 60 °C overnight in a solution of Proteinase K (80 μl) and tissue lysis buffer (450 μl). .. A 919 bp product spanning the prnp coding region was amplified from each elk sample in a 100 μl reaction using the following conditions (final concentration in brackets): 2.5 U Taq polymerase (Invitrogen), 10x buffer (1×), 25 mM dNTPs (2 mM), 50 mM MgCl2 (2.5 mM), 5 μM forward primer (0.5 μM), 5 μM reverse primer (0.5 μM), 100 ng of DNA (1 ng/μl).

    Expressing:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: Paragraph title: Protein expression constructs ... Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen).

    Article Title: Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds
    Article Snippet: Paragraph title: Quantitative gene expression ... Taq polymerase and Taqman primers (Thermo Fisher Scientific) glyceraldehyde-3-phosphate dehydrogenase (GAPDH Hs02786624_g1), neural cell adhesion molecule 1 (NCAM1 Hs00941830_m1), heparin binding EGF like growth factor (HBEGF Hs00181813_m1), heat shock protein family member 1 (HSPB1 Hs00356629_g1), enolase 2 (ENO2 Hs00157360_m1), vascular endothelial growth factor A (VEGF-A Hs00900055_m1), glial cell derived neurotrophic factor (GDNF Hs01931883_s1), brain derived neurotrophic factor (BDNF Hs02718934_s1), and neurotrophin 3 (NTF3 Hs00267375_s1) for formed the PCR reaction mixtures.

    Article Title: Generation of transgene-free porcine intermediate type induced pluripotent stem cells
    Article Snippet: For assessment of exogenous and endogenous gene expression, piPSCs from the four cell lines were harvested at both passage 10 and passage 20 and lysed in 350 µl RLT buffer (Qiagen) containing β-mercaptoethanol (Sigma-Aldrich). .. First-strand cDNA was used as template for subsequent PCR analysis in a 50 µl total volume composed of 5 µl 10x Taq buffer (Fermentas/Thermo Scientific), 2 µl 25 mM MgCl2 (Fermentas/Thermo Scientific), 2 µl 10mM deoxyribonucleotide triphosphates (dNTPs; Fermentas/Thermo Scientific), and 1 µl 5U/µL Taq polymerase (Fermentas/Thermo Scientific) in RNase-free water.

    Modification:

    Article Title: Glacial Runoff Promotes Deep Burial of Sulfur Cycling-Associated Microorganisms in Marine Sediments
    Article Snippet: These primers were modified with a 16 bp head sequence as described previously ( ). .. The reaction mix contained 1 × Taq buffer (Thermo Scientific), 0.2 mM dNTP mix (Thermo Scientific), 2 mM MgCl2 , 0.25 U Taq polymerase (Thermo Scientific), 0.2 μM of each primer, and approximately 1−10 ng DNA.

    Transformation Assay:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: .. Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen). .. FucX protein purification The pET expression constructs described in the previous section were isolated from DH5α using the GeneJET Plasmid Miniprep kit (Thermo Scientific) per manufacturer’s instructions, modified to include 5 min drying prior to eluting with 30 µL 1/30 elution buffer pre-warmed to 55 °C and incubating for 15 min while eluting prior to spinning down.

    Derivative Assay:

    Article Title: Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds
    Article Snippet: .. Taq polymerase and Taqman primers (Thermo Fisher Scientific) glyceraldehyde-3-phosphate dehydrogenase (GAPDH Hs02786624_g1), neural cell adhesion molecule 1 (NCAM1 Hs00941830_m1), heparin binding EGF like growth factor (HBEGF Hs00181813_m1), heat shock protein family member 1 (HSPB1 Hs00356629_g1), enolase 2 (ENO2 Hs00157360_m1), vascular endothelial growth factor A (VEGF-A Hs00900055_m1), glial cell derived neurotrophic factor (GDNF Hs01931883_s1), brain derived neurotrophic factor (BDNF Hs02718934_s1), and neurotrophin 3 (NTF3 Hs00267375_s1) for formed the PCR reaction mixtures. ..

    Ligation:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: .. Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen). .. FucX protein purification The pET expression constructs described in the previous section were isolated from DH5α using the GeneJET Plasmid Miniprep kit (Thermo Scientific) per manufacturer’s instructions, modified to include 5 min drying prior to eluting with 30 µL 1/30 elution buffer pre-warmed to 55 °C and incubating for 15 min while eluting prior to spinning down.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Homeostasis of Retinol in Lecithin:retinol Acyltransferase Gene Knockout Mice Fed a High Retinol Diet
    Article Snippet: Paragraph title: 3.3 Semi-quantitative RT-PCR and quantitative real time PCR ... The PCR was performed using the following conditions: 94 °C for 30 s, 58 °C for 30 s, and 72 °C for 80 s, with a final extension at 72 °C for 10 min. Taq polymerase was from Invitrogen (catalog number 18038–042).

    Article Title: The molecular and cellular basis of exostosis formation in hereditary multiple exostoses
    Article Snippet: Paragraph title: RNA isolation and RT-PCR analysis of EXT1 mRNA ... PCR was performed in a final volume of 25 μl containing 10× PCR buffer [1x = 20 m m Tris-HCl (pH 8.4), 50 m m KCl], 1.5 m m MgCl2 , 1.25 U Taq DNA Polymerase (Gibco BRL®), 0.1 m m dNTPs (Gibco BRL®), 10 μ m each of the primers and 10 ng/μl template cDNA.

    Article Title: Generation of transgene-free porcine intermediate type induced pluripotent stem cells
    Article Snippet: Paragraph title: RNA extraction and reverse transcription PCR (RT-PCR) ... First-strand cDNA was used as template for subsequent PCR analysis in a 50 µl total volume composed of 5 µl 10x Taq buffer (Fermentas/Thermo Scientific), 2 µl 25 mM MgCl2 (Fermentas/Thermo Scientific), 2 µl 10mM deoxyribonucleotide triphosphates (dNTPs; Fermentas/Thermo Scientific), and 1 µl 5U/µL Taq polymerase (Fermentas/Thermo Scientific) in RNase-free water.

    Polymerase Chain Reaction:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: .. Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen). .. FucX protein purification The pET expression constructs described in the previous section were isolated from DH5α using the GeneJET Plasmid Miniprep kit (Thermo Scientific) per manufacturer’s instructions, modified to include 5 min drying prior to eluting with 30 µL 1/30 elution buffer pre-warmed to 55 °C and incubating for 15 min while eluting prior to spinning down.

    Article Title: Glial Fibrillary Acidic Protein–Apolipoprotein E (apoE) Transgenic Mice: Astrocyte-Specific Expression and Differing Biological Effects of Astrocyte-Secreted apoE3 and apoE4 Lipoproteins
    Article Snippet: .. The PCR reaction was in 50 μl and contained 2 μl of DNA (∼50 ng), 2.5 μl of each primer (10 μ m stock solution), 10× PCR buffer (20 m m Tris-HCl, pH 8.0, 0.1 m m EDTA, 1 m m DTT, and 50% glycerol), 200 μ m dNTP, and 0.25 μl of Taq polymerase (5 U/μl; Life Technologies, Grand Island, NY). .. The reaction was performed at 94°C for 3 min, followed by 35 cycles at 94°C for 45 sec, 65°C for 40 sec, and 72°C for 1 min, and then the reaction was completed at 72°C for 10 min.

    Article Title: Henneguya (Cnidaria: Myxosporea: Myxobolidae) infections of cultured barramundi, Lates calcarifer (Perciformes: Latidae) in an estuarine wetlands system of Malaysia: description of Henneguya setiuensis n. sp., Henneguya voronini n. sp. and Henneguya calcarifer n. sp.
    Article Snippet: .. Second-round PCR was conducted in 50 μl, which consisted of 1 μl amplified DNA ( ~ 0.5 μg), 0.2 mM of each dNTP (MBI Fermentas), 0.5 μM each of the forward and reverse primers, 5 μl 10 × Taq buffer (MBI Fermentas), 2 U Taq polymerase (MBI Fermentas) and 33 μl of water. .. All PCRs were performed in a PTC-200 thermocycler (MJ Research).

    Article Title: Glacial Runoff Promotes Deep Burial of Sulfur Cycling-Associated Microorganisms in Marine Sediments
    Article Snippet: Barcoded 16S rRNA gene amplicons were produced with a two-step PCR barcoding approach , using the general bacterial and archaeal primers U519F (5′-CAGCMGCCGCGGTAATWC-3′) and 802R (5′-TACNVGGGTATCTAATCC-3′) for initial amplification ( ). .. The reaction mix contained 1 × Taq buffer (Thermo Scientific), 0.2 mM dNTP mix (Thermo Scientific), 2 mM MgCl2 , 0.25 U Taq polymerase (Thermo Scientific), 0.2 μM of each primer, and approximately 1−10 ng DNA.

    Article Title: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
    Article Snippet: A 919 bp product spanning the prnp coding region was amplified from each elk sample in a 100 μl reaction using the following conditions (final concentration in brackets): 2.5 U Taq polymerase (Invitrogen), 10x buffer (1×), 25 mM dNTPs (2 mM), 50 mM MgCl2 (2.5 mM), 5 μM forward primer (0.5 μM), 5 μM reverse primer (0.5 μM), 100 ng of DNA (1 ng/μl). .. The PCR conditions were as follows: 5 minute denaturing phase at 94 °C, followed by 36 cycles of: 1 minute denaturing at 94 °C; 30 seconds annealing at 64.5 °C; 1 minute extension at 72 °C; with a final extension step of 72 °C for 10 minutes.

    Article Title: Homeostasis of Retinol in Lecithin:retinol Acyltransferase Gene Knockout Mice Fed a High Retinol Diet
    Article Snippet: .. The PCR was performed using the following conditions: 94 °C for 30 s, 58 °C for 30 s, and 72 °C for 80 s, with a final extension at 72 °C for 10 min. Taq polymerase was from Invitrogen (catalog number 18038–042). ..

    Article Title: Evolution of Hepatitis B Virus Receptor NTCP Reveals Differential Pathogenicities and Species Specificities of Hepadnaviruses in Primates, Rodents, and Bats
    Article Snippet: PCRs were carried out in a total volume of 25 μl containing 1× reaction buffer (DreamTaq DNA polymerase; Thermo Fisher Scientific), 0.2 mM each deoxynucleoside triphosphate (dNTP), 0.2 μM each primer, 1 U of Taq polymerase (DreamTaq DNA polymerase; Thermo Fisher Scientific), and approximately 10 ng of extracted genomic DNA. .. PCR products with multiple bands were excised and purified from gel using the NucleoSpin gel and PCR clean-up kit from Macherey-Nagel.

    Article Title: The molecular and cellular basis of exostosis formation in hereditary multiple exostoses
    Article Snippet: .. PCR was performed in a final volume of 25 μl containing 10× PCR buffer [1x = 20 m m Tris-HCl (pH 8.4), 50 m m KCl], 1.5 m m MgCl2 , 1.25 U Taq DNA Polymerase (Gibco BRL®), 0.1 m m dNTPs (Gibco BRL®), 10 μ m each of the primers and 10 ng/μl template cDNA. ..

    Article Title: Generation of transgene-free porcine intermediate type induced pluripotent stem cells
    Article Snippet: .. First-strand cDNA was used as template for subsequent PCR analysis in a 50 µl total volume composed of 5 µl 10x Taq buffer (Fermentas/Thermo Scientific), 2 µl 25 mM MgCl2 (Fermentas/Thermo Scientific), 2 µl 10mM deoxyribonucleotide triphosphates (dNTPs; Fermentas/Thermo Scientific), and 1 µl 5U/µL Taq polymerase (Fermentas/Thermo Scientific) in RNase-free water. .. Plasmid specific primers as described by Okita et al., 2011 [ ] were used for RT-PCR ( ).

    Article Title: Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas
    Article Snippet: .. For B2L gene amplification, PCR was performed in a thermocycler using 2 µl of DNA as template in a 25 μl reaction volume containing 10 pmol of each primer and 1 U Taq DNA polymerase (Fermentas, USA). .. The thermal profile used for the PCR was to heat samples to 94 °C for 3 min, followed by 30 cycles of 1 min at 94 °C, 1 min at 52 °C and 1 min at 72 °C, with the final extension at 72 °C for 7 min. Of total 36 scab samples screened, 31 (86.11%) came positive for B2L gene amplification (Fig. c).

    Article Title: Molecular identification and chromosomal localization of new powdery mildew resistance gene Pm11 in oat
    Article Snippet: .. Specific PCR Reaction mixtures had a final volume of 10 μl consisting of 60 ng of total genomic DNA, 20–40 μM of each PCR primer (quantity was measured for each primer), 0.1 mM dNTPs, 1.5–2.5 mM MgCl2 (quantity was tested for each primer), 1 × reaction buffer and 0.5 U Taq Polymerase, Thermo Fisher. .. PCR was conducted in a T1Biometra thermocycler.

    Sequencing:

    Article Title: Henneguya (Cnidaria: Myxosporea: Myxobolidae) infections of cultured barramundi, Lates calcarifer (Perciformes: Latidae) in an estuarine wetlands system of Malaysia: description of Henneguya setiuensis n. sp., Henneguya voronini n. sp. and Henneguya calcarifer n. sp.
    Article Snippet: Second-round PCR was conducted in 50 μl, which consisted of 1 μl amplified DNA ( ~ 0.5 μg), 0.2 mM of each dNTP (MBI Fermentas), 0.5 μM each of the forward and reverse primers, 5 μl 10 × Taq buffer (MBI Fermentas), 2 U Taq polymerase (MBI Fermentas) and 33 μl of water. .. Amplified DNA was purified with EZ-10 Spin Column PCR Purification Kit (Bio Basic Inc., USA) and then sequenced in both directions using an ABI BigDye Terminator v3.1 Cycle Sequencing Kit with an ABI 3100 Genetic Analyser (IVMR, HAS).

    Article Title: Glacial Runoff Promotes Deep Burial of Sulfur Cycling-Associated Microorganisms in Marine Sediments
    Article Snippet: These primers were modified with a 16 bp head sequence as described previously ( ). .. The reaction mix contained 1 × Taq buffer (Thermo Scientific), 0.2 mM dNTP mix (Thermo Scientific), 2 mM MgCl2 , 0.25 U Taq polymerase (Thermo Scientific), 0.2 μM of each primer, and approximately 1−10 ng DNA.

    Article Title: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
    Article Snippet: A 919 bp product spanning the prnp coding region was amplified from each elk sample in a 100 μl reaction using the following conditions (final concentration in brackets): 2.5 U Taq polymerase (Invitrogen), 10x buffer (1×), 25 mM dNTPs (2 mM), 50 mM MgCl2 (2.5 mM), 5 μM forward primer (0.5 μM), 5 μM reverse primer (0.5 μM), 100 ng of DNA (1 ng/μl). .. Sequencing was performed by the DNA Core Facility of the National Microbiology Laboratory using an ABI 3730 DNA Analyzer, and Applied Biosystems BigDye Terminator Version 3.1 chemistry.

    Article Title: Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas
    Article Snippet: For B2L gene amplification, PCR was performed in a thermocycler using 2 µl of DNA as template in a 25 μl reaction volume containing 10 pmol of each primer and 1 U Taq DNA polymerase (Fermentas, USA). .. The low detection rate in our case may be attributed to the presence of PCR inhibitors in scab material, sequence divergence in the B2L gene or the larger size of the PCR amplicon.

    Binding Assay:

    Article Title: Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds
    Article Snippet: .. Taq polymerase and Taqman primers (Thermo Fisher Scientific) glyceraldehyde-3-phosphate dehydrogenase (GAPDH Hs02786624_g1), neural cell adhesion molecule 1 (NCAM1 Hs00941830_m1), heparin binding EGF like growth factor (HBEGF Hs00181813_m1), heat shock protein family member 1 (HSPB1 Hs00356629_g1), enolase 2 (ENO2 Hs00157360_m1), vascular endothelial growth factor A (VEGF-A Hs00900055_m1), glial cell derived neurotrophic factor (GDNF Hs01931883_s1), brain derived neurotrophic factor (BDNF Hs02718934_s1), and neurotrophin 3 (NTF3 Hs00267375_s1) for formed the PCR reaction mixtures. ..

    DNA Extraction:

    Article Title: Glacial Runoff Promotes Deep Burial of Sulfur Cycling-Associated Microorganisms in Marine Sediments
    Article Snippet: Paragraph title: DNA Extraction and Preparation of 16S rRNA Gene and dsrB Amplicon Libraries ... The reaction mix contained 1 × Taq buffer (Thermo Scientific), 0.2 mM dNTP mix (Thermo Scientific), 2 mM MgCl2 , 0.25 U Taq polymerase (Thermo Scientific), 0.2 μM of each primer, and approximately 1−10 ng DNA.

    Article Title: Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas
    Article Snippet: The homogenates were digested by proteinase K at 55 °C for 3 h and processed for DNA extraction using Wizard® Genomic DNA Purification Kit (Promega, USA) as instructed by the manufacturer. .. For B2L gene amplification, PCR was performed in a thermocycler using 2 µl of DNA as template in a 25 μl reaction volume containing 10 pmol of each primer and 1 U Taq DNA polymerase (Fermentas, USA).

    Isolation:

    Article Title: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
    Article Snippet: Elk Genotyping Buffy coat was isolated from 96 CWD-positive and 95 CWD-negative elk using a Ficoll-Paque gradient. .. A 919 bp product spanning the prnp coding region was amplified from each elk sample in a 100 μl reaction using the following conditions (final concentration in brackets): 2.5 U Taq polymerase (Invitrogen), 10x buffer (1×), 25 mM dNTPs (2 mM), 50 mM MgCl2 (2.5 mM), 5 μM forward primer (0.5 μM), 5 μM reverse primer (0.5 μM), 100 ng of DNA (1 ng/μl).

    Article Title: The molecular and cellular basis of exostosis formation in hereditary multiple exostoses
    Article Snippet: Paragraph title: RNA isolation and RT-PCR analysis of EXT1 mRNA ... PCR was performed in a final volume of 25 μl containing 10× PCR buffer [1x = 20 m m Tris-HCl (pH 8.4), 50 m m KCl], 1.5 m m MgCl2 , 1.25 U Taq DNA Polymerase (Gibco BRL®), 0.1 m m dNTPs (Gibco BRL®), 10 μ m each of the primers and 10 ng/μl template cDNA.

    Size-exclusion Chromatography:

    Article Title: Glial Fibrillary Acidic Protein–Apolipoprotein E (apoE) Transgenic Mice: Astrocyte-Specific Expression and Differing Biological Effects of Astrocyte-Secreted apoE3 and apoE4 Lipoproteins
    Article Snippet: The PCR reaction was in 50 μl and contained 2 μl of DNA (∼50 ng), 2.5 μl of each primer (10 μ m stock solution), 10× PCR buffer (20 m m Tris-HCl, pH 8.0, 0.1 m m EDTA, 1 m m DTT, and 50% glycerol), 200 μ m dNTP, and 0.25 μl of Taq polymerase (5 U/μl; Life Technologies, Grand Island, NY). .. The reaction was performed at 94°C for 3 min, followed by 35 cycles at 94°C for 45 sec, 65°C for 40 sec, and 72°C for 1 min, and then the reaction was completed at 72°C for 10 min.

    Purification:

    Article Title: The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus
    Article Snippet: Vectors and inserts were digested with XhoI (Promega) and NdeI (Thermo Fast Digest) following the ThermoScientific protocol for 75 min and purified using the Monarch PCR and DNA clean up kit (NEB) using the manufacturer’s protocol. .. Following ligation, constructs were transformed into E. coli DH5α and confirmed by colony PCR using Taq polymerase (Invitrogen).

    Article Title: Henneguya (Cnidaria: Myxosporea: Myxobolidae) infections of cultured barramundi, Lates calcarifer (Perciformes: Latidae) in an estuarine wetlands system of Malaysia: description of Henneguya setiuensis n. sp., Henneguya voronini n. sp. and Henneguya calcarifer n. sp.
    Article Snippet: Second-round PCR was conducted in 50 μl, which consisted of 1 μl amplified DNA ( ~ 0.5 μg), 0.2 mM of each dNTP (MBI Fermentas), 0.5 μM each of the forward and reverse primers, 5 μl 10 × Taq buffer (MBI Fermentas), 2 U Taq polymerase (MBI Fermentas) and 33 μl of water. .. Amplified DNA was purified with EZ-10 Spin Column PCR Purification Kit (Bio Basic Inc., USA) and then sequenced in both directions using an ABI BigDye Terminator v3.1 Cycle Sequencing Kit with an ABI 3100 Genetic Analyser (IVMR, HAS).

    Article Title: Evolution of Hepatitis B Virus Receptor NTCP Reveals Differential Pathogenicities and Species Specificities of Hepadnaviruses in Primates, Rodents, and Bats
    Article Snippet: PCRs were carried out in a total volume of 25 μl containing 1× reaction buffer (DreamTaq DNA polymerase; Thermo Fisher Scientific), 0.2 mM each deoxynucleoside triphosphate (dNTP), 0.2 μM each primer, 1 U of Taq polymerase (DreamTaq DNA polymerase; Thermo Fisher Scientific), and approximately 10 ng of extracted genomic DNA. .. PCR products with multiple bands were excised and purified from gel using the NucleoSpin gel and PCR clean-up kit from Macherey-Nagel.

    Transgenic Assay:

    Article Title: Glial Fibrillary Acidic Protein–Apolipoprotein E (apoE) Transgenic Mice: Astrocyte-Specific Expression and Differing Biological Effects of Astrocyte-Secreted apoE3 and apoE4 Lipoproteins
    Article Snippet: In addition, films were exposed to a phosphorimaging screen, and transgene copy number was determined by comparison of the signal obtained for the endogenous mouse (∼3 kb) and transgenic human apoE (∼1 kb) bands. .. The PCR reaction was in 50 μl and contained 2 μl of DNA (∼50 ng), 2.5 μl of each primer (10 μ m stock solution), 10× PCR buffer (20 m m Tris-HCl, pH 8.0, 0.1 m m EDTA, 1 m m DTT, and 50% glycerol), 200 μ m dNTP, and 0.25 μl of Taq polymerase (5 U/μl; Life Technologies, Grand Island, NY).

    Quantitative RT-PCR:

    Article Title: Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds
    Article Snippet: Quantitative gene expression The quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the RNeasy Mini Kit (Qiagen) based on the manufacturer’s protocol. .. Taq polymerase and Taqman primers (Thermo Fisher Scientific) glyceraldehyde-3-phosphate dehydrogenase (GAPDH Hs02786624_g1), neural cell adhesion molecule 1 (NCAM1 Hs00941830_m1), heparin binding EGF like growth factor (HBEGF Hs00181813_m1), heat shock protein family member 1 (HSPB1 Hs00356629_g1), enolase 2 (ENO2 Hs00157360_m1), vascular endothelial growth factor A (VEGF-A Hs00900055_m1), glial cell derived neurotrophic factor (GDNF Hs01931883_s1), brain derived neurotrophic factor (BDNF Hs02718934_s1), and neurotrophin 3 (NTF3 Hs00267375_s1) for formed the PCR reaction mixtures.

    Lysis:

    Article Title: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
    Article Snippet: DNA was extracted from the buffy coat as follows: 100–200 μl of buffy coat was incubated at 60 °C overnight in a solution of Proteinase K (80 μl) and tissue lysis buffer (450 μl). .. A 919 bp product spanning the prnp coding region was amplified from each elk sample in a 100 μl reaction using the following conditions (final concentration in brackets): 2.5 U Taq polymerase (Invitrogen), 10x buffer (1×), 25 mM dNTPs (2 mM), 50 mM MgCl2 (2.5 mM), 5 μM forward primer (0.5 μM), 5 μM reverse primer (0.5 μM), 100 ng of DNA (1 ng/μl).

    Plasmid Preparation:

    Article Title: Generation of transgene-free porcine intermediate type induced pluripotent stem cells
    Article Snippet: First-strand cDNA was used as template for subsequent PCR analysis in a 50 µl total volume composed of 5 µl 10x Taq buffer (Fermentas/Thermo Scientific), 2 µl 25 mM MgCl2 (Fermentas/Thermo Scientific), 2 µl 10mM deoxyribonucleotide triphosphates (dNTPs; Fermentas/Thermo Scientific), and 1 µl 5U/µL Taq polymerase (Fermentas/Thermo Scientific) in RNase-free water. .. Plasmid specific primers as described by Okita et al., 2011 [ ] were used for RT-PCR ( ).

    RNA Extraction:

    Article Title: Generation of transgene-free porcine intermediate type induced pluripotent stem cells
    Article Snippet: Paragraph title: RNA extraction and reverse transcription PCR (RT-PCR) ... First-strand cDNA was used as template for subsequent PCR analysis in a 50 µl total volume composed of 5 µl 10x Taq buffer (Fermentas/Thermo Scientific), 2 µl 25 mM MgCl2 (Fermentas/Thermo Scientific), 2 µl 10mM deoxyribonucleotide triphosphates (dNTPs; Fermentas/Thermo Scientific), and 1 µl 5U/µL Taq polymerase (Fermentas/Thermo Scientific) in RNase-free water.

    Agarose Gel Electrophoresis:

    Article Title: Glial Fibrillary Acidic Protein–Apolipoprotein E (apoE) Transgenic Mice: Astrocyte-Specific Expression and Differing Biological Effects of Astrocyte-Secreted apoE3 and apoE4 Lipoproteins
    Article Snippet: The PCR reaction was in 50 μl and contained 2 μl of DNA (∼50 ng), 2.5 μl of each primer (10 μ m stock solution), 10× PCR buffer (20 m m Tris-HCl, pH 8.0, 0.1 m m EDTA, 1 m m DTT, and 50% glycerol), 200 μ m dNTP, and 0.25 μl of Taq polymerase (5 U/μl; Life Technologies, Grand Island, NY). .. The PCR reaction was in 50 μl and contained 2 μl of DNA (∼50 ng), 2.5 μl of each primer (10 μ m stock solution), 10× PCR buffer (20 m m Tris-HCl, pH 8.0, 0.1 m m EDTA, 1 m m DTT, and 50% glycerol), 200 μ m dNTP, and 0.25 μl of Taq polymerase (5 U/μl; Life Technologies, Grand Island, NY).

    Produced:

    Article Title: Glacial Runoff Promotes Deep Burial of Sulfur Cycling-Associated Microorganisms in Marine Sediments
    Article Snippet: Barcoded 16S rRNA gene amplicons were produced with a two-step PCR barcoding approach , using the general bacterial and archaeal primers U519F (5′-CAGCMGCCGCGGTAATWC-3′) and 802R (5′-TACNVGGGTATCTAATCC-3′) for initial amplification ( ). .. The reaction mix contained 1 × Taq buffer (Thermo Scientific), 0.2 mM dNTP mix (Thermo Scientific), 2 mM MgCl2 , 0.25 U Taq polymerase (Thermo Scientific), 0.2 μM of each primer, and approximately 1−10 ng DNA.

    Concentration Assay:

    Article Title: Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
    Article Snippet: .. A 919 bp product spanning the prnp coding region was amplified from each elk sample in a 100 μl reaction using the following conditions (final concentration in brackets): 2.5 U Taq polymerase (Invitrogen), 10x buffer (1×), 25 mM dNTPs (2 mM), 50 mM MgCl2 (2.5 mM), 5 μM forward primer (0.5 μM), 5 μM reverse primer (0.5 μM), 100 ng of DNA (1 ng/μl). .. The PCR conditions were as follows: 5 minute denaturing phase at 94 °C, followed by 36 cycles of: 1 minute denaturing at 94 °C; 30 seconds annealing at 64.5 °C; 1 minute extension at 72 °C; with a final extension step of 72 °C for 10 minutes.

    DNA Purification:

    Article Title: Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas
    Article Snippet: The homogenates were digested by proteinase K at 55 °C for 3 h and processed for DNA extraction using Wizard® Genomic DNA Purification Kit (Promega, USA) as instructed by the manufacturer. .. For B2L gene amplification, PCR was performed in a thermocycler using 2 µl of DNA as template in a 25 μl reaction volume containing 10 pmol of each primer and 1 U Taq DNA polymerase (Fermentas, USA).

    Staining:

    Article Title: Homeostasis of Retinol in Lecithin:retinol Acyltransferase Gene Knockout Mice Fed a High Retinol Diet
    Article Snippet: The PCR was performed using the following conditions: 94 °C for 30 s, 58 °C for 30 s, and 72 °C for 80 s, with a final extension at 72 °C for 10 min. Taq polymerase was from Invitrogen (catalog number 18038–042). .. The gel images, stained with ethidium bromide, were recorded with a FluorChem 8800 system (Alpha Innotech, San Leandro, CA).

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    Thermo Fisher taq dna polymerase
    A mobility shift assay for TaqS <t>DNA</t> polymerase (A) and NeqSSB-TaqS DNA polymerase (B) with ssDNA and dsDNA. The output products were analyzed on a 2% agarose gel with ethidium bromide in the UV light. The reaction mix contained 10 pmol Oligo (dT) 76 and/or 2.5 pmol PCR product with a length of 100 bp. In panel A: 1. Oligo (dT) 76 and 0 pmol TaqS DNA polymerase; 2. 100 bp PCR product and 0 pmol DNA polymerase; 3–9. Oligo (dT) 76 and 100 bp PCR product with 24,6; 49,2; 98,4; 196,8; 393,6; 787,2; 1574,4 pmol of TaqS DNA polymerase, respectively. In panel B: 11. Oligo (dT) 76 and 0 pmol NeqSSB-TaqS DNA polymerase. 12. 100 bp PCR product and 0 pmol NeqSSB-TaqS DNA polymerase. 13–19. Oligo (dT) 76 and 100 bp PCR product with 3,3; 6,6; 13,2; 26,4; 52,8; 105,6; 211,2 pmol Neq SSB- <t>Taq</t> S DNA polymerase, respectively.
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A mobility shift assay for TaqS DNA polymerase (A) and NeqSSB-TaqS DNA polymerase (B) with ssDNA and dsDNA. The output products were analyzed on a 2% agarose gel with ethidium bromide in the UV light. The reaction mix contained 10 pmol Oligo (dT) 76 and/or 2.5 pmol PCR product with a length of 100 bp. In panel A: 1. Oligo (dT) 76 and 0 pmol TaqS DNA polymerase; 2. 100 bp PCR product and 0 pmol DNA polymerase; 3–9. Oligo (dT) 76 and 100 bp PCR product with 24,6; 49,2; 98,4; 196,8; 393,6; 787,2; 1574,4 pmol of TaqS DNA polymerase, respectively. In panel B: 11. Oligo (dT) 76 and 0 pmol NeqSSB-TaqS DNA polymerase. 12. 100 bp PCR product and 0 pmol NeqSSB-TaqS DNA polymerase. 13–19. Oligo (dT) 76 and 100 bp PCR product with 3,3; 6,6; 13,2; 26,4; 52,8; 105,6; 211,2 pmol Neq SSB- Taq S DNA polymerase, respectively.

    Journal: PLoS ONE

    Article Title: Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization

    doi: 10.1371/journal.pone.0184162

    Figure Lengend Snippet: A mobility shift assay for TaqS DNA polymerase (A) and NeqSSB-TaqS DNA polymerase (B) with ssDNA and dsDNA. The output products were analyzed on a 2% agarose gel with ethidium bromide in the UV light. The reaction mix contained 10 pmol Oligo (dT) 76 and/or 2.5 pmol PCR product with a length of 100 bp. In panel A: 1. Oligo (dT) 76 and 0 pmol TaqS DNA polymerase; 2. 100 bp PCR product and 0 pmol DNA polymerase; 3–9. Oligo (dT) 76 and 100 bp PCR product with 24,6; 49,2; 98,4; 196,8; 393,6; 787,2; 1574,4 pmol of TaqS DNA polymerase, respectively. In panel B: 11. Oligo (dT) 76 and 0 pmol NeqSSB-TaqS DNA polymerase. 12. 100 bp PCR product and 0 pmol NeqSSB-TaqS DNA polymerase. 13–19. Oligo (dT) 76 and 100 bp PCR product with 3,3; 6,6; 13,2; 26,4; 52,8; 105,6; 211,2 pmol Neq SSB- Taq S DNA polymerase, respectively.

    Article Snippet: The processivity values determined with the use of a heparin trap were much lower than the values previously published for the Taq DNA polymerase [ , ] and were within a range of 80 nt to 160 nt.

    Techniques: Mobility Shift, Agarose Gel Electrophoresis, Polymerase Chain Reaction

    Evaluation of PCR amplification rate. Comparison of the PCR amplification rates of a fusion Neq SSB -TaqS DNA polymerase for 300 bp (A), 500 bp (B), 1000 bp (C) products and a Taq S DNA polymerase for 300 bp (D), 500 bp (E), 1000 bp (F) products. The elongation times used for the PCR amplification are indicated at the top. Lane M: the DNA molecular size marker (50–2000 bp).

    Journal: PLoS ONE

    Article Title: Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization

    doi: 10.1371/journal.pone.0184162

    Figure Lengend Snippet: Evaluation of PCR amplification rate. Comparison of the PCR amplification rates of a fusion Neq SSB -TaqS DNA polymerase for 300 bp (A), 500 bp (B), 1000 bp (C) products and a Taq S DNA polymerase for 300 bp (D), 500 bp (E), 1000 bp (F) products. The elongation times used for the PCR amplification are indicated at the top. Lane M: the DNA molecular size marker (50–2000 bp).

    Article Snippet: The processivity values determined with the use of a heparin trap were much lower than the values previously published for the Taq DNA polymerase [ , ] and were within a range of 80 nt to 160 nt.

    Techniques: Polymerase Chain Reaction, Amplification, Marker

    Characterization of a fusion Neq SSB -TaqS DNA polymerase in comparison to a Taq S DNA polymerase. The effect of (A) MgCl 2 , (B) KCl, (C) (NH 4 ) 2 SO 4 , (D) pH and (E) temperature on the polymerase activity. The results for the NeqSSB-TaqS DNA polymerase are marked with black circles, whilst for the Taq S DNA polymerase with black tringles. Error bars for the TaqS DNA polymerase have the end bar whilst for the Neq - Taq S DNA polymerase does not have the end bar.

    Journal: PLoS ONE

    Article Title: Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization

    doi: 10.1371/journal.pone.0184162

    Figure Lengend Snippet: Characterization of a fusion Neq SSB -TaqS DNA polymerase in comparison to a Taq S DNA polymerase. The effect of (A) MgCl 2 , (B) KCl, (C) (NH 4 ) 2 SO 4 , (D) pH and (E) temperature on the polymerase activity. The results for the NeqSSB-TaqS DNA polymerase are marked with black circles, whilst for the Taq S DNA polymerase with black tringles. Error bars for the TaqS DNA polymerase have the end bar whilst for the Neq - Taq S DNA polymerase does not have the end bar.

    Article Snippet: The processivity values determined with the use of a heparin trap were much lower than the values previously published for the Taq DNA polymerase [ , ] and were within a range of 80 nt to 160 nt.

    Techniques: Activity Assay

    Effect of various ribonucleotide substitutions on iLock probe RNA detection assay with PBCV-1 ligase. Recognition of the invader structure and structure-specific endonucleolytic activity of Taq DNA polymerase can vary for different RNA substitutions. ( A ) Targeting let-7a with iLock probe. Ribonucleotides were introduced: at a terminal 3′ base (3); base in the 5′ arm, that an invading 3′ arm competes with for target binding (displaced base, 3D); base in the 5′ arm, that becomes a 5′-phosphorylated donor after iLock probe activation (3D5); in the flap sequence (3DF/DF). ( B ) iLocks were modified according to A , except nonchimeric iLock (DNA). The total number of RCPs for each probe is shown on y -axis. ( C ) PAGE of three selected iLock probes (DNA, 3, 3D) after activation and ligation, without (first three lanes) and with Taq DNA polymerase (last three lanes). Nonactivated iLock probe (79) is shortened upon activation by 14 nt (65) and ligated (seen as high molecular weight band at the top of the gel). (22) let-7a miRNA.

    Journal: RNA

    Article Title: Chimeric padlock and iLock probes for increased efficiency of targeted RNA detection

    doi: 10.1261/rna.066753.118

    Figure Lengend Snippet: Effect of various ribonucleotide substitutions on iLock probe RNA detection assay with PBCV-1 ligase. Recognition of the invader structure and structure-specific endonucleolytic activity of Taq DNA polymerase can vary for different RNA substitutions. ( A ) Targeting let-7a with iLock probe. Ribonucleotides were introduced: at a terminal 3′ base (3); base in the 5′ arm, that an invading 3′ arm competes with for target binding (displaced base, 3D); base in the 5′ arm, that becomes a 5′-phosphorylated donor after iLock probe activation (3D5); in the flap sequence (3DF/DF). ( B ) iLocks were modified according to A , except nonchimeric iLock (DNA). The total number of RCPs for each probe is shown on y -axis. ( C ) PAGE of three selected iLock probes (DNA, 3, 3D) after activation and ligation, without (first three lanes) and with Taq DNA polymerase (last three lanes). Nonactivated iLock probe (79) is shortened upon activation by 14 nt (65) and ligated (seen as high molecular weight band at the top of the gel). (22) let-7a miRNA.

    Article Snippet: Duplicate reactions were incubated in a heated-lid thermocycler at 51°C for 30 min, in a 10 µL volume containing 0.1 U/µL of Taq DNA polymerase (ThermoFisher Scientific), 0.4 U/µL U RNase Inhibitor (Blirt) and 1× Taq polymerase buffer supplied with 8 mM MgCl2.

    Techniques: RNA Detection, Activity Assay, Binding Assay, Activation Assay, Sequencing, Modification, Polyacrylamide Gel Electrophoresis, Ligation, Molecular Weight