taq polymerase  (TaKaRa)


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    Name:
    Titanium Taq DNA Polymerase
    Description:
    Titanium Taq DNA Polymerase is a blend of a specially engineered Taq and an antibody for integrated hot start PCR which prevents non specific amplification and primer dimer formation Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples including bacterial and plasmid DNA cDNA and complex genomic DNA It gives higher PCR product yield than other polymerases and is active over a wide range of Mg2 concentrations Titanium Taq is available in several formats
    Catalog Number:
    639242
    Price:
    None
    Category:
    Titanium Taq DNA Polymerase Titanium Taq products High yield PCR PCR
    Size:
    1 000 Rxns
    		Buy from Supplier

    Structured Review

    TaKaRa taq polymerase
    Titanium Taq DNA Polymerase is a blend of a specially engineered Taq and an antibody for integrated hot start PCR which prevents non specific amplification and primer dimer formation Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples including bacterial and plasmid DNA cDNA and complex genomic DNA It gives higher PCR product yield than other polymerases and is active over a wide range of Mg2 concentrations Titanium Taq is available in several formats
    https://www.bioz.com/result/taq polymerase/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taq polymerase - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Modification:

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: .. Indeed, our recent study showed that TITANIUM Taq DNA pol exhibited high selectivity and efficiency in a one-way incorporation of modified d Px TP opposite Ds in templates, and is useful for the unnatural base incorporation in a primer region in PCR ( ). .. These results suggest that the TITANIUM Taq and Taq DNA pols efficiently incorporate d Px TP into DNA opposite Ds , but are not useful to incorporate d Ds TP into DNA opposite Px .

    Polymerase Chain Reaction:

    Article Title: Highly specific unnatural base pair systems as a third base pair for PCR amplification
    Article Snippet: .. Indeed, our recent study showed that TITANIUM Taq DNA pol exhibited high selectivity and efficiency in a one-way incorporation of modified d Px TP opposite Ds in templates, and is useful for the unnatural base incorporation in a primer region in PCR ( ). .. These results suggest that the TITANIUM Taq and Taq DNA pols efficiently incorporate d Px TP into DNA opposite Ds , but are not useful to incorporate d Ds TP into DNA opposite Px .

    Article Title: Contributions of MET activation to BCR-ABL1 tyrosine kinase inhibitor resistance in chronic myeloid leukemia cells
    Article Snippet: Array-CGHThe Genome-wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA) was used to perform array-CGH on genomic DNA from each of the cell lines, in accordance with the manufacturer's instructions. .. A total of 250 ng of genomic DNA was digested with the restriction enzymes Nsp I and Sty I in independent parallel reactions (SNP6.0), ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech). .. The PCR products were quantified, fragmented, end-labeled, and hybridized onto a Genome-wide Human SNP Array 6.0.

    Article Title: The identification of additional zebrafish DICP genes reveals haplotype variation and linkage to MHC class I genes
    Article Snippet: Amplicons were cloned into the pGEM®-T Easy plasmid and sequenced. .. Haplotype analysesTo investigate a predicted alternative DICP haplotype, PCR was performed using genomic DNA from adult zebrafish, the DICP1.1 and DICP1.22 primer pairs, Titanium Taq DNA polymerase and the cycling parameters described in . .. Genomic DNA was obtained from fin clips of the adult zebrafish described above using a modified HotSHOT protocol ( ).

    Article Title: Bestrophin-Encoded Ca2+-Activated Cl− Channels Underlie a Current with Properties Similar to the Native Current in the Moth Spodoptera littoralis Olfactory Receptor Neurons
    Article Snippet: A multiplex PCR (PCR1) was first carried out for the simultaneous amplification of S. littoralis bestrophins and two control genes (RpL8 and SlitOrco). .. Sixty microliters of a PCR mix containing 10 mM dNTPs Mix (2 µl), 2 µl 50× Titanium Taq DNA polymerase and 10 µl of 10× PCR buffer (Clontech) were added to the RT product with 20 µl of a mix containing the sense and antisense primers (10 pmol each) (SlitBest1a-SC.F/SlitBest1-SC.R1, SlitBest1b-SC.F/SlitBest1-SC.R1, SlitBest2-SC.F/SlitBest2-SC.R1, SlitOrco-SC.F/SlitOrco-SC.R1, RpL8-SC.F/RpL8-SC.R1 primers are shown in ). .. After 1 min at 94°C, samples were processed for 35 cycles of 95°C for 30 s, 60°C for 30 s, and 68°C for 1 min. Then, a nested PCR was performed on 2 µl of PCR1 products with 46 µl of a reaction mix containing 5 µl 10 X Titanium Taq buffer, 1 µl 50 X Titanium Taq DNA polymerase, and 1 µl of dNTPs (10 mM) and an antisense primer specific to each gene (SlitBest1-SC.R2, SlitBest2-SC.R2, SlitOrco-SC.R2 and RpL8-SC.R2).

    Article Title: Combined megaplex TCR isolation and SMART-based real-time quantitation methods for quantitating antigen-specific T cell clones in mycobacterial infection
    Article Snippet: The lysate was incubated at 55 °C for 1.5 h followed by heat inactivation of the proteinase K at 99 °C for 10 min. DNA samples were then kept at −20 °C until use for PCR reactions. .. Primary PCR amplification was performed in a single tube with 50 μl volume containing mixtures of ext.Vβ family-specific primers (24 families, 0.25 μM each), mixtures of ext.Jβ-specific primers (13 segments, 0.25 μM each), 1 × Titanium DNA Polymerase (Clontech) and 12–20 μl of DNA template sample. ..

    Article Title: The identification of additional zebrafish DICP genes reveals haplotype variation and linkage to MHC class I genes
    Article Snippet: PCR primer pairs were designed by Primer 3 software ( ) to amplify the Ig domains of several related members of the DICP family and to span at least one intron ( and ). .. Relative gene expression levels were determined by PCR using TITANIUM™ Taq DNA polymerase (Clontech, Mountain View, CA); annealing temperatures, extension times and number of cycles for each primer pair are listed in . ..

    Amplification:

    Article Title: Contributions of MET activation to BCR-ABL1 tyrosine kinase inhibitor resistance in chronic myeloid leukemia cells
    Article Snippet: Array-CGHThe Genome-wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA) was used to perform array-CGH on genomic DNA from each of the cell lines, in accordance with the manufacturer's instructions. .. A total of 250 ng of genomic DNA was digested with the restriction enzymes Nsp I and Sty I in independent parallel reactions (SNP6.0), ligated to the adaptor, and amplified using PCR with a universal primer and TITANIUM Taq DNA Polymerase (Clontech). .. The PCR products were quantified, fragmented, end-labeled, and hybridized onto a Genome-wide Human SNP Array 6.0.

    Article Title: Combined megaplex TCR isolation and SMART-based real-time quantitation methods for quantitating antigen-specific T cell clones in mycobacterial infection
    Article Snippet: The lysate was incubated at 55 °C for 1.5 h followed by heat inactivation of the proteinase K at 99 °C for 10 min. DNA samples were then kept at −20 °C until use for PCR reactions. .. Primary PCR amplification was performed in a single tube with 50 μl volume containing mixtures of ext.Vβ family-specific primers (24 families, 0.25 μM each), mixtures of ext.Jβ-specific primers (13 segments, 0.25 μM each), 1 × Titanium DNA Polymerase (Clontech) and 12–20 μl of DNA template sample. ..

    Expressing:

    Article Title: The identification of additional zebrafish DICP genes reveals haplotype variation and linkage to MHC class I genes
    Article Snippet: PCR primer pairs were designed by Primer 3 software ( ) to amplify the Ig domains of several related members of the DICP family and to span at least one intron ( and ). .. Relative gene expression levels were determined by PCR using TITANIUM™ Taq DNA polymerase (Clontech, Mountain View, CA); annealing temperatures, extension times and number of cycles for each primer pair are listed in . ..

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    • la dna polymerase  (TaKaRa)
    99
    TaKaRa la dna polymerase
    ( A ) Gel electrophoresis of near full-length genome PCR products produced from four long amplifying <t>DNA</t> polymerases; KlenTaq LA (discontinued), AccuTaq LA, PrimeSTAR GXL and <t>Takara</t> LA Taq (separate gel) per manufacturer’s instructions for samples with high GC/secondary structures. M, HyperLadder 1 kb; 1, CVB3 Nancy; 2, CVB5 Faulkner; 3, H 2 O control. ( B ) Gel electrophoresis of near full-length genome PCR products produced from Takara LA Taq DNA polymerase. M, HyperLadder 1 kb; 1–4, known EV positives from NSW Health Pathology East virology diagnostic lab; 5, CVB3 Nancy; 6, H 2 .
    La Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la dna polymerase/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    la dna polymerase - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier

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    ( A ) Gel electrophoresis of near full-length genome PCR products produced from four long amplifying DNA polymerases; KlenTaq LA (discontinued), AccuTaq LA, PrimeSTAR GXL and Takara LA Taq (separate gel) per manufacturer’s instructions for samples with high GC/secondary structures. M, HyperLadder 1 kb; 1, CVB3 Nancy; 2, CVB5 Faulkner; 3, H 2 O control. ( B ) Gel electrophoresis of near full-length genome PCR products produced from Takara LA Taq DNA polymerase. M, HyperLadder 1 kb; 1–4, known EV positives from NSW Health Pathology East virology diagnostic lab; 5, CVB3 Nancy; 6, H 2 .

    Journal: Scientific Reports

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples

    doi: 10.1038/s41598-018-30322-y

    Figure Lengend Snippet: ( A ) Gel electrophoresis of near full-length genome PCR products produced from four long amplifying DNA polymerases; KlenTaq LA (discontinued), AccuTaq LA, PrimeSTAR GXL and Takara LA Taq (separate gel) per manufacturer’s instructions for samples with high GC/secondary structures. M, HyperLadder 1 kb; 1, CVB3 Nancy; 2, CVB5 Faulkner; 3, H 2 O control. ( B ) Gel electrophoresis of near full-length genome PCR products produced from Takara LA Taq DNA polymerase. M, HyperLadder 1 kb; 1–4, known EV positives from NSW Health Pathology East virology diagnostic lab; 5, CVB3 Nancy; 6, H 2 .

    Article Snippet: First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U).

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Produced, Diagnostic Assay

    ( A ) Gel electrophoresis of near full-length genome PCR products produced from four long amplifying DNA polymerases; KlenTaq LA (discontinued), AccuTaq LA, PrimeSTAR GXL and Takara LA Taq (separate gel) per manufacturer’s instructions for samples with high GC/secondary structures. M, HyperLadder 1 kb; 1, CVB3 Nancy; 2, CVB5 Faulkner; 3, H 2 O control. ( B ) Gel electrophoresis of near full-length genome PCR products produced from Takara LA Taq DNA polymerase. M, HyperLadder 1 kb; 1–4, known EV positives from NSW Health Pathology East virology diagnostic lab; 5, CVB3 Nancy; 6, H 2 O control. Full-length gels are presented in Supplementary Fig. 1 .

    Journal: Scientific Reports

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples

    doi: 10.1038/s41598-018-30322-y

    Figure Lengend Snippet: ( A ) Gel electrophoresis of near full-length genome PCR products produced from four long amplifying DNA polymerases; KlenTaq LA (discontinued), AccuTaq LA, PrimeSTAR GXL and Takara LA Taq (separate gel) per manufacturer’s instructions for samples with high GC/secondary structures. M, HyperLadder 1 kb; 1, CVB3 Nancy; 2, CVB5 Faulkner; 3, H 2 O control. ( B ) Gel electrophoresis of near full-length genome PCR products produced from Takara LA Taq DNA polymerase. M, HyperLadder 1 kb; 1–4, known EV positives from NSW Health Pathology East virology diagnostic lab; 5, CVB3 Nancy; 6, H 2 O control. Full-length gels are presented in Supplementary Fig. 1 .

    Article Snippet: Amplification of Near Full-Length EV Genomes First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U).

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Produced, Diagnostic Assay

    Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify PCR products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a DNA template for each PCR reaction.

    Journal: PLoS ONE

    Article Title: Chimpanzee-Specific Endogenous Retrovirus Generates Genomic Variations in the Chimpanzee Genome

    doi: 10.1371/journal.pone.0101195

    Figure Lengend Snippet: Alternative PNRC2 transcripts caused by a chimpanzee-specific ERV. (A) Using the UCSC Genome Browser, the PNRC2 gene is anatomized into intron, un-translated region (UTR), and coding exon. (B) The structures of chimpanzee and non-chimpanzee primate PNRC2 s and their transcripts are depicted. The black and gray boxes indicate an exon and UTRs, respectively, and the red box denotes the untranscribed UTR1 of the chimpanzee PNRC2 . Four different primers used to amplify PCR products are shown in red, blue, green, and black arrows, respectively. The target site duplications (TSDs) of the full-length chimpanzee-specific ERV are written in red. (C) The picture shows an agarose gel image of PCR products. Genomic DNAs from four different primates (human, chimpanzee, gorilla, and orangutan) were used as a DNA template for each PCR reaction.

    Article Snippet: We designed oligonucleotide primers for each PCR reaction by using the software Primer3 ( and ) and PCR amplification of each chimpanzee-specific ERV was performed in 25 µL reaction using 10-20 ng DNA, 200 nM of each oligonucleotide primer, and 10 µL of PCR master mix containing DNA polymerase (TaKaRa EmeraldAmp GT PCR Master Mix, TaKaRa, Japan).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Expression of DUSP6 in immortalized and cancer cell lines. Copy numbers of DUSP6 and ACTB mRNA were measured by quantitative reverse transcription-PCR. The means and standard deviations (error bars) of the mRNA expression of DUSP6 normalized to that of

    Journal: The American Journal of Pathology

    Article Title: Down-Regulation of DUSP6 Expression in Lung Cancer --Its Mechanism and Potential Role in Carcinogenesis

    doi: 10.2353/ajpath.2009.080489

    Figure Lengend Snippet: Expression of DUSP6 in immortalized and cancer cell lines. Copy numbers of DUSP6 and ACTB mRNA were measured by quantitative reverse transcription-PCR. The means and standard deviations (error bars) of the mRNA expression of DUSP6 normalized to that of

    Article Snippet: The region from −960 to −266 of the DUSP6 gene, including the promoter locus, was PCR-amplified (TaqHS DNA polymerase, Takara) with three sets of primers (F1, 5′-GAGTTGGGTTTTTAAAGTGGTAAATA-3′ and R1, 5′-CAAAACACATAAACCAAAACACTTC-3′; F2, 5′-GAAATATGAGATAATTGAAGTGTTTTGG-3′ and R2, 5′-CAACTCCTCAATAAATACAAACAAC-3′; F3, 5′-TGTTTGTATTTATTGAGGAGTTGTTT-3′ and R3, 5′-CTACCCAAATAATTTTTATTCCTCC-3′), and subcloned into pT7Blue (Novagen).

    Techniques: Expressing, Polymerase Chain Reaction

    Methylation status of the DUSP6 promoter and intron 1. Cells treated with vehicle (Vcl), AZA, TSA, or a combination of AZA and TSA were examined for restoration of DUSP6 mRNA expression by quantitative reverse transcription-PCR. The copy number of DUSP6

    Journal: The American Journal of Pathology

    Article Title: Down-Regulation of DUSP6 Expression in Lung Cancer --Its Mechanism and Potential Role in Carcinogenesis

    doi: 10.2353/ajpath.2009.080489

    Figure Lengend Snippet: Methylation status of the DUSP6 promoter and intron 1. Cells treated with vehicle (Vcl), AZA, TSA, or a combination of AZA and TSA were examined for restoration of DUSP6 mRNA expression by quantitative reverse transcription-PCR. The copy number of DUSP6

    Article Snippet: The region from −960 to −266 of the DUSP6 gene, including the promoter locus, was PCR-amplified (TaqHS DNA polymerase, Takara) with three sets of primers (F1, 5′-GAGTTGGGTTTTTAAAGTGGTAAATA-3′ and R1, 5′-CAAAACACATAAACCAAAACACTTC-3′; F2, 5′-GAAATATGAGATAATTGAAGTGTTTTGG-3′ and R2, 5′-CAACTCCTCAATAAATACAAACAAC-3′; F3, 5′-TGTTTGTATTTATTGAGGAGTTGTTT-3′ and R3, 5′-CTACCCAAATAATTTTTATTCCTCC-3′), and subcloned into pT7Blue (Novagen).

    Techniques: Methylation, Expressing, Polymerase Chain Reaction